• Title/Summary/Keyword: nitrogen ($N_2$)

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Nitrogen Balance and Biological Nitrogen Fixation of Soybean in Soybean-Barley Cropping System

  • Park Sei Joon;Kim Wook Han;Lee Jae Eun;Kwon Young Up;Shin Jin Chul;Ryu Yong Hwan;Seong Rak Chun
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.50 no.1
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    • pp.1-4
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    • 2005
  • This experiment was conducted to investigate the soil nitrogen credit of biological nitrogen fixation (BNF) and the nitrogen balance of soybean in soybean-barley cropping systems. Soybean cultivar, Shinpaldalkong2 and barley cultivar, Olbori, were used in soybean mono-cropping (SM), barley monocropping (BM), and barley­soybean double cropping system. The barley-soybean double cropping system was treated with two different levels of nitrogen fertilizers, 0 nitrogen fertilizer (BS-F0), and standard nitrogen fertilizer (BS-F1). Nitrogen and organic matter concentrations in soil of BS-F1 plot on October, 2001 were increased $4.8\%\;and\;5.9\%$, respectively, compared with those on October, 2000. The ranges of BNF rate in soybean were $69.1\~ 88.2\%$ in two years, and the rate was the highest in BS-F0 plot and the lowest in SM plot. The ranges of nitrogen harvest index (NHI) in all treatments were $83.9\~86.7\%$. The yield was 270 kg/10a in BS­F1 plot and 215 kg/10a in BS-F0 plot. However, the nitrogen balances were +0.6 kg/10a of gain of soil nitrogen in BS-F0 plot and -0.4 kg/10a of loss of soil nitrogen in BS-F1 plot. In comparisons of SM and BS-F1 plots, although the seed yields were similar in two plots, the loss of soil nitrogen was higher in SM than BS-F1 plot. Overall, our results suggest that barley-soybean double cropping system was more effective in respect to seed productivity and soil nitrogen conservation than soybean monocropping system, and the N credit to following crops by soybean cultivation was identified in soybean double cropping system.

Growth of an Obligatory Methanotroph Methylosinus trichosporium OB3b on Methanol (메탄자화균 Methylosinus trichosporium OB3b의 메탄올 기질에서의 성장)

  • 강문선;황재웅박성훈
    • KSBB Journal
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    • v.10 no.2
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    • pp.212-220
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    • 1995
  • An obligatory type II methanotroph Methylosinus trichosporium OB3b was cultivated on methanol as a sole carbon and energy source. The effects of methanol concentration, pH, temperature, nitrogen source and phosphate concentration on cell growth were investigated and the results were compared with the growth on methane, which had been studied previously. When $(NH_4)_2SO_4$ was used as a nitrogen source, the maximal specific growth rate (${\mu}max$) on methanol was $0.20hr^{-1}$ and the carbon conversion efficiency(CCE) was 43%. In comparison, on methane, ${\mu}max$ and CCE were $0.08hr^{-1}$ and 32%, respectively. Ammonia was found to be a better nitrogen source for methanol-growing cells. Cell yield on nitrogen (YX/N) was the same regardless of nitrogen source as 7.14g dry cells/g N, but the yield on methanol(YX/N) was higher with ammonia(0.8g dry cells/g MeOH) than with nitrate(0.64g dry cells/g MeOH). Optimal pH and temperature were 7.0 and $30^{\circ}C$, respectively. Methanol inhibition on cell growth was observed at above 0.5%(v/v). Inhibition by phosphate was observed at above 60mM, although the inhibition on methanol dehydrogenase activity started at a much lower level of 20mM. Based on the experimental findings, the cellular physiology of M. trichosporium OB3b growing on the two closely-related carbon sources were discussed extensively.

