• Title/Summary/Keyword: non-marine

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Development of Species-Specific PCR to Determine the Animal Raw Material (종 특이 프라이머를 이용한 동물성 식품원료의 진위 판별법 개발)

  • Kim, Kyu-Heon;Lee, Ho-Yeon;Kim, Yong-Sang;Kim, Mi-Ra;Jung, Yoo Kyung;Lee, Jae-Hwang;Chang, Hye-Sook;Park, Yong-Chjun;Kim, Sang Yub;Choi, Jang Duck;Jang, Young-Mi
    • Journal of Food Hygiene and Safety
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    • v.29 no.4
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    • pp.347-355
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    • 2014
  • In this study, the detection method was developed using molecular biological technique to distinguish authenticity of animal raw materials. The genes for distinction of species about animals targeted at Cytochrome c oxidase subunit I (COI), Cytochrome b (Cytb), and 16S ribosomal RNA (16S rRNA) genes in mitochondrial DNA. The species-specific primers were designed by that Polymerase Chain Reaction (PCR) product size was around 200 bp for applying to processed products. The target 24 raw materials were 2 species of domestic animals, 6 species of poultry, 2 species of freshwater fishes, 13 species of marine fishes and 1 species of crustaceans. The results of PCR for Rabbit, Fox, Pheasant, Domestic Pigeon, Rufous Turtle Dove, Quail, Tree Sparrow, Barn Swallow, Catfish, Mandarin Fish, Flying Fish, Mallotus villosus, Pacific Herring, Sand Lance, Japanese Anchovy, Small Yellow Croaker, Halibut, Jacopever, Skate Ray, Ray, File Fish, Sea Bass, Sea Urchin, and Lobster raw materials were confirmed 113 bp ~ 218 bp, respectively. Also, non-specific PCR products were not detected in compare species by species-specific primers. The method using primers developed in this study may be applied to distinguish an authenticity of food materials included animal raw materials for various processed products.

The Impact of Sand Addition to An Intertidal Area for the Development of the Manila Clam, Ruditapes philippinarum Habitat on Benthic Community Structure - the case of an sandbank in Gonam-myeon, Taean-gun - (바지락 치패발생장 조성을 위한 모래살포가 저서동물 군집구조에 미치는 영향 - 태안군 고남면 모래톱 갯벌 사례 -)

  • Yoon, Sang-Pil;Song, Jae-Hee;Kim, Youn-Jung;An, Kyoung-Ho
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.17 no.4
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    • pp.270-282
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    • 2012
  • This study was conducted to investigate the impact of sand addition to an intertidal for the development of the Manila clam habitat on benthic community structure. For this, we focused on the spatio-temporal changes in the surface sediment condition and benthic community structure before and after the event. Study site was an sandbank in Gonam-myeon, Taean-gun where sand added to on July 2010. We set three stations at each of sand adding area (experimental plot) and non sand-adding area (control plot) and did sampling works ten times from June 2010 to October 2011. Directly after the event, surface sediments changed to very coarse sand, but the state was not maintained over four months because of seasonal sedimentation and finally got back to very fine sand in eight months. The number of species and density were temporarily reduced right after the event and crustacean species such as Apocorophium acutum, Photis sp. were most negatively affected by the event. However, the number of species recovered from the reduction in three months and density did in four months due to the recolonization by the existing species and species in the vicinity of the plot. During the study period, dominant species continuously changed from the species such as A. acutum, Photis sp. at the time before the event, through the species such as Heteromastus filiformis, Macrophthalmus japonicus at the time right after the event, to the species such as Musculista senhousia, Ruditapes philippinarum, Mediomastus californiensis in the latter part of the study period. Although surface sediment properties and ecological indices recovered within a certain period after the event, the recovery of community structure has never been observed up to the end of the study.

