• 생약학회지
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• 제47권3호
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• pp.237-245
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• 2016

In present study, we conducted quantification analysis of phenolic compound (paeonol), monoterpene glycoside (paeoniflorin), and tannin ((+)-catechin in the 70% ethanol extracts of non-processed Moutan Radicis Cortex (MRC) and processed MRC by roasting using a high-performance liquid chromatography coupled with photodiode array detector. Three marker components were separated on Gemini $C_{18}$ analytical column and the column was maintained at $40^{\circ}C$ using two mobile phase system consisting of 1.0% (v/v) aqueous acetic acid and 1.0% (v/v) acetic acid in acetonitrile. The flow rate and injection volume were 1.0 mL/min and 10 mL. In non-processed MRC sample, the concentrations of three marker compounds, (+)-catechin, paeoniflorin, and paeonol were 0.20, 1.18, and 2.12%, respectively. On the other hand, the concentrations of the three compounds in processed MRC samples were 0.03-0.24, not detected-1.08, and 0.76-1.82%, respectively.

• Clinical and Experimental Pediatrics
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• 제62권6호
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• pp.217-223
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• 2019

Purpose: To determine the diagnostic value of eosinopenia and the neutrophil-to-lymphocyte ratio (NLR) in the diagnosis of early onset neonatal sepsis (EONS). Methods: This cross-sectional study was conducted in the Neonatology Ward of R.D. Kandou General Hospital Manado between July and October 2017. Samples were obtained from all neonates meeting the inclusion criteria for EONS. Data were encoded using logistic regression analysis, the point-biserial correlation coefficient, chi-square test, and receiver operating characteristic curve analysis, with a P value <0.05 considered significant. Results: Of 120 neonates who met the inclusion criteria, 73 (60.8%) were males and 47 (39.2%) were females. Ninety (75%) were included in the sepsis group and 30 (25%) in the nonsepsis group. The mean eosinophil count in EONS and non-EONS groups was $169.8{\pm}197.1cells/mm^3$ and $405.7{\pm}288.9cells/mm^3$, respectively, with statistically significant difference (P<0.001). The diagnostic value of eosinopenia in the EONS group (cutoff point: $140cells/mm^3$) showed 60.0% sensitivity and 90.0% specificity. The mean NLR in EONS and non-EONS groups was $2.82{\pm}2.29$ and $0.82{\pm}0.32$, respectively, with statistically significant difference (P<0.001). The diagnostic value of NLR in the EONS group (cutoff point, 1.24) showed 83.3% sensitivity and 93.3% specificity. Conclusion: Eosinopenia has high specificity as a diagnostic marker for EONS and an increased NLR has high sensitivity and specificity as a diagnostic marker for EONS.

• 정신신체의학
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• 제31권1호
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• pp.19-24
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• 2023

Objectives : Biofeedback is a useful non-pharmacological treatment for panic disorder (PD), but no studies have identified physiological markers related to the treatment response. This study investigated predictors of the treatment response for biofeedback in patients with PD. Methods : A retrospective study based on the electronic medical records of 372 adult patients with PD was performed. Patients received biofeedback treatment at least once, and physiological markers including heart rate, heart rate variability, respiratory rate, skin conductance, skin temperature, and electromyography were collected before the treatment began. The patients were classified as responders or non-responders based on the change in Clinical Global Impression-Severity (CGI-S) score. Results : The response rate to biofeedback treatment was 30.4%. Multivariable logistic regression analysis revealed that a higher CGI-S score at baseline and fewer benzodiazepine prescriptions were associated with a better response to biofeedback treatment. According to subgroup analyses, the baseline CGI-S score, dose of benzodiazepines, and skin conductance are candidate predictors of the response to biofeedback treatment in men, while only baseline disease severity was associated with the treatment response in women. Conclusions : The present results suggest that skin conductance may be target marker and predictor for biofeedback in male patients with PD.

