• Title/Summary/Keyword: nematodes

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Seasonal changes in pasture populations of infective larvae of gastrointestinal nematodes of sheep (목초(牧草)에 나타나는 면양위장(緬羊胃腸)내 선충류(線蟲類) 유충(幼蟲)의 시기(時期)적 변화(變化))

  • Suh, Guk-hyun;Lee, Chung-gil;Park, Young-jun;Lee, Chai-young
    • Korean Journal of Veterinary Research
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    • v.30 no.4
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    • pp.499-505
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    • 1990
  • The seasonal availability and abundance of the free-living stages of sheep nematodes is a key factor in the occurrence and severity of parasitic infection, and studies of larvae ecology could result in more rational control measures. In the present study seasonal pasture contamination and availability of nematodes for grazing sheep was examined as a baseline work for nematode control program at Namwon Branch, National Animal Breeding Institute during the period April 1988 through March 1989. Standard meteorological measurements were available from Unbong Sub-station, Honam Crops Experiment Station located about 200m from the experimental site. A total of 5 kinds of nematode larvae was detected: Haemonchus contortus was most prevalent (38.0%) with a decreasing order of Ostertagia spp (35.1%), Trichostrongylus spp (19.8%), nematodirus spp (6.6%), and Oesophagostomum spp (0.5%). A succession of species was recorded, in particular Ostertagia spp in May; Haemonchus contortus in June and July; Trichostrongylus spp in July and August; Nematodirus spp in August and September. These results can be incorporated into the nematode control program. To make a more rational control program, however, repeated herbage larval counts should be undertaken soil larval counts and fecal larval counts in the future.

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Nematodes and Insects Associated with Dead Trees, and Pine Wood Nematode Detection from the Part of Monochamus alternatus (고사목에서 분리된 선충과 곤충의 종류 및 솔수염하늘소 부위별 소나무재선충 밀도조사)

  • 이상명;추호렬;박남창;문일성;김준범
    • Korean journal of applied entomology
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    • v.29 no.1
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    • pp.14-19
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    • 1990
  • Nematodes and insects associated with dead trees were surveyed on the 238 dead trees belonging to 19 tree species in Pusan, Gyeongnam, Gyeongbuk, Chunnam, and Chonbuk provinces from April to September of 1989. Pine wood nematode, Bursaphelenchus xylophilus was found only in Pusan but B. mucronatus was collected at Chinju and Chinhae. The 13 nematode species in 9 genera were identified. Out of them, Diplogasteroides dimidius, Rhabdontolaimus adephagus, R. janae, Mikoletzkya diluta, M. ruminis, M. langcaudaa, Parasitorhabditis hylurgi, Panagrolaimus concolor, Panagrodontus dentatus, Prothalonema intermedium, and marcrolaimus canadensis were recorded for the first time in Korea. Insects collected from dead trees were 5 orders, 9 families, 25 genera, and 27 species. of them, the Coleoptera were the most collected insects by the 3 families, 19 genera and 22 species. The Scolytidae were 12 species in 10 genera. Hypothenemus eruditus was firstly collected from Campylotropis macrocarpa, Lespedeza maximowizi, Forsythia ovata, Meliosma oldhami, Securinega suffruticosa, Broussonetia kazinoki, and Cornus walteri. The maximum number of pine wood nematode was separated from the abdomen of Monchamus alternatus, the pine woodnematode vector. The maximum number of nematodes per an adult of M. alternatus was 127,535, minimum 2,616, and average 42,817.

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Survey of Plant Parasitic Nematodes on Economic Crops (경제작물(經濟作物) 주산단지(主産團地) 선충 발생상황(發生狀況) 조사(調査))

  • Cho, H.J.;Han, S.C.
    • Korean journal of applied entomology
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    • v.25 no.3 s.68
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    • pp.175-182
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    • 1986
  • A study was conducted to survey the fauna and the field density ofp lant parastic nematodes on important cash crops in Korea. Studies were carried out with sampling about five hundred grams of soil around roots of eighteen different kinds of crops from fifty five locations throughout the country. Nematodes were elutriated from over three thousand soil samples, and identified into sixteen different genera. Among them Ditylenchus species were found to be the most abundant in fields of garlic and onion, Helicoty-lenchus of sesame, chinese cabbage and mulberry, Meloidogyne of red-pepper, tomato, cucumber and peanut, Pratylenchus of mulberry and peach, Pratylenchus of apple, Chinese cabbage and radish, Trichodorus of potato, and Xiphinema of peach and potato. Field density of Meloidogyne species was increased with the longer continuous cultivation of red-pepper in the same field.