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The Effect of Minaral Nitrogen Fertilization on Grassland Production under Various Cutting Frequencies I. Dry matter yield and estimation of optimum rate of mineral nitrogen fertilization in orchardgrass(Dactylis glomerata L) (예취빈도에 따른 무기태 질소시비가 초지의 생산성에 미치는 영향 I. 오차드 그라스의 건물수량과 적정 질소시비 수준의 추정)

  • 조익환;이주삼;안종호
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.14 no.2
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    • pp.69-75
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    • 1994
  • This experiment was canied out to study the effect of cutting frequencies (3, 4 and 5 cuts per year) and mineral nitrogen fertilization on dry matter yields, in order to estimate optimum level of fertilizing mineral nitrogen in orchardgrass(Dacry1is glomerafa L.). The results are as follows: 1. Dry matter yields were the highest in the 1st cut at 3 cutting frequency and in the 2nd cut at 4 and 5 cutting frequencies, and they shared 49.7, 37.0 and 37.0% of annual dry matter yield respectively. 2. When only phosphorus and potassium were applied, annual dry matter yields were between 9.4 and 11.5 tons per ha and the highest yield was observed at 3 cutting frequency. 3. Dry matter yield in relation to fertilization of mineral nitrogen was highly increased when more nitrogen was applied as 360 kg per ha compared to 240 kg per ha at 3 cutting frequency. At 4 and 5 cutting frequencies, increases in dry matter yield, to large extent, were showed at 120 and 150 kg of nitrogen per ha(30 kg Nlcutlha) compared to no application of nitrogen. The efficiencies of mineral nitrogen fertilization (kg DM/kg N) were 28.0, 22.7 and 20.6 kg dry matter yields per nitrogen(kg) respectively). 4. At 4 and 5 cutting frequencies, dry matter yields were reduced when 360 kg and 300 kg of mineral nitrogen per ha(90 kg and 60 kg Nlcutlha) were applied respectively. 5. Particularly at 4 and 5 cutting frequencies of this study, maximum marginal yields reached at 129.9 kg and 148.0 kg of fertilizing mineral nitrogen per ha, and economical borden of mineral nitrogen fertilization were between 222.4 and 250.0 kg and between 244.8 and 276.8 kg respectively. At the same cutting frequencies, the highest dry matter yields were obtained at 365.4 and 433.8 kg of fertilizing mineral nitrogen respectively.

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Genetic Engineering of Biological Nitrogen Fixation and its Application to Agronomy - Selection of Rhizobium japonicum Mutants having Greater Symbiotic Nitrogen Fixing Activity with Soybean - (질소고정(窒素固定)의 유전공학(遺傳工學的) 연구(硏究) 및 농업(農業)에의 응용방안(應用方案) - 대두(大豆)에 효율적인 공생질소고정(共生窒素固定)을 할 수 있는 Rhizobium japonicum mutant의 선별 -)

  • Cho, Moo Je;Yang, Min Suk;Yun, Han Dae;Choe, Zhin Ryong;Choe, Yong Lark;Kang, Kyu Young
    • Microbiology and Biotechnology Letters
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    • v.13 no.1
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    • pp.79-85
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    • 1985
  • Rhizobium japonicum wild type strains isolated from local soybean variety Jangback root nodules with higher nitrogenase activity than R. japonicum 3I1110 or 61A76 was mutangenized by N-methyl-N'-nitro-N-nitrosoguanidine and UV-irradiation, and screened by effectiveness assay with soybean. One mutant strain JB65 nodulated the roots earlier than the wild type and also expressed higher acetylene-reducing activity in the presence and absence of fixed nitrogen. The selected mutant was compared with SM35 strain and showed greater nodulation and symbiotic nitrogen fixing activity with local soybean variety Jangback than SM35 strain.

