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http://dx.doi.org/10.5657/FAS.2011.0317

Development of Economical Fertilizer-Based Media for Mass Culturing of Nannochloropsis oceanica  

Bae, Jean-Hee (Department of Marine Bio-materials and Aquaculture, Pukyong National University)
Hur, Sung-Bum (Department of Marine Bio-materials and Aquaculture, Pukyong National University)
Publication Information
Fisheries and Aquatic Sciences / v.14, no.4, 2011 , pp. 317-322 More about this Journal
Abstract
This study was conducted to develop economical agricultural fertilizer media for the mass culturing of Nannochloropsis oceanica. Specific growth rates of N. oceanica cultured with differing concentrations of commercial compounds, urea fertilizers, and trace elements (Zn, Cu, Co, Mo) were compared with the growth rate in f/2 medium. Among the various added trace elements, $CuSO_4{\cdot}5H_2O$ was most effective for high growth of N. oceanica. The main nitrogen source in the agricultural fertilizers was ammonium, which was unsuitable for the growth of N. oceanica. Thus, the fertilizer at a lower concentration infused with $NaNO_3$ as a nitrogen source was more effective than fertilizer at higher concentrations. In this study, the growth of N. oceanica cultured with an agricultural fertilizer medium composed of compound fertilizer (41.7 mg/L), urea fertilizer (34.4 mg/L), $NaNO_3$ (150 mg/L), and $CuSO_4{\cdot}5H_2O$ (0.0588 mg/L) was similar to that of N. oceanica cultured in f/2 medium.
Keywords
Agricultural fertilizer; Economical media; f/2 media; Nannochloropsis oceanica;
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1 Schreiber E. 1927. Die Reinkultir von marinem phytoplankton und deren bedeutung für die erforschung der produktionsfahigkeit des meerwassers. Wiss Meersuntersuch NF 16, 1-34.
2 Seychelles LH, Audet C, Tremblay R, Fournier R and Pernet F. 2009. Essential fatty acid enrichment of cultured rotifers (Brachionus plicatilis, Muller) using frozen-concentrated microalgae. Aqauc Nutr 15, 431-439.   DOI   ScienceOn
3 Stein JR. 1973. Handbook of Phycological Methods: Culture Methods and Growth Measurement. Cambridge University Press, Cambridge, GB.
4 Sukenik A, Zmora O and Carmeli Y. 1993. Biochemical quality of marine unicellular algae with special emphasis on lipid composition. II. Nannochloropsis sp. Aquaculture 117, 313-326.   DOI   ScienceOn
5 Ukeles R. 1980. American experience in the mass culture of microalgae for feeding larvae of the American oyster, Crassostrea virginica. In: Algae Biomass: Production and Use. Shelef G and Soeder CJ, eds. Elsevier/North Holland Biomedical Press, Amsterdam, NL, pp. 287-306.
6 Valenzuela-Espinoza E, Millan-Nunez R and Nunez-Cebrero F. 2002. Protein, carbohydrate, lipid and chlorophyll a content in Isochrysis aff. galbana (clone T-Iso) cultured with a low cost alternative to the f/2 medium. Aquac Eng 25, 207-216.   DOI   ScienceOn
7 Volkman JK, Brown MR, Dunstan GA and Jeffrey SW. 1993. The biochemical composition of marine microalgal from the class Eustigmatophyceae. J Phycol 29, 69-78.   DOI
8 Walne PR. 1966. Experiments in the large-scale culture of the larvae of Ostrea edulis L. Fish Invest London Ser II 25, 1-53.
9 Zittelli GC, Lavista F, Bastianini A, Rodolfi L, Vincenzini M and Tredici MR. 1999. Production of eicosapentaenoic acid by Nannochloropsis sp. cultures in outdoor tubular photobioreactors. J Biotechnol 70, 299-312.   DOI   ScienceOn
10 Guillard RRL and Ryther JH. 1962. Studies of marine plankton diatoms. I. Cyclotella nana Hustedt, and Detonula confervacea (Cleve) Gran. Can J Microbiol 8, 229-239.   DOI   ScienceOn
11 Harting P, Grobbelaar JU, Soeder CJ and Groeneweg J. 1988. On the mass culture of microalgae: a real density as an important factor for achieving maximal productivity. Biomass 15, 211-221.   DOI   ScienceOn
12 Hu H and Gao K. 2003. Optimization of growth and fatty acid composition of a unicellular marine picoplankton, Nannochloropsis sp., with enriched carbon sources. Biotechnol Lett 25, 421-425.   DOI   ScienceOn
13 Kalpan D, Richmond AE, Dubinsky Z and Aaronson S. 1986. Algal nutrition. In: Handbook of Microalgal Mass Culture. Richmond A, ed. CRC Press, Boca Raton, FL, US, pp. 147-197.