Characterization and Modification of Milk Lipids (유지방의 특성과 변화)

  • Yeo, Yeong-Geun;Choe, Byeong-Guk;Im, A-Yeong;Kim, Hyo-Jeong;Kim, Su-Min;Kim, Dae-Gon
    • Journal of Dairy Science and Biotechnology
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    • v.16 no.2
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    • pp.119-136
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    • 1998
  • The lipids of milk provide energy and many essential nutrients for the newborn animal. They also have distinctive physical properties that affect the processing of dairy products. Milk fat globules mainly consist of neutral lipids like triacylglycerols, whereas the globule membranes contain the complex lipids mostly, Phospholipids are a small but important fraction of the milk lipids and are found mainly in the milk fat globule membrane and other membranous material in the skim-milk phase. The milk fats of ruminant animals are characterized by the presence of relatively high concentrations of short-chain fatty acids, especially butyric and hexanoic acids, which are rarely found in milks of non-ruminants. The fatty acids of milk lipids arise from de novo synthesis in the mammary gland and uptake from the circulating blood. The fatty acid compositions of milks are usually complex and distinctive, depending on the nature of the fatty acids synthesized de novo in the mammary gland and those received from the diet in each species. The content and composition of milks from different species vary widely; presumably, these are evolutionary adaptations to differing environments. The actual process by which these globules are formed is unkonwn, but there are indications that triglyceride-containing vesicles which bleb from endoplasmic reticulum may serve as nucleation sites for globules. Recent studies on milk have centred on the manipulation of milk lipids to increase specific fatty acids, i.e. 20-carbon omega-3 fatty acids (eicosapentaenoic acid 20:5n3, decosahexaenoic acid 22:6n3) from marine sources because the fatty acids are closely associated with a decreased risk of coronary heart disease.

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Structure and Physical Property of the Crust of Mid-west Korea: Analysis of Sedimentary Basins in the Namyang and Tando Areas, Kyeonggi Province, Korea (한반도 중서부 지각구조와 물성 연구: 경기도 화성군 남양 및 안산시 탄도지역에 분포하는 퇴적분지의 분석)

  • Park, Sung-Dae;Chung, Gong-Soo;Jeong, Ji-Gon;Kim, Won-Sa;Lee, Dong-Woo;Song, Moo-Young
    • Journal of the Korean earth science society
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    • v.21 no.5
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    • pp.563-582
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    • 2000
  • Two Cretaceous(80-90 Ma) non-marine sedimentary basins, Namyang and Tando Basins, are distributed in the Namyang area, Hwaseonggun and in the Tando area, Ansanshi, Kyungki Province, Korea. The Namyang and Tando Basins are composed of 10 facies, which are pooped into 5 facies associations(FA). FA I consists of massive conglomerate facies, normally graded conglomerate facies and reversely graded conglomerate facies, which is interpreted to have been formed by laminated sandstone facies, massive conglomerate facies(channelized), which is thought to have been formed by sheet flow, stream flow and suspension sedimentation in an alluvial/braided plain environment. FA III consists of massive mudstone(pebbly) facies, laminated mudstone facies, massive sandstone facies and is interbedded by channel-fill conglomerate. It is interpreted to have been deposited by suspension settling during flooding and channel-fill deposition in a floodplain environment. FA IV consists of massive conglomerate facies, normally graded conglomerate facies, massive sandstone facies, normally graded sandstone facies, and laminated sandstone facies and is interbedded with mudstone facies. It is thought to have been deposited by debris flow and turbidity current in a fan-delta environment. FA V consists of massive mudstone facies, laminated mudstone facies, laminated sandstone facies and is interbedded by massive conglomerate bed. It is thought to have been formed by suspension sedimentation and low-density turbidity current in a lake. In the Namyang Basin FA I is distributed in the eastern and southern margin of the basin, FA II in the middle part of the basin as north-south tending band. and FA III in the western part. In the Tando Basin FA II is distributed in the middle part of eastern margin and in the northwestern margin, FA IV in the southwestern part, and FA V in the central part. Correlation of the facies associations shows that FA I and II in the Namyang Basin are distributed in the lower to middle part of stratigraphic sequence and FA III in the upper part of the sequence whereas FA II and IV in the Tando Basin are in the lower to middle part and FA V in the upper part of the sequence. These patterns of facies associations distribution suggest that the Namyang Basin was developed as an alluvial fan and alluvial/braided plain at first and then evolved into a floodplain whereas the Tando Basin was developed as a fan-delta and alluvial/braided plain at first and then evolved into a lake environment.