• Asian Pacific Journal of Cancer Prevention
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• 제13권11호
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• pp.5353-5361
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• 2012

Interleukin-18 (IL-18) is an immune-stimulatory cytokine with antitumor activity in preclinical models. It plays pivotal roles in linking inflammatory immune responses and tumor progression and is a useful candidate in gene therapy of lymphoma or lymphoid leukemia. A phase I study of recombinant human IL-18 (rhIL-18) in patients with advanced cancer concluded that rhIL-18 can be safely given in biologically active doses to patients with advanced cancer. Some viruses can induce the secretion of IL-18 for immune evasion. The individual cytokine activity might be potentiated or inhibited by combinations of cytokines. Here we focus on combinational effects of cytokines with IL-18 in cancer progression. IL-18 is an important non-invasive marker suspected of contributing to metastasis. Serum IL-18 may a useful biological marker as independent prognostic factor of survival. In this review we cover roles of IL-18 in immune evasion, metastasis and angiogenesis, applications for chemotherapy and prognostic or diagnostic significance.

• BMB Reports
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• 제51권2호
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• pp.57-58
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• 2018

An accurate diagnostic marker for detecting early-stage hepatocellular carcinoma (eHCC) is clinically important, since early detection of HCC remarkably improves patient survival. From the integrative analysis of the transcriptome and clinicopathologic data of human multi-stage HCC tissues, we were able to identify barrier-to-autointegration factor 1 (BANF1), procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (PLOD3) and splicing factor 3b subunit 4 (SF3B4) as early HCC biomarkers which could be detected in precancerous lesions of HCC, with superior capabilities to diagnose eHCC compared to the currently popular HCC diagnostic biomarkers: GPC3, GS, and HSP70. We then showed that SF3B4 knockdown caused G1/S cell cycle arrest by recovering $p27^{kip1}$ and simultaneously suppressing cyclins, and CDKs in liver cancer cells. Notably, we demonstrated that aberrant SF3B4 overexpression altered the progress of splicing progress of the tumor suppressor gene, kruppel like factor 4 (KLF4), and resulted in non-functional skipped exon transcripts. This contributes to liver tumorigenesis via transcriptional inactivation of $p27^{kip1}$ and simultaneous activation of Slug genes. Our results suggest that SF3B4 indicates early-stage HCC in precancerous lesions, and also functions as an early-stage driver in the development of liver cancer.

• 한국콘텐츠학회논문지
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• 제17권10호
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• pp.94-101
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• 2017

본 연구는 뇌졸중 편마비 환자 24명을 대상으로 마비측과 비마비측의 바이오 임피던스 값을 비교 분석하고자 하는 것이다. 본 연구에서는 2015년 10월부터 11월 까지 뇌졸중으로 진단 받은 편마비환자 24명을 대상으로 하였다. MultiScan 5000을 이용하여 바이오임피던스를 측정하였고 프래딕션마커(Prediction mark), 저항성분(resistance), 리액턴스(reactance), 위상각(phase angle)을 비교분석하였다. 뇌졸중 편마비 환자와 바이오임피던스 값의 비교분석을 위해 뇌졸중 편마비 가 아닌 일반인 6명의 오른쪽과 왼쪽을 비교분석하였다. 뇌졸중 편마비 환자의 마비측과 비마비측 부위에서 임피던스 값을 측정하여 정량화된 수치로 나타낸 결과 뇌졸중 편마비 환자의 마비측과 비마비측의 프래딕션마커(Prediction mark), 리액턴스(reactance), 위상각(phase angle)의 값의 유의한 차이를 보였다(p<0.05). 일반인을 대상으로 오른쪽과 왼쪽의 프래딕션마커(Prediction mark), 리액턴스(reactance), 위상각(phase angle)의 값의 유의한 차이를 보이지 않았다(p>0.05). 본 연구 결과를 통해 뇌졸중 편마비 환자의 마비측과 비마비측의 임피던스값의 유의한 차이가 있다는 것을 알 수 있었고 또한, 이를 통해 임상의 재활치료를 받는 뇌졸중 환자의 치료에 정량화된 수치로 측정할 수 있는 유용한 평가도구로서의 가능성을 제시하였다. 향후 연구에서는 임피던스 분석을 이용하여 뇌졸중환자의 마비측과 비마비측의 분석뿐만 아니라 다양한 대상군, 다양한 신체부위 그리고 재활치료 중재의 효과 등을 측정하는 연구가 필요할 것으로 보인다.

