• ALGAE
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• v.37 no.2
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• pp.105-121
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• 2022

Modern seaweed farming relies heavily on seedlings from natural beds or vegetative cuttings from previous harvests. However, this farming method has some disadvantages, such as physiological variation in the seed stock and decreased genetic variability, which reduces the growth rate, carrageenan yield, and gel strength of the seaweeds. A new method of seedling production that is sustainable, scalable, and produces a large number of high-quality plantlets is needed to support the seaweed farming industry. Recent use of tissue culture and micropropagation techniques in eucheumatoid seaweed production has yielded promising results in increasing seed supply and growing uniform seedlings in large numbers in a shorter time. Several seaweed species have been successfully cultured and regenerated into new plantlets in laboratories using direct regeneration, callus culture, and protoplast culture. The use of biostimulants and plant growth regulators in culture media increases the seedling quality even further. Seedlings produced by micropropagation grew faster and had better biochemical properties than conventionally cultivated seedlings. Before being transferred to a land-based grow-out system or ocean nets for farming, tissue-cultured seedlings were recommended to undergo an acclimatization process to increase their survival rate. Regular monitoring is needed to prevent disease and pest infestations and grazing by herbivorous fish and turtles during the farming process. The current review discusses recent techniques for producing eucheumatoid and other valuable seaweed farming materials, emphasizing the efficiency of micropropagation and the transition from laboratory culture to cultivation in land-based or open-sea grow-out systems to elucidate optimal conditions for sustainable seaweed production.

• KSBB Journal
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• v.29 no.5
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• pp.361-371
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• 2014

Mycoplasma is well recognized as one of the most prevalent and serious microbial contaminants of biologic manufacturing processes. Conventional methods for mycoplasma testing, direct culture method and indirect indicator cell culture method, are lengthy, costly and less sensitive to noncultivable species. In this report, we describe a new TaqMan probe-based real-time PCR method for rapid and quantitative detection of mycoplasma contamination during manufacture of biologics. Universal mycoplasma primers were used for mycoplasma PCR and mycoplasma DNA was quantified by use of a specific TaqMan probe. Specificity, sensitivity, and robustness of the real-time PCR method was validated according to the European Pharmacopoeia. The validation results met required criteria to justify its use as a replacement for the culture method. The established real-time PCR assay was successfully applied to the detection of mycoplasma from human keratinocyte and mesenchymal stem cell as well as Vero cell lines artificially infected with mycoplasma. The overall results indicated that this rapid, specific, sensitive, and robust assay can be reliably used for quantitative detection of mycoplasma contamination during manufacture of biologics.

• The Research Journal of the Costume Culture
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• v.18 no.6
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• pp.1242-1253
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• 2010

The purpose of this study is to suggest a designing method with excellent body suitability by analyzing clothingwearing porosity, comparing and analyzing patterns, and evaluating outer appearances by designing a prototype of study applied with design measurements for categories that have influence on the outer appearance of clothing through diverse research methods, by comparing and analyzing bunka and new bunka style basic pattern, which are proportional basic patterns most frequently used in schools for educational use to design bodice pattern for elderly women in their late 60s. As for the process of this study, the first stage selected study subjects, measured bodies. The second stage compared patterns of bunka and new bunka style basic pattern and evaluated outer appearances that deduced important pattern designing categories to design upper-torso bodice pattern of elderly women. The results of this study are as following ; As a result of comparing and analyzing bunka and new bunka style basic pattern, overall evaluation of outer appearance was excellent for new bunka style basic pattern, and bunka basic pattern received better reviews for the width and depth of front neck. In terms of waist circumference, waist front length, bust point location and bust point-bust point, bunka and new bunka style basic patterns showed a significant difference. new bunka style basic pattern had 4.5cm larger front armhole depth than bunka basic pattern, so it was evaluated to have more appropriate front armhole length, bust point location, and bust point-bust point. Through observation for primary excellent categories and precedent studies, 7 categories of front neck width, front neck depth, front interscye, back neck width, back neck depth, back interscye, and back armhole depth were deduced to have relations to the extra room around arm holes and waist that affect pattern designing method and porosity and evaluations of outer appearance.

• Journal of the Korean housing association
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• v.18 no.2
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• pp.121-128
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• 2007

The investigation of the apartment housing as a dwelling type has become the most important and popular research subject to understand the housing culture in Korea. In their methods of typological analysis, it is found that most studies represent each unit plan as a simplified architectural drawing. This type of typology, however, has difficulties in processing a large scale of data set because each representation of a plan contain too many informations. To deal with this problem, this study suggests a new graph-theoretical method by which apartment plans can be represented in a more simple and effective way. This new method is also tested against the sample plans from Gangnam-gu area in Seoul to reveal the design logic hidden in plan configuration. Through a series of analyses, it is verified that there exist a design strategy that guides the particular pattern of zoning and allocation of each room in the plan.

