• BMB Reports
• /
• 제53권8호
• /
• pp.437-441
• /
• 2020

In accordance with requirements of the ICH S7B safety pharmacology guidelines, numerous next-generation cardiotoxicity studies using human stem cell-derived cardiomyocytes (CMs) are being conducted globally. Although several stem cell-derived CMs are being developed for commercialization, there is insufficient research to verify if these CMs can replace animal experiments. In this study, in vitro high-efficiency CMs derived from human embryonic stem cells (hESC-CMs) were compared with Sprague-Dawley rats as in vivo experimental animals, and primary cultured in vitro rat-CMs for cardiotoxicity tests. In vivo rats were administrated with two consecutive injections of 100 mg/kg isoproterenol, 15 mg/kg doxorubicin, or 100 mg/kg nifedipine, while in vitro rat-CMs and hESC-CMs were treated with 5 μM isoproterenol, 5 μM doxorubicin, and 50 μM nifedipine. We have verified the equivalence of hESC-CMs assessments over various molecular biological markers, morphological analysis. Also, we have identified the advantages of hESC-CMs, which can distinguish between species variability, over electrophysiological analysis of ion channels against cardiac damage. Our findings demonstrate the possibility and advantage of high-efficiency hESC-CMs as next-generation cardiotoxicity assessment.

• The Korean Journal of Physiology and Pharmacology
• /
• 제16권5호
• /
• pp.327-332
• /
• 2012

Sertraline is a commonly used antidepressant of the selective serotonin reuptake inhibitors (SSRIs) class. In these experiments, we have used the whole cell patch clamp technique to examine the effects of sertraline on the major cardiac ion channels expressed in HEK293 cells and the native voltage-gated $Ca^{2+}$ channels in rat ventricular myocytes. According to the results, sertraline is a potent blocker of cardiac $K^+$ channels, such as hERG, $I_{Ks}$ and $I_{K1}$. The rank order of inhibitory potency was hERG > $I_{K1}$ > $I_{Ks}$ with $IC_{50}$ values of 0.7, 10.5, and 15.2 ${\mu}M$, respectively. In addition to $K^+$ channels, sertraline also inhibited $I_{Na}$ and $I_{Ca}$, and the $IC_{50}$ values are 6.1 and 2.6 ${\mu}M$, respectively. Modification of these ion channels by sertraline could induce changes of the cardiac action potential duration and QT interval, and might result in cardiac arrhythmia.

• The Korean Journal of Physiology and Pharmacology
• /
• 제20권1호
• /
• pp.119-127
• /
• 2016

Dihydropyridine (DHP) calcium channel blockers (CCBs) have been widely used to treat of several cardiovascular diseases. An excessive shortening of action potential duration (APD) due to the reduction of $Ca^{2+}$ channel current ($I_{Ca}$) might increase the risk of arrhythmia. In this study we investigated the electrophysiological effects of nicardipine (NIC), isradipine (ISR), and amlodipine (AML) on the cardiac APD in rabbit Purkinje fibers, voltage-gated $K^+$ channel currents ($I_{Kr}$, $I_{Ks}$) and voltage-gated $Na^+$ channel current ($I_{Na}$). The concentration-dependent inhibition of $Ca^{2+}$ channel currents ($I_{Ca}$) was examined in rat cardiomyocytes; these CCBs have similar potency on $I_{Ca}$ channel blocking with $IC_{50}$ (the half-maximum inhibiting concentration) values of 0.142, 0.229, and 0.227 nM on NIC, ISR, and AML, respectively. However, ISR shortened both $APD_{50}$ and $APD_{90}$ already at $1{\mu}M$ whereas NIC and AML shortened $APD_{50}$ but not $APD_{90}$ up to $30{\mu}M$. According to ion channel studies, NIC and AML concentration-dependently inhibited $I_{Kr}$ and $I_{Ks}$ while ISR had only partial inhibitory effects (<50% at $30{\mu}M$). Inhibition of $I_{Na}$ was similarly observed in the three CCBs. Since the $I_{Kr}$ and $I_{Ks}$ mainly contribute to cardiac repolarization, their inhibition by NIC and AML could compensate for the AP shortening effects due to the block of $I_{Ca}$.