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Culture of the Olive Flounder (Paralichthys olivaceus) in a Semi-closed Recirculating Seawater System (반폐쇄식 순환여과 사육시스템에서의 넙치 (Paralichthys olivaceus) 양식)

  • CHANG Young Jin;KIM Seung Hyern;YANG Han Soeb
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.28 no.4
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    • pp.457-468
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    • 1995
  • In order to develop a more practical culture system from the present running seawater tank system, two experiments of environmental factors, growth, survival rate and rearing density of olive flounder (Paralichthys olivaceus) were carried out for two consecutive years. Two groups of fish in initially averaging 7.5cm of total length, and 3.4g of body weight (EXP. I) and 5.0cm and 1.8g (EXP. II) were reared in the semi-closed recirculating seawater system equipped with the rotating biological contactors with the commercial culture scale. The dissolved inorganic nitrogen concentrations is EXP. I ranged 0,247-0.512 ppm of $NH_4-N$ (0.010-0.043 ppm of$NO_2-N$, and 0.108-0.342 ppm of $NO_3-N$, and those in EXP. II were 0.091-0.715 ppm, 0.002-0.045 ppm, and 0.007-0.277 ppm, respectively. Daily feeding rates of the fish were $0.67-2.41\%$ in EXP. I and $0.69-2.22\%$ in EXP_ II, and teed efficiency were $34.8-59.8\%\;and\;40.5-88.4\%$ in EXP. I and II, respectively. The average total ten說h and body weight were 40.0-42.8cm and 695.0-852.69g after 340 days culture in EXP. I, and 36.7-39.7cm and 552.4-706.4 g after 365 days culture in EXP. II, respectively. Survival rates of the fish at the end of EXP. I and II were $92.0\%\;and\;96.0\%,$ respectively. The ratio to body surface area of non-ocular side in all fish to bottom area of rearing tank, so-called covering rate, was used as an indicator of rearing density. The highest cowering rate and weight density of fish per $m^2$ of rearing tank at the end of experimental period were 2.2 and 34.1kg in EXP. I, and 2.6 and 36.3kg in EXP. II, respectively. For the commercial culture of olive flounder, the semi-closed recirculating seawater system was found to be more effective than the running seawater tank system in aspect to the fish productivity and protection of marine environment.

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The characteristic of InGaN/GaN MQW LED by different diameter in selective area growth method (선택성장영역 크기에 따른 InGaN/GaN 다중양자우물 청색 MOCVD-발광다이오드 소자의 특성)

  • Bae, Seon-Min;Jeon, Hun-Soo;Lee, Gang-Seok;Jung, Se-Gyo;Yoon, Wi-Il;Kim, Kyoung-Hwa;Yang, Min;Yi, Sam-Nyung;Ahn, Hyung-Soo;Kim, Suck-Whan;Yu, Young-Moon;Ha, Hong-Ju
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.22 no.1
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    • pp.5-10
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    • 2012
  • In general, the fabrications of the LEDs with mesa structure are performed grown by MOCVD method. In order to etch and separate each chips, the LEDs are passed the RIE and scribing processes. The RIE process using plasma dry etching occur some problems such as defects, dislocations and the formation of dangling bond in surface result in decline of device characteristic. The SAG method has attracted considerable interest for the growth of high quality GaN epi layer on the sapphire substrate. In this paper, the SAG method was introduced for simplification and fabrication of the high quality epi layer. And we report that the size of selective area do not affect the characteristics of original LED. The diameter of SAG circle patterns were choose as 2500, 1000, 350, and 200 ${\mu}m$. The SAG-LEDs were measured to obtain the device characteristics using by SEM, EL and I-V. The main emission peaks of 2500, 1000, 350, and 200 ${\mu}m$ were 485, 480, 450, and 445 nm respectively. The chips of 350, 200 ${\mu}m$ diameter were observed non-uniform surface and resistance was higher than original LED, however, the chips of 2500, 1000 ${\mu}m$ diameter had uniform surface and current-voltage characteristics were better than small sizes. Therefore, we suggest that the suitable diameter which do not affect the characteristic of original LED is more than 1000 ${\mu}m$.

