• International Journal of Precision Engineering and Manufacturing
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• v.8 no.3
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• pp.60-63
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• 2007

Weight functions in fracture mechanics represent the stress intensity factors as weighted averages of the externally impressed boundary tractions and body forces. We extended the weight function theory for cracked linear elastic materials to calculate the notch stress intensity factor of a notched structure with anti-plane deformation. The well-known method of deriving weight functions by differentiation cannot be used for notched structures. By combining an appropriate singular field with a regular field, we derived weight functions for the notch stress intensity factor. Closed expressions of weight functions for notched cylindrical bodies are given as examples.

• Development and Reproduction
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• v.7 no.2
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• pp.69-80
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• 2003

The zebrafish(Danio rerio) is a pre-eminent vertebrate model system for clarification of the roles of specific genes and signaling pathways in development. We show examples of positional cloning in two developmental mutants in zebrafish. headless: The severe head defects in headless(hdl) mutants are due to a mutation in T-cell factor-3(Tcf3). Loss of Tcf3 function in the hdl mutant reveals that Hdl represses Wnt target genes. The results provide genetic evidence that a component of the Wnt signaling pathway is essential in vertebrate head formation and patterning. mind bomb: Reduced lateral inhibition in mind bomb(mib) mutants permits too many neural precursors to differentiate as neurons. Positional cloning of mib revealed that it is a gene in the Notch pathway that encodes a ubiquitin E3 ligase. Mib interacts with the intracellular domain of Delta to promote its internalization. The results suggest a model for Notch activation where the Delta-Notch interaction is followed by endocytosis of Delta and transendocytosis of the Notch extracellular domain by the signaling cell.

• Molecules and Cells
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• v.40 no.1
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• pp.73-81
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• 2017

The ${\gamma}$-secretase complex represents an evolutionarily conserved family of transmembrane aspartyl proteases that cleave numerous type-I membrane proteins, including the ${\beta}$-amyloid precursor protein (APP) and the receptor Notch. All known rare mutations in APP and the ${\gamma}$-secretase catalytic component, presenilin, which lead to increased amyloid ${\beta}$-peptide production, are responsible for early-onset familial Alzheimer's disease. ${\beta}$-amyloid protein precursor-like (APPL) is the Drosophila ortholog of human APP. Here, we created Notch- and APPL-based Drosophila reporter systems for in vivo monitoring of ${\gamma}$-secretase activity. Ectopic expression of the Notch- and APPL-based chimeric reporters in wings results in vein truncation phenotypes. Reporter-mediated vein truncation phenotypes are enhanced by the Notch gain-of-function allele and suppressed by RNAi-mediated knockdown of presenilin. Furthermore, we find that apoptosis partly contributes to the vein truncation phenotypes of the APPL-based reporter, but not to the vein truncation phenotypes of the Notch-based reporter. Taken together, these results suggest that both in vivo reporter systems provide a powerful genetic tool to identify genes that modulate ${\gamma}$-secretase activity and/or APPL metabolism.

• Computers and Concrete
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• v.24 no.6
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• pp.527-539
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• 2019

In this paper, the fracture characteristics of concrete specimens with different notch depths under three-point flexural loads are studied by finite element and fracture mechanics methods. Firstly, the concrete beams (the size is 700×100×150 mm) with different notch depths (a=30 mm, 45 mm, 60 mm and 75 mm respectively) are tested to study the influence of notch depths on the mechanical properties of concrete. Subsequently, the concrete beams with notch depth of 60 mm are loaded at different loading rates to study the influence of loading rates on the fracture characteristics, and digital image correlation (DIC) is used to monitor the strain nephogram at different loading rates. The test results show that the flexural characteristics of the beams are influenced by notch depths, and the bearing capacity and ductility of the concrete decrease with the increase of notch depths. Moreover, the peak load of concrete beam gradually increases with the increase of loading rate. Then, the fracture energy of the beams is accurately calculated by tail-modeling method and the bilinear softening constitutive model of fracture behavior is determined by using the modified fracture energy. Finally, the bilinear softening constitutive function is embedded into the finite element (FE) model for numerical simulation. Through the comparison of the test results and finite element analysis, the bilinear softening model determined by the tail-modeling method can be used to predict the fracture behavior of concrete beams under different notch depths and loading rates.

• Journal of the Korean Ceramic Society
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• v.34 no.10
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• pp.1053-1059
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• 1997

The dynamic fatigue behavior of alumina ceramics was observed at room temperature using four point bending system. The dynamic fatigue fracture strength and the dynamic fatigue lifetime were observed as a function of crosshead speed and the notch length. The notched specimen showed the smaller deviation in dynamic fatigue fracture strength than the unnotched specimen. The crack growth exponent n and the material constant A of the notched specimen could be represented as functions of the notch length. Fracture strength of the specimen calculated from the notch length, when the notch length was regarded as the crack size, was in good agreement with the measured 4 point bending strength. Fracture surface of the specimen showed the different fracture modes according to the crosshead speed. The four point flexural strength, fracture toughness, Young's modulus and Weibull modulus of the alumina were measured as 360 MPa, 3.91 MPa.m1/2, 159GPa, 17.64, respectively.

