• 한국작물학회:학술대회논문집
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• 한국작물학회 2022년도 추계학술대회
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• pp.106-106
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• 2022

An appropriate amount of nitrogen fertilizer input during rice cultivation is essential for rice growth, quality control, and reduction of greenhouse gases in paddy fields. Therefore, it is necessary to develop a technology that can check whether an appropriate amount of fertilizer is applied in paddy fields. In this study, we tried to derive a method for diagnosing nitrogen fertilization level using spectroscopic diagnosis, physiological analysis, and molecular indicator genes. Nitrogen fertilization treatment was performed in a greenhouse by dividing into five treatment conditions: no fertilization (N0), low fertilization (N0.5), standard fertilization (N1.0), excessive fertilization (N1.5), and double fertilization (N2.0), respectively. Growth characteristics analysis was investigated by nitrogen fertilization conditions and growth stages, and the height of the canopy was analyzed using a laser scanner. Physiological and spectroscopic analyses were performed by analyzing chlorophyll and sugar contents and measuring SPAD and leaf spectrometer on rice leaves. In addition, real-time PCR experiment was performed to check the relative expression levels of several known nitrogen metabolism related genes. These results suggest that spectroscopic techniques can be helpful in diagnosing the level of nitrogen fertilization in rice paddy fields.

• 한국환경농학회지
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• 제42권1호
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• pp.28-34
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• 2023

Nitrogen fertilizers applied to agricultural lands for crop cultivation can be volatilized as ammonia. The released ammonia can catalyze the formation of ultrafine dust (particulate matter, PM2.5), classified as a short-lived climate change pollutant, in the atmosphere. Currently, one of the prominent methods for fertilizer application in agricultural lands is soil surface application, which comprises spraying the fertilizers onto the soil surface, followed by mixing the fertilizers with the soil. Owing to the low nitrogen absorption rate of crops, when nitrogen fertilizers are applied in this manner, they can be lost from land surfaces through volatilization. Therefore, investigating a new fertilization method to reduce ammonia emissions and increase the fertilizer utilization efficiency of crops is necessary. In this study, to develop a method for reducing ammonia emissions from nitrogen fertilizers applied to soil surfaces, deep fertilization was conducted using a newly developed deep fertilization device, and ammonia emissions from barley, garlic, and onion fields were examined. Conventional fertilization (surface application) and deep fertilization (soil depth of 25 cm) were conducted for analysis. The fertilization rate was 100% of the standard fertilization rate used for barley, and deep fertilization of N, P, and K fertilizers was implemented. Ammonia emissions were collected using a wind tunnel chamber, and quantified subsequently susing the indole-phenol blue method. Ammonia emissions released from the basal fertilizer application persisted for approximately 58 d, beginning from approximately 3 d after fertilization in conventional treatments; however, ammonia was not released from deep fertilization. Moreover, barley, garlic, and onion yields were higher in the deep fertilization treatment than in the conventional fertilization treatment. In conclusion, a new fertilization method was identified as an alternative to the current approach of spraying fertilizers on the soil surface. This new method, which involves injecting nitrogen fertilizers at a soil depth of 25 cm, has the potential to reduce ammonia emissions and increase the yields of barley, garlic, and onion.

• 한국근적외분광분석학회:학술대회논문집
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• 한국근적외분광분석학회 2001년도 NIR-2001
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• pp.1262-1262
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• 2001

