• Title/Summary/Keyword: northern plants

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Vascular Plants Distributed in Ridge of the Northernmost Baekdudaegan Mountains(Hyangrobong~Guryongryeong) (최북단 백두대간(향로봉~구룡령) 능선부에 분포하는 관속식물상)

  • Oh, Hyunkyung;You, Juhan
    • Journal of Environmental Impact Assessment
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    • v.28 no.4
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    • pp.347-372
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    • 2019
  • The purpose of this study is to provide the basic data for management of the ecosystem of Baekdudaegan Mountains by surveying and analysing the vascular plants distributed from Hyangrobong area to Guryongryeong area. The numbers of vascular plants in the whole survey areas were summarized as 633 taxa including 95 families, 321 genera, 549 species, 4 subspecies, 70 varieties, and 10 forms. There were 285 taxa in Hyangrobong area, 256 taxa in Masanbong area, 318 taxa in Mt. Seoraksan area, 281 taxa in Mt. Jeombongsan area, 173 taxa in Bukamryeong and Ohsaekcheon valley area, 197 taxa in Soenadeuri area, 203 taxa in Yeongarigol area, and 168 taxa in Guryongryeong area. The endangered species were 2 taxa including Leontice microrrhyncha and Smilacina bicolor. The rare plants were 39 taxa including Pinus pumila, Halenia corniculata, Juniperus chinensis var. sargentii, and so forth. In IUCN Red List categories, there were 2 taxa of CR, 7 taxa of EN, 10 taxa of VU, 18 taxa of LC, and 2 taxa of DD. The Korean endemic plants were 23 taxa including Salix koriyanagi, Anemone koraiensis, Lespedeza maritima, Cirsium setidens, Saussurea seoulensis, Carex okamotoi, and so forth. The plants adaptable to climate change were 48 taxa including 17 taxa of endemic plants, 1 taxon of southern plant, and 30 taxa of northern plants. The naturalized plants were 32 taxa including Fallopia dumetorum, Cerastium glomeratum, Papaverrhoeas, Bidensfrondosa, Lolium perenne, and so forth. The invasive alien plants were 3 taxa including Rumex acetocella, Ambrosia artemisiifolia, and Ambrosia trifida.

Expression of Helicobacter pylori urease in plants to use as an edible vaccine

  • Gang, Gwi-Hyeon;Han, So-Cheon;Gang, Tae-Jin;Yang, Mun-Sik
    • 한국생물공학회:학술대회논문집
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    • 2003.10a
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    • pp.186-189
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    • 2003
  • Helicobacter pylori is the etiologic agent of human gastritis and peptic ulceration and produces urease as the major protein component on its surface. H. pylori urease is known to serve as a potent immunogen as well as major virulence factor. In order to express the recombinant urease in tobacco plants, a DNA fragment containing the minimal H. pylori urease gene cluster was subcloned into a plant expression vector. The recombinant vector was transformed to tobacco plants. The integration of the recombinant plasmids into tobacco chromosomal genome was verified by genomic PCR. Expression to mRNA was confirmed by Northern blot analysis, and expression to recombinant urease protein was observed by Western blot analysis. These results showed that the recombinant urease can be produced in tobacco plants and will be tested for immune response to use as an edible vaccine.

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Effect of ozone treatment on the expression of a foreign gene in transgenic poplar subclones (형질전환 포플라 subclone의 도입유전자 발현에 대한 오존처리의 영향)

  • 설일환;신동일
    • Journal of Life Science
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    • v.7 no.3
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    • pp.172-175
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    • 1997
  • Transgenic hybrid poplar subclones containing herbicide glyphosate resistant gene (aroA) were treated with ozone at the concentration of 100 nL L$^{-1}$ for 6 hr for 5 consecutive days. The foreign gene expression in leaves of all treated plants was reduced both at transcriptional and translational levels confirmed by Northern and Western blot analysis, respectively, as compared to non-treated control plants. These results indicated that the expression of foreign gene in transgenic plants could be affected by the environmental stresses. Thus, the performance of transgenic plants cultivated on field conditions may be lower than they are expected.

