• Title/Summary/Keyword: nitrogen species

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Electrical Characteristic and Optical Diagnosis for Atmospheric Direct Plasma Jet

  • Hong, Seong In;Ghimire, B.;Hong, Young Jun;Choi, Eun Ha
    • Proceedings of the Korean Vacuum Society Conference
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    • 2015.08a
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    • pp.155.1-155.1
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    • 2015
  • Nowadays, Plasma has been used in biological, medical such as wound healing, plant grow, killing cancer. When plasma generated, UV light and ROS(Reactive oxygen species), RNS(Reactive nitrogen species) can generated and those things effect to biological material. So we made simple plasma device using needle type of electrode and generated plasma. We used three kinds of gas and measured applied voltage and current. Also we observed optical emission spectrum. Using deuterium ramp, we can observed absorption spectrum and calculated radical density by lambert-beer's law. It is around ~1016cm3. And we can see the time-resolved absorption spectrum from monochromator, PMT(photo multiply tube), IV-converter, oscilloscope.

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Studies on isolation and identification of thiobacillus concretivorus (Thiobacillus concretivorus의 분리 및 동정에 관한 연구)

  • 하영칠;차승희;안승구
    • Korean Journal of Microbiology
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    • v.10 no.4
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    • pp.155-166
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    • 1972
  • With respect to a bacterium isolated some of the similarities and differences previously indicated between Thiobacillus thiooxidans and Thiobacillus concretivorus wee confirmed and the bacteria also provided considerable diferences compared to the two species in some repects. Severe precipitation of sulfur occurred in colonies and in liquid media of the organism isolated. The organism isolated utilized nitrate and asparagine as well as ammonium sulfate as a sole nitrogen source and grew well without being nearly inhibited by citrate and malate. This organism also showed the resistance to heats and external physical stimulations. Owing to some characters decribed above and the reports proposed, the organism isolated could be concluded to be Thiobacillus concretivorus and it was suggested that Thiobacillus concretivorus might be an apparently different species from Thiobacillus thiooxidans.

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Antibodies against Nitric Oxide Damaged Poly L-Tyrosine and 3-Nitrotyrosine Levels in Systemic Lupus Erythematosus

  • Khan, Fozia;Ali, Rashid
    • BMB Reports
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    • v.39 no.2
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    • pp.189-196
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    • 2006
  • Alterations in the amino acid structure or sequence can generate neo-epitopes from self-proteins causing autoaggressive immune attack. Reactive nitrogen species are an important factor that induces post-translational modification of proteins by cellular reduction and oxidation mechanism; cysteinyl-nitrosylation or tyrosine nitration leading to potentially pathogenic pathways. It was thought of interest to investigate the immunogenicity of nitrated poly L-tyrosine vis-$\`{a}$-vis its possible role in the induction of antibodies in systemic lupus erythematosus (SLE). Commercially available poly L-tyrosine was exposed to nitrating species and the damage was monitored by UV spectroscopy and alkaline gel electrophoresis. The results indicated the formation of 3-nitrotyrosine. Nitrated poly L-tyrosine induced higher titre antibodies as compared to the native form. Nitrated poly L-tyrosine was recognized by the autoantibodies present in the sera of patients suffering from SLE by enzyme immunoassays and band shift assay. The possible role of nitrated self-proteins has been discussed in the production of circulating anti-DNA antibodies in SLE.

Plasma and VUV Pretreatments of Polymer Surfaces for Adhesion Promotion of Electroless Ni or Cu Films

  • Romand, M.;Charbonnier, M.;Goepfert, Y.
    • Journal of Adhesion and Interface
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    • v.4 no.2
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    • pp.10-20
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    • 2003
  • This paper is relative to the electroless deposition of nickel or copper films on polyimide and polytetrafluoroethylene substrates. First, it is presented an original approach of the electroless process which consists in grafting nitrogenated functionalities on the polymer surfaces via plasma or VUV-assisted treatments operating in a nitrogen-based atmosphere ($NH_3$, $N_2$), and then in catalysing the grafted surfaces in an aqueous tin-free, Pd(+2)-based solution. Adhesion of the Pd(+2) catalytic species on polymer surfaces is explained by the formation of strong covalent bonds between these species and the grafted nitrogenated groups. Second, it is show how a fragmentation test performed in conjunction with electrical measurements can be used to characterize the practical adhesion of the electroless coatings deposited on flexible polymer substrates, and to evidence the influence of some experimental parameters (plasma treatment time and nature of the gas phase).

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EVALUATION OF THREE TROPICAL LEGUMES IN DIETS FOR GROWING RABBITS

  • Lowry, J.B.;Schlink, A.C.;Hoffmann, D.
    • Asian-Australasian Journal of Animal Sciences
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    • v.5 no.2
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    • pp.257-259
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    • 1992
  • Three tropical legumes, very different in growth form, but believed to be of potential value for animal production were evaluated by substituting the leaf meal made from each for lucerne at the level used in a standard diet for growing rabbits (50%). Each leaf meal had a nitrogen content of close to 3.5% and contributed about 60% of the crude protein in the diet. Albizia lebbeck and Clitoria ternatea showed no evidence of toxic or antinutrient effects. The protein digestibility of the complete diets were 66 and 61% respectively, implying a protein digestibility of the leaf of at least 50%. Both species would be suitable for practical production diets for rabbits and should be excellent for ruminants. In contrast, the diet containing Desmanthus virgatus had a protein digestibility of only 40%, implying that only about 15% of the leaf protein was available. The leaves showed marked non-enzymic browning on drying. When dried this species is clearly unsuitable as a feed for rabbits and possibly also for ruminants. However, it may well be valuable as fresh forage.