14 Kobayashi T, Nagase T, Kurano N and Hino A. 2005. Fatty acid composition of the L-type rotifer Brachionus plicatilis produced by a continuous culture system under the provision of high density Nannochloropsis. Nippon Suisan Gakkaishi 71, 328-334.   DOI   ScienceOn
15 Lopez-Ruiz JL, Garcia-Garcia RG, Soledad M and Almeda F. 1995. Marine microalgae culture: Chaetoceros gracilis with zeolitic product Zestec-56 and a commercial fertilizer as a nutrient. Aqauc Eng 14, 367-372.   DOI   ScienceOn
16 Lubian LM, Montero O, Moreno-Garrido I, Huertas IE, Sobrino C, Gonzalez-del Valle M and Pares G. 2000. Nannochloropsis (Eustigmatophyceae) as source of commercially valuable pigments. J Appl Phycol 12, 249-255.   DOI   ScienceOn
17 Okauchi M, Yamada T and Ozaki A. 2008. Optimum media for outdoor large-scale and indoor small-scale batch style culture of Nannochloropsis oculata. Aquac Sci 56, 147-155.
18 Brown MR, Jeffrey SW, Volkman JK and Dunstan GA. 1997. Nutritional properties of microalgae for mariculture. Aquaculture 151, 315-331.   DOI   ScienceOn
19 Pacheco-Vega JM and Sanchez-Saavedra MDP. 2009. The biochemical composition of Chaetoceros muelleri (Lemmermann Grown) with an agricultural fertilizer. J World Aquac Soc 40, 556-560.   DOI   ScienceOn
20 Patil V, Kallqvist T, Olsen E, Vogt G and Gislerød HR. 2007. Fatty acid composition of 12 microalgae for possible use in aquaculture feed. Aquacult Int 15, 1-9.   DOI   ScienceOn
21 Cabrera T and Hur SB. 2001. The nutritional value of live foods on the larval growth and survival of Japanese flounder, Paralichthys olivaceus. J Appl Aquc 11, 35-53.
22 Cabrera T, Bae JH, Bai SC and Hur SB. 2005. Effects of microalgae and salinity on the growth three types of the rotifer Brachionus plicatilis. J Fish Sci Technol 8, 70-75.
23 Cha SH, Kim MJ, Yang HY, Jin CB, Jeon YJ, Oda T and Kim D. 2010. ACE, $\alpha$-glucosidase and cancer cell growth inhibitory activities of extracts and fractions from marine microalgae, Nannochloropsis oculata. Korean J Fish Aquat Sci 43, 437-444.   DOI   ScienceOn
24 Chisti Y. 2007. Biodiesel from microalgae. Biotechnol Adv 25, 294-306.   DOI   ScienceOn
25 Duncan DB. 1955. Multiple range and multiple F tests. Biometrics 11, 1-42.   DOI   ScienceOn
26 Fabregas J, Herrero C, Cabezas B and Abalde J. 1985. Mass culture and biochemical variability of the marine microalgae Tetraselmis suecica Kylin (Butch) with high nutrient concentrations. Aquaculture 49, 231-244.   DOI   ScienceOn
27 Guillard RRL 1973. Division rates. In: Handbook of Phycological Methods. Culture Method and Growth Measurement. Stein JR, ed. Cambridge University Press, Cambridge, GB, pp. 289-311.
28 Fabregas J, Toribio L, Abalde J, Cabezas B and Herrero C. 1987. Approach to biomass production of the marine microalga Tetraselmis suecica (Kylin) butch using common garden fertilizer and soil extract as cheap nutrient supply in batch cultures. Aquac Eng 6, 141-150.   DOI   ScienceOn
29 Ferreira M, Coutinho P, Seixas P, Fabregas J and Otero A. 2009. Enriching rotifers with "premium" microalgae. Nannochloropsis gatosynthesis ditana. Mar Biotechnol 11, 585-595.   DOI   ScienceOn
30 Gonzalez-Rodriguez E and Maestrini SY. 1984. The use of some agricultural fertilizers for the mass production of marine algae. Aquaculture 36, 245-256.   DOI   ScienceOn
31 Becker EW. 1981. Algae mass cultivation: production and utilization. Process Biochem 16, 10-14.
32 Admiraal W. 1977. Tolerance of estuarine benthic diatoms to high concentrations of ammonia, nitrite ion, nitrate ion and orthophosphate. Mar Biol 43, 307-315.   DOI   ScienceOn
33 Andrade LR, Farina M and Amado GM. 2004. Effects of copper on Enteromorpha flexuosa (Chlorophyta) in vitro. Ecotoxicol Environ Saf 58, 117-125.   DOI   ScienceOn
34 Bae JH. 2004. Selection of seasonal optimum Chlorella and Nannochloris species and development of media for mass culture. Ph.D. Dissertation, Pukyong National University, Busan, KR.
35 Borowitzka MA. 1997. Microalgae for aquaculture: opportunities and constraints. J Appl Phycol 9, 393-401.   DOI   ScienceOn
36 Borowitzka MA and Borowitzka LJ. 1988. Micro-algal Biotechnology. Cambridge University Press, New York, US.