• 환경생물
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• 제37권4호
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• pp.554-567
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• 2019

최근 농경지 투입양분으로 인한 환경영향의 우려와 함께 하천 등으로 유출되는 양분의 오염부하 관리가 요구되고 있다. 이에 정부는 국내 농지의 양분 과잉 투입 문제를 해결하기 위해 농경지의 양분관리 목표를 설정하여 관리하는 양분관리제도의 도입을 추진하고 있다. 본 연구는 국내 합리적인 양분관리제도 도입을 위한 양분관리 지표 기준을 설정하고자 도 단위의 양분부하와 양분수지 지표를 분석하고 지표별 특성을 비교 검토하였다. 도 단위 질소와 인성분의 양분부하와 양분수지 분석에서 지역별 양분부하 순위와 양분수지 순위가 다르게 나타나, 지역의 양분수준을 평가하는데 있어 양분부하와 양분수지의 두 지표는 서로 상이한 지표 값을 도출하는 것으로 나타났다. 양분부하와 양분수지 간의 상관관계 분석에서 질소성분의 경우 상관관계 계수(r)는 0.2504로서 5% 유의수준에서 통계적 유의성이 없었으며, 인성분의 경우 상관관계 계수(r)가 0.7375로서 5% 유의수준에서 통계적 유의성이 인정되었다. 따라서 양분관리 지표활용에 있어 인성분의 경우 양분부하와 양분수지 간의 상호 호환성이 나타났으나, 질소 성분의 경우 양분부하와 양분수지 간의 상호 호환성이 없는 것으로 나타났다. 따라서 지역의 양분관리 지표로서 농업환경 특성을 반영하는 양분수지 지표의 활용이 합리적인 것으로 평가되었다.

• 동아시아식생활학회지
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• 제7권1호
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• pp.13-20
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• 1997

This study was designed to determine the effects of protein sources on the curation of gastric ulceration, protein metabolism, and nitrogen balance in rats with gastric ulcer induce by restraint and water immersion stress. After the rats were fed 10% casein diet for 3 weeks, four groups of the rats were forced in 5$\times$5$\times$15cm plexiglass cage. The restraint and water immersion stress was carried at 20$\pm$2$^{\circ}C$ for 8-hour. The other one group(control group) was not exposed to stress. After stress 4 kinds of different diets containing 20% protein were given for 5 days. The protein sources were casein, whey protein, soy protein, gluten. The control group was fed to 10% casein diet. The results were as follows ; the weights of rats were not different among the diet groups During the experiment period follows ; the weights of rats were not different among the diet groups during the experiment period (for 5 days). The ulcer index of rats fed 10% gluten and soy protein diet was significantly higher than those of casein and whey protein diet groups(p<0.05). The level of serum albumin was not significantly different among diet groups. But hematocrit and the level of $\alpha$-amino-N, BU and UUN of plant protein diet groups were higher than animal diet groups, the urinary hydroxyproline of soy protein group was the highest and the whey protein was the lowest. The digestibility and BV of nitrogen of gluten diet group were significantly higher than those of casein and whey protein diet groups(p<0.05). The animal proteins had more curative effects of ulcer than plant animals. The results of this study provide useful information concerning diet therapy for the patients with gastrointestinal diseases and the field of enteral diet materials.

• 한국가금학회지
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• 제38권1호
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• pp.37-43
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• 2011

본 연구는 맹장 결찰계를 이용하여서 [15N]urea의 이용성을 조사하였다. 질소의 balance 및 이용률은 맹장 결찰 수술에 의해서 높아지는 경향이 인정되었다. 이는 맹장 결찰 수술로 인한 요산 배설량의 유의한 감소로 인한 결과였다(사료 및 요소 유래 질소 이용률, P<0.01). 닭에 있어서 맹장 결찰 수술은 사료 유래 ammonia 배설량은 증가(P<0.01), 요소 유래 ammonia배설량은 감소(P<0.01)시켰다. 요소 유래, 요소질소의 배설량은 맹장 결찰 수술에 의해서 유의하게 증가하였지만(P<0.01), 맹장 결찰계도 5% 단백질 사료와 같이 급여한 요소를 51.6%정도 이용하는 것으로 나타났다. 결론적으로 닭에 있어서 단백질 수준이 결핍된 사료와 같이 급여된 요소의 이용에 있어서 맹장은 유의한 역할을 한다고 할 수 있다. 하지만 질소의 이용에 있어서는 반드시 긍정적인 역할만을 한다고는 결론내릴 수 없다.

