• Title/Summary/Keyword: new strain

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The nano scale buckling properties of isolated protein microtubules based on modified strain gradient theory and a new single variable trigonometric beam theory

  • Alwabli, Afaf S.;Kaci, Abdelhakim;Bellifa, Hichem;Bousahla, Abdelmoumen Anis;Tounsi, Abdelouahed;Alzahrani, Dhafer A.;Abulfaraj, Aala A.;Bourada, Fouad;Benrahou, Kouider Halim;Tounsi, Abdeldjebbar;Mahmoud, S.R.;Hussain, Muzamal
    • Advances in nano research
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    • v.10 no.1
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    • pp.15-24
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    • 2021
  • Microtubules (MTs) are the main part of the cytoskeleton in living eukaryotic cells. In this article, a mechanical model of MT buckling, considering the modified strain gradient theory, is analytically examined. The MT is assumed as a cylindrical beam and a new single variable trigonometric beam theory is developed in conjunction with a modified strain gradient model. The main benefit of the present formulation is shown in its new kinematic where we found only one unknown as the Euler-Bernoulli beam model, which is even less than the Timoshenko beam model. The governing equations are deduced by considering virtual work principle. The effectiveness of the present method is checked by comparing the obtained results with those reported by other higher shear deformation beam theory involving a higher number of unknowns. It is shown that microstructure-dependent response is more important when material length scale parameters are closer to the outer diameter of MTs. Also, it can be confirmed that influences of shear deformation become more considerable for smaller shear modulus and aspect ratios.

Characteristics and breeding of a new cultivar Pleurotus eryngii var. ferulae, 'Beesan No.2' (아위느타리 신품종 '비산2호'의 육성 및 자실체 특성)

  • Shin, Pyung-Gyun;Yoo, Young-Bok;Kong, Won-Sik;Oh, Youn-Lee
    • Journal of Mushroom
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    • v.12 no.1
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    • pp.58-62
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    • 2014
  • To develop a new cultivar of King oyster mushroom white variety (Pleurotus ferulae), GW10-68 as parental strain was selected by the method of Di-mon crossing between monokaryotic strain ASI 2798-24 derived from ASI 2798 and dikaryotic strain ASI 2803. The GW10-95(ASI 2803 ${\times}$ ASI 2798-24) was shown the best cultural characteristics, selected to be a new cultivar and designed as 'Beesan No.2'. The 'Beesan No.2' was formed incompatibility line distinctly in the confrontation growth of parental strains ASI 2803, ASI 2798 and Beesan No.1. Analysis of the genetic characteristics of the new cultivar 'Beesan No.2' showed a different DNA profile as that of the control strains, ASI 2803, ASI 2798 and 'Beesan No.1', when RAPD(Random Amplified Polymorphic DNA) primer URP4 was used. The optimum temperature and pH arrange for mycelial growth were $25^{\circ}C$ and pH5~8, respectively. Fruiting body production per bottle was about 123.3 g. And also the stipe was long. This new cultivar 'Beesan No.2' of Pleurotus ferulae was characterized white-like variety of King oyster mushroom in the color of stipe, that was long and low yield compared to that of other cultuvar 'Beesan No.1'. We therefore expect that this new strain will increase of export and substitute cultivar of King oyster mushroom.

Characteristics and breeding of a new cultivar Pleurotus eryngii var. ferulae, 'Beesan No.1' (아위느타리 신품종 '비산1호'의 육성 및 자실체 특성)

  • Shin, Pyung-Gyun;Yoo, Young-Bok;Kong, Won-Sik;Oh, Youn-Lee
    • Journal of Mushroom
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    • v.12 no.1
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    • pp.52-57
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    • 2014
  • To develop a new cultivar of King oyster mushroom white variety (Pleurotus ferulae), GW10-95 as parental strain was selected by the method of Di-mon crossing between monokaryotic strain ASI 2850-24 derived from ASI 2850 and dikaryotic strain ASI 2803. The GW10-95(ASI 2803 ${\times}$ ASI 2850-24) was shown the best cultural characteristics, selected to be a new cultivar and designed as 'Beesan No.1'. The 'Beesan No.1' was formed incompatibility line distinctly in the confrontation growth of parental strains ASI 2803 and ASI 2850. Analysis of the genetic characteristics of the new cultivar 'Beesan No.1' showed a different DNA profile as that of the control strains, ASI 2803 and ASI 2850, when RAPD(Random Amplified Polymorphic DNA) primer URP6 was used. The optimum temperature and pH arrange for mycelial growth were $25^{\circ}C$ and pH5~8, respectively. Fruiting body production per bottle was about 245 g using demonstration farms. And also the stipe was thick and long. This new cultivar 'Beesan No.1' of Pleurotus ferulae was characterized by fast fruitbody formation, the stipe was thick and long and high quality yield compared to that of other cultuvars. We therefore expect that this new strain will increase of farmer's income by construction of stabilized production system.

