• KOREAN JOURNAL OF CROP SCIENCE
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• v.60 no.3
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• pp.333-337
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• 2015

This experiment was carried out to figure out the growth and yield of waxy corn after cultivating the hairy vetch which was mostly used as a green manure crop. The waxy corn showed the growth and yield efficiency relative to conventional culture after the hairy vetch was returned to as the green manure and the amount of nitrogen fertilizer increased, and the LAI was similar to the conventional culture when less than 18 kg. Length of ear slightly decreased compared to 188 mm of conventional culture when the hairy vetch was used as the green manure and the amount of nitrogen fertilizer was less than 9 kg, but the length of ear increased relative to the conventional culture when the amount of applied nitrogen fertilizer was more than 18 kg. The highest 100-kernel weight was 35.4 g with the hairy vetch and 27 kg of nitrogen fertilizer treatment. The lowest 100-kernel weight was 27.4 g with the hairy vetch and 0 kg of nitrogen fertilizer treatment. Quantity with the hairy vetch and 9 kg of the nitrogen fertilizer treatment was similar the conventional culture. Suger content with the hairy vetch and more than 18 kg of the nitrogen fertilizer treatment was higher than the conventional culture. Thickness of pericarp showed no difference among treatments.

• Korean Journal of Soil Science and Fertilizer
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• v.34 no.6
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• pp.401-406
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• 2001

This study was conducted to find out the effect of slow-release nitrogen fertilizer, such as latex coated urea(LCU) and Meister10(MS10) on direct seeded rice in flooded soil(FS). Junghwabyeo, and early maturity rice cultivar grown on the plots which were treated with None-nitrogen, urea, LCU and MS10 plot. Growth characteristic, yield and yield components were investigated. Nitrogen uptake-efficiency and physico-chemical properties of soil before-after experiment were analyzed. Plant height and number of tillers $m^{-2}$ in LCU and MS10 plot at early grow stages were higher than those of Urea plot. MS10 and LCU plot showed similar tendency on the plant height but MS10 plot was higher than LCU plot on the number of tillers $m^{-2}$. The number of seedling $m^{-2}$ were no significant differences among None-N, urea, and MS10 plot. Heading date and leaf color were no significant differences among None-N, urea, and MS10 plot. Culm length in LCU and Ms10 plot were longer compared with urea plot, but panicle length were similar among with Urea, LCU and MS10 plot. Number of panicles $m^{-2}$ was highest in MS10 plot and it was similar between LCU and Urea plot. Yield were greater in order of Ms10 > LCU > Urea plot. Nitrogen uptake and nitrogen efficiency were greater in order of MS10 > LCU > urea plot. After the experiment, total content of nitrogen in soil was not changed at all treatments, but pH, P and K of soil were lower than that of before experiment at all treatments.

• Journal of the Korea Organic Resources Recycling Association
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• v.6 no.2
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• pp.81-93
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• 1998

In order to estimate the an adequate application level for dry matter production of orchardgrass(Dactylis glomerata L.) were investigated in different application levels of food waste compost and mineral nitrogen in 3 cuttings per annum, and to evaluated the soil improving effect of food waste compost. Annual food waste compost and mineral nitrogen were applied at levels of 0, 10, 20, 40 and $60ton\;ha^{-1}$, and 0, 90, 180 and $270kg\;ha^{-1}$, respectively. Significantly higher dry matter yield of orchardgrass obtained were ranges of $8.92{\sim}9.70ton\;ha-1$ at levels of $180{\sim}270kg\;ha^{-1}\;yr^{-1}$ than that of other levels of mineral nitrogen. Relative yield of each cut to annual dry matter yield were 32.0% 49.2% and 18.8% for 1st cut, 2nd cut and 3rd cut in mineral nitrogen treatment. Significantly higher dry matter yield of orchardgrass obtained were ranges of $8.04{\sim}8.90ton\;ha^{-1}$ at levels of $20{\sim}60ton\;ha^{-1}\;yr^{-1}$ than that of other levels of food waste compost. The efficiency of dry matter production to application of mineral nitrogen(kg DM $kg^{-1}$ N) were 21.2, 19.0 and 15.6kg at levels of 90, 180 and $270kg\;ha^{-1}\;yr^{-1}$, respectively. Higher efficiency of dry matter Production obtained were 27.6~20.2 kg at levels of $90{\sim}180kg\;ha^{-1}$ of mineral nitrogen applied to $20ton\;ha^{-1}$ of food waste compost, it may due to accelerated mineralization by mineral nitrogen application. Highest efficiency of dry matter production to application of food waste compost (kg DM $ton^{-1}$ FWC) obtained was 71.0 kg at level of $40ton\;ha^{-1}\;yr^{-1}$. Maximum dry matter yield of orchardgrass obtained were $9.98ton\;ha^{-1}$ at limiting level of mineral nitrogen of $358.5kg\;ha^{-1}$ and $9.12ton\;ha^{-1}$ at limiting level of food waste compost of $49.3ton\;ha^{-1}$ per annum, respectively. Ranges of $20{\sim}49.3ton\;ha^{-1}$ of food waste compost and $180{\sim}358.5kg\;ha^{-1}$ of mineral nitrogen were estimated an adequate levels for increase in dry matter production, and to maintenance for orchardgrass pastures. Application of food waste compost was affected to improve the soil characteristics.

