• Preventive Nutrition and Food Science
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• 제15권2호
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• pp.92-97
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• 2010

Luteolin and chicoric acid are the most abundant phytochemicals in dandelion (Taraxacum officinale) leaf. In this study, four kinds of extraction methods [hot water, ambient temperature (AT) water, ethanol, and methanol] were applied to analyze the contents of both phytochemicals and verify their anti-inflammatory and antioxidative activities. The methanol extract showed the most potent nitric oxide (NO) inhibitory effect. The luteolin and chicoric acid concentrations were 3.42 and $12.86\;{\mu}g/g$ dandelion leaf in the methanol extract. The NO-suppressive effect of luteolin and chicoric acid was identified in a dose-dependent manner with $IC_{50}$ values of $21.2\;{\mu}M$ and $283.6\;{\mu}M$, respectively, in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells without cytotoxicity. Malondialdehyde (MDA) concentration, as an index for free radical injury on cell membrane, was also dose-dependently inhibited by the two compounds. The suppressive effect was further examined using mRNA and protein expression levels, which were attributable to the inhibition of inducible nitric oxide synthase (iNOS). These results suggest that two phytochemicals in dandelion leaf, luteolin and chicoric acid, may play an important role in the amelioration of LPS-induced oxidative stress and inflammation.

• 한국약용작물학회지
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• 제26권2호
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• pp.119-126
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• 2018

Background: The flowering plant Hippophae rhamnoides L. has been used for many studies on fruit or leaf extracts. This study was conducted to investigate the development of a new cosmetic material from H. rhamnoides fruits and leaves that have by antioxidant, anti-inflammatory and wrinkle improvement activities. Methods and Results: The antioxidant abilities of H. rhamnoides extracts, including of a water-soluble fruit powder (FW), a fatsoluble fruit powder (FF), a supercritical extract of fruit by-product (BS), and a mixture of leaf and fruit (MIX), were investigated in vitro. A DPPH radical assay for antioxidant activity was performed for these fractions alongside assay to evaluate the total phenolic and flavonoid content (TPC and TFC). As expected, the MIX had the highest DPPH radical scavenging activity ($RC_{50}=10.27{\mu}g/m{\ell}$), and the TPC and TFC also were highest in MIX ($225.7mg{\cdot}GAE/g$, and $25.18mg{\cdot}QE/g$, respectively). Nitric oxide (NO) production in LPS-induced RAW264.7 cells was estimated and the results indicated an over 75% decrease of NO production in FF and MIX. In other assays, the highest elastase inhibitory activity was found in FW. Conclusions: These results revealed that H. rhamnoides extracts have a high potential for antioxidant, anti-inflammatory and antiwrinkle activities. H. rhamnoides products are suggested to be applied as the functional materials of cosmetic ingredients.

• 생명과학회지
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• 제29권4호
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• pp.428-435
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• 2019

알래스카 산 대구(Gadus chalcogrammus)는 대구과(family Gadidae)의 해산어종이다. 알래스카 명란 원료, 고추장 명란, 발효 명란을 증류수와 에탄올로 추출하여 2, 2- diphenyl - 1- picrylhydroazyl (DPPH), 하이드록시 라디칼(OH), 산화질소(NO)의 항산화 소거능을 평가하였다. 철 이온 환원력(FRAP)을 조사하였다. ABTS+ 소거능은 Brand-Williams 등의 방법에 따랐다. 고추장 명란의 증류수 추출물의 DPPH 소거능은 1.0 mg/ml 농도에서 71.9%였으며 에탄올 추출물에서 소거능은 73.7%였다. 발효 명란의 증류수 추출물의 DPPH 소거능은 1.0 mg/ml 농도에서 78.0%였고 에탄올 추출물에서 소거능은 78.4%였다. 명란 원료의 증류수 추출물과 에탄올 추출물의 DPPH에 대한 최소 저해 농도 값($IC_{50}$)은 각각 11.65 ug/ml과 11.47 ug/ml이었다. 명란 원료, 고추장 명란, 발효 명란의 에탄올 추출물의 OH 소거능은 1.0 mg/ml 농도에서 70.9%, 79.0, and 80.6%였다. 명란 원료의 증류수 추출물과 에탄올 추출물의 NO의 최소 저해 농도 값은 11.45 ug/ml과 11.41 ug/ml였다. 본 연구 결과, 고추장 명란과 발효명란은 원료 명란 보다 높은 DPPH, OH, NO 소거능을 나타내었다. 고추장 명란과 발효 명란은 자연 라디칼 소거능이 있어 식품으로써 항산화 보충제가 될 수 있을 것이다.