Characteristics and breeding of a new cultivar Pleurotus eryngii, Seolsong (큰느타리버섯 신품종 '설송'의 육성 및 그 특성)

  • Shin, Pyung-Gyun;Yoo, Young-Bok;Kong, Won-Sik;Jang, Kab-Yeul;Oh, Youn-Lee;Cheong, Jong-Chun
    • Journal of Mushroom
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    • v.11 no.2
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    • pp.77-81
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    • 2013
  • To develop a new cultivar of king oyster mushroom(Pleurotus eryngii), G09-21 as parental strain was selected by the method of Di-mon crossing between monokaryotic strain ASI 2824-21 derived from ASI 2824(Keunneutari No. 2) and dikaryotic strain ASI 2887(Aeryni 3). The Pe21-53(G09-21-10 x ASI 2844-9) was shown the best cultural characteristics, selected to be a new cultivar and designated as 'Seolsong'. The 'Seolsong' was distinctly formed incompatibility line in the confrontation growth of parental strains Keunneutari No. 2, Aeryni 3 and ASI 2844. Analysis of the genetic characteristics of the new cultivar 'Seolsong' showed a different DNA profile as that of the control strains, Keunneutari No. 2, Aeryni 3 and ASI 2844, when RAPD(Random Amplified Polymorphic DNA) primer URP4 was used. The optimum temperature and pH arrange for mycelial growth were $25{\sim}30^{\circ}C$ and pH 5~8, respectively. This new cultivar 'Seolsong' of fruiting body production per bottle was about $131.4{{\pm}}43.1$ g which is about 102% quantity compared to that of other cultivar Keunneutari No. 2. And also the stipe is thick and long, but the number of available stipe is few. Particularly, it was tolerant of high moisture above 90% during the growth period after primodia formation. We therefore expect that this new strain will save of labor and cost of cultivation by without culling work.

Attenuation and Protective Effects of a Thermostable Newcastle Disease Virus Isolated from Korean Pheasants (한국산 꿩으로부터 분리한 열 안정성 뉴캣슬병 바이러스의 순화와 방어효과)

  • 한수철;곽길한;김태중;장경수;전무형;송희종
    • Korean Journal of Poultry Science
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    • v.27 no.2
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    • pp.109-114
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    • 2000
  • The objective of these experiments was to develop an attenuated thermostable Newcastle disease virus(NDV), CBP-1 strain isolated from infected pheasants. Safety, pathogenicity and protective effects against velogenic NDV were also investigated to evaluate if the attenuated NDV, CBP-1 strain could be a candidate for a new NDV vaccine strain. CBP-1 strain was passaged up to the 173 times by nine days old embryonated eggs and chicken embryo fibroblast(CEF) cell cultures. Its sensitivitly to lipid solvents and low pH, thermostability, mean death time(MDT), intracerebral pathogenicity index(ICPI) of one day old chicks and intravenous pathogenicity index(IVPI) of four weeks old chicks were examined. Safety, boosting and protective effects were tested by chicks mortality. CBP-1 NDV strain had significant thermostability at 56$\^{C}$ for 30 minutes. by hemagglutinin activity and egg infectivity test, but was not resistant to lipid solvent. It showed possibility to use as a feed or water vaccine because of the resistance to low pH. MDT, ICPI and IVPI of CBP-1 were attenuated from 51.5, 1.96, 2.60 to 112.4, 1.12, 1.45. These results implied that the 173rd passages in embryonated egg and CEF cell cultures induced a substantial attenuation of the pathogenicity of the parent virus, changing the virulence from velogenic to intermediate between mesogenic and lentogenic. After vaccination with CBP-1 at one day old by drinking water mortality was 17.5%. However, spray vaccination with B1 at one day old, CBP-1 at two weeks ild and challenge with velogenic Kyojeongwon strain at four weeks old showed 93.5% survival rate. Mortality of chicks, vaccination with 173rd passaged CBP-1 strain at one day old, two weeks old and challenge with Kyokeongwon strain at four weeks old, was 20.0%. The results of these studies indicated that partial attenuated CBP-1 strain tended to be a low safety for ND of broiler chicks and would need to be more successive attenuation.

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Friction Stir Welding Analysis Based on Equivalent Strain Method using Neural Networks

  • Kang, Sung-Wook;Jang, Beom-Seon
    • Journal of Ocean Engineering and Technology
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    • v.28 no.5
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    • pp.452-465
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    • 2014
  • The application of friction stir welding (FSW) technology has been extended to all industries, including shipbuilding. A heat transfer analysis evaluates the weldability of a welded work piece, and elasto-plastic analysis predicts the residual stress and deformation after welding. A thermal elasto-plastic analysis based on the heat transfer analysis results is most frequently used today. However, its application to large objects such as offshore structures and hulls is impractical owing to its long computational time. This paper proposes a new method, namely an equivalent strain method using the inherent strain, to overcome the disadvantages of the extended analysis time. In the present study, a residual stress analysis of FSW was performed using this equivalent strain method. Additionally, in order to reflect the external constraints in FSW, the reaction force was predicted using a neural network, Finally, the approach was verified by comparing the experimental results and thermal elasto-plastic analysis results for the calculated residual stress distribution.