• Microbiology and Biotechnology Letters
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• v.17 no.6
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• pp.624-628
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• 1989

A study on the citric acid production using Saccharomycopsis lipolytica (NRRL Y7576) was carried out in shake-flasks, air-lift and membrane recycle bioreactors. The cells entrapped in Ca-alginate beads were used in shake-flasks and air-lift reactor. Repeated batch fermentation in shake-flasks was successfully performed for 34 days and resulted in a yield of 54%. Increased yield (63%) was obtained in the air-lift reactor operation using nitrogen deficient medium (NDM). In the membrane recycle bioreactor operation, the maximal dry cell mass concentration was 39 g/1 at a dilution rate of 0.02 h$^{-1}$ and the yield with NDM was higher than that with growth medium. In addition, the yield and volumetric productivity with pure oxygen supply were greatly improved compared with those with air supply.

• Journal of Microbiology
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• v.34 no.3
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• pp.255-262
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• 1996

The lipopolysaccharide (LPS) yields were measured in Rhodobacter capsulatus under several conditions by the ELISA method. The purification of LPS was done by affinity chromatography of IgG coupled CNBr-activated sepharose-4B instead of ultra-centrifugation. The purity of the LPS didn't show much difference between affinity chromatography and ultra-centrifugation method, but affinity chromatography method required much fewer organisms and was more convenient. LPS yield was measured in ng units by the ELISA method. Mannitol was a better single carbon source than other sugars, but mixing two carbon sources resulted in greater LPS yields than any sugar alone. LPS yield was directly proportional to $NH_ 4CI$ concentration, with optimum yields at 0.05% nitrogen. In contrest to LPS yields, which decreased at 0.005% nitrogen concentration total protein was increased 16 times. Calcium influenced LPS yields. At 0.7 mM $CaCI_ 2$, the LPS yield was 16.5 $\mu$g/mg DW, five times the yield without calcium.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.22 no.2
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• pp.42-47
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• 1977

Experiment was conducted to find out the influence of nitrogen and potassium split application on the yield and physiology of rice plants in a sandy-gravel soil. Nitrogen and potassium mixed application, nitrogen split application, and potassium split application increased yield of rice by 22, 20, and 18%, respectively, compared to conventional fertilizer application. Nitrogen split application increased chlorophyll content and potassium split application increased root activity. Concentration of silica, magnesium, and calcium was increased by the mixed split application of nitrogen and potassium.

• Asian-Australasian Journal of Animal Sciences
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• v.4 no.4
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• pp.369-375
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• 1991

A series of in vitro incubation studies with washed rumen bacteria were conducted to determine the influence of incubation time and concentrations of peptides, alanine, ammonia nitrogen and carbohydrate on the rate of peptide disappearance and on bacterial growth. Disappearance rate of tri-alanine (ala3) under various conditions was between 30.6 and $58.2mg\;hr^-$ per gram bacterial dry matter. Ala3 was removed from the incubation medium in an almost linear fashion as incubation time and ala3 concentration was increased. Washed rumen bacteria utilized ala3 faster than di-l-alanine (ala2) at all concentrations. Adding 9mM carbohydrate significantly increased ala3 disappearance, but level of ammonia nitrogen had no influence on ala3 disappearance. The presence of alanine in the medium significantly lowered ala3 utilization by rumen bacteria. Bacterial dry matter and nitrogen growth yield were not influenced by alanine and peptides when incubation medium already contained a sufficient level of ammonia nitrogen. Increased ammonia nitrogen in the presence of ala3 did not stimulate bacterial growth. Carbohydrate significantly increased bacterial dry matter and nitrogen growth as expected. Results indicate that the rate of peptide utilization by rumen bacteria may be altered by type and concentration of peptides, and energy supply, and this may be mediated through changes in numbers and type of bacteria.