Antioxidant Activity of Fruits of Ligustrum japonicum (여정실의 항산화 활성)

  • Seo, Youngwan;Kim, Hojun
    • Ocean and Polar Research
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    • v.39 no.2
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    • pp.115-124
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    • 2017
  • The objective of this study is to evaluate the antioxidant activity of the fruits of Ligustrum japonicum. The crude extract was successively fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water fractions by means of solvent polarity. The crude extract and its solvent fractions were evaluated for their antioxidant effect by four different assay systems: scavenging power on peroxynitrite and intralcellular ROS produced in HT-1080 cells; DNA oxidation inhibition; ferric reducing antioxidant power (FRAP). The n-BuOH fraction exhibiting potent antioxidant activity was further purified by C18 silica gel column chromatography and RP-HPLC to give tyrosol (1) and salidroside (2). The structure of isolated compounds was determined by extensive 2 D NMR experiments such as $^1H$ COSY, NOESY, HSQC and HMBC as well as by comparison with the published spectral data.

Effects of Dietary Energy Level and Feeding Ration on Growth and Body Composition of Nile Tilapia, Oreochromis niloticus (L.)

  • Cho, Sung-Hwoan;Jo, Jae-Yoon
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2000.05a
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    • pp.271-273
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    • 2000
  • Recently, possibility of low protein diet with high energy level for improvement of fish production has been proposed in several fish species, especially under restricted feeding rate, fish responded better on high energy diet than low energy diet McGoogan and Gatlin (1999) stressed another benefit of low protein diet with high energy for fish production, in terms of reduction of nitrogen waste from fish metabolism However, others reported no effect of high lipid in feed on performance of fish (Jover et al. 1999). This was probably because that energy availability o requirement for fish varied based on fish species, protein content or quality in feed, rearing water temperature condition and feeding ration. (omitted)

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Photocatalytic oxidation reaction in removal of NH4-N by using TiO2 (TiO2를 이용한 암모니아성 질소 제거에 관한 광촉매 산화반응)

  • 박상원;김정배
    • Journal of Environmental Science International
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    • v.12 no.10
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    • pp.1071-1077
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    • 2003
  • The aim of this study is, firstly, to find out what kinds of inorganic species are produced in the photocatalytic oxidation of ammonium-nitrogen containing water and, secondly, to seek the influence of anion for the photocatalytic oxidation of ammonium contained compounds. The photoenergy above 3 eV(λ <415 nm) was effectively absorbed by TiO$_2$ and TiO$_2$/polymer was used to be oxidized NH$_4$-N in wastewater to NO$_3$-N. Existing the anion as Cl$\^$-/, the rate of photocatalytic oxidation decreased regardless of other condition. This result showed that the chloride ions reduced the rate of oxidation by scavenging oxidizing radical species as OH$\^$-/ and OCl$\^$-/. Some of the added ion might have blocked the active sites of the catalyst surface, thus deactivated the catalyst.

The Standing Crop and Edaphic Factor of Grasslands in Chuiwon near the D.M.Z. (철원지구 비무장지대 초지의 지력에 Standing Crop의 변동에 관한 연구)

  • 이정혜;장남기
    • Asian Journal of Turfgrass Science
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    • v.8 no.1
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    • pp.37-46
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    • 1994
  • We study on the standing crop and edaphic factor of grasslands in Chulwon near the D.M.Z. The widespread and most abundant grasses are Arundinella hirta and Miscanthus sinensis in the upland fields of D.M.Z. The vascular flora of grasslands in Umi-dong and Sukda-dong near the D.M.Z. and composed of total 25 species, the most important of which ace Arundinella hirta and Miscanthus sinensis These two species contribute greatly to the standing crop of live naterial was in excess of $107.6g /m^2$. Correlation between standing ccop and water content, organic matter, total nitrogen, or avail-able phosphorus of grassland soils in area studied is high significant. It is argued that these edaphic factors affect the growth of grasses in Umi-dong and Sukda-dong grasslands.

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Bacterial endophytes from ginseng and their biotechnological application

  • Chu, Luan Luong;Bae, Hanhong
    • Journal of Ginseng Research
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    • v.46 no.1
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    • pp.1-10
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    • 2022
  • Ginseng has been well-known as a medicinal plant for thousands of years. Bacterial endophytes ubiquitously colonize the inside tissues of ginseng without any disease symptoms. The identification of bacterial endophytes is conducted through either the internal transcribed spacer region combined with ribosomal sequences or metagenomics. Bacterial endophyte communities differ in their diversity and composition profile, depending on the geographical location, cultivation condition, and tissue, age, and species of ginseng. Bacterial endophytes have a significant effect on the growth of ginseng through indole-3-acetic acid (IAA) and siderophore production, phosphate solubilization, and nitrogen fixation. Moreover, bacterial endophytes can protect ginseng by acting as biocontrol agents. Interestingly, bacterial endophytes isolated from Panax species have the potential to produce ginsenosides and bioactive metabolites, which can be used in the production of food and medicine. The ability of bacterial endophytes to transform major ginsenosides into minor ginsenosides using β-glucosidase is gaining increasing attention as a promising biotechnology. Recently, metabolic engineering has accelerated the possibilities for potential applications of bacterial endophytes in producing beneficial secondary metabolites.