• Journal of Microbiology and Biotechnology
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• 제25권2호
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• pp.206-216
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• 2015

To clarify the role of surface protective antigen A (SpaA) in the pathogenesis of Erysipelothrix rhusiopathiae C43065 (serotype 2), the spaA deletion mutant of E. rhusiopathiae ${\Delta}spaA$ was constructed by homologous recombination. The virulence of the ${\Delta}spaA$ mutant decreased more than 76-fold compared with that of the wild-type strain C43065 in mice. The mutant strain was sensitive to the bactericidal action of swine serum, whereas the wild-type strain was resistant. The adhesion of wild-type strain to MEF cells was inhibited significantly by treatment with rabbit antiserum against recombinant SpaA (rSpaA) as compared with the treatment with normal rabbit serum, but the mutant strain was not affected. The mutant strain was readily taken up by mouse peritoneal macrophages in the normal rabbit serum, whereas the wild-type strain was resistant. Whereas the rabbit antiserum against rSpaA promoted the phagocytosis of wild-type strain by macrophages, the mutant strain was not affected. In addition, mice vaccinated with the formalin-killed mutant strain were provided 40% protection against challenge by the homologous virulent strain as compared with those with wild-type strain, NaOH-extracted antigen, or rSpaA, which provided more than 80% protection against the same infection. These suggested that SpaA has an important role in the pathogenesis of E. rhusiopathiae infection and could be a target for vaccination against swine erysipelas.

• 대한조선학회논문집
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• 제51권6호
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• pp.489-494
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• 2014

Estimation of correct ice load under various operating conditions is important during the design and the operation stages of an icebreaker. Normal operating conditions are expected from the official field ice trials and also from general ice transit action. In this paper ice load for the Korean icebreaking research vessel, ARAON, under normal operating condition, is discussed. Published ice load data from full-scale sea trials of six icebreakers were analysed to derive an empirical ice load prediction formula. The IBRV ARAON had sea ice trials during 2010 and 2012 summer season. Strain gauge signal were recorded during her icebreaking voyage and the measured strain data were converted to the equivalent hull stress values. The effect of ARAON's speed in ice and the hull stresses are investigated. By comparing the empirical formula and ice load calculation based von measured data, it is recommended to use the empirical ice load estimation formula for the initial design stage.

• 한국윤활학회:학술대회논문집
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• 한국윤활학회 2004년도 학술대회지
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• pp.245-248
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• 2004

The press cutter is productive equipment that practically manufactures materials such as fabrics, papers, films, leathers, rubbers etc. into the desired shapes using cutting method. Plate cutting process is one of the primary energy absorbing mechanisms in a grounding or collision event. The cutting mechanism is complicated and involves plastic flow of plate in the vicinity of the tip, friction between wedge and plate, deformation of plate. In this paper, we studied the effect of friction between cutter and plastic sheet for producing precise and superior products. The press cutter is analyzed numerically using MARC finite element program according to the variation of friction coefficients. The FEM results showed that normal stress, equivalent cauchy stress, normal total strain, equivalent total strain are good when friction coefficient is 0.0 and shear stress, shear total strain are good when friction coefficient is 0.8.

• Journal of Mechanical Science and Technology
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• 제14권1호
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• pp.93-102
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• 2000

The objective of the present study is to investigate the pressure-strain correlation terms of the Reynolds stress models for the three dimensional turbulent boundary layer in a $30^{\circ}$ bend tunnel. The numerical results obtained by models of Launder, Reece and Rodi (LRR) , Fu and Speziale, Sarkar and Gatski (SSG) for the pressure-strain correlation terms are compared against experimental data and the calculated results from the standard k-${\varepsilon}$ model. The governing equations are discretized by the finite volume method and SIMPLE algorithm is used to calculate the pressure field. The results show that the models of LRR and SSG predict the anisotropy of turbulent structure better than the standard k-${\varepsilon}$ model. Also, the results obtained from the LRR and SSG models are in better agreement with the experimental data than those of the Fu and standard k-${\varepsilon}$ models with regard to turbulent normal stresses. Nevertheless, LRR and SSG models do not effectively predict pressure-strain redistribution terms in the inner layer because the pressure-strain terms are based on the locally homogeneous approximation. Therefore, to give better predictions of the pressure-strain terms, non-local effects should be considered.

• 지구물리와물리탐사
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• 제7권1호
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• pp.25-32
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• 2004

We have carried out laboratory measurements of P-wave velocity and deformation strain during $CO_2$ injection into a porous sandstone sample, in dry and water-saturated conditions. The rock sample was cylindrical, with the axis normal to the bedding plane, and fluid injection was performed from one end. Using a piezoelectric transducer array system, we mapped fluid movement during injection of distilled water into dry sandstone, and of gaseous, liquid, and supercritical $CO_2$ into a water-saturated sample. The velocity changes caused by water injection ranged from $5.61\;to\;7.52\%$. The velocity changes caused by $CO_2$ injection are typically about $-6\%$, and about $-10\%$ for injection of supercritical $CO_2$, Such changes in velocity show that the seismic method may be useful in mapping $CO_2$ movement in the subsurface. Strain normal to the bedding plane was greater than strain parallel to the bedding plane during $CO_2$ injection; injection of supercritical $CO_2$ showed a particularly strong effect. Strain changes suggest the possibility of monitoring rock mass deformation by using borehole tiltmeters at geological sequestration sites. We also found differences associated with $CO_2$ phases in velocity and strain changes during injection.

