Effect of ozone treatment on the expression of a foreign gene in transgenic poplar subclones

형질전환 포플라 subclone의 도입유전자 발현에 대한 오존처리의 영향

  • 설일환 (경북대학교 농업과학기술연구소) ;
  • 신동일 (대구효성카톨릭대학교 자연대학 식물육종학과)
  • Published : 1997.09.01

Abstract

Transgenic hybrid poplar subclones containing herbicide glyphosate resistant gene (aroA) were treated with ozone at the concentration of 100 nL L$^{-1}$ for 6 hr for 5 consecutive days. The foreign gene expression in leaves of all treated plants was reduced both at transcriptional and translational levels confirmed by Northern and Western blot analysis, respectively, as compared to non-treated control plants. These results indicated that the expression of foreign gene in transgenic plants could be affected by the environmental stresses. Thus, the performance of transgenic plants cultivated on field conditions may be lower than they are expected.

Keywords

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