• 한국정보통신학회논문지
• /
• 제16권10호
• /
• pp.2150-2162
• /
• 2012

차세대 모바일 인터넷의 진화에 따라 2G, 3G, 4G, B4G 이동통신 무선접속네트워크가 공존하게 되며, 기존의 통신 서비스 제공자들은 통합 서비스 제공자로 융합되고, 다수의 가상 서비스 제공자들이 발생하게 될 것으로 예상된다. 이러한 복잡한 미래의 융합 서비스를 제공하기 위해서 미래 인터넷에서는 무선 액세스 네트워크가 융합되고 다양한 서비스 제공자들에 의해 공유되는 무선 네트워크 가상화가 필수적이다. 따라서 본 논문에서는 미래 무선 액세스 네트워크 상에서 여러 서비스 제공자들의 다양한 무선 액세스 기술들이 유연하게 통합 운영될 수 있는 무선 액세스 네트워크 가상화를 위한 망 구조와 가상화 시나리오에 대해서 고찰한다. 본 논문에서 도출된 차세대 이동통신 네트워크를 위한 무선 액세스 망 구조 및 가상화 시나리오는 앞으로 무선 액세스 네트워크 가상화 알고리즘 개발을 위한 기틀 역할을 할 수 있을 것으로 기대된다.

• 한국작물학회지
• /
• 제47권1호
• /
• pp.54-61
• /
• 2002

The antioxidant activities of methanol extracts of sixteen samples were tested using 1.1-diphenyl-2-picryl hydrazyl(DPPH) reactivity and TBARS substances assay in vitro. The methanol extracts of the rice brans from three wild rice -O. minuta, O. rufipogon, and O. barthii-were found to be the most effective in DPPH radical scavenging activity. The next effective ones were the rice brans of Heugjinjubyeo and leaves of Tapgolbori. When tested on lipid peroxidation using a lipid peroxidation generation system mediated by $H_2O$$_2$/Fe$^{2+}$ in rat liver homogenates, the brans and hull of wild rice (O. minuta, O. rufipogon, and O. barthii) and rice bran of Heugjinjubyeo exhibited protective activities against lipid peroxidation in the order of effectiveness.s.

• International Journal of Computer Science & Network Security
• /
• 제23권7호
• /
• pp.16-22
• /
• 2023

Enhanced radio access technologies (RAT) are deployed in Next Generation Convergence Networks by the service providers so as to satisfy the basic requirements of end-users for e.g. QoS. Whenever the available resources are being shared simultaneously and dynamically by multiple users or distribution of allocated channels randomly, the deficiency of spectral resources and dynamic behavior of Network traffic in real time Networking, we may have problem. In order to evaluate the performance of our proposed algorithm, computer simulation has been performed on NS-2 simulator and a comparison with the existing algorithms has been made.

• 대한전자공학회논문지TC
• /
• 제46권1호
• /
• pp.48-59
• /
• 2009

차세대 이동 통신 시스템은 다양한 액세스 망에 연결되어, 이동 가입자에게 끊김 없는 다양한 대용량 멀티미디어 서비스를 제공하고자 하고 있다. 이러한 통신 시스템 중에 하나인 WiNGS 시스템은 기존의 네트워크 능력 보다 뛰어난 새로운 RAT(Radio Access Technology) 기술과 이들 간을 융합할 수 있는 IP 연결성을 가지는 망구조를 제공한다. 본 논문은 WiNGS 시스템에서 핸드 오버 시 패킷 재 정렬 문제 해결을 위해 이동 노드와 WAGW 구간 사이에 새로운 링크 계층 SR-ARQ 메커니즘을 제안한다. 또한 SR-ARQ 메커니즘 사용 시 핸드오버 시간 동안 불필요한 패킷 재전송 방지를 위해 SR-ARQ 송신노드에는 적응형 타이머를 사용한 SR-ARQ 메커니즘을, 수신 노드에서는 핸드오버 시 일시적으로 프레임의 순서가 뒤집어짐에 대해서 해당 NAK 응답을 지연하는 지연 NAK 기법을 제안한다. 그리고 제안한 기법의 성능 분석을 위해 ns-2 시뮬레이터를 이용하여, 링크 계층에 SR-ARQ를 구현하여 시뮬레이션을 수행하였으며, 시뮬레이션 결과 제안한 적응형 타이머와 지연 NAK을 사용한 SR-ARQ가 핸드오버 수행 중 불필요한 재전송을 방지하여 핸드오버 성능을 향상시킴을 보였다.

