DOI QR코드

DOI QR Code

맹장 결찰계(Cecal-ligated Chicken)를 이용한 [15N]urea의 이용성에 관한 연구

A Study on the Utilization of Dietary [15N]urea in Cecal Ligated Chickens

  • 손장호 (대구교육대학교 실과교육과)
  • Son, Jang-Ho (Department of Practical Arts Education, Daegu National University of Education)
  • 투고 : 2010.11.16
  • 심사 : 2011.01.11
  • 발행 : 2011.03.31

초록

본 연구는 맹장 결찰계를 이용하여서 [15N]urea의 이용성을 조사하였다. 질소의 balance 및 이용률은 맹장 결찰 수술에 의해서 높아지는 경향이 인정되었다. 이는 맹장 결찰 수술로 인한 요산 배설량의 유의한 감소로 인한 결과였다(사료 및 요소 유래 질소 이용률, P<0.01). 닭에 있어서 맹장 결찰 수술은 사료 유래 ammonia 배설량은 증가(P<0.01), 요소 유래 ammonia배설량은 감소(P<0.01)시켰다. 요소 유래, 요소질소의 배설량은 맹장 결찰 수술에 의해서 유의하게 증가하였지만(P<0.01), 맹장 결찰계도 5% 단백질 사료와 같이 급여한 요소를 51.6%정도 이용하는 것으로 나타났다. 결론적으로 닭에 있어서 단백질 수준이 결핍된 사료와 같이 급여된 요소의 이용에 있어서 맹장은 유의한 역할을 한다고 할 수 있다. 하지만 질소의 이용에 있어서는 반드시 긍정적인 역할만을 한다고는 결론내릴 수 없다.

The effect of cecal ligation on the utilization of dietary [15N]urea in chickens fed 5 % protein diet plus urea were investigated. Nitrogen balance and utilization tended (P<0.01) to increase by cecal ligation. Total uric acid excretion was significantly decrease by (P<0.01) cecal ligation in chickens from origin of diet and urea (P<0.01). Excretion of ammonia was increased in chickens from origin of diet, where as it decreased in chickens an urea diet (P<0.01). Amount of urea nitrogen excretion from origin of urea was significantly decrease (P<0.01) by cecal ligation, but cecal ligated chicken fed 5% protein diet with urea showed 51.6% urea utilization. Result obtained in present study indicates that ceca is having beneficial role for urea utilization in chicken fed protein deficient diets, but ceca do not always positive role for nitrogen utilization.