The rice plant is one of the important staple crops in Korea. The high yield with low cost in rice is required the soil fertility and the development of new precise method of fertilizer application by nutritional diagnosis. Now, in Korea, the nitrogen application system for the rice plant is composed of the basal fertilization, fertilization at tillering stage and fertilization at panicle stage, which the nitrogen fertilization at panicle stage amount to about 30 percent in the total amount. Thus, this experiment carried out to the development of the system that can measure the nitrogen content in the rice plant at panicle stage rapidly with the near infrared spectroscopy, and to predict the appropriate quantity of the nitrogen fertilization at panicle stage based on calibration model for test of nitrogen content in rice plant. The samples were collected from 48 varieties in 4 regions which are mainly cultivated in the southern part of Korea. And then, it collected by classifying into the leaf, the whole plant and the stem since 7 days before the nitrogen fertilization at panicle stage. The ranges of the nitrogen contents were 1.6∼4.0%, 1.7∼3.0% and 1.4∼2.7% in the leaf, the whole plant and the stem, respectively. In the calibration models created by each part of the plant under the Multiple Linear Regression(MLR) method, the calibration model for the leaf recorded the relatively high accuracy. The mutual crossing test on unknown samples were carried out using Partial Least Square(PLS) calibration model. That is, the nitrogen content in the stem was tested by calibration model made by the leaf model and that of stem was tested by calibration model made by whole plant sample. When unknown leaf sample was tested by calibration model made by all sample that collected from each part in rice plant such as leaf, stem and whole plant, it recorded the highest accuracy. As a result, to test the nitrogen content in the rice plant at panicle stage, the nitrogen content in the leaf shall be tested by the calibration model composed of the leaf, the stem and the whole plant. In future, to estimated the amount of nitrogen fertilization at panicle stage for rice plant , it will be calculated based on regression model between rice yield and nitrogen content of leaf measured by calibration model made by mixed sample including leaf, stem and whole plant.

• 한국초지조사료학회지
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• 제18권2호
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• pp.113-122
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• 1998

This experiment was conducted to establish the cultural method of triticale(Triticum Secalotriticum Saratoviense Meister) as a whole crop silage by evaluating the effect of seeding date and nitrogen fertilization rate on forage yields and feeding value. Heading date, flowering date, and the dough stage of development came significantly earlier as triticale was seeded earlier. Soilage, dry matter yields and percent dry matter significantly varied with seeding dates and crude ash, NFE and TDN. However, TDN yield was significantly reduced by delayed seeding. Macromineral contents were not affected by seeding date. Soilage, dry matter yield, and percent dry matter significantly increased as nitrogen fertilization rate increased. The contents of crude protein, crude fat, crude ash, NFE, TDN and TDN yield also significantly increased. In contrast, crude fiber content of triticale decreased with increasing N fertilization rate.

• 한국토양비료학회지
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• 제39권2호
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• pp.95-101
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• 2006

밭작물의 시비효율 증대 및 생력시비기술 확립을 위하여 토중시비기를 개발하고 비닐피복 후 배추 가락신 1호를 재배하여 시비간격 30 cm, 시비 깊이 15 cm로 토중시비시 토양화학성 변화, 양분흡수량 및 이용률, 작물의 생육과 수량성을 검토한 결과는 다음과 같다. 토양 중 질산태질소 함량은 생육초기에서 중기까지 토중시비구에서 질소 시비수준이 높을수록 증가하였고 시험 후 토양은 토중시비에서 총질소 함량은 토중시비에서 증가한 반면 유기물, 유효인산 및 치환성 칼륨 함량은 비슷하였다. 배추의 생장속도는 파종 후 65일에 토중시비 100% 구, 70% 구에서 높게 증가되었고 시비질소 흡수량은 토중시비에서 질소 시비수준이 높을수록 많았고 질소 이용률은 관행시비(33.5%)에 비하여 토중시비에서 15-21% 높았다. 토중시비 70%구와 100%구는 질소시비효율을 증대 시켰을 뿐만 아니라 수량도 관행시비($109Mg\;ha^{-1}$)에 비하여 16-20% 증수되었고 소득이 18-22%나 증가되었다. 결론적으로 질소비료를 30% 절감한 토중시비를 추천할 수 있는 시비방법이라고 판단되었다.

• 한국작물학회지
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• 제50권1호
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• pp.16-21
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• 2005

