• 대한소아치과학회지
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• 제42권1호
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• pp.102-111
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• 2015

진단기법의 급속한 발전으로 인해 타액은 의학과 치의학 분야에서 가장 중요한 진단 샘플로서의 가능성이 있다. 타액은 혈장과 비교할 때 다음과 같은 장점을 갖는다. 단순하고 비 침습적이며 큰 집단의 검사 시 경제적으로 이용할 수 있다. 샘플 채취 시 감염의 위험이 극히 적고, 임상적으로 샘플 채취가 어려운 어린이나 장애인, 불안한 환자에서 유용하게 사용될 수 있다. 이 종설은 타액의 진단적 이용 가능성에 대하여 고찰하고 최근의 연구에 대한 요약을 제공하고자 한다.

• 대한통합의학회지
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• 제5권1호
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• pp.75-83
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• 2017

Purpose : The parameters used in architectural analysis are muscle thickness, fascicle length, pennation angle, etc. Pennation angle is an important muscle characteristic that plays a significant role in determining a fascicle's force contribution to movement. Ultrasonography has been widely used to obtain the image for measurement of a pennation angle since it is non-invasive and real-time. However, manual assessment in ultrasonographic images is time-consuming and subjective, making it difficult for using in muscle function analysis. Thus, in this study, I proposed an automatic method to extract the pennation angle from the ultrasonographic images of gastrocnemius muscle. Method : The ultrasonographic image obtained from 10 healthy participants's gastrocnemius muscle using for developed automatic measuring program. Automatic measuring program algorithm consists with preprocessing, line detection, line classification, and angle calculation. The resulting image was then used to detect the fascicles and aponeuroses for calculating the pennation angle with the consideration of their distribution in ultrasonographic image. Result : The proposed automatic measurement program showed the stable repeatability of pennation angle calculation. Conclusion : This study demonstrated that the proposed method was able to automatically measure the pennation angle of gastrocnemius, which made it possible to easily and reliably investigate pennation angle more.

• 한국수정란이식학회지
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• 제14권1호
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• pp.1-8
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• 1999

The efficiency of transgenic livestock animal production may be improved by early selection of transgenci preimplantation embryos. To examine the possibility of GFP gene as a non-invasive marker for the early screening of transgenic embryo, the GFP gene was microinjected into rabbit zygotes and the later stages of preimplantation embryos were examined for the expression of GFP. The presence of injected DNA was detected by PCR analysis and the expression of GFP was detected by observing green fluorescence in embryos under a fluorescent microscope. Out of 108 GFP gene-injected rabbit zygotes, seventy three(67.6%) were fluorescence-positive. When 11 fluroresecence-positive blastocysts were analyzed for the presence of GFP gene by PCR, 6(54.5%) were positive, and all of the 8 flrouescence-negative blastocysts were also negative by PCR. The results indicate that the screening of transgene in rabbit embryos by PCR analysis and GFP detection could be a promising method for the preselection of transgenic embryos.

• 한국정보통신학회논문지
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• 제4권3호
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• pp.575-583
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• 2000

펄스 옥시메터는 수술실, 회복실, 집중 치료실 등에서 사용되는 산소포화도($SpO_2$)를 측정하는 방법 중 광흡수도를 이용하여 비관혈적인 방법으로 산소포화도륵 측정하는 장비이다. 펄스 옥시메터는 동맥혈의 광흡수도를 측정함으로써 혈액의 산소포화도를 나타낼 수 있다. 산소포화도를 측정하는 기존의 방법은 잡음을 제거하는 필터링 기술과 복잡한 처리 알고리즘, 그리고 많은 연산 수행 시간을 필요로 한다. 본 연구에서는 신호 검출 단계에서 적색광과 적외선광 각각의 AC 성분과 DC 성분을 분리하여 처리함으로써, 연산 알고리즘을 단순화 할 수 있었다. 그리고 시스템을 구현한 결과 기존의 방법(로그연산법, 미분법) 보다 속도향상과 0.3% 이상의 성능개선을 보였다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권6호
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• pp.2929-2932
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• 2014

Purpose: To evaluate the diagnostic performances of risk of malignancy index (RMI), CA-125 and ultrasound score in differentiating between benign and borderline or malignant ovarian tumors and find the best diagnostic test for referral of suspected malignant ovarian cases to gynaecologic oncologists. Materials and Methods: This prospective study covered 467 women with pelvic tumors scheduled for surgery at our hospital between July 2011 and July 2013. The RMI was obtained from ultrasound score, CA125 and menopausal status. The diagnostic values of each parameter and the RMI were determined and compared using Statistical Packages for Social Sciences Version 14.0.1. Results: In our study, 61% of ovarian tumors were malignant in the post-menopausal age group. RMI with a cut-off 150 had sensitivity of 84% and specificity of 97% in detecting ovarian cancer. CA-125>30 had a sensitivity of 84% and a specificity of 83%. An ultrasound score more than 2 had a sensitivity of 96% and specificity of 81%. RMI had the least false malignant cases thus avoiding unnecessary laparotomies. Ultrasound when used individually had the best sensitivity but poor specificity. Conclusions: Our study has demonstrated the RMI to be an easy, simple and applicable method in the primary evaluation of patients with pelvic masses. It can be used to refer suspected malignant patients to be operated by a gynaecologic oncologist. Other models of preoperative evaluation should be developed to improve the detection of early stage invasive, borderline and non-epithelial ovarian cancers.