• Proceedings of the Korean Society of Crop Science Conference
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• 1992.05a
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• pp.52-53
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• 1992

• KOREAN JOURNAL OF CROP SCIENCE
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• v.45 no.6
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• pp.400-404
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• 2000

Hairy vetch (vicia villosa Roth), leguminous green manure crop, can increase soil fertility and reduce chemical nitrogen application for cash crop such as spring corn. More yield of hairy vetch is needed at planting cash crop to obtain higher effect of green manure. Hairy vetch waa seeded on Sep.10, Oct.1, and Oct. 20 and at 10, 20, 30 and 40 kg/ha of seeding rate respectively, in 1996 and 1997, Dry matter and nitrogen yield of hairy vetch were measured on May 1 in 1997 and 1998. Proper seeding rate of hairy vetch was 30 kg/ha irrespective of years and seeding dates. Above-ground dry matters of hairy vetch on May 1 in 1997 and 1998 decreased according to delayed seeding, and those were 5.5-7, 4-4.5, 1.3-2.2 ton/ha on Sep. 10, Oct. 1 and Oct 20 of seeding date, respectively in seeding rate 30-40 kg/ha. Also nitrogen yield of hairy vetch on May 1 decreased according to delayed seeding, and those were 220-280, 160-180, 60-100kg/ha on Sep. 10, Oct. 1 and Oct 20 of seeding date, respectively in seeding rate 30-40kg/ha. Therefore, we suggest that hairy vetch has to be seeded earlier in autumn to obtain high green manure yield in spring. To determine the detailed optimum seeding time in autumn, hairy vetch was seeded on Aug. 20, Aug. 31, Sep. 10, Sep. 20, and Sep. 30 in 1999 and was harvested on April 22, April 27, and May 2 in 2000, respectively. Dry matter and nitrogen yield of hairy vetch by seeding in late August were higher than those by seeding in September indicating that dry matter of hairy vetch were 7-8, 6-7, 4-5, 2-3 ton/ha and nitrogen yield were 240-290, 200-260, 150-220, 70-120 kg/ha, respectively when seeded on Aug. 20, Aug. 31, Sep. 10, Sep. 20 and Sep. 30 and harvested on April 22-May 2. Increase of dry matter and N yield of hairy vetch by 10days delayed harvest was higher in late August seeding than in September seeding. So hairy vetch should be seeded in late August if possible to obtain much more green manure yield and be seeded until September because green manure yield decrease rapidly when seeded after October.

• Molecules and Cells
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• v.23 no.1
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• pp.72-79
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• 2007

Demand for low-input sustainable crop cultivation is increasing to meet the need for environment-friendly agriculture. Consequently, developing genotypes with high nutrient use efficiency is one of the major objectives of crop breeding programs. This study was conducted to identify QTLs for traits associated with physiological nitrogen use efficiency (PNUE). A recombinant inbred population (DT-RILs) between Dasanbyeo (a tongil type rice, derived from an indica ${\times}$ japonica cross and similar to indica in its genetic make-up) and TR22183 (a Chinese japonica variety) consisting of 166 $F_8$ lines was developed and used for mapping. A frame map of 1,409 cM containing 113 SSR and 103 STS markers with an average interval of 6.5 cM between adjacent marker loci was constructed using the DT-RILs. The RILs were cultivated in ordinary-N ($N-P_2O_5-K_2O=100-80-80kg/ha$) and low-N ($N-P_2O_5-K_2O=50-80-80kg/ha$) (100 kg/ha) conditions. PNUE was positively correlated with the harvest index and grain yield in both conditions. Twenty single QTLs (S-QTLs) and 58 pairs of epistatic loci (E-QTLs) were identified for the nitrogen concentration of grain, nitrogen concentration of straw, nitrogen content of shoot, harvest index, grain yield, straw yield and PNUE in both conditions. The phenotypic variance explained by these S-QTLs and E-QTLs ranged from 11.1 to 44.3% and from 16.0% to 63.6%, respectively. The total phenotypic variance explained by all the QTLs for each trait ranged from 35.8% to 71.3%, showing that the expression of PNUE and related characters depends signify- cantly upon genetic factors. Both S-QTLs and E-QTLs may be useful for marker-assisted selection (MAS) to develop higher PNUE genotypes.