• 디자인학연구
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• 제11권1호
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• pp.173-184
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• 1998

본 연구는 미래 도시경관 ·건축환경에서 요구되는 미학적 질에 관한 연구로 다수가 선호하고 즐거워할 수 있는 과학적이고 객관적인 미적 가치, 시각적 특성들을 제안하는 것이 목표이다. 연구의 이론적 접근방법은 경험 과학적 미학으로 이를 통한 인간지각.인지.행동의 본성을 이해하고자 하였으며 미적 환경이 갖는 시각적 질의 특성을 범주화하고 그 효과를 설명, 예측하고자 하였다. 그리고 환경지각.인지이론과 새롭게 제시된 환경미학모델들을 검토하였으며 환경디자인에 적용될 수 있는 미학적 특성들을 제안하였다. 첫째, 시각적 질에 대한 미적 경험은 심리 생물학적 특성과 인간활동의 결합과정으로 환경의 형식적.상징적 그리고 내적인 표상체계의 상호작용에 의해서 이루어짐을 알 수 있다. 또한 시각적 질은 사회 문화적 가치, 개인적.집단적 가치 등으로 인해 다양성과 차이가 있으므로 절대화 할 수 없는 복잡한 영역으로 간주되었다. 둘째, 시각적 질의 효과, 미적 반응은 궁극적으로 즐거움, 선호, 인간생존을 위한 적응과 자율성 등에 관계된다. 셋째, 미학은 더욱더 통합적인 학제간 연구를 통해 발전될 수 있으며 이러한 미학적 경험자료는 디자인, 계획, 평가를 위한 기초적인 정보가 될 수 있을 것으로 판단된다.

• 대한치과보존학회:학술대회논문집
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• 대한치과보존학회 2001년도 춘계학술대회
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• pp.235-237
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• 2001

;Dentistry has benefited from tremendous advances in technology with the introduction of new techniques and materials, and patients are aware that esthetic approaches in dentistry can change one's appearance. Increasingly. tooth-colored restorative materials have been used for restoration of posterior teeth. Tooth-colored restoration for posterior teeth can be divided into three categories: 1) the direct techniques that can be made in a single appointment and are an intraoral procedure utilizing composites: 2) the semidirect techniques that require both an intraoral and an extraoral procedure and are luted chairside utilizing composites: and 3) the indirect techniques that require several appointments and the expertise of a dental technician working with either composites or ceramics. But, resin restoration has inherent drawbacks of microleakage. polymerization shrinkage, thermal cycling problems. and wear in stress-bearing areas. On the other hand, Ceramic restorations have many advantages over resin restorations. Ceramic inlays are reported to have less leakage than resin restoration and to fit better. although marginal fidelity depends on technique and is laboratory dependent. Adhesion of luting resin is more reliable and durable to etched ceramic material than to treated resin composite. In view of color matching, periodontal health. resistance to abrasion, ceramic restoration is superior to resin restorationl. Materials which have been used for the fabrication of ceramic restorations are various. Conventional powder slurry ceramics are also available. Castable ceramics are produced by centrifugal casting of heat-treated glass ceramics. and machinable ceramics are feldspathic porcelains or cast glass ceramics which are milled using a CAD/CAM apparatus to produce inlays (for example, Cered. They may also be copy milled using the Celay apparatus. Pressable ceramics are produced from feldspathic porcelain which is supplied in ingot form and heated and moulded under pressure to produce a restoration. Infiltrated ceramics are another class of material which are available for use as ceramic inlays. An example is $In-Ceram^{\circledR}$(Vident. California, USA) which consists of a porous aluminum oxide or spinell core infiltrated with glass and subsequently veneered with feldspathic porcelain. In the 1980s. the development of compatible refractory materials made fabrication easier. and the development of adhesive resin cements greatly improved clinical success rates. This case report presents esthetic ceramic inlays for posterior teeth.teeth.

