• Preventive Nutrition and Food Science
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• v.11 no.4
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• pp.318-322
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• 2006

The objective of this experiment was to investigate the effect of extrusion of ginseng roots in twin screw extruder on susceptibility of ginseng starch toward hydrolysis by ${\alpha}-amylase$ BAN 480L (Novozyme, Denmark) and cellulase Celluclast 150L and saccharification by amyloglucosidase AMG-E (Novozyme, Denmark). The extrusion was conducted at 22% and 30% moisture contents of feed at screw speed 300 rpm. Barrel temperature at zone 1 was adjusted at $100^{\circ}C$ and $120^{\circ}C$. The results showed that extrusion process improved the ginseng ${\alpha}-amylase$ susceptibility as compared to traditionally dried ginseng (white and red ginseng). Reducing sugar of hydrolyzed extruded samples was 2,500% of its initial concentration, whereas the reducing sugar of hydrolyzed non-extruded sample was only 200% of its initial concentration. However, addition of cellulase during liquefaction lowered the saccharification yield of both non-extruded and extruded samples as well.

• Proceedings of the KSR Conference
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• 2003.10b
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• pp.170-175
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• 2003

The ASCE formula of pipeline' soil interaction force is the basis of semi-analytical relationship for buried pipelines subjected to longitudinal permanent ground deformation due to seismic induced liquefaction. However, since the ASCE formula has been developed based on the stiffness of non-liquefied region, it is needed to modify for the varied stiffness of liquefied region. With this object, the consideration of decreasing effect of soil stiffness in liquefied region is made: i.e. the spatial distributions of pipeline. soil interaction force in liquefied region. It means that the improved formula can reflect various patterns of permanent ground deformation more realistically. Through the comparative analyses using both the improved and ASCE formula, the applicability of the improved, the limitation of the existing formula and semi-analytical relationship are discussed.

• Proceedings of the Korean Geotechical Society Conference
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• 2010.03a
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• pp.480-488
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• 2010

The CGS method is non-discharge replacement method improving ground stiffness by the effect of static compaction with injecting very low slump mortar into ground, and is applied for increasing bearing capacity and filling ground cavity by lifting or restoring differential settled structures and preventing differential settlement. This paper suggests design of ground improvement and construction case history for civil engineering structures by CGS method. This method can be used for reinforcing soft ground and liquefaction of loose sandy soil. This method was used in SongDo area in Incheon Economic Free Zone due to its low vibration of ground while it can improve the soft soil where underground structures(subway and box culvert) are already existed.

• Clinical and Experimental Reproductive Medicine
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• v.49 no.4
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• pp.270-276
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• 2022

Objective: The present study assessed the biological characteristics of human spermatozoa at different time intervals (0, 1, 1.5, and 2 hours) after incubation at 37℃. Methods: Twenty-five normozoospermic semen samples were incubated at 37℃. Incubation was performed at four time intervals of 0 (after liquefaction), 1, 1.5, and 2 hours. The samples were evaluated for sperm parameters at each time interval. Results: The rate of sperm progressive motility decreased at 1.5 hours compared to 0 hours as well as 2 hours compared to 1 hour and 0 hours. The rate of non-motile spermatozoa also decreased after 2 hours compared to after 0 hours. No significant changes were observed in sperm viability (p=0.98) and non- progressive motility (p=0.48) at any time intervals. Abnormal sperm morphology increased at 1.5 hours of incubation time (p<0.001). No significant changes were observed in DNA fragmentation at 1 hour compared to 0 hours (median [interquartile range]: 19.5 [4] vs. 19 [4]), as well as at 1.5 hours compared to 1 hour (20 [5]). However, a significant increase in DNA fragmentation was observed at 1.5 hours compared to 0 hours. The mitochondrial membrane potential decreased remarkably after 1 hour of incubation time. No significant differences were observed in the acrosome reaction or malonaldehyde levels at any time point (p=0.34 and p=0.98, respectively). Conclusion: The incubation of normozoospermic samples before use in assisted reproductive technology should be less than 1.5 hours to minimize the destructive effects of prolonged incubation time on general and specific sperm parameters.

• Journal of Microbiology and Biotechnology
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• v.31 no.4
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• pp.570-583
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• 2021

Pyrococcus furiosus α-amylase can hydrolyze α-1,4 linkages in starch and related carbohydrates under hyperthermophilic condition (~ 100℃), showing great potential in a wide range of industrial applications, while its relatively low productivity from heterologous hosts has limited the industrial applications. Bacillus subtilis, a gram-positive bacterium, has been widely used in industrial production for its non-pathogenic and powerful secretory characteristics. This study was conducted to increase production of P. furiosus α-amylase in B. subtilis through three strategies. Initial experiments showed that co-expression of P. furiosus molecular chaperone peptidyl-prolyl cis-trans isomerase through genomic integration mode, using a CRISPR/Cas9 system, increased soluble amylase production. Therefore, considering that native P. furiosus α-amylase is produced within a hyperthermophilic environment and is highly thermostable, heat treatment of intact culture at 90℃ for 15 min was performed, thereby greatly increasing soluble amylase production. After optimization of the culture conditions (nitrogen source, carbon source, metal ion, temperature and pH), experiments in a 3-L fermenter yielded a soluble activity of 3,806.7 U/ml, which was 3.3- and 28.2-fold those of a control without heat treatment (1,155.1 U/ml) and an empty expression vector control (135.1 U/ml), respectively. This represents the highest P. furiosus α-amylase production reported to date and should promote innovation in the starch liquefaction process and related industrial productions. Meanwhile, heat treatment, which may promote folding of aggregated P. furiosus α-amylase into a soluble, active form through the transfer of kinetic energy, may be of general benefit when producing proteins from thermophilic archaea.