Growth and Survival of the Spat of Arkshell, Scapharca broughtonii in Intermediate Culture with Different Shape of Protective Net and Type of Preventive Net of Spat Loss (피조개, Scaphara broughtonii 부착치패의 중간양성시 보호망 형태와 유실방지망 종류에 따른 성장과 생존)

  • Kim, Byeong-Hak;Shin, Yun-Kyung;Park, Ki-Yeol;Choi, Nack-Joong;Oh, Bong-Se;Min, Byeong-Hee
    • The Korean Journal of Malacology
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    • v.24 no.2
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    • pp.131-136
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    • 2008
  • Growth and survival of the spat of arkshell, Scapharca broughtonii were investigated for the improvement of survival in intermediate culture with different shape of protective net and type of preventive net of spat loss. The growth performance of the spat of arkshell was observed for 60 days by using different forms of protective net, as the exposure form with an average shell length of $12.8{\pm}3.2$ mm(P<0.05), the fish pot form with $12.2{\pm}3.5$ mm, the cylinder form with $11.9{\pm}3.8$ mm and the last one is the rectangular form with $10.9{\pm}3.7$ mm. Their numerical value did not show any marked difference with each other. But in case of survival rate, the fish pot form showed highest survival rate which is 43.7% and the significantly lowest was showed by the exposure form, which is 5.4%(P<0.05). The cylinder form and rectangular form showed 41.2 and 31.6% respectively. And then the growth of the spat of arkshell was observed for 163 days by rearing in a sort of preventive net, the first group was in a blackout curtain with average shell length of $13.9{\pm}3.1$ mm, the next group was in a balsam pear net with $12.9{\pm}3.0$ mm, in a polyethylene net with $11.8{\pm}3.1$ mm and the control with $12.6{\pm}3.3$ mm which was not installed by preventive net of spat loss. The survival rate was 91.5% in a blackout curtain, 90.1% in a polyethylene net, 88.5% in a balsam pear net and 61.5% in a controlled group. It is seen that the highest growth and survival rate were observed in a fish pot form and cylinder form. These results were a little bit difference from those of the spat of arkshell cultured in the form of different preventive nets. There was no significant difference in survival rate in relation to the sorts of preventive net against carrying-away, but there was a difference in survival rate by more than 30% as against the non-installed controlled group. We expect that survival rate would be highly improved in intermediate culture carried out with fish pot form and cylinder form of protective net and preventive net of spat loss.

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Analysis of Benthic Macroinvertebrate Community Structure and Stability in Major Inflow Streams of Lake Andong and Lake Imha (안동·임하호 주요 유입지천의 저서성 대형무척추동물 군집구조 및 군집안정성 분석)

  • You, Hyuk;Lee, Mi Jin;Seo, Eul Won;Lee, Jong Eun
    • Korean Journal of Environmental Biology
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    • v.34 no.4
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    • pp.320-328
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    • 2016
  • This study was conducted to provide important basic information about effective management of the marine environment at major inflow streams in Lake Andong and Lake Imha. The investigation was conducted 8 times from May, 2015 (AD1, AD2, IH1, IH2) to September, 2016 (AD3, AD4, IH3, IH4), and 8 surveyed sites were selected at Lake Andong (4 sites) and Lake Imha (4 sites). The inquiry identified 114 species, $59,913.7inds.\;m^{-2}$ in Lake Andong and 112 species, $39,038.4inds.\;m^{-2}$ in Lake Imha. The results indicate that the number of species and individuals in Lake Andong is more than that in Lake Imha, because Lake Andong has a variety of riparian vegetation and a richness of organic materials. Community analysis at Lake Imha revealed a dominant index of 0.57 (${\pm}0.18$), a diversity index of 2.87 (${\pm}0.31$), an evenness index of 0.73 (${\pm}0.04$), and a richness index of 4.17 (${\pm}0.71$). The results of functional feeding group analysis showed that a high proportion of species and individuals are gathering collectors. The results of functional habitat group analysis showed that a high proportion of species and individuals are clingers. The result of a physico-chemical water assay and dissolved oxygen and electric conductivity tests revealed that these measures increased when the water temperature decreased. The result of Pearson's correlation analysis by biological factors and physico-chemical factors showed that species and electric conductivity are highly correlated with one another. Major inflow streams of Lake Andong and Lake Imha were exposed to various point pollution sources and non-point pollution sources. This implies a necessity for continuous monitoring of the aquatic ecosystems in order to effect systematic water quality management of Lake Andong and Lake Imha.