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Herbicide-resistant Transgenic Mongolian Bentgrass (Agrostis mongolica Roshev.) obtained by Agrobacterium-mediated Transformation

  • Vanjildorj, Enkhchimeg;Bae, Tae-Woong;Song, In-Ja;Kim, Kyung-Moon;Lim, Yong-Pyo;Lee, Hyo-Yeon
    • Korean Journal of Breeding Science
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    • v.40 no.2
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    • pp.128-135
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    • 2008
  • Herbicide resistance is the most common trait being tested and thus herbicide?resistant genetically modified plants are now the most widely cultivated worldwide. Here we developed herbicide?resistant transgenic Agrostis mongolica Roshev. by employing an efficient Agrobacterium?mediated transformation procedure with 25.2% of transformation efficiency. The identification and employment of regenerable and reproducible type of callus was one of the most critical factors to ensure success in this study. PCR analysis confirmed that the bar transgene was integrated into the genome of transgenic plants. The expression of 35S?bar gene was confirmed by Northern blot analysis. The transgenic plants showed complete resistance to herbicide, indicating that the bar gene is functional in transgenic plants.

The Ethnobotanical Investigation around National Parks in the Northern Area of Province Gyeongsangbuk-do, Korea

  • Lee, Ho-Young;Lee, Ji Yeon;Kang, Shin-Ho;Chung, Kyong-Sook
    • Korean Journal of Plant Resources
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    • v.30 no.6
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    • pp.623-639
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    • 2017
  • We investigated traditional knowledge about ethnobotanical uses around national parks in the northern areas of Province Gyeongsangbuk-do, with the focus on the Sobeaksan National Park area. Interviews were carried out to 138 residents at 42 places from 7 counties and/or cities, and verified species and usage information were categorized by taxonomic groups, usage, and used parts. The ethnobotanical species of the regions consisted of a total of 277 taxa: 244 species, 3 subspecies, 28 varieties, and 2 forma in 213 genera of 79 families. The significant native plants included one Endangered Species (Paeonia obovata Max.) and four endemic species (Salix hallaisanensis H. Lev., Aconitum pseudolaeve Nakai, Paulownia coreana Uyeki, and Cirsium setidens (Dunn) Nakai, Sasa coreana Nakai). About 17 Approved Species for Delivering Overseas designated by Korea Ministry of Environment have been also traditionally valuable in the regions. The main usage of the plants were edible (185 taxa) and medicinal (175 taxa). Leaves were most commonly used parts (109 taxa), followed by stems (73 taxa), fruits (69 taxa), roots (67 taxa), and wholes (57 taxa). The traditional usage of plants were diverse as those useful parts. Leaves, fruits and seeds were used as edible and medicinal; sprouts (young shoots) were mainly used as edible; but roots and flowers were used as medicinal. The study does not only provides examples of traditional uses of native plants, but also facilitates sustainable managements and developments of ethnobotanical knowledge for the contemporary society.

Effects on the Development of Plutella xylostella and Spodoptera litura after Feeding on Transgenic Cabbage Expressing Potato Proteinase Inhibitor II and Bar Genes

  • Lee, Yeon-Hee;Lee, Sang-Guei;Park, Beom-Seok;Lee, Young-Su;Jin, Yong-Moon;Kim, Ho-il;Suh, Seok-Cheol
    • Journal of Plant Biotechnology
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    • v.6 no.3
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    • pp.145-150
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    • 2004
  • Cabbage plants were transformed with the potato proteinase inhibitor II (PINII) gene, bar gene, and hpt gene using Agrobacterium. The expression of the PINII gene was driven by its own promoter which was wound-inducible. Ten transgenic plants were obtained from medium containing hygromycin as a selection antibiotic. The integration and expression of PINII and bar genes were confirmed by Southern and Northern hybridization. Growth and development of diamondback moths (Plutella xylostella) and tobacco cutworm (Spodoptera litura) larvae were examined on $T_1$ plants. The weight of the larvae and pupae of these two insects grown on transgenic plants was not different compared to those grown on wild type plants. However, the pupation and emergence rate of diamondback moths and tobacco cutworms fed on some transgenic plants was lower than on wild type plants. These results suggest that the PINII transgene under the control of a wound-induced promoter may be used for control of insects in transgenic cabbage through reduction of insect progeny number.

Transformation of Orchardgrass (Dactylis glomerata L.) with Glutathione Reductase Gene (Glutathione Reductase 유전자의 도입에 의한 오차드그래스의 형질전환)

  • 이효신;배은경;김기용;원성혜;정민섭;조진기
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.21 no.1
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    • pp.21-26
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    • 2001
  • To develop transgenic orchardgrass resistant to reactive oxygen species produced from environmental stresses, a vector with the cytosolic glutathione reductase cDNA (BcGRl) from Chinese cabbage was constructed under the control of the cauliflower mosaic virus 35S promoter and was introduced into orchardgrass using Agrobacterium tumefaciens EHA101. Transgenic plants from hygromycin-selected calli of orchardgrass did not show any morphological difference from wild-type plants. The results of PCR amplification and genomic Southern blot analysis confirmed the integration of foreign gene into the chromosome of transgenic orchardgrass. Northern blot analysis with total RNA from leaves also confirmed the constitutive expression of BcGR1 in transgenic orchardgrass.