This study was conducted to establish the elaborate nitrogen fertilization method to enhance N use efficiency in direct-seeded rice on flooded paddy. The nitrogen uptake by rice plants was insignificant until 25 days after seeding, and increased gradually thereafter. During this early growth stage, rice plants absorbed only the $4\%$ of basal applied N, while the $45\%$ of N fertilizer remained in the paddy soil. The absorption of basal N by rice plants was almost completed at 46 days after application. Nitrogen top-dressed at 5-leaf stage was well matched to crop nutrient demand, so it could be absorbed so actively in 8days after application. As a result, we could cut down the amount of N fertilizer to $36\%$ of the basal N level without significant difference in yield. Plant recoveries of fertilizer $^{15}N$ applied with different application timings were $7.8\%$ for basal, $9.4\%$ for 5-leaf stage, $17.1\%$ for tillering stage, and $23.4\%$ for panicle initiation stage, respectively. When urea was applied with nitrogen fertilization practice based on basal incorporation (BN), plant recovery of $^{15}N$ at harvest was $31.0\%$, which was originated from $13.7\%$ for grain, and $21.3\%$ of the fertilizer $^{15}N$ remained in the soil, and the rest could be uncounted. Plant recovery of fertilizer $^{15}N$ applied with nitrogen fertilization practice based on topdressing at 5-leaf stage (TN), where N rate was reduced by $18\%$ compared with BN, was $35.1\%$ (grain $15.6\%$), and $19.9\%$ of the fertilizer $^{15}N$ remained in the soil, and the rest could be uncounted. TN showed a higher $^{15}N$ recovery than BN because it was to apply N fertilizer at a time to well meet the demand of rice plant direct-seeded on flooded paddy. We concluded that TN would be the nitrogen fertilization method to enhance N use efficiency in direct-seeded rice on flooded paddy.

• 한국환경농학회지
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• 제41권4호
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• pp.230-235
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• 2022

BACKGROUND: Ammonia gas emitted from nitrogen fertilizers applied in agricultural land is an environmental pollutant that catalyzes the formation of fine particulate matter (PM2.5). A significant portion (12-18%) of nitrogen fertilizer input for crop cultivation is emitted to the atmosphere as ammonia gas, a loss form of nitrogen fertilizer in agricultural land. The widely practiced method for fertilizer use in agricultural fields involves spraying the fertilizers on the surface of farmlands and mixing those with the soils through such means as rotary work. To test the potential reduction of ammonia emission by nitrogen fertilizers from the soil surface, we have added N, P, and K at 2 g each to the glass greenhouse soil, and the ammonia emission was analyzed. METHODS AND RESULTS: The treatment consisted of non-fertilization, surface spray (conventional fertilization), and soil depth spray at 10, 15, 20, 25, and 30 cm. Ammonia was collected using a self-manufactured vertical wind tunnel chamber, and it was quantified by the indophenol-blue method. As a result of analyzing ammonia emission after fertilizer treatments by soil depth, ammonia was emitted by the surface spray treatment immediately after spraying the fertilizer in the paddy soil, with no ammonia emission occurring at a soil depth of 10 cm to 30 cm. In the upland soil, ammonia was emitted by the surface spray treatment after 2 days of treatment, and there was no ammonia emission at a soil depth of 15 cm to 30 cm. Lettuce and Chinese cabbage treated with fertilizer at depths of 20 cm and 30 cm showed increases of fresh weight and nutrient and potassium contents. CONCLUSION(S): In conclusion, rather than the current fertilization method of spraying and mixing the fertilizers on the soil surface, deep placement of the nitrogen fertilizer in the soil at 10 cm or more in paddy fields and 15 cm or more in upland fields was considered as a better fertilization method to reduce ammonia emission.

• The Korean Journal of Ecology
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• 제26권2호
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• pp.59-64
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• 2003

We examined the effects of fertilization [control (C), 200 kg N ha^{-1} + 25 kg P ha^{-1}$ (LNP), and 400 kg N $ha^{-1} + 50 kg P ha^{-1}$ (HNP)] on fine root (＜ 2 mm diameter) dynamics using monthly soil coring method in a 39-year-old Pinus rigida plantation of central Korea. The average fine root biomass (live + dead) (kg $ha^{-1}$ $\pm$ SE) during the first growing season for C, LNP, and HNP was 1301 $\pm$ 54, 1084 $\pm$ 47, and 1328 $\pm$ 22, respectively. The fine root production (kg $ha^{-1}$ $\pm$ SE) was 2394 $\pm$ 128 for C, 2048 $\pm$ 101 for LNP, and 2768 $\pm$ 150 for HNP, respectively. Over the same period, fertilization treatments had impact on N and P concentrations of live fine root. Nitrogen and P inputs (kg $ha^{-1}$ $yr^{-1}$) into the soil through fine root turnover for C, LNP, and HNP were 16.6 and 0.9, 17.2 and 0.9, and 24.1 and 1.6, respectively. There were no significant differences in fine root biomass and production during the first growing season after fertilization. However, fertilization increased fine root N and P concentrations, and in consequence resulted in increased N and P inputs into soil through fine root turnover.