• 농업과학연구
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• 제42권4호
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• pp.431-437
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• 2015

자원순환 및 친환경 농업을 실천하는 농가가 증가함에 따라 유기물을 활용한 농경지 양분관리에 대한 필요성이 대두되고 있다. 질소 공급원으로 유기물을 시용했을 때 질소 공급효과에 대한 연구들을 정리한 결과는 다음과 같다. 농경지에 유기물을 시용했을 때 질소의 공급은 질소 순환에서 무기화 과정을 통해 이루어지기 때문에 작물의 단기적인 질소 이용률은 무기질 비료에 비해 낮다. 유기물의 질소 공급량은 유기물의 C/N율이 낮을수록 무기화율이 높아지므로 C/N율을 고려한 유기물 시용이 중요하다. 가축분 퇴비의 경우 축종별 분뇨 질소함량과 퇴비와 혼합된 부산물 종류에 따라 질소 무기화율이 달라질 수 있으며, 녹비는 작물 종류와 환원시기, 토양 양분조건 등에 따라 C/N율이 달라진다. 또한 적정량의 질소를 추천하기 위해서는 토양 중 유기태 질소로부터 가용화 될 수 있는 질소의 양을 측정하여야 한다. 지금까지 보고된 퇴비, 녹비에 대한 질소공급과 작물 생산성에 대한 연구결과는 재배 여건과 작물 종류 등에 따라 다르게 나타나 질소 공급을 위한 유기물 추천 모델을 구성하기 위한 기초자료로 활용하기에는 어려움이 있다. 유기물 추천 모델을 구성하기 위해서는 우선적으로 유기물질 종류별 질소 공급량에 대한 기초 데이터가 구축되어야 하며, 토양의 질소공급량을 평가할 수 있는 분석 방법의 구축이 필요하다.

• 한국신재생에너지학회:학술대회논문집
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• 한국신재생에너지학회 2009년도 춘계학술대회 논문집
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• pp.430-433
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• 2009

The development of large-scale environmental friendly livestock complex in the reclaimed tideland is one of different alternatives to increase the competitiveness of internal livestock industry against an international opening markets as DDA and FTA in agricultural field. Recently, it is possible to introduce an environmental friendly livestock complex in the reclaimed tideland by an amendment of the acts for agricultural land. However more studies that are on the basis of nitrogen and phosphorus mass balance need to preserve the agricultural environments as the quality of agricultural water and soil in rural area. In this study, the reference for feasibility study is Whaong reclaimed tideland which located at Whaseong city, Gyeonggi Province, and a basic concept of environmental friendly livestock complex is the production of forage crops with the supply of liquid fertilizer and the production of bioenergy such as biogas by the recycling of pig slurry as a resource. The mass balance of nitrogen based on between forage crops such as maize, barley and liquid fertilizer supplied at the reclaimed tideland, and also it was estimated an economical efficiency as anaerobic digestion plant for treating pig slurry of $100m^3/day$ introduce in an environmental friendly livestock complex.

• Asian-Australasian Journal of Animal Sciences
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• 제21권9호
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• pp.1303-1311
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• 2008

Twenty four early lactating Nili-Ravi buffaloes, eight in each group, were used in a Randomized Complete Block Design to evaluate the influence of varying ruminally degradable protein (RDP) to ruminally undegradable protein (RUP) ratio on feed intake, digestibility, N balance, milk yield and its composition, conception rate and days open. Three experimental diets were formulated to contain RDP:RUP of 50:50, 66:34 and 82:18 and were denoted as HRUP, MRUP and LRUP, respectively. Dry matter (DM) intake was higher (p<0.05) in buffaloes fed HRUP diet than in those fed MRUP and LRUP diets. Dry matter digestibility was higher (p<0.05) in buffaloes fed LRUP diet than in those fed HRUP and MRUP diets. Linear increase was observed in DM digestibility with increasing RDP:RUP while Neutral detergent fiber digestibility remained unaltered in buffaloes fed HRUP and MRUP diets, however, it was higher than in those fed LRUP diet. Crude protein digestibility remained unaltered across all treatments. Milk and 4 percent fat corrected milk (4% FCM) yield was higher (p<0.05) in buffaloes fed HRUP diet than those fed MRUP and LRUP diets. Linear decrease in milk yield was observed with increased RDP:RUP. Milk protein and fat yields were higher (p<0.05) in animals fed HRUP diet than those fed MRUP and LRUP diets. Milk protein percent in animals fed HRUP diet was higher than in those fed LRUP diet, whereas it did not differ with those fed MRUP diet. Percent of fat, total solids, solid not fat and lactose remained unaltered across all diets. Nitrogen balance was higher in buffaloes fed HRUP diet than in those fed other diets. Increasing the RDP:RUP resulted in a linear decrease in N balance. The blood urea nitrogen and milk urea nitrogen were lower (p<0.05) in buffaloes fed HRUP diet than those fed MRUP and LRUP diets. The blood pH remained unaltered across all treatments. Days open did not differ significantly. Conception rate was higher in buffaloes fed HRUP diet than those fed MRUP and LRUP diets. The findings of the present study indicate that feeding high (50% of the total crude protein) ruminally undegradable protein diet not only increased nutrient intake and milk yield but also improved conception rate in early lactating buffaloes.

