Ocean and Polar Research

Korea Institute of Ocean Science & Technology (한국해양과학기술원)
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Antioxidant Activity of Fruits of Ligustrum japonicum

여정실의 항산화 활성

  • Seo, Youngwan (Division of Marine Bioscience, Korea Maritime and Ocean University) ;
  • Kim, Hojun (Division of Marine Bioscience, Korea Maritime and Ocean University)
  • 서영완 (한국해양대학교 해양생명과학부) ;
  • 김호준 (한국해양대학교 해양생명과학부)
  • Received : 2017.03.21
  • Accepted : 2017.04.15
  • Published : 2017.06.30
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Abstract

The objective of this study is to evaluate the antioxidant activity of the fruits of Ligustrum japonicum. The crude extract was successively fractionated into n-hexane, 85% aqueous methanol (85% aq.MeOH), n-butanol (n-BuOH), and water fractions by means of solvent polarity. The crude extract and its solvent fractions were evaluated for their antioxidant effect by four different assay systems: scavenging power on peroxynitrite and intralcellular ROS produced in HT-1080 cells; DNA oxidation inhibition; ferric reducing antioxidant power (FRAP). The n-BuOH fraction exhibiting potent antioxidant activity was further purified by C18 silica gel column chromatography and RP-HPLC to give tyrosol (1) and salidroside (2). The structure of isolated compounds was determined by extensive 2 D NMR experiments such as $^1H$ COSY, NOESY, HSQC and HMBC as well as by comparison with the published spectral data.

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References

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