• 한국환경농학회지
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• 제42권1호
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• pp.44-51
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• 2023

Nitrogen fertilizer is an essential macronutrient that requires repeated input for crop cultivation. Excessive use of nitrogen fertilizers can adversely affect the environment by discharging NH₃, NO, and N₂O into the air and leaching into surrounding water systems through rainfall runoff. Therefore, it is necessary to develop a technology that reduces the amount of nitrogen fertilizer used without compromising crop yields. Fertilizer deep placement could be a technology employed to increase the efficiency of nitrogen fertilizer use. In this study, a deep fertilization device that can be coupled to a tractor and used to inject fertilizer into the soil was developed. The deep fertilization device consisted of a tractor attachment part, fertilizer amount control and supply part, and an underground fertilizer input part. The fertilization depth was designed to be adjustable from the soil surface down to a depth of 40 cm in the soil. This device injected fertilizer at a speed of 2,000 m²/hr to a depth of 25 to 30 cm through an underground fertilizer injection pipe while being attached to and towed by a 62-horsepower agricultural tractor. Furthermore, it had no difficulty in employing various fertilizers currently utilized in agricultural fields, and it operated well. It could also perform fertilization and plowing work, thereby further simplifying agricultural labor. In this study, a newly developed device was used to investigate the effects of deep fertilizer placement (FDP) compared to those with urea surface broadcasting, in terms of rice and soybean grain yields. FDP increased the number of rice grains, resulting in an average improvement of 9% in rice yields across three regions. It also increased the number of soybean pods, resulting in an average increase of 23% in soybean yields across the three regions. The results of this study suggest that the newly developed deep fertilization device can efficiently and rapidly inject fertilizer into the soil at depths of 25 to 30 cm. This fertilizer deep placement strategy will be an effective fertilizer application method used to increase rice and soybean yields, in addition to reducing nitrogen fertilizer use, under conventional rice and soybean cultivation conditions.

• 한국토양비료학회지
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• 제39권5호
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• pp.298-302
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• 2006

비닐피복작물 재배시 시비효율 증대 및 생력시비기술 확립을 위하여 파종과 동시에 시비가 가능한 토중시비기를 개발하였고 개발된 파종기와 시비기를 이용하여 1999년부터 2000년까지 시비간격 30 cm, 시비깊이 15 cm, 파종간격 25 cm로 배수가 양호한 사양토에서 땅콩을 재배한 후 작업정도, 토양화학성, 양분흡수량 및 수량성에 미치는 영향을 평가하였다. 땅콩의 파종 소요시간은 인력($173hr\;ha^{-1}$)에 비하여 90% 이상 노력절감 효과가 있었으나 출현일수는 4일정도 지연되었으며 입모율은 관행구에 비하여 11~18% 낮았다. 시험 전 토양에 비하여 시험 후 토양의 총질소 함량은 증가한 반면에 유기물, 유효인산 및 치환성 칼륨함량은 감소되었으며 토양의 질산태질소 합량은 생육초기에서 중기까지 국소시비구에서 시비량이 많을수록 증가하였다. 땅콩의 질소흡수량은 근류균에 의한 자체 고정량이 많아 시비방법 및 시비량별 큰 차이가 없었으나 인산 및 칼리흡수량은 국소시비 70%, 100%구에서 높은 경향을 나타냈다. 땅콩의 생장속도는 시비방법별 공히 파종 후 120일에 가장 많이 증가되었고 관행시비구와 국소시비 70%구에서 생육후기까지 증가폭이 크게 나타났으며 국소시비 70%구는 협수가 많고 협실비율 및 완숙립중 비율이 높아 종실중이 관행시비구 $3,150kg\;ha^{-1}$에 비하여 4% 증수되었다.