Trace Metal Contents in Seaweeds from Korean Coastal Area (한국 연안 해조류의 미량금속 함량)

  • Kim, Ji-Hoe;Mok, Jong-Soo;Park, Hee-Yeon
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.34 no.7
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    • pp.1041-1051
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    • 2005
  • In order to assure the safety of the seaweeds, we measured the contents of the trace metals in the seaweeds harvested from Korean coastal area. We collected 620 marine algal samples from fourteen areas, and then analyzed the trace metals such as Hg, Cd, Cr, Cu, Mn, Ni, Pb and Zn. The contents of Hg, Cd, Ni and Mn were higher in the seaweeds collected from Chungnam Taean area. The contents of Cr and Cu were higher in the seaweeds collected from Yeongdeok and Tongyeong area, respectively. And Pb and Zn were highly detected from the seaweeds of Ulsan area. The mean levels of trace metals were high in the order of Zn $(48.02\pm41.20{\mu}g/g)\;>\;Mn (34.63\pm38.95{\mu}g/g)\;>\;Cu(6.29\pm6.52{\mu}g/g)\;>\;Ni(2.17\pm4.00{\mu}g/g)\; >\;Cr(1.61\pm2.35{\mu}g/g)\;>\;Pb(1.28\pm2.37{\mu}g/g)\;>\; Cd(0.67\pm0.75{\mu}g/g)\;>\;Hg(0.02\pm0.02{\mu}g/g)$. Zn was consistently most abundant metal in all samples, followed by Mn and Cu, which are very important for human health. There were higher contents of Hg and Cd in brown algae, the contents of Cr, Cu, Mn, Ni and Pb in green algae, the content of Zn in red algae among them. Additionally, significant linear correlations were observed between a total 28 different pairs of metals, of them, the correlation factor between Cr and Ni showed the highest (r=0.374, p<0.001). The trace metal contents of seaweeds varied with habitats and kinds of samples, nevertheless they showed very safe level in edible seaweeds tested. Scytosiphon lomentaria, which has been known as a little use of food, have shown a clear selectivity for Cr and Pb. Among non-edible seaweeds, Sargassum thunbergii for both Cd and Mn, Lomentaria hakodatensis for Ni and Grateloupia prolongata for Zn have shown a clear selectivity, respectively. Therefore, these seaweeds could be useful as bioindicator for each trace metal pollution.

Stratigraphy and Provenance of Non-marine Sediments in the Tertiary Cheju Basin (제주분지 제삼기 육성층의 층서 및 퇴적물 기원)

  • Kwon Young-In;Park Kwan-Soon;Yu Kang-Min;Son Jin-Dam
    • The Korean Journal of Petroleum Geology
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    • v.3 no.1 s.4
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    • pp.1-15
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    • 1995
  • Seismic reflection profiles and exploratory drilling well samples from the southern marginal-continental shelf basin of Korea delineate that the Tertiary sedimentary sequences can be grouped into five sequences (Sequence A, Sequence B, Sequence C, Sequence D and Sequence E, in descending order). Paleontologic data, K-Ar age datings, correlation with tuff layers and sequence stratigraphic analysis reveal that the sequences A, B, C, D and E can be considered as the deposits of Holocene $\~$ Pleistocene, Pliocene, Late Miocene, Early $\~$ Middle Miocene and Oligocene, respectively. The sequence stratigraphic and structural analyses suggest that the southern part of the Cheju Basin had experienced severe folding and faulting. NE-SW trending strike-slip movement is responsible for the deformation. The sinistral movement of strike-slip fault ceased before the deposition of Sequence B. Age dating and rare-earth elements analysis of volvanic rocks reveal+ that the Sequence D was deposited during the Early $\~$ Middle Miocene and the Sequence I was deposited earlier than the deposition of the Green Tuff Formation. Sedimentary petrological studies indicate that sediments of the Sequence I came from the continental block provenance. After the deposition of the Sequence E, uplift of the source area resulted in increase of sediment supply, subsidence and volcanic activities. The Sequence D show these factors and the sediments of the Sequence D are considered to be transported from the recycled orogenic belt.

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