키워드

참고문헌

  1. Akester AR, Anderson RS, Hill KJ, Osbaldiston GW 1967 A radiographic study of urine flow in the domestic fowl. Bri Poult Sci 8:209-212. https://doi.org/10.1080/00071666708415670
  2. Barnes EM, Impey CS 1974 The occurrence and properties of uric acid decomposing anaerobic bacteria in the avian caecum. J Applied Bacte 37:393-409. https://doi.org/10.1111/j.1365-2672.1974.tb00455.x
  3. Isshiki Y 1976 The effect of amputating the blood vessels and nerves distributed along the cecum on the excretion of cecal feces in chickens. Jap Poult Sci 16:344-348.
  4. Isshiki Y 1979 The effect of intravenous injection of glutamic acid or uric acid on the cecal secretion of nitrogenous compounds in chickens. Animal Sci and Tech 50:855-858.
  5. Karasawa Y 1989 Effect of colostomy on nitrogen nutrition in the chicken fed a low protein diet plus urea. J Nutr 119:1388-1391. https://doi.org/10.1093/jn/119.10.1388
  6. Karasawa Y, Maeda M 1992 Effect of colostomy on the utilization of dietary nitrogen in the fowl fed on a low protein diet. Bri Poult Sci 33:815-820. https://doi.org/10.1080/00071669208417523
  7. Karasawa Y, Umemoto M, Koh K 1993 Effect of dietary protein and urea on in vitro caecal ammonia production from urea and uric acid in cockerels. Bri Poult Sci 34:711-714. https://doi.org/10.1080/00071669308417629
  8. Karasawa Y, Maeda M 1994 Role of caece in the nitrogen nutrition of the chicken fed on a moderate protein diet or a low protein diet plus urea. Bri Poult Sci 35:815-820.
  9. Karasawa Y, Ono T, Koh K 1994 Inhibitory effect of penicillin on caecal urease activity in chickens fed on a low protein diet plus urea. Bri Poult Sci 35:157-160. https://doi.org/10.1080/00071669408417681
  10. Karasawa Y, Son JH, Koh K 1997 Ligation of caeca improves nitrogen utilisation and decreases urinary uric acid excretion in chickens fed on a low protein diet plus urea. Bri Poult Sci 38:439-441. https://doi.org/10.1080/00071669708418017
  11. Lai HC, Duke GE 1978 Colonic motility in domestic turkeys. American J Dig Dis 23:673-681. https://doi.org/10.1007/BF01072351
  12. Leveille GA, Fisher H 1958 The amino acid requirements for maintenance in the adult rooster. I. Nitrogen and energy requrements in normal and protein depleted animals receivung whole egg protein and amino acid diets. J Nutr 66:441-453. https://doi.org/10.1093/jn/66.3.441
  13. March BE, Biely J 1971 Urea tolerance in growing and adult chickens. Poultry Sci 13:234-249.
  14. McLelland J 1989 Anatomy of the avian cecum. J Experimental Zool 3:2-9.
  15. McNabb FMA, McNabb RA 1975 Proportion of ammonia, urea, urate and total nitrogen in avian urine and quantitative methods for their analysis on a single urine sample. Poultry Sci 54:1498-1505. https://doi.org/10.3382/ps.0541498
  16. Mortensen A, Tindall AR 1984 The role of urea in nitrogen excretion and cecal nitrogen metabolism in willow ptarmigan. J Comparative Physiol 155B:71-74.
  17. O'Dell BL, Wood WD, Laerdal OA, Jeffay AM, Savage JE 1960 Distribution of the major nitrogenous compounds and amino acids in chicken urine. Poultry Sci 39:426-431. https://doi.org/10.3382/ps.0390426
  18. Okuda H, Fujii S 1966 Determination of blood ammonia by the spectrophotometric method. Saishin Igaku 21:622-627.
  19. Okumura J, Tasaki I. 1968 Urinary nitrogen excretion in fowls fed acid or alkali. J Nutr 95(2):148-152. https://doi.org/10.1093/jn/95.2.148
  20. Popema TF, Duke GE 1992 The effectiveness of ligation or detaching ceca as an alternative to cecectomy. Poultry Sci 71:1384-1390. https://doi.org/10.3382/ps.0711384
  21. Pudelkiewicz WJ, Stutz WW, Matterson LD 1968 Determination of uric acid in avian excreta by use of uricase and differential spectrophotometry. Poultry Sci 47:1274-1277. https://doi.org/10.3382/ps.0471274
  22. Son JH, Karasawa Y, Koh K 1996a Comparative effect of ligation of ceca on nitrogen utilization and nitrogen excretion in chickens fed a low protein diet or a low protein diet plus urea. Animal Sci and Tech 67(2):171-174.
  23. Son JH, Karasawa Y, Koh K 1996b Effects of ligation of ceca on nitrogen utilization and nitrogen excretion in chicken fed a moderate protein diet or a moderate protein diet plus urea. Jap Poult Sci 33(3):193-197. https://doi.org/10.2141/jpsa.33.193
  24. Son JH, Karasawa Y, Nahm KH 1997 Effect of cecectomy on nitrogen utilization and nitrogen excretion in chickens fed a low protein diet supplied with urea. Asian-Aust J Anim Sci 10(3):274-276. https://doi.org/10.5713/ajas.1997.274
  25. Son JH, Karasawa Y 2000 Effect of removal of caecal contents on nitrogenutilization and nitrogen excretion in caecally ligated chickens fed a low protein supplemented with urea. Bri Poult Sci 41:69-71.
  26. Son JH, Karasawa Y, Nahm KH 2000 Effect of caecectomy on growth, moisture in excreta, gastrointestinal passage time and uric acid excretion in growing chicks. Bri Poult Sci 41:72-74. https://doi.org/10.1080/00071660086439
  27. Son JH, Ragland D, Adeola O 2002 Quantification of digesta flow into the caeca of roosters. Bri Poultry Sci 43:322-324. https://doi.org/10.1080/00071660120121562
  28. Son JH, Karasawa Y 2003 Comparative effect of caecal ligation and colostomy on nitrogen utilisation in chickens fed low protein or urea-supplemented low protein diets. J Poult Sci 40:92-100. https://doi.org/10.2141/jpsa.40.92
  29. Son JH, Karasawa Y 2004 A study on the back flow of urine into caeca of roosters. J Poult Sci 41:186-192. https://doi.org/10.2141/jpsa.41.186
  30. Steel RGD, Torrie JH 1980 Principles and procedures of statistics. Int Student Ed. McGraw Hill, Tokyo