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Molecular Coning of cDNA for Garlic Mosaic Virus Genome (마늘 모자이크 바이러스 게놈에 대한 cDNA의 클로닝)

  • 최연희
    • Journal of Plant Biology
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    • v.35 no.3
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    • pp.253-257
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    • 1992
  • Potyvirus group is the largest group among plant virus groups and damages severely plant hosts upon infectiQn. In order to investigate the mechanism by which potyviruses induce disease in plants, a cDNA clone 29-6 which is cOIlsidered to be a cDNA clone for garlic mosaic virus (GMV) was isolated. It did not hybridize to garlic latent virus genome, which is one of two major garlic viruses. Northern blot analysis shows that the genome size of garlic mosaic virus was about 9 kb. Clone 29-6 strongly hybridizes to poly(A) RNA isolated from garlic leaves, suggesting that GMV RNA is polyadenylated as other potyviruses. Nucleotide sequence analysis of cDNA clones overlapping with clone 29-6 showed that garlic plants are infected with various strains of garlic mosaic virus which are closely related to each other. other.

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Expression of Canavalia Iineata Leghemoglobin cDNA in Transgenic Nicotiana tabacum (형질전환된 담배에서 해녀콩 Leghemoglobin cDNA의 발현)

  • 이선영
    • Journal of Plant Biology
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    • v.38 no.2
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    • pp.203-209
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    • 1995
  • Tobacco (Nicotiana tahacum L. cv. Wisconsin 38) leaf discs were cocultivated with Agrohacterium carrying a leghemoglobin (Lb) cDNA from Canavalia lineata. Seven plants were regenerated from the transformed leaf discs on MS media supplemented with 0.5 mg/L BAP, 0.1 mg/L ${\alpha}-NAA$, 200 mg/L kanamycin and 500 mg/L carbenicillin. Southern hybridization and PCR of genomic DNA from transgenic plants showed that the Lb cDNA was stably integrated into the genome of the tobacco. Total RNA from the transgenic tobacco showed northern hybridization signal at 1,000 nt and PCR of the first strand cDNA synthesized from the total RNA amplified 0.5 kb Lb cDNA. Furthermore, western hybridization using a polyclonal antibody against soybean Lb showed a 15.8 kD LB-like band on SDS-PAGE of proteins from the transformed tobacco. These results demonstrated that the Lb cDNA of C. lineata was not only incorporated into the genome of tobacco, but also transcribed into mRNA and translated into Lb protein in the transformed tabacco.

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Overexpression of Ice Recrystallization Inhibition Protein (HvIRIP) from Barley Enhances Cold Tolerance in Transgenic rapeseed plants (HvIRIP 과발현 유채 형질전환체의 내한성 증진)

  • Roh, Kyung Hee;Park, Jong-Sug;Kang, Han-Chul;Kim, Jong-Bum;Jang, Young-Suk;Kim, Kwang-Soo;Yi, Hankuil
    • Journal of Applied Biological Chemistry
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    • v.58 no.4
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    • pp.325-332
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    • 2015
  • Rapeseed (Brassica napus) is now the second largest oilseed crop after soybean. Cold temperature tolerance is an important agronomic trait in winter rapeseed that determines the plant's ability to control below freezing temperatures. To improve cold tolerance of rapeseed plants, an expression vector containing an Barley Ice recrystallization inhibition protein (HvIRIP) cDNA driven by a cauliflower mosaic virus 35S promoter was transferred into rapeseed plants. Transgenic expression of HvIRIP was proved by southern- and northern-blot analyses. The level of freezing tolerance of transgenic $T_3$ plants was found to be significantly greater than that of wild-type rapeseed plants by freezing assay. Proline accumulation during cold stress was also highly induced in the transgenic rapeseed plants. The transgenic plants exhibited considerable tolerance against oxidative damage induced by cold stress. Our results indicated that heterologous HvIRIP expression in transgenic rapeseed plants may induce several oxidative-stress responsive genes to protect from cold stress.