• Title/Summary/Keyword: normalization factor

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Identification of Endogenous Genes for Normalizing Titer Variation of Citrus Tristeza Virus in Aphids at Different Post-acquisition Feeding Times

  • Wang, Hongsu;Chen, Qi;Liu, Luqin;Zhou, Yan;Wang, Huanhuan;Li, Zhongan;Liu, Jinxiang
    • The Plant Pathology Journal
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    • v.38 no.4
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    • pp.287-295
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    • 2022
  • Citrus tristeza virus (CTV) is efficiently transmitted in a semi-persistent manner by the brown citrus aphid (Toxoptera citricida (Kirkaldy)). Currently, the most sensitive method for detecting plant viruses in insect vectors is reverse-transcription quantitative polymerase chain reaction (RT-qPCR). In this study, the elongation factor-1 alpha (EF-1α) gene and acidic p0 ribosomal protein (RPAP0) gene were confirmed to be suitable reference genes for RT-qPCR normalization in viruliferous T. citricida aphids using the geNorm, NormFinder, and BestKeeper tools. Then the relative CTV titer in aphids (T. citricida) at different post-acquisition feeding times on healthy plants was quantified by RT-qPCR using EF-1α and RPAP0 as reference genes. The relative CTV titer retained in the aphids gradually decreased with increasing feeding time. During the first 0.5 h of feeding time on healthy plants, the remaining CTV titer in aphids showed about 80% rapid loss for the highly transmissible isolate CT11A and 40% loss for the poorly transmissible isolate CTLJ. The relative CTV titer in aphids during more than 12 h post-acquisition times for CT11A was significantly lower than at the other feeding times, which is similar to the trend found for CTLJ. To our knowledge, this is the first report about the relative titer variation of CTV remaining in T. citricida at different post-acquisition feeding times on healthy plants.

Dislocation in Semi-infinite Half Plane Subject to Adhesive Complete Contact with Square Wedge: Part I - Derivation of Corrective Functions (직각 쐐기와 응착접촉 하는 반무한 평판 내 전위: 제1부 - 보정 함수 유도)

  • Kim, Hyung-Kyu
    • Tribology and Lubricants
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    • v.38 no.3
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    • pp.73-83
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    • 2022
  • This paper is concerned with an analysis of a surface edge crack emanated from a sharp contact edge. For a geometrical model, a square wedge is in contact with a half plane whose materials are identical, and a surface perpendicular crack initiated from the contact edge exists in the half plane. To analyze this crack problem, it is necessary to evaluate the stress field on the crack line which are induced by the contact tractions and pseudo-dislocations that simulate the crack, using the Bueckner principle. In this Part I, the stress filed in the half plane due to the contact is re-summarized using an asymptotic analysis method, which has been published before by the author. Further focus is given to the stress field in the half plane due to a pseudo-edge dislocation, which will provide a stress solution due to a crack (i.e. a continuous distribution of edge dislocations) later, using the Burgers vector. Essential result of the present work is the corrective functions which modify the stress field of an infinite domain to apply for the present one which has free surfaces, and thus the infiniteness is no longer preserved. Numerical methods and coordinate normalization are used, which was developed for an edge crack problem, using the Gauss-Jacobi integration formula. The convergence of the corrective functions are investigated here. Features of the corrective functions and their application to a crack problem will be given in Part II.

Interactions between the National Assembly and the Executive in Korea: With Emphasis on Legislative, Budgetary and Investigatory Powers (국회와 정부의 관계: 입법권, 재정권, 국정조사권을 중심으로)

  • Park, Jai-Chang
    • Korean Journal of Legislative Studies
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    • v.15 no.1
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    • pp.39-63
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    • 2009
  • Retrospecting the past 60 years of interactions between the executive and the legislature in Korea, it is found that the Korean National Assembly is neither a minimal legislature nor a marginal legislature any more. The symbolic role of the Assembly has acquired a remarkable growth and profound strength and there is no need to worry about institutional viability any further. However, its role as a resolver or manager of social conflicts is still under the dominance of the executive and shows even an indication that it might be worsened. Such incapability of the legislature owed much to the vertical control from the outside especially during the authoritarian regimes, however, nowadays it owes much to the inability of consensus building among the out-bursting controversial and horizontal opinions inside of it. Not sufficient managerial capability required for exercising autonomous decision making power efficiently is a major detrimental factor. It traces back to the inability of party politics and demassifying political leadership. Normalization of party politics is identified as one of the essential prerequisites for Korean political development in this regard.

