• Proceedings of the Korean Society of Plant Biotechnology Conference
• /
• 2004.10a
• /
• pp.62-65
• /
• 2004

To protect the watermelon against soil-borne pathogens, we are currently producing disease-resistant transgenic root stock for the growth of watermelon, A defensin gene (J1-1) from Capsicum annum, a ACC deaminase gene from Pseudomonas syringae, a galactinol synthase (CsGolS) gene from Cucumis sativus, and a WRKY (CvWRKY2) gene from Citullus vulgaris were used as transgenes for disease resistance. The gene were transformed into a inbred line (6-2-2) of watermelon, Kong-dae watermelon and a inbred line (GO702S) of gourd, respectively, by Agrobacterium-mediated transformation. Putative transgenic plants were selected in medium containing 100mg/L kanamycin, and then integration of the genes into the genomic DNA were demonstrated by PCR analysis. Successful integration of the gene in regenerated plants was also confirmed by PCR (Figf 1), genomic Southern blot (Fig 2), RT-PCR (Fig 3), and Northern blot analysis(Fig 4). Several T1 lines having different transgene were produced, and disease resistance of the T1 lines are under estimation.

• Molecules and Cells
• /
• v.23 no.2
• /
• pp.207-214
• /
• 2007

lant ${\beta}$-1, 3-glucanases are involved in plant defense and in development. Very little data are available on the expression of rice glucanases both in developmental tissues and under various stresses. In this study, we cloned and characterized twenty-seven rice ${\beta}$-1, 3-glucanases (OsGlu) from at total of 71 putative glucanases. The OsGlu genes were obtained by PCR from a cDNA library and were classified into seven groups (Group I to VII) according to their DNA or amino acid sequence homology. Analysis of the expression of the twenty-seven OsGlu genes by Northern blotting revealed that they were differentially expressed in different developmental tissues as well as in response to plant hormones, biotic stress, high salt etc. OsGlu11 and 27 in Group IV were clearly expressed only in stem and leaf and were also induced strongly by SA (5 mM), ABA ($200{\mu}M$), and M. grisea. OsGlu1, 10, 11, and 14 were induced earlier and to higher levels in incompatible M. grisea interaction than in compatible one. Taken together, our findings suggest that the twenty-seven rice OsGlu gene products play diverse roles not only in plant defense but also in hormonal responses and in development.

• Plant Biotechnology Reports
• /
• v.2 no.1
• /
• pp.13-20
• /
• 2008

Potato (Solanum tuberosum L.), one of the most important food crops, is susceptible to a number of devastating fungal pathogens in addition to bacterial and other pathogens. Producing disease-resistant cultivars has been an effective and useful strategy to combat the attack of pathogens. Potato was transformed with Agrobacterium tumefaciens strain EHA101 harboring chitinase, (ChiC) isolated from Streptomyces griseus strain HUT 6037 and bialaphos resistance (bar) genes in a binary plasmid vector, pEKH1. Polymerase chain reaction (PCR) analysis revealed that the ChiC and bar genes are integrated into the genome of transgenic plants. Different insertion sites of the transgenes (one to six sites for ChiC and three to seven for bar) were indicated by Southern blot analysis of genomic DNA from the transgenic plants. Expression of the ChiC gene at the messenger RNA (mRNA) level was confirmed by Northern blot analysis and that of the bar gene by herbicide resistance assay. The results obviously confirmed that the ChiC and bar genes are successfully integrated and expressed into the genome, resulting in the production of bialaphos-resistant transgenic plants. Disease-resistance assay of the in vitro and greenhouse-grown transgenic plants demonstrated enhanced resistance against the fungal pathogen Alternaria solani (causal agent of early blight).

• Proceedings of the Plant Resources Society of Korea Conference
• /
• 2019.04a
• /
• pp.119-119
• /
• 2019

Pinus densiflora, the Korean Red Pine, is the predominant tree species of the cool, temperate forests of northeast Asia, occurring in pure stands across Korea, Japan, and parts of northern China and Russia. Pinus densiflora leaves, pollen, and bark have been widely used for traditional medicine, or edible purposes. However, pine cones contain many bioactive phytochemicals, but they are rarely used as natural raw materials. This study was conducted to evaluated the anti-inflammatory effect of pine cone extracts and its possbility of natural sources were evaluated. Pine cones were extracted with 80% methanol, concentrated and then partitioned with ethyl acetate, and the organic layer was used as a sample. The Pine cone Ethyl acetate Fraction (PEF) showed no toxicity to RAW 264.7 cells at a concentration of less than $50{\mu}g/ml$. PEF inhibited the production of nitric oxide (NO) in RAW 264.7 cells treated with lipopolysaccharide (LPS). Also, It suppressed the expression of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS) and transcription of nuclear factor-kappa B (NF-${\kappa}B$). These results suggest that pine cones can be used as an effective natural material for anti-inflammatory agent.

• Journal of Plant Biotechnology
• /
• v.30 no.2
• /
• pp.129-133
• /
• 2003

The factors for symptom development caused by Beat curly top virus(BCTV) have been analyzed by using a molecular genetic approach based on expressing BCTV encoded proteins in transgenic plants. BCTV open reading frame (ORF) L4 expression in transgenic Arabidopsis resulted in abnormal plant development and the production of callus inflorescence stems and bumpy trichomes, confiming that this gene alone is a primary symptom determinant. The L4 gene expression by northern hybridization in transgenic plants and a range of phenotypes were analyzed.

