• KSBB Journal
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• 제27권5호
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• pp.295-300
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• 2012

Matured dendritic cells (DCs) begin migration with their release from the bone marrow (BM) into the blood and subsequent traffic into peripheral lymphoid and non-lymphoid tissues. Throughout this long movement, migrating DCs must apply specialized skills to reach their target destination. Non-invasive in vivo cell-tracking techniques are necessary to advance immune cell-based therapies. In this study, we used a DiD cell-tracking solution for in vivo dendritic cell tracking in naive mice. We tracked DiD (non-invasive fluorescence dye)-labeled mature dendritic cells using the Near Infrared (NIR) imaging system in normal mice. We examined the immunophenotype of DiD-labeled cells compared with non-labelled mature DCs, and obtained time-serial images of NIR-DC trafficking after mouse footpad injection. In conclusion, we confirmed that DiD-labeled DCs migrated into the popliteal lymph node 24 h after the footpad injection. Here, these data suggested that the cell tracking system with the stable fluorescence dye DiD was useful as a cell tracking tool to advance dendritic cell-based immunotherapy.

• Journal of Acupuncture Research
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• 제30권5호
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• pp.1-9
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• 2013

Objectives : The purpose of this study was to present clinical utility of therapeutic exercise on the neck and shoulder parts based on the movement system impairment syndrome(MSIS) as Dong-Qi therapy of the Dong-Si Acupuncture and was to examine which Dong-Si acupoints were most effective and non-invasive when performing therapeutic exercise of the MSIS. Methods : Totally eight therapeutic exercises correspondent to eight neck and shoulder MSIS were summarized and tabulated from the Diagnosis and Treatment of Movement Impairment Syndrome and Movement System Impairment Syndromes Of The Extremities, Cervical and Thoracic Spines by Sahrmann SA. Together with the MSIS summaries, acupuncture points and Dong-Qi therapy were summarized and tabulated from Yangweijiequanji 1 and Yangweijiequanji 2 by Yangweijie. According to the posture and movement of the MSIS exercise, effective and non-invasive acupoints were selected. Thereafter, clinical pilot study which five normal volunteers participated in were performed to examine whether these acupoints resulted in any side effects of acupuncture therapy such as pain and distortion of the needle during the MSIS exercises. Results : Through clinical pilot study, ZhongZi, ZhongXian, ZhengJin, ZhengZong and MuLiu in a sitting position, and HuaGuYi, ZhongZi and ZhongXian in a supine position were finally determined as safe and non-invasive Dong-Si acupoints to treat cervical pain. In terms of shoulder pain, ZuQianJin, ZuWuJin, HuaGuEr and JianZhong in a supine position, SiHuaZhong, ZuQianJin, ZuWuJin, QuLing, JianZhong, ShenGuan and JiuLi in a standing position, and ZuQianJin, ZuWuJin, HuaGuEr, QuLing and JianZhong in a prone position were finally accepted as safe and non-invasive Dong-Si acupoints. Conclusion : It is concluded that Dong-Si acupoints can be safely and non-invasively used together with therapeutic exercises of the MSIS to treat cervical and shoulder pains.

• 대한화장품학회지
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• 제23권3호
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• pp.39-47
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• 1997

Retinol as well as RA is well known to have many beneficial effects on aged skin. But the skin irritation potential and unstable condition of the products containing them have been some problems in their cosmetic uses. So, retinol containing gel product was developed for less skin irritancy and more stability in cosmetic products. To examine the clinical effects of retinlo containing product, we used cilnicla non-invasive assessment techniques on 40 volunteers for 6 months maintaining double-blind test conditions. According to our results, the use of retinol containing product improved skin color and hydration level slightly. But there was no statistical differences. There was no erythema reaction compared to the use of RA. Especially, the skin elasticity increased above 20% and skin wrinkles of crows' feet region decreased more than 10%. Besides the instrumental analysis, a large majority of volnteers felt that their skin were improved in the case of wrunkles, elasticity, hydration and color.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 춘계학술발표대회
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• pp.355-356
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• 2000

Three 'stress probe' plasmids were constructed and characterized which utilize a green fluorescent protein (CFP) as a non-invasive reporter to elucidate Escherichia coli cellular stress responses in quiescent or 'resting' cells. Facile detection of cellular stress levels was achieved by fusion of three heat shock stress protein promoter elements, those of the heat shock transcription factor ${\sigma}^{32}$, pretense subunit ClpB, and chaperone DnaK, to the reporter gene $gfp_{uv}$. When perturbed by chemical or physical stress (such as heat shock, nutrient (amino acid) limitation, addition of IPTG, acetic acid, ethanol, phenol, antifoam, and salt (osmotic shock), the E. coli cells produced GFPuv which was easily detected from within the cells as emitted green fluorescence. A temporal and amplitudinal mapping of these responses was performed, demonstrating regions where quantitative delineation of cell stress was afforded.