An Improvement of Stochastic Feature Extraction for Robust Speech Recognition (강인한 음성인식을 위한 통계적 특징벡터 추출방법의 개선)

  • 김회린;고진석
    • The Journal of the Acoustical Society of Korea
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    • v.23 no.2
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    • pp.180-186
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    • 2004
  • The presence of noise in speech signals degrades the performance of recognition systems in which there are mismatches between the training and test environments. To make a speech recognizer robust, it is necessary to compensate these mismatches. In this paper, we studied about an improvement of stochastic feature extraction based on band-SNR for robust speech recognition. At first, we proposed a modified version of the multi-band spectral subtraction (MSS) method which adjusts the subtraction level of noise spectrum according to band-SNR. In the proposed method referred as M-MSS, a noise normalization factor was newly introduced to finely control the over-estimation factor depending on the band-SNR. Also, we modified the architecture of the stochastic feature extraction (SFE) method. We could get a better performance when the spectral subtraction was applied in the power spectrum domain than in the mel-scale domain. This method is denoted as M-SFE. Last, we applied the M-MSS method to the modified stochastic feature extraction structure, which is denoted as the MMSS-MSFE method. The proposed methods were evaluated on isolated word recognition under various noise environments. The average error rates of the M-MSS, M-SFE, and MMSS-MSFE methods over the ordinary spectral subtraction (SS) method were reduced by 18.6%, 15.1%, and 33.9%, respectively. From these results, we can conclude that the proposed methods provide good candidates for robust feature extraction in the noisy speech recognition.

Dietary Effect of Silk Protein Sericin or Fibroin on Plasma and Epidermal Amino Acid Concentration of NC/Nga Mice (실크 단백질 Sericin 및 Fibroin의 식이 공급이 아토피 피부염 동물 모델 NC/Nga Mice의 혈장과 표피의 유리 아미노산 함량에 미치는 영향)

  • Kim, Hyun-Ae;Park, Kyung-Ho;Yeo, Joo-Hong;Lee, Kwang-Gili;Jeong, Do-Hyeon;Kim, Sung-Han;Cho, Yun-Hi
    • Journal of Nutrition and Health
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    • v.39 no.6
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    • pp.520-528
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    • 2006
  • Free amino acids in epidermis function as a major component of Natural Moisturizing Factor (NMF), which maintains the optimal level of water in skin even at the low humidity. In fact, the depletion of free amino acids is reported in the epidermis of atopic dermatitis, the skin condition involving dryness. As an effort searching the dietary source for improving the level of water and free amino acid in epidermis, the dietary effects of silk protein, sericin (S) and fibroin (F) on trans epidermal water loss (TEWL), and plasma and epidermal levels of free amino acids were compared in this study. Thirty of male NC/Nga mice, an animal model of atopic dermatitis, were divided into three groups: group CA as an atopic control with control diet, group S: 1% sericin diet and group F: 1% fibroin diet. Ten of male BALB/c mice were served as group C (control group) with control diet. All mice were fed on diet and water ad libitum for 10weeks. Dry skin condition was established in group CA as TEWL was increased (148.7% of group C). In parallel, epidermal level of glutamate, one of major amino acids functioning as NMF, was dramatically decreased and epidermal levels of methionine and alanine were inversely elevated. Dietary supplementation of sericin (group S) reduced TEWL at the similar level with group C and increased epidermal levels of glutamate as well as serine and glycine, the other major amino acids as NMF. Despite a marked decrease of methionine and alanine, the reduction of TEWL and epidermal levels of glutamate, serine and glycine of group F were less than of group S. Furthermore, in contrast to similar levels of other free amino acids in plasma and epidermis of group S and group C, plasma and epidermal levels of other free amino acids, specifically phenylalanine, isoleucine, cysteine and tyrosine in epidermis of group F, were significantly higher than of group C. Together, our data demonstrate that dietary supplementation of sericin is more effective at improving dry skin condition that paralleled with the normalization of free amino acids in plasma and epidermis of NC/Nga mice.

