• Bulletin of the Korean Chemical Society
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• 제25권9호
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• pp.1366-1370
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• 2004

Controlled pore glass bead was modified with bovine serum albumin (BSA), and glutathione (GSH) was immobilized through three kinds of linkers on top of BSA. Bis(3-sulfo-N-hydroxysuccinimide suberate) sodium salt $(BS^3)$, N-hydroxysuccinimide 3-(2-pyridyldithio)propionate (SPDP), or N-hydroxysuccinimide 4-maleimidobutyrate (GMBS) was introduced into the BSA-bound matrix. Subsequently, GSH was immobilized by addition of thiol side chain into the maleimido moiety, replacing a disulfide group, or formation of an amide group upon releasing 3-sulfo-N-hydroxysuccimide group. It was observed that conjugation methodology played a critical role for activity of the immobilized GSH. SDS-PAGE chromatogram showed that the matrix of glutathione immobilized on BSA through GMBS manifested high selectivity towards glutathione-S-transferase (GST) in cell lysate.

• 대한전기학회:학술대회논문집
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• 대한전기학회 2007년도 제38회 하계학술대회
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• pp.1524-1525
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• 2007

A flexible tactile sensor module based on polyimide matrix integrated with sensing elements and pluggable terminals connector was fabricated by polymer micromachining technology for robotic applications. The tactile sensor arrays are composed of $4{\times}4$, $8{\times}8$ and $16{\times}16$ sensing elements connected with pluggable terminals connector, respectively. Especially, both the tactile sensor array and the pluggable terminals are formed in the sensor module during the fabrication process. The fabricated tactile sensor module is measured continuously in the normal force range of $0{\sim}1N$ with tactile sensor auto-evaluation system. The value of resistance is relatively increased linearly with normal force in the overall range. The variation rate of resistance is about 2.0%/N in the range of $0{\sim}0.6N$ and 1.5%/N in the range of $0.6{\sim}1N$. Also, the flexibility of the sensing module is adequate to be placed on any curved surface as cylinder because the matrix consists of polymer and metal thin film.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2005년도 추계학술대회 논문집
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• pp.248-251
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• 2005

To investigate the degeneration process in the intervertebral disc, a three dimensional (3D) poroelastic finite-element (FE) model was developed. Disc was modeled as two different regions, such as annulus modeled with fiber reinforced 20 node poroelastic ground matrix and nucleus having large porosity. Excess Von Mises stress in the disc element assumed to be a possible source of degeneration under compressive loading condition. Recursive calculation was continued until the desired convergence was attained by changing the permeability and porosity of those elements, which could be predicted from the previous iteration. The degenerated disc model showed that relatively small compressive stresses were generated in the nucleus elements compared to normal disc. Its distribution along the sagittal plane was matched well with a previously reported experimental result. Contrasts to this result, pore pressures in the nucleus were higher than those in the normal disc. Total stress indicated similar values for two different models. This new approach using poroelastic modeling could provide the explanation of the interaction between fluid and solid matrix in the disc during the degeneration process.

• Journal of the Korean Statistical Society
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• 제24권1호
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• pp.257-266
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• 1995

Consider a p-variate $(p \geq 4)$ normal distribution with mean $\b{\theta}$ and identity covariance matrix. For estimating $\b{\theta}$ under a quadratic loss we investigate the behavior of risks of Stein-type estimators which shrink the usual estimator toward the mean of observations. By using concavity of the function appearing in the shrinkage factor together with new expectation identities for noncentral chi-squared random variables, a characterization of estimators with nondecreasing risk is obtained.

• 한국농공학회지
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• 제43권2호
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• pp.132-139
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• 2001

This study deals with coal-mine waste (CMW) for use in concrete as a replacement of normal aggregates. The CMW was collected from Sabuk region. Ganwon-do. Fine and coarse aggregates from CMW were prepared by using a crusher and separating debris with #4 sieve. CMW aggregates showed good physical and mechanical properties with having specific gravity over 2.65, absorption less than 1%, and abrasion ratio below 20%. However, particle shape of CMW was poor because of non-isotropic nature of matrix which cause particles to be long or flat. Since irregular particles caused a poor workability, to make workability better, a 1/4 of coarse aggregate was replaced with normal aggregate together with a superplasticizer. Compressive strength and other mechanical properties of CMW concrete were very good. Color of the concrete was darker than normal concrete due to black color of CMW. In conclusion, characteristics of CMW concrete was acceptable for use as a structural concrete material.

• 대한수학회논문집
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• 제34권1호
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• pp.127-136
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• 2019

This article concerns the normal property of elements on Jacobson and nil radicals which are generalizations of commutativity. A ring is said to be right njr if it satisfies the normal property on the Jacobson radical. Similarly a ring is said to be right nunr (resp., right nlnr) if it satisfies the normal property on the upper (resp., lower) nilradical. We investigate the relations between right duo property and the normality on Jacobson (nil) radicals. Related examples are investigated in the procedure of studying the structures of right njr, nunr, and nlnr rings.