The ground response curve of underwater tunnels, excavated in a strain-softening rock mass

  • Fahimifar, Ahmad;Ghadami, Hamed;Ahmadvand, Masoud
    • Geomechanics and Engineering
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    • v.8 no.3
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    • pp.323-359
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    • 2015
  • This paper presents an elasto-plastic model for determination of the ground response curve of a circular underwater tunnel excavated in elastic-strain softening rock mass compatible with a nonlinear Hoek-Brown yield criterion. The finite difference method (FDM) was used to propose a new solution to calculate pore water pressure, stress, and strain distributions on periphery of circular tunnels in axisymmetric and plain strain conditions. In the proposed solution, a modified non-radial flow pattern, for the hydraulic analysis, is utilized. To evaluate the effect of gravitational loads and variations of pore water pressure, the equations concerning different directions around the tunnel (crown, wall, and floor) are derived. Regarding the strain-softening behavior of the rock mass, the stepwise method is executed for the plastic zone in which parameters of strength, dilatancy, stresses, strains, and deformation are different from their elasto-plastic boundary values as compared to the tunnel boundary values. Besides, the analytical equations are developed for the elastic zone. The accuracy and application of the proposed method is demonstrated by a number of examples. The results present the effects of seepage body forces, gravitational loads and dilatancy angle on ground response curve appropriately.

Broad and stage-based sensing function of HCFRP sensors

  • Wu, Z.S.;Yang, C.Q.
    • Smart Structures and Systems
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    • v.3 no.2
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    • pp.133-146
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    • 2007
  • This paper addresses a new type of broad and stage-based hybrid carbon fiber reinforced polymer (HCFRP) sensor that is suitable for the sensing of infrastructures. The HCFRP sensors, a type of composite sensor, are fabricated with three types of carbon tows of different strength and moduli. For all of the specimens, the active materials are carbon tows by virtue of their electrical conductivity and piezoresistivity. The measurement principles are based on the micro- and macro-fractures of different types of carbon tows. A series of experiments are carried out to investigate the sensing performances of the HCFRP sensors. The main variables include the stack order and volume fractions of different types of carbon tows. It is shown that the change in electrical resistance is in direct proportion to the strain/load in low strain ranges. However, the fractional change in electrical resistance (${\Delta}R/R_0$) is smaller than 2% prior to the macrofractures of carbon tows. In order to improve the resistance changes, measures are taken that can enhance the values of ${\Delta}R/R_0$ by more than 2 times during low strain ranges. In high strain ranges, the electrical resistance changes markedly with strain/load in a step-wise manner due to the gradual ruptures of different types of carbon tows at different strain amplitudes. The values of ${\Delta}R/R_0$ due to the fracture of high modulus carbon tows are larger than 36%. Thus, it is demonstrated that the HCFRP sensors have a broad and stage-based sensing capability.

Construction of The Mac Wave Detection System using Strain Gage (스트레인 게이지를 이용한 맥파 검출 시스템의 구성)

  • Yi, Chong-Ho
    • Journal of the Korean Institute of Telematics and Electronics T
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    • v.36T no.4
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    • pp.88-93
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    • 1999
  • In this paper, a new Mac-wave detection system by strain gage is constructed for study on a Mac-wave which is major factor of diagnosis in oriental medicine. Inherent resistance of a strain gage is linearly changed with strain of the sensor, thus the Mac-wave of wrist can be detected by using of the strain gage. A precision bridge circuit and high sensitive amplifier is designed for detecting the change of resistance of the strain gage in this experiments. A very small change of Mac-wave is acquired in the circuit. And the digital signal of the detected Mac-wave are acquired on a PC by multichannel A/D converter and displayed graphically by programming. The characteristics of the Mac-wave of sampled 6 persons in the time and the frequency domain are analysed, therefore the usefulness of the system constructed in this experiments are proved.

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Constitutive Modeling of Confined Concrete under Concentric Loading

  • Lee, Cha-Don;Park, Ki-Bong;Cha, Jun-Sil
    • KCI Concrete Journal
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    • v.13 no.1
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    • pp.69-78
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    • 2001
  • The inelastic behavior of a reinforced concrete columns is influenced by a number of factors : 1) level of axial load, 2) tie spacing, 3) volumetric ratio of lateral steel, 4) concrete strength, 5) distribution of longitudinal steel, 6) strength of lateral steel, 7) cover thickness, 8) configuration of lateral steel, 9) strain gradient, 10) strain rate, 11) the effectively confined concrete core area, and 12) amount of longitudinal steel. A new constitutive model of a confined concrete is suggested in order to investigate the nonlinear behavior of the reinforced concrete columns under concentric loading. The developed constitutive model for the confined concrete takes into account the effects of effectively confined area as well as the horizontal and longitudinal distributions of the confining pressures. None of the existing models incorporated these two main effects at the same time. A total of different six constitutive models for the behavior of the confined concrete under concentric compression were compared with the sixty-one test results reported by different researchers. The superiority of the developed model in its accuracy is demonstrated by evaluating the error function, which compares the weighted averages for the sum of squared relative differences in peak compressive strength and corresponding strain, stress at strain equal to 0.015, and total area under stress-strain curve up to strain equal to 0.015.

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