• Plant Resources
• /
• v.6 no.3
• /
• pp.165-169
• /
• 2003

American ginseng (Panax quinquefolium) is herbaceous perennial plants indigenous to North American forests. This is highly valued as medicinal herbs with a long history of collection from wild populations since 1716. Wild American ginseng distributed from Quebec in Canada to northern Florida in USA. A heavy concentration is found in the Appalachian mountains, although wild American ginseng is considered endangered. The price paid for field cultivated ginseng has dropped dramatically in the past 10 years, while the price for wild or woods cultivated ginseng has rised significantly. The price curve for ginseng resembles a roller coaster, reflecting not only supply and demand but many other factors. This information will be useful to understand American ginseng compared to Korean ginseng.

• Korean Journal of Plant Taxonomy
• /
• v.48 no.2
• /
• pp.129-133
• /
• 2018

Ceratopteris thalictroides is a semi-aquatic fern with a circumtropical distribution. Because this species is designated internationally on the IUCN Red List as requiring at least some concern, Korean populations are of great concern for the species' long-term survival, as they are at the northern limit of the species distribution. To establish an effective conservation strategy for those populations at the genetic level, we used the Mi-Seq platform to develop three sets of 25 polymorphic microsatellite markers for C. thalictroides, which is endangered in Korea. In populations sampled from Busan and Gochang, the number of alleles ranged from 2 to 13 (average of 5.64), and plants presented an expected heterozygosity of 0.000 to 0.860. These markers will be useful for evaluating the genetic status and conserving Korean populations of C. thalictroides more effectively.

• Journal of Korean Society of Forest Science
• /
• v.111 no.1
• /
• pp.1-18
• /
• 2022

In this study, the vascular plants of the Mt. Deokhangsan protected area were surveyed for the purpose of forest genetic resources conservation in Gangwon-do, South Korea. The flora on Mt. Deokhangsan were classified as follows 537 taxa, 90 families, 302 genera, 479 species, 4 subspecies, 51 varieties, and 3 forms. Among the taxa, 19 were Korean endemic plants and 17 were rare and endangered plants. Plants as indicators of limestone areas were found in 33 taxa. Naturalized plants were found in 27 taxa and species threatened by climate change were found in 33 taxa, including 11 endemic plant taxa and 22 northern plant taxa. Plants endemic to Korea and northern plants were mainly found at the study site, whereas southern plants were not found. Thus, Mt. Deokhangsan apparently has an environment suitable for the growth of northern plants.

• Korean Journal of Plant Taxonomy
• /
• v.43 no.4
• /
• pp.319-331
• /
• 2013

Records found on labels of specimens deposited at Kyoto University (KYO) and references about three Japanese taxonomists, Koidzumi, Gen'ichi (1883-1953), Ohwi, Jisaburo (1905-1977), Kitamura, Siro (1906-2002) were assembled to produce collector's itineraries from 1930 to 1935 in Korea. The quality of data on labels of the specimens varies, but most are only the collector's name and country of collection, often, the locality data are only textual, and the Chinese and Japanese names, as well as the ethnic dialects common to the region, varies widely. It is estimated that approximately 2,000 specimens collected from Korea by three taxonomists are currently held within the collections of Kyoto University herbarium (KYO). Koidzumi, who was the professor of Kyoto University, traversed different northern parts of the country, such as Island Jeju-do, Mt. Keumkang-san, Hamkyongbuk-do during summer (July to August) in 1932, 1933, and 1935. In 1930 and 1932, Ohwi spend three months in the unexplored mountains in northern parts, such as Hamkyeongnam-do, Hamkyeongbuk-do, and Gangwon-do. On the other hand, for two months in the middle of 1935 visited Jeju-do, Mt. Jirisan and travelled through southern parts. Unlike two previous botanists, major collections in Korea by Kitamura took place twice in one major area in northern part and Jeju-do and Mt. Keumgang-san in 1930, 1932, and 1935.

• Research in Plant Disease
• /
• v.14 no.3
• /
• pp.210-213
• /
• 2008

The occurrence of Rice stripe virus (RSV) has been confirmed in some parts of South Korea. In the current year, this was observed to be limited in the double cropping zones of the southern region. Earlier, RSV occurred largely in the west coast regions of the country, particularly in Gangwha, Gyeonggi-do in 2001; and Buan, Jeollabuk-do and Seocheon, Chungcheongnam-do in 2007. This study was carried out to determine the nationwide extent of RSV occurrence by investigating RSV infestations in different latitudes and altitudes. Result revealed a recent reported occurrence of RSV in the southern region (Milyang, latitude $35^{\circ}$), a reported occurrence in the northern region (Cheolwon, latitude $38^{\circ}$) in 2005, and in the middle northern region of the east coast (Goseon, latitude $38^{\circ}$) as newly confirmed from this investigation. Results also confirmed the occurrence of RSV in a wide range of altitude from the plains (Milyang, 17 m altitude) to the alpine regions (Jinbu, 576 m) including the middle mountains (Sangju, altitude 285 m). The RSV occurrence in Jinbu in the alpine region and at Goseong and most of the northern end regions were confirmed only this year. The results of the present study confirmed that RSV is a fast-spreading disease in rice plant cultivation regions of the entire country.