• 한국감성과학회:학술대회논문집
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• 한국감성과학회 2000년도 춘계 학술대회 및 국제 감성공학 심포지움 논문집 Proceeding of the 2000 Spring Conference of KOSES and International Sensibility Ergonomics Symposium
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• pp.99-103
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• 2000

There are two approaches in the study of emotion in the physiological psychology. The first is to clarify the brain mechanism of emotion, and the second is to evaluate objectively emotions using physiological responses along with our feeling experience. The method presented here belongs to the second one. Our method is based on the "level-crossing point detection" method. which involves the analysis of frequency fluctuations of EEG and is characterized by estimation of emotionality using coefficients of slopes in the log-power spectra of frequency fluctuation in alpha waves on both the left and right frontal lobe. In this paper we introduce a new theory of estimation on an individual's emotional state by using our non-invasive and easy measurement apparatus.

• International Journal of Contents
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• 제9권1호
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• pp.38-41
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• 2013

The clamp meter maintains electric safety as a non-invasive method while measuring the absolute value of tube current with it has been recently developed for an X-ray high-tension cable. Especially this can show high accuracy at short X-ray exposure time. Considering such a condition, this study is to evaluate the feasibility of a clamp meter in measuring X-ray tube current by taking the measurements and comparing with those of the Dynalyzer III which has been considered as a standard measuring device. From measuring the tube current accuracy depending on changes in tube voltage and exposure time, the clamp meter showed higher accuracy rate which was -1.3~4.2% difference. Thus clamp meter can be used for clinical radiologists who are not familiar electric circuit to manage X-ray devices easily and correctly in the future.

• 대한의용생체공학회:의공학회지
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• 제33권3호
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• pp.114-127
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• 2012

Recent technical advancement allows noninvasive measurement of blood glucose. In this literature, we reviewed various noninvasive techniques for measuring glucose concentration. Optical or electrical methods have been investigated. Optical techniques include near-infrared spectroscopy, Raman spectroscopy, optical coherence technique, polarization, fluorescence, occlusion spectroscopy, and photoacoustic spectroscopy. Electrical methods include reverse iontophoresis, impedance spectroscopy, and electromagnetic sensing. Ultrasound, detection from breath, or fluid harvesting technique can be used to measure blood glucose level. Combination of various methods is also promising. Although there are many interesting and promising technologies and devices, there need further researches until a commercially available non-invasive glucometer is popular.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.407.3-408
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• 2002

A portable near infrared (NIR) system which was newly integrated by our lab has been used to develop a non-invasive blood glucose monitoring. The portable NIR system includes a tungsten halogen lamp, a photo diode array type-InGaAs detector, and specialized reflectance fiber optic probes. The shape of probes is composed of two parts. one for illumination into sample and the other for receiving the radiation from sample. Three kinds of probes with different distance between illumination and receiving part. such as 0.03. 0.1. and 0.5mm, were investigated for optimization. (omitted)

• Journal of Species Research
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• 제1권2호
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• pp.240-248
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• 2012

We used a non-invasive technique with microsatellite primers to investigate genetic variation among Eurasian otters Lutra lutra in eastern South Korea. We collected twenty two otter spraints in January and six in August 2008. We used spraints from five dead otters from five different river systems for the present genetic analysis. We extracted DNA from 20 spraints from the January sample. Ten microsatellite primers (Lut435, Lut453, Lut457, Lut604, Lut615, Lut701, Lut715, Lut717, Lut733, and Lut832) for Eurasian otters were tested, and four loci were successfully amplified for further analyses. The results of genotyping the otter population with microsatellite loci lead to the identification of 9 individuals from the Ungokcheon Stream. The Ungokcheon population also showed a genetic structure represented by the Hardy-Weinberg equilibrium.

• 대한의용생체공학회:의공학회지
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• 제18권4호
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• pp.407-412
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• 1997

In this paper, we present a new method to diagnose the characteristics of the soft tissue, especially a liver. In order to diagnose the characteristics of a liver, it is necessary to evaluate the propagation delay time and propagation velocity of bending vibration In a liver. For this purpose, we measure the propagation velocity of bending vibration in a liver for low frequency forced vibration using a standard ultrasonic Doppler diagnosis equipment. We have carried out preliminary experiments by using an ultrasonic probe of 3.5MHz and obtained some results. This new measurement method developed here can be applied to new research and medical fields for acoustic non-invasive diagnosis of soft tissue.