• Journal of Nutrition and Health
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• 제20권2호
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• pp.90-103
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• 1987

A study was conducted to measure nitrogen intake and excretion in young korean women on their normal diet and leading normal activity level. Dietary survey by food record, fecal and urinary samples were collected for 3 days in 19 healthy female college students in Korea. On one of the 3 days, duplicate of one-day diet was collected from each subjects. Mean daily intakes of energy and protein were calculated from food recorded. Duplicate diet samples and pooled fecal samples were analyzed for N content. Pooled urine samples were analyzed for total N, urea N, and creatinine content. Apparent N absorption, apparent N balance and urea N/creatinine N were calculated to evaluate protein nutritional status. The results obtained are summarized as following ; 1) Accordingly to food record, mean daily intakes of energy, protein, carbohydrate and far were 1535.2 $\pm$53.78Kcal, 55.95$\pm$2.97g(total nitrogen 8.95$\pm$0.45g), 254.13$\pm$10.31g and 39.24$\pm$2.76 g, providing 14.6%, 66.2%, 19.2% of total energy respectively. 2) Nitrogen intake by chemical analysis was 7.16$\pm$0.31g/day (protein 44.75$\pm$1.94kg/day) providing 82.39$\pm$4.58% of nitrogen intake by food record. The difference of total nitrogen intake between food record and chemical analysis in diets was significant(p<0.05). 3) Mean daily fecal nitrogen excretion was 1.38$\pm$0.10g and then mean apparent digesbility of protein was 80.53$\pm$5.21%. 4) Mean daily urinary nitrogen excretion, urea N excretion and creatinine excretion were 6.03$\pm$0.30g, 4.52$\pm$0.22g, and 0.88$\pm$0.04g respectively. Urinary urea nitrogen was 75.2$\pm$1.38% of total urinary nitrogen excretion and urinary nitrogen was 85.4$\pm$3.56% of total nitrogen intake by chemical analysis. 5) Mean urea N/creatinine N ration was 14.01$\pm$0.77. 6) Mean nitrogen balance was -0.244$\pm$0.33g/day. From the above results, it is concluded that the subjects in this study seem to be in marginal protein nutritional status and therefore should increase dietary protein intake.

• 한국식품영양과학회지
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• 제1권1호
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• pp.5-8
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• 1972

Ordinary biological methods of evaluating protein quality are subjected to various sources of error, such as preliminary feeding, body weight of the animals, length of feeding period, endogenous nitrogen metabolism, etc. (1). Wide variations in the reported data may be partly due to this fact. The author postulated that by applying labelled protein these shortcomings may be avoided and that a balance study using nitrogen-15 would give most reliable data, uninfluenced by other factors which are unavoidable in ordinary balance studies using unlabelled nitrogen (2). However, the most important prerequisite for testing this assumption is to obtain labelled protein. As it has been known long since that chlorella contains a large amount of protein and can be easily cultured in quantity (3, 4), it was thought that labelled protein might be obtained by adding $N^{15}$ into the culture media. In this study it has been tried to find out, therefore, whether it is possible to obtain labelled protein by incorporating $KN^{15}\;O_3$ into the culture fuid and at what stage of growth the incorporation of the label into chlorella occurs.