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Properties of the gate dielectrics by thermal oxidation in ${N_2}O$ gas (${N_2}O$ 가스로 열산화된 게이트 유전체의 특성)

  • 김창일;장의구
    • Electrical & Electronic Materials
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    • v.6 no.1
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    • pp.55-62
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    • 1993
  • 수소 관련된 species를 포함하지 않고 자기제한특성으로 초박막 성장을 용이하게 제어할 수 있는 N$_{2}$O 가스 분위기에서 실리콘의 산화는 질화된 산화막의 재산화공정 보다 훨씬 간단한 공정이다. N$_{2}$O산화로 형성된 Si-SiO$_{2}$ 계면에서 nitrogen-rich층은 산화막 구조를 강화할 뿐만 아니라 게이트 유전체의 질을 개선하고 산화율을 감소시키는 산화제의 확산 장벽으로 작용한다. 초박막 oxynitride 게이트 유전체가 종래의 열산화 방법으로 제작되었고 oxynitride막의 특성이 AES와 I-V 특성 측정의 결과를 분석하여 연구하였다.

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Deposition Characteristic of InNx Films by Reactive DC Magnetron Sputtering (반응성 직류 스퍼터법에 의한 질화 인듐 박막의 제막 특성)

  • 송풍근;류봉기;김광호
    • Journal of the Korean Ceramic Society
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    • v.40 no.8
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    • pp.739-745
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    • 2003
  • In $N_{x}$ films were deposited on soda-lime glass without substrate heating by reactive dc magnetron sputtering using indium (In) metal target. Depositions were carried out under various total gas pressures ( $P_{tot}$) of mixture gases (Ar+$N_2$ or He+$N_2$). He gas was introduced to $N_2$ gas in order to enhance the reactivity of nitrogen on film surface by the "penning ionization". Plasma impedance decreased greatly when 20% or more introduced the $N_2$ gas. This is due to the In $N_{x}$ layers formed on target surface because a secondary electron emission rate of InN is small compared with In metal. XRD patterns of the films revealed that <001> preferred oriented polycrystalline In $N_{x}$ films, where the crystallinity of the films was improved with decrease of $P_{tot}$ and with increase of $N_2$ flow ratio. The improvement of the crystallinity and stoichimetry of the In $N_{x}$ films were considered to be caused by an increase in the activated nitrogen radicals and also by an increase in the kinetic energy of sputtered In atoms arriving at growing film surface, which should enhance the chemical reaction and surface migration on the growing film surface, respectively. Furthermore, the films deposited using mixture gases of He+$N_2$ showed higher crystallinity compared with the film deposited by the mixture gases of Ar+$N_2$.$.EX>.

Variation of Nitrogen Removal Efficiency and Microbial Communities Depending on Operating Conditions of a CANON Process (CANON 공정에서 운전조건에 따른 질소 제거효율 및 미생물군집 변화)

  • Jo, Kyungmin;Park, Younghyun;Cho, Sunja;Lee, Taeho
    • Journal of Korean Society of Environmental Engineers
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    • v.37 no.6
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    • pp.332-339
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    • 2015
  • Nitrogen removal is one of the most important issues about wastewater treatment because nitrogen is a primary pollutant caused various problems such as eutrophication. We developed a CANON microbial community by using AOB and ANAMMOX bacteria as seeding sources. When 100 mg-N/L of influent ammonium was supplied, the DO above 0.4 mg/L showed a very low TN removal efficiency while the DO of 0.3 mg/L showed TN removal efficiency as high as 71.3%. When the influent ammonium concentration was reduced to 50 mg/L, TN removal efficiency drastically deceased. However, TN removal efficiency was recovered to above 70% after 14 day operation when the influent nitrogen concentration was changed again from 50 mg-N/L to 100 mg-N/L. According to the operating temperature from $37{\pm}1^{\circ}C$ to $20{\pm}1^{\circ}C$, TN removal efficiency also rapidly decreased but gradually increased again up to $70.0{\pm}2.6$%. The analysis of PCR-DGGE showed no substantial difference in microbial community structures under different operational conditions. This suggests that if CANON sludge is once successfully developed from a mixture of AOB and ANAMMOX bacteria, the microbial community can be stably maintained regardless of the changes in operational conditions.