• 한국지구과학회지
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• 제38권3호
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• pp.173-181
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• 2017

The stability of the steady Rossby-Haurwitz wave (R-H wave) in the nondivergent barotropic model (NBM) on the sphere was investigated with the normal mode method. The linearized NBM equation with respect to the R-H wave was formulated into the eigenvalue-eigenvector problem consisting of the huge sparse matrix by expanding the variables with the spherical harmonic functions. It was shown that the definite threshold R-H wave amplitude for instability could be obtained by the normal mode method. It was revealed that some unstable modes were stationary, which tend to amplify without the time change of the spatial structure. The maximum growth rate of the most unstable mode turned out to be in almost linear proportion to the R-H wave amplitude. As a whole, the growth rate of the unstable mode was found to increase with the zonal- and total-wavenumber. The most unstable mode turned out to consist of more-than-one zonal wavenumber, and in some cases, the mode exhibited a discontinuity over the local domain of weak or vanishing flow. The normal mode method developed here could be readily extended to the basic state comprised of multiple zonalwavenumber components as far as the same total wavenumber is given.

• BMB Reports
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• 제40권1호
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• pp.88-94
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• 2007

Matrix metalloproteinase-9 (MMP-9) plays a pivotal role in the turnover of extracellular matrix (ECM) and in the migration of normal and tumor cells in response to normal physiologic and numerous pathologic conditions. Here, we show that the transcription of the MMP-9 gene is induced by lipopolysaccharide (LPS) stimulation in cells of a macrophage lineage (RAW 264.7 cells). We provide evidence that the NF-$\kappa$B binding site of the MMP-9 gene contributes to its expression in the LPS-signaling pathway, since mutation of NF-$\kappa$B binding site of MMP-9 promoter leads to a dramatic reduction in MMP-9 promoter activation. In addition, the degradation of l$\kappa$B$\alpha$;, and the presences of myeloid differentiation protein (MyD88) and tumor necrosis factor receptor-associated kinase 6 (TRAF6) were found to be required for LPS-activated MMP-9 expression. Chromatin immunoprecipitation (ChIP) assays showed that functional interaction between NF-$\kappa$B and the MMP-9 promoter element is necessary for LPS-activated MMP-9 induction in RAW 264.7 cells. In conclusion, our observations demonstrate that NF-$\kappa$B contributes to LPS-induced MMP-9 gene expression in a mouse macrophage cell line.

• Structural Engineering and Mechanics
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• 제58권1호
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• pp.59-91
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• 2016

Laminated composite shells are commonly used in various engineering applications including aerospace and marine structures. In this paper, using semi-analytical finite strip method, the buckling behavior of laminated composite deep as well as thick shells of revolution under follower forces which remain normal to the shell is investigated. The stiffness caused by pressure is calculated for the follower forces subjected to external fibers in thick shells. The shell is divided into several closed strips with alignment of their nodal lines in the circumferential direction. The governing equations are derived based on first-order shear deformation theory which accounts for through thickness-shear flexibility. Displacements and rotations in the middle surface of shell are approximated by combining polynomial functions in the meridional direction as well as truncated Fourier series with an appropriate number of harmonic terms in the circumferential direction. The load stiffness matrix which accounts for variation of loads direction will be derived for each strip of the shell. Assembling of these matrices results in global load stiffness matrix which may be un-symmetric. Upon forming linear elastic stiffness matrix called constitutive stiffness matrix, geometric stiffness matrix and load stiffness matrix, the required elements for the second step analysis which is an eigenvalue problem are provided. In this study, different parameter effects are investigated including shell geometry, material properties, and different boundary conditions. Afterwards, the outcomes are compared with other researches. By considering the results of this article, it can be concluded that the deformation-dependent pressure assumption can entail to decrease the calculated buckling load in shells. This characteristic is studied for different examples.

• Restorative Dentistry and Endodontics
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• 제29권4호
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• pp.370-377
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• 2004

The purpose of this study was to regenerate human dental pulp tissues similar to native pulp tissues. Using the mixture of type I collagen solution, primary cells collected from the different tissues (pulp, gingiva, and skin) and NIH 3T3 ($1{\;}{\times}{\;}10^5{\;}cells/ml/well$) were cultured at 12-well plate at $37^{\circ}C$ for 14 days. Standardized photographs were taken with digital camera during 14 days and the diameter of the contracted collagen gel matrix was measured and statistically analyzed with student t-test. As one of the pulp tissue engineering, normal human dental pulp tissue and collagen gel matrix cultured with dental pulp cells for 14 days were fixed and stained with Hematoxyline & Eosin. According to this study, the results were as follows: 1. The contraction of collagen gel matrix cultured with pulp cells for 14 days was significantly higher than other fibroblasts (gingiva, skin) (p < 0.05), 2. The diameter of collagen gel matrix cultured with pulp cells was reduced to 70.4% after 7 days, and 57.1% after 14 days. 3. The collagen gel without any cells did not contract, whereas the collagen gel cultured with gingiva and skin showed mild contraction after 14 days (88.1% and 87.6% respectively). 4. The contraction of the collagen gel cultured with NIH 3T3 cells after 14 days was higher than those cultured with gingival and skin fibroblasts, but it was not statistically significant (72.1%, p > 0.05). 5. The collagen gel matrix cultured with pulp cells for 14 days showed similar shape with native pulp tissue without blood vessels. This approach may provide a means of engineering a variety of other oral tissue as well and these cell behaviors may provide information needed to establish pulp tissue engineering protocols.