• Journal of Veterinary Science
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• 제22권6호
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• pp.79.1-79.14
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• 2021

Background: In contrast to human medicine, only a small number of serum tumor markers are established in veterinary medicine even though they are a non-invasive diagnostic tool. Objectives: This study examined whether survivin could be suitable as a potential canine serum tumor marker. Methods: This study measured the serum survivin concentrations of dogs with mammary tumors (n = 33), squamous cell carcinoma (n = 9), soft-tissue sarcoma (n = 18) and multicentric lymphoma (n = 22), using a commercially available, competitive immunoassay kit (BlueGene). The serum survivin concentrations were compared with those of a healthy control group (n = 20) and a control group of dogs with non-neoplastic diseases (n = 17). Results: Dogs with malignant tumors had serum survivin concentrations between 15 and 5,906 pg/mL (median, 72 pg/mL), those in the healthy group ranged from 7 to 99 pg/mL (median, 21 pg/mL) and those in the group of dogs suffering from non-neoplastic diseases from 15 to 93 pg/mL (median, 42 pg/mL). The differences in the survivin concentrations between the healthy dogs and dogs with malignant tumors and between the dogs with non-neoplastic diseases and those with malignant tumors were significant (p < 0.001 and p = 0.006, respectively). Conclusions: The serum survivin concentrations in dogs with malignant tumors, with some exceptions, are higher than in dogs with benign tumors and dogs that do not suffer from a malignancy. Therefore, survivin can provide information on the presence of malignant tumors and be used as a tumor marker in dogs.

• 한국작물학회지
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• 제51권5호
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• pp.458-465
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• 2006

Quality protein maize(QPM) 육성계통의 화학적 특성과 지방산 및 아미노산 조성을 검토하기 위하여 KS5/QPM 계통의 $BC_{1}F_{2}$ 및 KS135/QPM 계통의 $BC_{1}F_{2}$ 자식계통을 대상으로 opaque-2 specific micro-satellite marker인 umc1066를 사용하여 QPM 26계통을 선발하고 non-QPM 34계통을 공시하여 얻어진 결과를 요약하면 다음과 같다. 1. QPM계통은 non-QPM계통에 비하여 지방함량이 유의하게(p<0.05) 증가되었으나 단백질함량은 통계적 유의차가 없었다. 2. QPM 계통과 non-QPM 계통 모두 linoleic acid(Cl8:2)의 조성비가 가장 높고 oleic acid(Cl8:1), palmitic acid(Cl6:0), linolenic acid(Cl8:3), stearic acid(Cl8:0)의 순으로 지방산 조성비가 높았다. 3. QPM 계통은 non-QPM 계통에 비하여 포화지방산의 비율이 다소 높고 불포화지방산의 비율은 non-QPM 계통에 비하여 조성비가 낮았으나 통계적 유의성은 없었다. 4. QPM 계통은 non-QPM 계통에 비하여 lysine의 조성 비율이 높고 황함유 아미노산(cystine, methionine)의 비율이 증가되었다. 5. QPM 계통의 필수아미노산의 조성비율은 28.1%였고 non-QPM 계통은 27.1%로 QPM 계통이 영양학적인 면에서 유리한 것으로 판단되었다.

• Environmental Analysis Health and Toxicology
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• 제19권2호
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• pp.217-225
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• 2004

In order to evaluate a health effect of air pollution, we designed exposure group (taxi driver, street sweeper, street trader,) and non -exposure group (office clerk). We analysed exposure and biologic marker by using personal sampler. Mean NO$_2$ and benzene level in each group were statistically significant. Also, respiratory symptom, chronic cough, sputum, and dyspnea on exertion were statistically significant in each group.

• Journal of Microbiology and Biotechnology
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• 제14권4호
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• pp.783-788
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• 2004

Marker proteins of genetically-modified organisms (GMO) and their antibodies were prepared and characterized as major components of an analytical system. We selected two GMO markers, neomycin phosphotransferase II and 5- enolpyruvylshikimate-3-phosphate synthase, and produced them from E. coli employing genetic recombination technology. After purification, their structural conformation and binding affinities to the respective antibodies were characterized. The results showed that the recombinant proteins were identical with commercially obtained reference proteins. We further used them as immunogens to raise polyclonal antibodies capable of discriminating GMO containing protein from non-GMO. Well-characterized marker proteins and antibodies will be valuable as immunoreagents in constructing analytical systems such as biosensors and biochips to measure quantities of GMO.