The Estimation of Optimal Nitrogen and Lime Application Levels on the Growth Control of Sheep Sorrel(Rumex acetocella L.) in Mountainous Pastures (산지초지에서 애기수영의 생육제어를 위한 질소와 석회의 적정시용수준의 추정)

  • Lee, Ju Sam;Lee, Sang Young;Jo, Ik Hwan
    • Journal of The Korean Society of Grassland and Forage Science
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    • v.19 no.4
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    • pp.363-372
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    • 1999
  • This experiment was carried out to estimate an optimal lime and nitrogen levels on the growth control of sheep sorrel in mountainous pastures of Taekwalyon areas. The dry matter yield of sheep sorrel was recongnized significantly differences between the N levels and lime levels, respectively. Most effective growth control of sheep sorrel showed an over the N levels of 21kg/10a under the ranges of lime levels of 200~300kg/10a per annum. The highest dry matter yield of sheep sorrel was obtained at the N level of 16kg/10a under the N level of 48kg/10a per annum. The highest growth control effect of sheep sorrel was obtained at 2nd and 4th cuts, and 1st, 2nd and 4th cuts under the ranges of lime levels of 200~300kg/10a, and the N level of 48kg/10a per annum, respectively.

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Development of Economical Fertilizer-Based Media for Mass Culturing of Nannochloropsis oceanica

  • Bae, Jean-Hee;Hur, Sung-Bum
    • Fisheries and Aquatic Sciences
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    • v.14 no.4
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    • pp.317-322
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    • 2011
  • This study was conducted to develop economical agricultural fertilizer media for the mass culturing of Nannochloropsis oceanica. Specific growth rates of N. oceanica cultured with differing concentrations of commercial compounds, urea fertilizers, and trace elements (Zn, Cu, Co, Mo) were compared with the growth rate in f/2 medium. Among the various added trace elements, $CuSO_4{\cdot}5H_2O$ was most effective for high growth of N. oceanica. The main nitrogen source in the agricultural fertilizers was ammonium, which was unsuitable for the growth of N. oceanica. Thus, the fertilizer at a lower concentration infused with $NaNO_3$ as a nitrogen source was more effective than fertilizer at higher concentrations. In this study, the growth of N. oceanica cultured with an agricultural fertilizer medium composed of compound fertilizer (41.7 mg/L), urea fertilizer (34.4 mg/L), $NaNO_3$ (150 mg/L), and $CuSO_4{\cdot}5H_2O$ (0.0588 mg/L) was similar to that of N. oceanica cultured in f/2 medium.

Synthesis of Aluminum Nitride Powers and Whiskers from a (NH4)[Al(edta)]·2H2O Complex under a Flow of Nitrogen (질소 분위기에서 (NH4)[Al(edta)]·2H2O 착물으로부터 질화알루미늄 분말 및 휘스커의 합성)

  • Jung, Woo-Sik
    • Journal of the Korean Ceramic Society
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    • v.39 no.3
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    • pp.272-277
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    • 2002
  • Aluminum nitride (AlN) powders and whiskers were synthesized by a modified carbothermal reduction and nitridation where a ($NH_4)[Al(ethylenediaminetetraacetate)]{\cdot}2H_2O$ complex is used as precursor. The AlN powders were obtained by calcining the complex without mixing any carbon source under a flow of nitrogen in the temperature range 1200∼1500$^{\circ}$C and then burning out the residual carbon. The nitridation process was investigated by $^{27}Al$ magic-angle spinning (MAS) unclear magnetic resonance, infrared spectroscopy and X-ray diffraction. The complex is pyrolyzed, converted to ${\rho}$- and ${\gamma}$- alumina and then nitridated to AlN without ${\gamma}-{\alpha}$ alumina transition. The morphology of ${\gamma}$-alumina, when it was converted to AlN, was retained, strongly indicating that ${\gamma}$-alumina is converted to AlN through solid-state $AlO_xN_y$, not through gaseous intermediates such as aluminum and aluminaum suboxides. AlN whiskers were obtained, when a (0001) sapphire was used as a catalyst.