• Advances in Traditional Medicine
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• 제5권3호
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• pp.216-230
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• 2005

Although the field of study in immune enhancing compounds is relatively new, natural products from plants represent a rich and promising source of novel molecules with immunomodulating properties, Microglial cells, the main immune effector cells of the brain, usually display a ramified morphology and low expression levels of immunologically relevant antigens such as MHC class I and class II. Since any compound which participates in activation of phagocytic cells contributes to the production of potentially toxic factors, the search for convenient in vitro test-systems and study of mechanisms of action of these agents are of great interest. Human blood polymorphonuclear (PMN) cells and primary microglial cells isolated from Sprague-Dawley rats were used as cellular screening tests for study of phagocytosis-stimulating action of immunomodulating agents. Numbers of phagocytic activity were evaluated by the phagocyte ingestion of yeast cells and NO-synthase activity, nitrite production, and nitroblue tetrazolium test were determined after phagocyte stimulation. It was possible to demonstrate that indexes of phagocytic activity can be used as quantitative indicators for measurement immunomodulating activity. As a positive control, Zymosan A-induced phagocytosis in both PMN cells and primary microglial cells was used. $IFN-{\gamma}$ (0.1 -1 U/ml) stimulated phagocytosis in PMN cells 1.2 times after 2 - 3 h incubation, although at higher concentrations (10 - 100 U/ml) it strongly inhibited phagocytosis. In a similar way, at higher concentrations, $IFN-{\gamma}$ (100 - 500 U/ml) suppressed phagocytosis in zymosan-A stimulated microglial cells. When Polypodium leucotomus, cambricum and vulgare extracts were tested alone, increased levels of phagocytosis were observed in PMN. In addition, microglial cells showed both increased phagocytosis and MHC class-II antigen expressions. Surprisingly, when PMN and microglia were treated with a combination of Polypodium and $IFN-{\gamma}$, phagocytosis was not inhibited. We did not find changes in NO-synthase activity and nitrite production in both microglia and PMN cells activated by different immunomodulating agents. These results indicate that primary microglial cell cultures as well as human PMN cells can provide reproducible quantitative results in screening phagocytic activity of different immunoactive compounds. Furthermore, both inhibitory or activation mechanisms might be studied using these in vitro experimental approaches.

• Journal of Ginseng Research
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• 제38권4호
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• pp.270-277
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• 2014

Background: The effect of methyl jasmonate (MJ) on ginsenoside production in different organs of ginseng (Panax ginseng Meyer) was evaluated after the whole plant was dipped in an MJ-containing solution. MJ can induce the production of antioxidant defense genes and secondary metabolites in plants. In ginseng, MJ treatment in adventitious root resulted in the increase of dammarenediol synthase expression but a decrease of cycloartenol synthase expression, thereby enhancing ginsenoside biosynthesis. Although a previous study focused on the application of MJ to affect ginsenoside production in adventitious roots, we conducted our research on entire plants by evaluating the effect of exogenous MJ on ginsenoside production with the aim of obtaining new approaches to study ginsenoside biosynthesis response to MJ in vivo. Methods: Different parts of MJ-treated ginseng plants were analyzed for ginsenoside contents (fine root, root body, epidermis, rhizome, stem, and leaf) by high-performance liquid chromatography. Results: The total ginsenoside content of the ginseng root significantly increased after 2 d of MJ treatment compared with the control not subjected to MJ. Our results revealed that MJ treatment enhances ginsenoside production not in the epidermis but in the stele of the ginseng root, implying transportation of ginsenosides from the root vasculature to the epidermis. Application of MJ enhanced protopanaxadiol (PPD)-type ginsenosides, whereas chilling treatment induced protopanaxatriol (PPT)-type ginsenosides. Conclusion: These findings indicate that the production of PPD-type and PPT-type ginsenosides is differently affected by abiotic and biotic stresses in the ginseng plant, and they might play different defense mechanism roles.

• 교육시설 논문지
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• 제25권1호
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• pp.13-24
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• 2018

Planning and designing training facilities has been developed from educational facilities, and increasingly diversified society has raised a need for facilities dedicated to training and education in a differentiated space separate from the one for regular works. This particular need of our times has led to the expansion of training facilities nationwide although they have something to be desired when it comes to planning and designing with sustainability associated with the locational characteristics of urban space as well as the unique types of facilities taken into account. Against this backdrop, this study will examined a variety of training facilities that have been established since 2000 through theoretical review and conduct intensive analysis on the characteristics of the planning aspects to suggest their significance and implications and to present the overall meanings and ramifications of planning approaches in consideration of new challenges modern training facilities are faced with, which have been revealed through architectural design competitions in recent years. The relevant implications are as follows. First, one of the locational advantages of training facilities, which is commanding beautiful scenery of the surrounding area, can be considered as intent to stress the aspect of a resort, one of the functions of any training facilities. As this study has demonstrated, many training facilities are located near around a beach or a lake. Second, training facilities can be classified into three different types in terms of their location: urban, suburban and resort and such locational characteristics are directly related to intended programs and differentiated links with target users. Third, the architectural styles of training institutes are differentiated in terms of harmonious arrangement between beautiful natural scenery and buildings in consideration of the layout characteristics of major facilities and the distance of ramps in and out of the facilities along with architectural features, including the transparency of building elevation and the type of slopes of roof structure. Fourth, the individual lodging buildings feature a variety of types depending on the design concept with different roles depending on the directional aspects such as the connection of ramps and the relations with the outside. Fifth, outdoor space plans are differentiated according to the intended purpose of training facilities. When it comes to gym facilities, for example, different outdoor space plans are found to be made depending on the original design concept such as outdoor playground-centered planning or golf facilities.