An Analysis on the Investment Determinants for Insolvent Housing Development Projects (건설회사의 공동주택 PF 부실사업장에 대한 투자결정요인 분석)

  • An, Kukjin;Cho, Yongkyung;Lee, Sangyoub
    • Korean Journal of Construction Engineering and Management
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    • v.15 no.2
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    • pp.112-121
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    • 2014
  • After IMF bailout crisis in Korea, project financing has been employed as a major funding vehicle for the housing development. In 2008, the recession of housing market due to the global financial crisis had an significant impact on the increasing insolvent site of PF based housing development project, resulting in serious impact to whole economy as a chain effect. In order to resolve this vicious circle of bankruptcy, the major construction companies were urged to take over the insolvent sites and invest to them for normal project exit, and finally play a critical role in normalization of market. Therefore, this study aims to define the core factors for decision making to invest to insolvent site and find out differences among constructors, developers, financial lenders. The results from AHP analysis, the profitability was the most important factor to constructors. Moreover, even though the location merit is little less, through competitive price, we can assure that stable profitability is most important factor to decide to invest in insolvent site. In conclusion, the price is cheap, is highly feasible, if the land secured, major construction company will participate in a PF business investment. These findings were verified by the investment case of major construction company.

Establishing meteorological drought severity considering the level of emergency water supply (비상급수의 규모를 고려한 기상학적 가뭄 강도 수립)

  • Lee, Seungmin;Wang, Wonjoon;Kim, Donghyun;Han, Heechan;Kim, Soojun;Kim, Hung Soo
    • Journal of Korea Water Resources Association
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    • v.56 no.10
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    • pp.619-629
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    • 2023
  • Recent intensification of climate change has led to an increase in damages caused by droughts. Currently, in Korea, the Standardized Precipitation Index (SPI) is used as a criterion to classify the intensity of droughts. Based on the accumulated precipitation over the past six months (SPI-6), meteorological drought intensities are classified into four categories: concern, caution, alert, and severe. However, there is a limitation in classifying drought intensity solely based on precipitation. To overcome the limitations of the meteorological drought warning criteria based on SPI, this study collected emergency water supply damage data from the National Drought Information Portal (NDIP) to classify drought intensity. Factors of SPI, such as precipitation, and factors used to calculate evapotranspiration, such as temperature and humidity, were indexed using min-max normalization. Coefficients for each factor were determined based on the Genetic Algorithm (GA). The drought intensity based on emergency water supply was used as the dependent variable, and the coefficients of each meteorological factor determined by GA were used as coefficients to derive a new Drought Severity Classification Index (DSCI). After deriving the DSCI, cumulative distribution functions were used to present intensity stage classification boundaries. It is anticipated that using the proposed DSCI in this study will allow for more accurate drought intensity classification than the traditional SPI, supporting decision-making for disaster management personnel.

The Site Effect of the Broadband Seismic Stations in Korea (국내 광대역 지진 관측소의 부지효과)

  • Wee, Soung-Hoon;Kim, Sung-Kyun
    • Economic and Environmental Geology
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    • v.41 no.2
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    • pp.225-242
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    • 2008
  • The site effect for 23 broadband seismic stations in the southern Korean Peninsula was estimated by using the spectral ratio of coda waves. In principle, the site effect means the pure amplification below the station excluding effects of seismic source and attenuation in the wave transmission. However, the site effect determined in this study is equivalent with the relative site amplification factor to the mean amplification for all stations. A total of 500 three-component seismograms from 35 earthquakes, of which magnitude ranged from 2.5 to 5.1 occurred from January, 2001 to January, 2007 was used to obtain the site amplification factor. The site amplification factors were estimated for the frequency bands centered at 0.2, 0.5, 1, 2, 5, 10, 15, and 20 Hz. It was found that the factors for two horizontal components of transverse and radial records were concordant with each other in the all frequency bands. However, the factor for the vertical component was found to be systematically lower than those for two horizontal components. The factors obtained in the low frequency band below 2 Hz ranged from 0.5 to 1.5 in all seismic stations except for KMA and KIGAM stations in Bagryeongdo (BRD1 and BRD2) of which factor showed high value above 1.5. Some stations such as SEO, SNU, HKU, NPR, and GKPI showed high value above 1.5 in the high frequency band from 5 to 20 Hz. Especially, the factors of GKP1 station represented extremely high value ranging from 1.8 to 7.8. Also, the factors for stations of KWJ, SND, and ULJ showed low value below 0.5. The spatial distribution for the relative amplification factor represented a tendency of being approximately lower in north-eastern area than south-western area in the southern Korean Peninsula.