• Asian-Australasian Journal of Animal Sciences
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• 제15권10호
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• pp.1433-1438
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• 2002

The study was carried out to evaluate leucaena seeds as a protein replacement of mustard seed cake (MSC) in the concentrate mixture of growing lambs. Fifteen owing male lambs (Local${\times}$Corridale) with an average body weight of 16.3 kg were allocated into three dietary treatments (T1, T2, and T3) with five animals in each group. Animals were offered dry mixed grass, berseem hay and concentrate mixture to meet their nutrient requirements. In concentrate mixture of T1, (Control) MSC was used as protein source, while in T2 and T3 groups, 25 and 50% of MSC was replaced by leucaena leucocephala seeds. On completion of three months (90 days) of feeding, a digestion cum-metabolism trial was conducted to determine DMI, nutrient utilization, and nitrogen balance. Changes in body weight were recorded at 15 day internals and eating patterns were recorded for 3 consecutive days at the end of the feeding trial. MSC had higher CP contents than leucaena seeds (27.0%). Mimosine contents in leucaena seeds were 1.1 compared to 0.2 and 0.4% in concentrate mixture of T2 and T3 group, respectively. Dry matter intake varied non-significantly ($79.3{\pm}1.2$ to $83.4{\pm}1.3g/kg$ $w^{0.75}$) across the dietary treatments. Digestibility of DM and cell wall polysaccharides (NDF, ADF. Cellulose and hemicellulose) were comparable, however CP digestibility was relatively lower in leucaena luecocephala seeds based groups (T2 $45.5{\pm}1.7$ and T3 $46.7{\pm}3.5$) compared to MSC supplemented group (T1 $47.7{\pm}0.9%$). The growth rate of lambs was non-significantly higher in T1 ($79.2{\pm}5.4$) compared to T2 ($73.8{\pm}8.8$) and T3 ($73.9{\pm}7.0$), respectively. The animals were in positive nitrogen balance and N-balance varied from 1.8 to 2.9 g/d across treatment groups. The eating rate (% of total offered) of concentrate up-to 15 min was relatively higher in T1 (82.4) than T2 (74.2) and T3 (77.8%). However no effect of leucaena seeds was recorded on total DMI of animals. The results of the study revealed that the inclusion of up to 50% leucaena seeds, as protein source in concentrate mixture of lambs had no adverse effect on DMI, nutrient utilization, eating patterns, nitrogen balance and growth performance of lambs.

• Asian-Australasian Journal of Animal Sciences
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• 제15권7호
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• pp.992-997
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• 2002

Two in vivo experiments were conducted to evaluate the effect of novel urease inhibitor hydroquinone (HQ) on ammonia release rate from urea hydrolysis, nitrogen balance, nutrient digestibility and efficiency of microbial protein synthesis. In Exp. 1, twelve crossbred cannulated lambs were randomly assigned within initial body weight block to one of four HQ treatments, which included 0 (control), 30, 60 or 80 mg HQ/kg DM intake. Ammonia concentration and pH of ruminal fluid were immediately measured at 0, 2, 4, 6 and 8 h after feeding. Increasing the dose of HQ tended (p<0.15) to linearly decrease NH3 formation. The ammonia peak concentration (2 h post-feeding) in animals receiving HQ was approximately one-half of that in animals not receiving HQ (p<0.01), and a relatively sustained ammonia release could be obtained at the dose of 30 or 60 mg HQ/kg DM. In Exp. 2, sixteen intact crossbred lambs (weight $40{\pm}0.8kg$) were used in a $2{\times}2$ factorial design experiment. The four rations consisting of soybean meal-based (SBM) or urea-based (Urea) nitrogen source with or without HQ (S1, S0, U1 and U0) were fed in digestion and N balance trials. Apparent digestibility of major nutrients except that of ADF was not affected by either nitrogen source or addition of HQ. Regardless of nitrogen source, supplementation of HQ significantly improved ADF digestibility (p<0.05). The various ration had no effects on N metabolism in the presence of HQ. There was significant difference between total purine derivatives (PD), estimated efficiency of microbial N synthesis (p<0.05) and urea-N excretion (p<0.01) in the urine for the SBM ration and for the Urea ration. However, HQ had little influence on efficiency of microbial N synthesis as proportion of daily intake of total tract digestible OM (p>0.05). No interactions between main nitrogen source and HQ were measured throughout the trial. Results of this study suggest that addition of HQ to ration may improve ADF digestion with having no negative effect on N metabolism and microbial protein production.