• 한국응용과학기술학회지
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• 제33권1호
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• pp.118-128
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• 2016

대기오염에 관한 관심은 국내 외에서 점진적으로 상승하고 있으며, 자동차와 연료 연구자들은 청정(친환경 대체연료) 연료와 연료품질 향상 등을 이용하여 새로운 엔진 설계, 혁신적인 후 처리 시스템 등의 많은 접근을 통하여 차량 유해 배기가스를 감소시키려고 노력하고 있다. 이러한 연구들은 가솔린 자동차의 배출가스 및 가솔린 차량의 PM 입자 배출 등의 두 가지 이슈로 진행되고 있다. 자동차의 배출가스 및 PM(입자상 물질) 입자는 환경오염과 인체에 악영향을 주는 많은 문제를 일으키고 있다. 추가로, 함산소 첨가제로서 연료에 포함된 MTBE (Methyl Tertiary Butyl Ether)에 대한 환경 문제점을 연구하고 있다. 연구자들은 MTBE가 건강에 미치는 영향에 대한 많은 데이터를 가지고 있다. 이러한 데이터는 높은 MTBE 용량에서 잠재적인 발암 물질 임을 결론짓고 있다. 함산소 연료첨가제 유형 (MTBE, 바이오 ETBE, 바이오 에탄올, 바이오 부탄올)에 기초하여, 본 논문은 가솔린 연료 물성 및 증발가스 배출 특성에 대해 산소함량의 영향을 검토하였다. 또한, 연료물성에 대한 휘발유 차량의 가속 및 출력 성능을 평가하였다.

• Bulletin of the Korean Chemical Society
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• 제30권5호
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• pp.1152-1156
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• 2009

Silent information regulator 2 (Sir2) or sirtuins are NAD(+)-dependent deacetylases, which hydrolyze the acetyllysine residues. In mammals, sirtuins are classified into seven different classes (SIRT1-7). SIRT1 was reported to be involved in age related disorders like obesity, metabolic syndrome, type II diabetes mellitus and Parkinson’s disease. Activation of SIRT1 is one of the promising approaches to treat these age related diseases. In this study, we have used HipHop module of CATALYST to identify a series of pharmacophore models to screen SIRT1 enhancing molecules. Three molecules from Sirtris Pharmaceuticals were selected as training set and 607 sirtuin activator molecules were used as test set. Five different hypotheses were developed and then validated using the training set and the test set. The results showed that the best pharmacophore model has four features, ring aromatic, positive ionization and two hydrogen-bond acceptors. The best hypothesis from our study, Hypo2, screened high number of active molecules from the test set. Thus, we suggest that this four feature pharmacophore model could be helpful to screen novel SIRT1 activator molecules. Hypo2-virtual screening against Maybridge database reveals seven molecules, which contains all the critical features. Moreover, two new scaffolds were identified from this study. These scaffolds may be a potent lead for the SIRT1 activation.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제27권2호
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• pp.103-109
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• 2005