• Journal of the Korean Society of Food Culture
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• v.29 no.4
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• pp.314-325
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• 2014

To contribute to the globalization of Korean food, a Korean food culture publicity event was conducted at Hayabusa Station, Tottori Province, Japan. This study investigated and analyzed recognition and preferences towards Korean food in participants at the event. The method of information acquisition was also analyzed. Most participants had prior experience eating Korean food. As for information, participants responded that they were affected by public media such as dramas, and the most effective way of getting information was participating in lectures. This study also investigated intake of 20 kinds of Korean food and found highest preferences for bibimbap, kimchi, naengmyeon, and galbigui, in that order. The main motive for participating in the Korean food culture publicity event was a desire to experience a new culture. Further, satisfaction, intention to participate, and intention to recommend Korean food were high. These attitudes had significant effects on the intention to visit Korea. In the future, Korean food culture publicity events held in foreign lands can contribute to Korean tourism.

• Journal of Korean Academy of Nursing
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• v.37 no.7
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• pp.1212-1222
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• 2007

Purpose: The present study was done to discover the structure of universal actual experiences 'Considering tomorrow' of health and quality of life among North Korean refugees in terms of the socio-cultural context of South Korea. Method: The research question was 'what is the structure of the actual experience of 'Considering tomorrow?', which was examined based on the Parse's human becoming research method. Five North Korean adult refugees were recruited from a National Reconciliation Committee in Seoul/Incheon. The data was gathered from dialogues and collected from February, 2006 to November, 2006. Results: The structures found in this study were: 'hope for future life by taking responsibility and having harmony with South Koreans, by forming an integrated identification; having a chance for positive engagement, by attaining human freedom and hope; feeling respected, by assimilating self to the new world; getting freedom back, by facing a new challenge and preparing self for a new social role; overcoming cultural differences with fortified hardiness for survival, by making a decision for a life course with individual growth. In addition, conceptual integration was that 'Considering tomorrow is transforming the enabling-limiting values'. Conclusion: Health professionals need to know North Korean refugees' psychological difficulties, expectations of treatment, help seeking behavior, and expectations from mainstream culture. Additionally, understanding North Korean refugees' needs for reality, health education and a multi-disciplinary team approach are necessary to improve their health.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.21 no.1
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• pp.1-18
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• 1995

We had tested the effect of amino propane sulfonic acid(APSA) on the human skin with non-invasive technique. It was tested with four parameters that were hydration, elasticity, color and skin wrinkles. In vitro culture systems, APSA stimulated the proliferation of fibroblasts bolt it didn't stimulate that of keratinocyte. Also we obtained the similar effects in the raft culture method. So we concluded that APSA affected the dermal region than the epidermal region. In clinical tests, APSA changed the skin color, pbiomechanical properties(especially elasticity) and reduced skin wrinkles of the volunteers. And we could get the better results of skin wrinkle improvement by use of Skin Visiometer than Silflo Image analysis systems.

• Journal of the Korean BIBLIA Society for library and Information Science
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• v.31 no.2
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• pp.203-221
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• 2020

This study closely analyzed changes in the educational environment and changes in the needs of children's and young people's reading culture programs, which are directly beneficiaries of the promotion of reading culture as they enter the fourth industrial revolution. It also comprehensively evaluated the reading culture promotion project for children and adolescents promoted by the National Children and Youth Library and proposed a reading culture promotion project that meets the needs of the smart era. This study investigated the cases of various domestic and foreign reading culture promotion projects to divulge trends. The authors invited experts from public libraries and school libraries with experience of the reading culture promotion projects and performed Focus Group Interviews (FGI). The authors evaluated individual reading culture program based on the PDCA method (Plan, Do, Check, Act). Based on the data obtained through case studies and expert evaluations, the development plan of reading culture promotion project and the strategy of promoting new projects to be pursued in the National Children and Youth Library were presented. By gathering the results of the research, 'Interactive e-book making platform production / distribution business', 'Game-type reading program production / distribution business', 'Habruta reading culture dissemination project using backward learning method', 'Youth coding branding "Teen-Start -Up"' were proposed as new services.

• The Research Journal of the Costume Culture
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• v.5 no.3
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• pp.176-183
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• 1997

To develop the bodice pattern of the master size of the established size chart which was suggested in the former studies, the wearing experiment was practiced. According to the result, developed pattern design in this study boys\` bodily characteristics were reflected fully. And especially, New pattern design method was suggested at chest circumference, neck circumference, arm circumference, chest breadth, and center front line. The study results were put into the database in order to apply apparel CAD system, and they could be easily used in standardization work of design pattern.

• International Journal of Advanced Culture Technology
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• v.10 no.4
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• pp.361-368
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• 2022

This study seeks to determine the research trend of late adolescents by utilizing big data. Also, seek for research trends related to activity participation, treatment, and mediation to provide academic implications. For this process, gathered 1.000 academic papers and used TF-IDF analysis method, and the topic modeling based on co-occurrence word network analysis method LDA (Latent Dirichlet Allocation) to analyze. In conclusion this study conducted analysis of activity participation, treatment, and mediation of late adolescents by TF-IDF analysis method, co-occurrence word network analysis method, and topic modeling analysis based on LDA(Latent Dirichlet Allocation). The results were proposed through visualization, and carries significance as this study analyzed activity, treatment, mediation factors of late adolescents, and provides new analysis methods to figure out the basic materials of activity participation trends, treatment, and mediation of late adolescents.