• 대한약침학회지
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• 제10권2호통권23호
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• pp.25-30
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• 2007

Objectives : Free radical metabolism seems to occupy a remarkably common position in the mechanisms of aging and aging related disease. Oxidative damage to DNA, lipids. proteins and other molecules may contribute to the development of cancer, cardiovascular disease and possibly neurodegenerative disease. This study was designed to find out whether Carthami Flos Herbal-Acupuncture Solution can scavenge Nitric Oxide(NO) or not. SNAP is NO generator. NO concentration was estimated after 2,6, 12 and 24 hrs in no treatment group, after treatment with Vit. C or 1, 10, 100${\mu}$g/m1 of Carthami Flos Herbal-Acupuncture Solution. There was no significant scavenging effect of Carthami Flos Herbal-Acupuncture Solutionon NO after 2 hrs. But there was a significant scavenging effect of Cafhami Flos Herbal-Acupuncture Solution on NO in 10${\mu}$/m1 group after 6hrs. And there was a significant scavenging effect of carthami Flos Herbal-Acupuncture Solution on NO in 1, 10${\mu}$g/ml group after 12, 24 hrs. These results suggest that Carhami Flos Herbal-Acupuncture Solution has scavenging effect on NO. This study shows that Carthami Flos Herbal-Acupuncture Solution can be used for aging related disease and further studies are required to investigate the antioxidative effects of it.

• Food Science and Biotechnology
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• 제18권5호
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• pp.1124-1131
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• 2009

Acanthopanax senticosus has been used in East Asia as a traditional medicine. The purpose of this study is the investigation of an extraction method for A. senticosus var subinermis (ASvS) leaves to improve their antioxidant and anti-inflammatory activities. Six extracts were prepared: extracted with water (W), ethanol (Eth), water or ethanol after ultra high pressure (WP, EthP), and water or ethanol after an extrusion process (WEx, EthEx). Diphenylpicrylhydrazyl (DPPH) radical scavenging activity and ferric reducing ability showed that all extracts had a significant antioxidant activity. In anti-inflammatory activities, Eth and EthEx significantly inhibited the release of lipopolysaccharide (LPS)-induced nitric oxide (NO), tumor necrosis factor (TNF)-${\alpha}$, and interleukin (IL)-$1{\beta}$ independent of cell viability in RAW 264.7 macrophage cells. Inducible NO synthase (iNOS) protein was significantly decreased by EthEx. These findings present that Eth and EthEx extracts of ASvS leaves have anti-inflammatory activities, and EthEx extract suppresses LPS-induced NO through the down-regulation of iNOS and pro-inflammatory cytokines.

• 생명과학회지
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• 제18권3호
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• pp.329-335
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• 2008

해양성 심층수(KDSW)는 해양심층수(DSW)와 유사한 mineral 조성을 가지고 있어 식품 및 의약분야에서 그 유용성에 대한 관심이 증대되고 있다. 본 연구에서는 KDSW 및 탈염한 Danasoo의 항산화력, 면역활성, 함암 및 당뇨에 대한 효과를 연구하였다. KDSW와 Danasoo의 항산화 활성은 DPPH radical scavenging 활성, SOD-like 활성 및 PCL 법에 의하여 항산화 활성을 조사하였다. KDSW와 Danasoo의 항산화 활성은 첨가된 농도에 의존적으로 증가하였으며, 특히 PCL법에 의한 KDSW와 Danasoo의 항산화능은 85.32과 14.02(nmol of ascorbic acid equivalent/ml)로 높은 항산화 활성을 나타내었다. Macrophage RAW 264.7 cell에서 LPS에 의하여 유도된 NO 활성은 Danasoo (20%)를 첨가에 의하여 약 30%정도의 NO 합성이 저해되었으며, 이 농도에서 세포 독성이 없는 것으로 MTT assay에 의하여 확인하였다. NO는 nitric oxide synthase에 의하여 합성되며, tumor성장 및 angiogenesis에서 중요한 역할을 하는 것으로 밝혀져 있으며, Danasoon (20%)는 위암세포와 폐암세포의 iNOS 발현을 현저히 감소시켰다. STZ에 의하여 유도된 당뇨 쥐의 혈당량은 Danasoo (20%) 식이에 의하여 대조구와 유사한 당뇨효과를 나타내었다. 이러한 결과들은 KDSW가 우수한 biological 활성을 가지고 있으며, 또한 천연 기능성 제품생산을 위한 소재로 사용될 수 있는 높은 가능성올 시사하고 있다.