In spite of the ongoing advances, standard therapies for oral cancer still has some limitations in efficacy and in ability to prolong survival rate of advanced disease and result in significant functional defect and severe cosmetic deformity. Currently gene therapy using tumor suppressor gene is considered as a potent candidate for new therapeutic approaches that can improve efficacy and reduce complications. The purpose of this research is to identify the role of adenoviral vector to transfer HCCS-1 tumor suppressor gene in oral cancer cells and to find out whether there is a possibility for it to serve in the field of gene therapy. The human SCC-25 cell line was used for transfection. To determine the efficiency of the adenovirus as a gene delivery vector cell line was transduced with LacZ gene and analysed with X-gal staining. Northern blot was performed to confirm the tranfection with HSCC-1 gene and cell viability was assessed by cell cytotoxicity assay. We had successfully construct the recombinant HSCC-1 adenovirus(Ad5CMV-HCCS-1). DNA extracted from Ad5CMV-HCCS-1 revealed HCCS-1 gene is incorporated. The transduction efficiencies were over than 50% of SCC-25 cells with a MOI of 2 and over 95% with a MOI of 50. Northern blot analysis showed that a single 0.6kb mRNA transcript was expressed in Ad5CMV-HCCS-1 transduced SCC-25 cells. There was no or very low transcription HCCS-1 mRNA in wild and Ad5CMV-LacZ transduced SCC-25 cells. Cells transduced with Ad5CMV-HCCS-1 showed significant growth inhibition. By day 6, Ad5CMV-HCCS-1 treated cell count was decreased to 30% of mock-infected cells, while that of Ad5CMV-LacZ treated cells was 90% of mock-infected cells (p<0.05). Finally, these result suggest that the Ad5CMV-HCCS-1 has potential as a gene therapy tool for oral cancer.

• 대한지리학회지
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• 제44권6호
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• pp.813-832
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• 2009

농촌지역의 과소화에 따라 의료서비스에 대한 공간적 접근성이 제한되고, 노령화에 따른 의료서비스의 수요가 급격히 증가함에 따라, 최근 방문보건서비스와 같은 서비스 제공자가 수요자에게 직접 이동하는 새로운 형태의 의료서비스가 많은 주목을 받고 있다. 이러한 의료환경의 변화는 공공 의료기관의 한정된 의료자원을 효율적으로 배분하고 운영하기 위한 혁신적이고 과학적인 접근법개발에 대한 요구를 증대시키고 있다. 이러한 배경하에서 본 연구의 목적은 방문 의료서비스 제공을 위한 구역 설정 모델을 수립하고, GIS 환경에서 구역 설정 모델을 구현하는 자동구획절차(AZP) 알고리즘을 개발하는 것이다. 구역 설정 모델은 구역 내 이동성, 구역간 업무량 균형, 연속성 등의 조건을 고려한다. 이동성에 대한 세 개의 서로 다른 목적함수를 평가한다; 1) 구역 내 단위 지역간 네트워크 거리의 합 최소화, 2) 구역 내 단위 지역간 공간상호작용 최대화, 3) 구역 내 단위 지역을 순회하는 경로의 길이 최소화. 모델을 위한 AZP는 GIS 환경에서 개발되었고, 농촌의 방문보건 사례에 적용되었다. 결과는 개발된 AZP를 업무량 균형과 연속성 제약조건하에서 각 목적함수에 대하여 상이한 구획체계를 산출할 수 있음을 보여준다.

• 대한지리학회지
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• 제51권5호
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• pp.691-711
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• 2016

경제활동공간이 역동적으로 변화하고 있다. 특히 서울시 성동구 성수동 지역은 제조업 집적지였지만, 현재 급격한 변화를 겪고 있는 대표적인 지역 중 하나라고 할 수 있다. 성수동 지역은 도심 제조업이 쇠퇴하면서 공장 이전이나 폐업으로 인한 빈 공간에 새로운 행위자가 유입되고 있다. 이러한 행위자들은 전통적인 접근법에서는 다소 간과되었던 비경제적 행위자인 문화예술 부문의 창조적 행위자들이며, 이들의 비공식적인 모임인 문화예술 인식공동체는 생태계 형성 초기에 지역 생태계가 창조적 환경으로 변화하는데 영향을 끼친 것으로 보인다. 본 연구의 목적은 도시 공간구조 변화 메커니즘의 근본적인 동인을 규명하여 공간적 함의를 도출하고자 하는 것이며, 이를 위해 진화적인 관점에서 인식공동체의 단계별 형성과정에 따라 초기 문화예술 기반의 창조적인 지식생태계의 형성과정과 추동요인을 고찰하고자 하였다. 그 결과, 인식공동체는 진화적인 경로 창출과정에서 지역 프로젝트를 진행하며 지식창출을 위한 지식플랫폼으로서 일시적인 공간 형성에 기여했고, 성수동 지역은 공간적 근접성을 넘어 관계적 근접성을 추구하며 관계적 공간을 형성하고 있는 것으로 나타났다.