Prognostic Factors after Arthroscopic Treatment of Infectious Knee Arthritis (감염성 슬관절염의 관절경적 치료 이후 예후 인자에 대한 분석)

  • Kang, Sang-Woo;Choi, Eui-Sung;Kim, Dong-Soo;Jung, Ho-Seung;Hong, Seok-Hyun;Go, Ban-Suk
    • Journal of the Korean Orthopaedic Association
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    • v.54 no.1
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    • pp.30-36
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    • 2019
  • Purpose: This study examined the effects of gender, age, underlying disease, duration after onset of symptoms, preoperative invasive procedures, bacterial culture of joint fluid, and stage of infection by the Gachter classification on the prognosis of patients with infectious knee arthritis who underwent arthroscopic surgery. Materials and Methods: From June 2014 to December 2016, 51 patients who underwent arthroscopic surgery for infective knee arthritis were enrolled in this study. The average follow-up period was 14.2±2.1 months (range, 12-20 months). The subjects were 27 men (52.9%) and 24 women (47.1%), with an average age of 55.1±17.6 years (range, 13-84 years). A preoperative evaluation of the joint aspiration with a count of more than 50,000 leukocytes and a polymorphonuclear leukocyte count of 95% or more was performed. All patients underwent arthroscopic surgery and postoperative continuous joint irrigation. Results: The initial mean value of the C-reactive protein decreased from 9.55±6.76 mg/dl (range, 1.51-31.06 mg/dl) to a final mean of 0.74±1.26 mg/dl (range, 0.08-6.77 mg/dl); the mean duration of C-reactive protein normalization was 27.6±18.9 days (range, 8-93 days). Among the 51 patients who received arthroscopic surgery and antibiotics, 44 patients (86.3%) with infectious knee arthritis completed treatment with improved clinical symptoms, such as fever, pain, and edema, and the C-reactive protein decreased to less than 0.5 mg/dl. Finally, 5 cases were treated with two or more arthroscopic operations, and 2 cases were converted to arthroplasty after prosthesis of antibiotic-loaded acrylic cement. Conclusion: The duration of surgery after the onset of symptoms and the stage according to the Gächter classification are important prognostic factors for predicting the successful treatment of infectious knee arthritis. On the other hand, the other factors were not statistically significant. Nevertheless, patients with bacteria cultured from the joint fluids appear to reflect the treatment period because the period of normalization of the C-reactive protein is shorter than that of the control group.

Radiation Absorbed Dose Calculation Using Planar Images after Ho-166-CHICO Therapy (Ho-166-CHICO 치료 후 평면 영상을 이용한 방사선 흡수선량의 계산)

  • 조철우;박찬희;원재환;왕희정;김영미;박경배;이병기
    • Progress in Medical Physics
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    • v.9 no.3
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    • pp.155-162
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    • 1998
  • Ho-l66 was produced by neutron reaction in a reactor at the Korea Atomic Energy Institute (Taejon, Korea). Ho-l66 emits a high energy beta particles with a maximum energy of 1.85 MeV and small proportion of gamma rays (80 keV). Therefore, the radiation absorbed dose estimation could be based on the in-vivo quantification of the activity in tumors from the gamma camera images. Approximately 1 mCi of Ho-l66 in solution was mixed into the flood phantom and planar scintigraphic images were acquired with and without patient interposed between the phantom and scintillation camera. Transmission factor over an area of interest was calculated from the ratio of counts in selected regions of the two images described above. A dual-head gamma camera(Multispect2, Siemens, Hoffman Estates, IL, USA) equipped with medium energy collimators was utilized for imaging(80 keV${\pm}$10%). Fifty-nine year old female patient with hepatoma was enrolled into the therapeutic protocol after the informed consent obtained. Thirty millicuries(110MBq) of Ho-166-CHICO was injected into the right hepatic arterial branch supplying hepatoma. When the injection was completed, anterior and posterior scintigraphic views of the chest and pelvic regions were obtained for 3 successive days. Regions of interest (ROIs) were drawn over the organs in both the anterior and posterior views. The activity in those ROIs was estimated from geometric mean, calibration factor and transmission factors. Absorbed dose was calculated using the Marinelli formula and Medical Internal Radiation Dose (MIRD) schema. Tumor dose of the patient treated with 1110 MBq(30 mCi) Ho-l66 was calculated to be 179.7 Gy. Dose distribution to normal liver, spleen, lung and bone was 9.1, 10.3, 3.9, 5.0 % of the tumor dose respectively. In conclusion, tumor dose and absorbed dose to surrounding structures were calculated by daily external imaging after the Ho-l66 therapy for hepatoma. In order to limit the thresholding dose to each surrounding organ, absorbed dose calculation provides useful information.

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