• Geomechanics and Engineering
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• v.28 no.5
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• pp.505-520
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• 2022

In recent years, geotextile-encased gravel columns (usually called stone columns) have become a popular method to increasing soil shear strength, decreasing the settlement, acceleration of the rate of consolidation, reducing the liquefaction potential and increasing the bearing capacity of foundations. The behavior of improved loose base-soil with gravel columns under shear loading and the shear stress-horizontal displacement curves got from large scale direct shear test are of great importance in understanding the performance of this method. In the present study, by performing 36 large-scale direct shear tests on sandy base-soil with different fine-content of zero to 30% in both not improved and improved with gravel columns, the effect of the presence of gravel columns in the loose soils were investigated. The results were used to predict the shear stress-horizontal displacement curve of these samples using support vector machines (SVM). Variables such as the non-plastic fine content of base-soil (FC), the area replacement ratio of the gravel column (Arr), the geotextile encasement and the normal stress on the sample were effective factors in the shear stress-horizontal displacement curve of the samples. The training and testing data of the model showed higher power of SVM compared to multilayer perceptron (MLP) neural network in predicting shear stress-horizontal displacement curve. After ensuring the accuracy of the model evaluation, by introducing different samples to the model, the effect of different variables on the maximum shear stress of the samples was investigated. The results showed that by adding a gravel column and increasing the Arr, the friction angle (ϕ) and cohesion (c) of the samples increase. This increase is less in base-soil with more FC, and in a proportion of the same Arr, with increasing FC, internal friction angle and cohesion decreases.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.10
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• pp.393-405
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• 2020

Various geotechnical information is required to evaluate the stability of the ground and a foundation once liquefaction occurs due to earthquakes, such as the soil strength and groundwater level. The results of the Standard Penetration Test (SPT) conducted in Korea are registered in the National Geotechnical Information Portal System. If geotechnical information for a non-drilled area is needed, geostatistics can be applied. This paper is about the feasibility of obtaining ground information by the Empirical Bayesian Kriging (EBK) method and the Inverse Distance Weighting Method (IDWM). Esri's ArcGIS Pro program was used to estimate these techniques. The soil strength parameter of the drilling area and the level of groundwater obtained from the standard penetration test were cross-validated with the results of the analysis technique. In addition, Multichannel Analysis of Surface Waves (MASW) was conducted to verify the techniques used in the analysis. The Buk-gu area of Pohang was divided into 1.0 km×1.0 km and 110 zones. The cross-validation for the SPT N value and groundwater level through EBK and IDWM showed that both techniques were suitable. MASW presented an approximate section area, making it difficult to clearly grasp the distribution pattern and groundwater level of the SPT N value.

• Horticultural Science & Technology
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• v.30 no.4
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• pp.409-416
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• 2012

The amount of cellular components including soluble sugars, amino acids, organic acids and glucosinolates (GLS) was investigated during radish root processing to develop a radish beverage. The radish root was divided into two parts, white and green tissue, and processed separately by extracting the juice from the fresh tissue and from the boiled tissue to compare differences in the components content among the preparations. The overall palatability of both the fresh and boiled extracts from the green part of the radish was higher than that of the same extracts from the white part. The sweetness of extract by boiling increased and its pungency decreased, thereby the palatability increased by being compared to the fresh radish extract. The sweetness was affected by sucrose not by glucose or fructose of monosaccharides by showing different sucrose contents according to treatment comparing palatability. Malic acid was identified as primary organic acid, and the content was higher in both the fresh and boiled extracts from the white part than in the extracts from the green part of the radish. The fresh extract from the green part of the radish contained more essential amino acids, such as threonine and valine, and more hydrophilic amino acids including glutamic acid, aspartic acid, and arginine than those of the fresh extract from the white part, suggesting the green fresh part is more palatable than the white fresh part. The main sulfur compound was ethylthiocyanate in radish, and others were butyl isothiocyanate, dimethyl-disulfide, and 4-methylthio-3-butylisothiocyanate. The four GLS were detected much more in the fresh green and fresh white parts of the radish because they evaporated during boiling. The contents of the four sulfur compounds were higher in the white fresh part than in the green fresh part, which is likely the reason the pungency was higher and the palatability was lower in the white fresh part than in the green fresh part of the radish. The ascorbic acid content was higher in the fresh extract compared to the boiled extracts from both the green and white parts. Taken together, these findings indicate that fresh radish extract is superior to obtain in terms of retaining desirable nutritional and functional components for health.

• Journal of the Korean Geotechnical Society
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• v.20 no.2
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• pp.15-26
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• 2004

Based on an estimating method for post-cyclic strength and stiffness with cyclic triaxial tests proposed by one of the authors, cyclic Direct Simple Shear (DSS) tests were carried out to confirm whether the method can be adapted to DSS test on fine-grained soils: silty clay, plastic silt, and non-plastic silt. Results from cyclic and post-cyclic DSS tests were interpreted by a modified method as adopted for cyclic and post-cyclic triaxial tests. In particular, influence of plasticity index for fine-grained soils and initial static shear stress (ISSS) was emphasised. Findings obtained from the present study are: (i) liquefaction strength ratio of fine-grained soils decreases with decreasing plasticity index and increasing ISSS; (ii) plasticity index and ISSS did not markedly influence relation between equivalent cyclic stiffness and shear strain relations; (iii) the higher the plasticity index of fine-grained soils is, the less the strength ratio decreases with increment of a normalcies excess pore water pressure (NEPWP); (iv) stiffness ratio of plastic silt has large activity decrease rapidly with increasing excess pore water pressure; and (v) post-cyclic strength and stiffness results from DSS tests agree well with those predicted by the method modified from a procedure used for triaxial test results.