• 동의생리병리학회지
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• 제22권6호
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• pp.1549-1556
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• 2008

The objective of this study were to evaluate the antioxidant activity and the anti-inflammatory effects of Angelica tenuissima (AT) which has been used widely as a traditional medicine. The antioxidant activities of AT was tested by DPPH radical scavenging, superoxide anion scavenging and nitric oxide scavenging. AT showed strong antioxidant activity in all experiment. In macrophages nitric oxide (NO) is released as an inflammatory mediator and has been proposed to be an important modulator of many pathophysiological conditions and high concentratin of NO is produced by inducible nitric oxide synthase (iNOS). In this study we have examined the inhibition effects of NO by 85% methanol extracts of AT in mouse (C57BL/6) peritoneal macrophage. AT (100, 1000 ${\mu}g/m{\ell}$) suppressed nitric oxide production and iNOS expression without any notable cytotoxicity and it also inhibited the expression of inflammatoryenzymes like cyclooxygenase-2 (COX-2). These data suggest that 85% methanol extracts of AT may possibly be used as antioxidant and anti-inflammatory agent.

• 한방안이비인후피부과학회지
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• 제17권3호
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• pp.18-25
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• 2004

This study was carried out to investigate the effects SMTG on the skin inflammatory reactions. Among the herbal ingredients of SMTG, ethanol extracts of Paeoniae obovata Radix and Acanthopanacis Cortex showed potent radical scavenging activity, more than 90$\%$ at a concnentration of 0.01$\%$, tested by DPPH(1,l-diphenyl-2-picryl-hyrazyl) method. And ethanol extract of Saururi Herba Seu Rhizoma inhibited the lipopolysaccharide-induced release of nitric oxide(NO), 94$\%$ at 50 ㎍／㎖, by the macrophage RAW 246.7 cells. In addition, the ethanol extract of Acanthopanacis Cortex showed antibacterial activities on Staphylococcus aureus that was reported to cause impetigo and atopic disease. Taken together, I expect that SMTG may be used as a drug for treatment on skin inflammation.

• 한방안이비인후피부과학회지
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• 제17권2호
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• pp.31-38
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• 2004

This study was carried out to investigate the effects herbal extracts on the skin inflammatory reactions. Among the herbal ingredients of herbal extracts, ethanol extract of Glycyrrhizae Radix showed potent radical scavenging activity, more than 86$\%$ at a concentration of 0.01$\%$, tested by DPPH(I,1-diphenyl-2-picryl-hyrazyl) method. And ethanol extract of Aurantii Immaturus Fructus inhibited the lipopolysaccharide-induced release of nitric oxide(NO), 99$\%$ at 50㎍／㎖, by the macrophage RAW 246.7 cells. In addition, the ethanol extracts of Sophorae Radix, Glycyrrhizae Radix showed antibacterial activities on Propionibacterium acnes that causes acne, Sophorae Radix, Glycyrrhizae Radix and Aurantii Immaturus Fructus on Corynebacterium xerosis that cause osmidrosis axillae, Glycyrrhizae Radix and Aurantii Immaturus Fructus on Staphylococcus aureus that was reported to cause impetigo and atopic disease. Taken together, I expect that herbal extracts may be used as a drug for treatment on skin inflammation.

• 한방안이비인후피부과학회지
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• 제17권2호
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• pp.39-47
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• 2004

This study was carried out to investigate the effects YSTG on the skin inflammatory reactions. Among the herbal ingredients of YSTG, ethanol extracts of Cinnamoni Ramulus, Polygoni Avicularis Herba and Scutellariae Radix showed potent radical scavenging activity, more than 90$\%$ at a concnentration of 0.01$\%$, tested by DPPH(I,1-diphenyl-2-picryl-hyrazyl) method. And ethanol extract of Saussureae Radix inhibited the lipopolysaccharide-induced release of nitric oxide(NO), 86$\%$ at 50㎍／㎖, by the macrophage RAW 246.7 cells. In addition, the ethanol extracts of Cinnamoni Ramulus and Polygoni Avicularis Herba showed antibacterial activities on Staphylococcus aureus that was reported to cause impetigo and atopic disease, Cinnamoni Ramulus, Saussureae Radix and Plantaginis Semen on Propionibacterium acnes that causes acne, and Cinnamoni Ramulus on Candida albicans that causes cadidiasis, Saussureae Radix on Corynebacterium xerosis that cause osmidrosis axillae. Taken together, I expect that YSTG may be used as an effective drug for treatment on skin inflammation.