• 한국전통의학지
• /
• 제15권1호
• /
• pp.113-127
• /
• 2006

Objectives: This study was produced to examine the effects of moxibustion that had been played important role to traditional oriental medical treatment on disease. Recently, it was reported that moxi-tar which is generated in the process of moxibustion as burning combustibles decreased nitric oxide(NO) and inducible NO synthase (iNOS) generation in cellular experiments. Methods: Carrageenan-induced arthritis rat model was used to test the effect of moxi-tar as a chronic pain model. Diluted moxi-tar was single injected in several acupoints or combined with electroacupuncture (l ms, 2 Hz, and 2 mA) into contralateral ST36 acupoint for 30 min to assess the synergic effects. After the treatment, behavioral tests measuring stepping force were periodically conducted during the next 12 hours. Endogenous NO and iNOS, cyclooxygenase-2 (COX-2), and c-Fos protein expression in the spinal cord were examined on a rat model of carrageenan-induced arthritis. Results : After the induction of arthritis, rats subsequently showed a reduced stepping force of the affected limb for at least the next 4 days. The reduced stepping force of the limb was presumably due to a painful knee, since oral injection of indomethacin produced temporary improvement of weight bearing. Maxi-tar produced significant improvement of stepping force of the hindlimb affected by the arthritis lasting at least 9 hours. The magnitude of this improvement was equivalent to that obtained after an oral injection of 3 mg/kg of indomethacin and this improvement of stepping force was interpreted as an analgesic effect. Maxi-tar produced the improvement of stepping force of the affected hindlimb in a dose-dependent manner. Both NO production and iNOS, COX-2 protein expression increased by arthritis were suppressed by maxi-tar. Moxi-tar on combination with electroacupuncture (EA) produced more powerful and longer lasting improvement of stepping force of the hindlimb affected by the arthritis than either moxi-tar or EA did. Conclusion : The present study suggest that maxi-tar produces a potent analgesic effect on the chronic knee arthritis pain model in the rat and that moxi-tar-induced analgesia modulate endogenous NO through the suppression of iNOS/COX-2 protein expression.

• Journal of Communications and Networks
• /
• 제11권4호
• /
• pp.368-374
• /
• 2009

This paper focuses on a distributed multiple spectrum pricing scheme to maximize system capacity in next generation multiaccess systems, where multimode user equipments (MUEs) can connect simultaneously to multiple base stations (BSs) with multiple radio access technologies (RATs). The multi-price based scheme provides a distributed decision making for an optimal solution where radio resource allocations are determined by each MUE, unlike most centralized mechanisms where BS controls the whole radio resource. By the proposed optimal solution, MUEs can decide their share of spectrum bands and power allocation according to the spectrum price of each RAT, and at the same time the multiaccess system can achieve maximized total throughput. Numerical analysis shows that the proposed scheme achieves the maximal capacity by distributed resource allocation for the multiaccess system.

• 한국진공학회:학술대회논문집
• /
• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
• /
• pp.278.1-278.1
• /
• 2013

Among the prerequisites for stable neural interfacing are the long-term stability of electrical performance of and the excellent biocompatibility of conducting materials in implantable neural electrodes. Reduced graphene oxide offers a great potential for a variety of biomedical applications including biosensors and, particularly, neural interfaces due to its superb material properties such as high electrical conductivity, decent optical transparency, facile processibility, and etc. Nonetheless, there have been few systematic studies on the graphene-based neural interfaces in terms of biocompatibility of electrode materials and long term stability in electrical characteristics. In this research, we prepared the primary culture of rat hippocampal neurons directly on reduced graphene oxide films which is chosen as a model electrode material for the neural electrode. We observed that the viability of primary neuronal culture on the present structure is minimally affected by nanoscale graphene flakes below. These results implicate that the multilayer films of reduced graphene oxides can be utilized for the next-generation neural interfaces with decent biocompatibility and outstanding electrical performance.