• 한국구조물진단유지관리공학회 논문집
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• 제4권2호
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• pp.103-111
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• 2000

The objectives of the study are to know the strain distribution and modal dynamic behaviour of steel bridge girders by actual traffic load. The live load effect depends on many parameters including the span length, gross vehicle weight, axle weight, axle configuration so on. For the estimation of static and dynamic characteristic, strain data caused by moving loads and traffic characteristics of passing vehicle under actual traffic load have measured using Bridge Weigh in Motion. To confirm the reliability of BWIM system, strain data measured using the $120{\Omega}$ strain gauge under the same condition. It is considered that the data acquired from BWIM system have reliability through the analysis and comparison between stress measured by strain data from BWIM and computed by FEM. Additionally according to the measured strain data of up-line and down-line on the highway, the up-line bridge grows more faster than the down-line bridge and girder 4 and 5 carry more load when vehicles pass the inner line and girder 2 and 3 does when vehicles pass the outer line as this case(the bridge composed with 5 girders).

• 대한기계학회논문집
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• 제15권1호
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• pp.169-178
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• 1991

본 연구에서는 평면 변형률 강소성 유한요소법을 이용하여 유한요소 수식화를 유도하고 금형이 해석저인 함수로 묘사되는 드로잉 공정을 해석하고, 금형이 해석적인 함수로 묘사되지 않는 실제적인 자동차 성형품의 드로잉 공정을 해석하여 기존의 결과 와 비교하여 본 방법의 타당성을 검토하였다.

• Structural Engineering and Mechanics
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• 제47권2호
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• pp.185-207
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• 2013

In flexural strength design of normal-strength concrete (NSC) beams, it is commonly accepted that the distribution of concrete stress within the compression zone can be reasonably represented by an equivalent rectangular stress block. The stress block it governed by two parameters, which are normally denoted by ${\alpha}$ and ${\beta}$ to stipulate the width and depth of the stress block. Currently in most of the reinforced concrete (RC) design codes, ${\alpha}$ and ${\beta}$ are usually taken as 0.85 and 0.80 respectively for NSC. Nonetheless, in an experimental study conducted earlier by the authors on NSC columns, it was found that ${\alpha}$ increases significantly with strain gradient, which means that larger concrete stress can be developed in flexure. Consequently, less tension steel will be required for a given design flexural strength, which improves the ductility performance. In this study, the authors' previously proposed strain-gradient-dependent concrete stress block will be adopted to produce a series of design charts showing the maximum design limits of flexural strength and ductility of singly-and doubly-NSC beams. Through the design charts, it can be verified that the consideration of strain gradient effect can improve significantly the flexural strength and ductility design limits of NSC beams.

• 대한기계학회논문집A
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• 제32권10호
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• pp.844-849
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• 2008

We present a novel cell deformability monitoring chip based on the digitally measured cell lysis rate which is dependent on the areal strain of the cell membrane. This method offers simple cell deformability monitoring by automated high-throughput testing system. We suggest the filter design considering the areal strain imposed on the cell membrane passing through the filter array having gradually increased orifice length. In the experiment using erythrocytes, we characterized the cell deformability in terms of average fracture areal strain which was $0.24{\pm}0.014\;and\;0.21{\pm}0.002$ for normal and chemically treated erythrocytes, respectively. We also verified that the areal strain of 0.15 effectively discriminates the deformability difference of normal and chemically treated erythrocytes, which can be applied to the clinical situation. We compared the lysis rates and their difference for the samples from different donors and found that the present chips can be commonly used without any calibration process. The experimental results demonstrate the simple structure and high performance of the present cell deformability monitoring chips, applicable to simple and cost-effective cell aging process monitoring.

• 한국지반신소재학회논문집
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• 제2권1호
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• pp.57-68
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• 2003

Strain-softening model is developed to characterize the interface behavior of geomembrane with geotextile and geosynthetic clay liner(GCL). The model proposed in this research is calibrated by using data from direct shear tests conducted on smooth and textured geomembrane. The research is divided into two regions, pre-peak and post-peak, to take into account of strain-softening effect. Although slight difference between measured and back calculated data is observed under high normal stress, good agreements, in general, are found from back calculations. Especially, good consistency is observed in the case of low normal stress. Based on the results, it can be concluded that the proposed model can be a reasonable constitutive law to figure out the behavior of strain-softening between interfaces of geomembrane. In addition, DSC(Disturbed State Concept) model is also presented for further application in geosynthetic interfaces.