• Journal of Mechanical Science and Technology
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• v.17 no.11
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• pp.1659-1664
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• 2003

A cracked plate with a patch bonded on one side was treated with a crack-bridging model using weight function: assuming continuous distribution of springs acting between th crack surfaces, the stress intensity factor of the patched crack was numerically obtained. Especially in the case of a patched crack subjected to residual non-uniform stress, the stress intensity factor was easily with the corresponding approximate weight function. This paper presented the stress intensity factors for a crack partially patched within a finite plate or a patched crack initiated from a notch.

• Journal of Welding and Joining
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• v.2 no.1
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• pp.41-48
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• 1984

Recrystallization technique was applied to analyze plastic strain at the notch tip of coarse grain HAZ in mild steel (SB 41) and high strength steel (SA 588). The notch tip of specimen was deformed by three point bending. Accumulated displacement (Crack Opening Displacement ${delta}t$) by the monotonic and cyclic loading under room temperature and hot strain embrittlement temperature ($250^{\circ}C$) was 0~1.0mm. Recrystallization heat treatment conditions were $650^{circ}C{ imes}3hr$ for SB 41 and $700^{circ}C{ imes}3hr$ for SA 588. The experimental results obtained were as follows ; 1) Distribution of the effective plastic strain at plastic zone was appeared by the function of crack opening displacement, and plastic zone or the effective plastic strain increased with crack opening displacement. 2) Plastic strain at notch tip of HAZ due to accumulated hot strain calculated as follows. .epsilon. over bar $_{p}$ = .epsilon. over bar $_{cr}$ (x/ $R_{x}$ ) $^{m}$ (m=0.25) 3) Work hardending ratio of notch tip for hot strain was linearly increased with .epsilon. over bar $_{max}$ and dependent upon the material types.s.

• BMB Reports
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• v.50 no.1
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• pp.1-2
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• 2017

Acquiring a selective growth advantage by breaking the proliferation barrier established by gatekeeper genes is a centrally important event in tumor formation. Removal of the mammalian Hippo kinase Mst1 and Mst2 in hepatocytes leads to rapid hepatocellular carcinoma (HCC) formation, indicating that the Hippo signaling pathway is a critical gatekeeper that restrains abnormal growth in hepatocytes. By rigorous genetic approaches, we identified an interacting network of the Hippo, Wnt/${\beta}$-catenin and Notch signaling pathways that control organ size and HCC development. We found that in hepatocytes, the loss of Mst1/2 leads to the activation of Notch signaling, which forms a positive feedback loop with Yap/Taz (transcription factors controlled by Mst1/2). This positive feedback loop results in severe liver enlargement and rapid HCC formation. Blocking the Yap/Taz-Notch positive feedback loop by Notch inhibition in vivo significantly reduced the Yap/Taz activities, hepatocyte proliferation and tumor formation. Furthermore, we uncovered a surprising inhibitory role of Wnt/${\beta}$-catenin signaling to Yap/Taz activities, which are important in tumor initiation. Genetic removal of ${\beta}$-catenin in the liver of the Mst1/2 mutants significantly accelerates tumoriogenesis. Therefore, Wnt/${\beta}$-catenin signaling, known for its oncogenic property, exerts an unexpected function in restricting Yap/Taz and Notch activities in HCC initiation. The molecular interplay between the three signaling pathways identified in our study provides new insights in developing novel therapeutic strategies to treat liver tumors.

• Journal of Magnetics
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• v.13 no.4
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• pp.136-139
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• 2008

A simplified equation of depinning fields from notches of ferromagnetic Permalloy nanowires is presented. The derived equation is given in the form of an explicit function of nanowire width and thickness, and notch depth and angle. The equation agrees with all micromagnetic simulation results to an accuracy of ${\pm}$ 0.5 mT.

• Proceedings of the Korean Society of Precision Engineering Conference
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• 2006.05a
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• pp.271-272
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• 2006

This paper presented a new experimental gain tuning technique for a Multi-Axis Servo System. First, the investigation for proportional gain of velocity control loop by using a Dynamic Signal Analyzer (DSA) was performed. Using the FUNCTION characteristic of DSA based on the Bode plot, the Bode plot of open loop transfer function was obtained. In turn, the integral gain of a servo controller can be found out by using the Integration time constant extracted from the Bode plot of open loop transfer function. In the meanwhile, the positional gain of the servo controller has been obtained by using the Bode plot of the closed loop transfer function. We have also proposed the technique to find out an optimal parameter of a notch filter, which has a great influence on vibration reduction, by using the damping factor extracted from the Bode plot of closed loop transfer function.