• Journal of Forest and Environmental Science
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• 제34권1호
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• pp.71-76
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• 2018

The long-tailed goral (Naemorhedus caudatus) has slowly recolonized habitats in South Korea. Because it is necessary to know the status of groups in recolonized areas, we determined detection frequencies and group sizes using camera trapping, a non-invasive monitoring method. In Uljin, a far southern goral habitat in South Korea, we used a Moultrie 5.0 camera and mineral block as bait during the breeding season in BaekAm Mountain (148 days, 18 May to 11 October) and Geumjong Mountain (63 days, 18 May to 18 July) in 2010. Totally, 155 images were captured in BaekAm Mountain, whereas four images were captured in Geumjong Mountain. The species was most frequently detected at sunrise (05:00-08:00) and sunset (18:00-20:00). Through population structure evaluation, we identified at least 11 individuals, including one solitary mature male, four females, four kids, and two solitary subadults in BaekAm Mountain. However, in Geumjong Mountain, we identified only two individuals (female with kid). Monitoring efficiency in the recolonized area differed depending on population density and habitat conditions. Because we could evaluate the population structure, and behavioral patterns in the study sites, monitoring using camera traps could be applied for the recolonized habitats in South Korea.

• Biomolecules & Therapeutics
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• 제26권1호
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• pp.81-92
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• 2018

It is widely accepted that altered metabolism contributes to cancer growth and has been described as a hallmark of cancer. Our view and understanding of cancer metabolism has expanded at a rapid pace, however, there remains a need to study metabolic dependencies of human cancer in vivo. Recent studies have sought to utilize multi-modality imaging (MMI) techniques in order to build a more detailed and comprehensive understanding of cancer metabolism. MMI combines several in vivo techniques that can provide complementary information related to cancer metabolism. We describe several non-invasive imaging techniques that provide both anatomical and functional information related to tumor metabolism. These imaging modalities include: positron emission tomography (PET), computed tomography (CT), magnetic resonance imaging (MRI), magnetic resonance spectroscopy (MRS) that uses hyperpolarized probes and optical imaging utilizing bioluminescence and quantification of light emitted. We describe how these imaging modalities can be combined with mass spectrometry and quantitative immunochemistry to obtain more complete picture of cancer metabolism. In vivo studies of tumor metabolism are emerging in the field and represent an important component to our understanding of how metabolism shapes and defines cancer initiation, progression and response to treatment. In this review we describe in vivo based studies of cancer metabolism that have taken advantage of MMI in both pre-clinical and clinical studies. MMI promises to advance our understanding of cancer metabolism in both basic research and clinical settings with the ultimate goal of improving detection, diagnosis and treatment of cancer patients.

• 대한기계학회:학술대회논문집
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• 대한기계학회 2003년도 추계학술대회
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• pp.222-227
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• 2003

Recent advances in room-temperature, visible and near-IR diode laser sources for telecommunication, optical data storage applications are enabling combustion diagnostics system based on diode laser absorption spectroscopy. In contrast to some traditional sampling-based gas-sensing instruments, tunable diode laser absorption spectroscopy system is advantageous because of their non-invasive nature, high sensitivity, fast response time and real-time measurement capability. So, combined with fiber-optics and high sensitive detection strategies, compact and portable sensor system arc now appearing for a variety of applications. The objective of this research is to take advantage of distributed feed-back diode laser and measure the $CO_{2}$ concentration (by using direct absorption and wavelength modulation spectroscopy methods). In addition to survey spectra of $CO_{2}$ bands and spectroscopic parameters between 1565 and 1579 run were computed at temperatures between 296 and 1200 K (by using HITRAN 2000 database). It experimentally found out that the features of direct absorption and wavelength modulation spectroscopy methods.

• Molecules and Cells
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• 제23권2호
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• pp.132-137
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• 2007

With the advance of stem cell transplantation research, in vivo cell tracking techniques have become increasingly important in recent years. Magnetic resonance imaging (MRI) may provide a unique tool for non-invasive tracking of transplanted cells. Since the initial findings on the stem cell migration by MRI several years ago, there have been numerous studies using various animal models, notably in heart or brain disease models. In order to develop more reliable and clinically applicable methodologies, multiple aspects should be taken into consideration. In this review, we will summarize the current status and future perspectives of in vivo cell tracking technologies using MRI. In particular, use of different MR contrast agents and their detection methods using MRI will be described in much detail. In addition, various cell labeling methods to increase the sensitivity of signals will be extensively discussed. We will also review several key experiments, in which MRI techniques were utilized to detect the presence and/or migration of transplanted stem cells in various animal models. Finally, we will discuss the current problems and future directions of cell tracking methods using MRI.

• 세라미스트
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• 제22권1호
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• pp.70-81
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• 2019

Breath analysis is rapidly evolving as a non-invasive disease recognition and diagnosis method. Metal oxide gas sensors are one of the most ideal platforms for realizing portable, hand-held breath analysis devices in the near future. This paper reviewed the recent developments in metal oxide gas sensors detecting exhaled biomarker gases such as nitric oxides, acetone, ammonia, hydrogen sulfide, and hydrocarbons. Emphasis was placed on strategies to tailor sensing materials/films capable of highly selective and sensitive detection of biomarker gases with negligible cross-response to ethanol, the major interfering breath gas. Specific examples were given to highlight the validity of the strategies, which include optimization of sensing temperature, doping additives, utilizing acid-base interaction, loading catalysts, and controlling gas reforming reaction. In addition, we briefly discussed the design and optimization method of gas sensor arrays for implementing the simultaneous assessment of multiple diseases. Breath analysis using high-performance metal oxide gas sensors/arrays will open new roads for point-of-care diagnosis of diseases such as asthma, diabetes, kidney dysfunction, halitosis, and lung cancer.