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Nematodes Associated with Forest Trees in Korea IV. Distribution and Species of Nematodes Associated with Forest Trees (한국에 있어서 림목에 기생하는 선충조사 IV. 수목 기생선충의 종류 및 분포)

  • 최영열;추호열;김영진;문일성;백현실
    • Korean journal of applied entomology
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    • v.31 no.4
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    • pp.427-451
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    • 1992
  • The nematodes associated with forest trees were surveyed in the National Parks and Mountains in Korea. A total of 73 species on 28 genera were identified from rhizosphere of 154 tree s species. Platycarya strobilacea, Ulmus davidiana and Acer ginnala were known to new host p plants of Meloinema kerongens. Bursaphelenchus xylophilus was detected only in Pusan on Pi$\pi$us t thunbergii, B. mucronatus was detected in Cheju, Masan, and Chinhae. Insect parasitic nemat todes, Prothallonema intermedium, on P. rigida in Daegu, P. thunbergii in Cheju city, Namwonu up, Gujoa-up and Aewol-up. The number of nematode species according to forest trees was t the highest on P. thunbergii to 32 species, followed by 24 species on Abies holophylla, 20 species on Zelkova serrata, 19 species on Diospyros kaki, 18 species on Quercus acutissima, respectively. L Localities of distribution according to nematode species were as follows; Crico$\pi$emella i$\pi$formis i in 81, X. americanum in 74, Tylenchorhynchus claytoni in 51, C. macrodora in 35, H Hemicriconemoides varionodus and Ogma serratum in 31, Pararotylenchus pini in 25, H Hemicycliophora koreana in 24, Aphelenchus avenae and C. pseudohercyniensis in 24, respectively.

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Resistance of Cowpea Cultivars to Meloidogyne arenaria and M. incognita in Korea (동부품종의 국내 분포 뿌리혹선충 저항성 검정)

  • Kang, Heonil;Jun, Taehwan;Kwon, Soonwook;Kim, Suntae;Kang, Hangwon;Kim, Yongchul;Kim, Donggeun;Ko, Hyoungrai;Choi, Insoo
    • Journal of Environmental Science International
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    • v.27 no.12
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    • pp.1241-1247
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    • 2018
  • This study was conducted to evaluate resistance of 10 different cowpea cultivars which were introduced from the United States against two major root-knot nematodes, Meloidogyne arenaria and M. incognita, that affect greenhouse crop cultivation in Korea. The results showed that the resistance of the tested cultivars to the root-knot nematodes varied with the cultivar. Texas Cream 40 showed moderate resistance to M. arenaria whereas the other cultivars were susceptible. Purple Hull Pinkeye and Texas Pinkeye Purple Hull were found to be resistant to M. incognita, whereas Mississippi Silver showed moderate resistance and the other cultivars were susceptible. As the cultivars exhibit resistance to M. arenaria and M. incognita, such cultivars resistant to root-knot nematodes should be considered during the cultivation of cowpea as a green manure crop.

Description of Diplotriaena manipoli (Nematoda: Diplotriaenoidea) Detected in the Body Cavity of Garrulus glandarius brandtii from Republic of Korea

  • Hong, Eui-Ju;Ryu, Si-Yun;Chae, Joon-Seok;Kim, Hyeon-Cheol;Park, Jinho;Cho, Jeong-Gon;Choi, Kyoung-Seong;Yu, Do-Hyeon;Park, Bae-Keun
    • Journal of Veterinary Clinics
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    • v.36 no.3
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    • pp.133-138
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    • 2019
  • The present study was performed to identify the nematodes recovered from the Eurasian jay, Garrulus glandarius brandtii, from Daejeon Metropolitan City, the Republic of Korea. Total five nematode worms were detected in the body cavities of two out of the twenty birds necropsied, and they were identified using morphological features, light and scanning electron microscope (SEM), and molecular (18S rRNA analysis) methods. The nematodes were all female Diplotriaena manipoli and had numerous eggs at different developmental stages in the uterus. The nematodes were long and slender measuring about 123-145 mm. The eight submedian cephalic papillae were arranged into four large, outer papillae and four small, inner-circle papillae. A pair of amphidal pores were located at the lateral portion of the mouth. The manubrium apex of trident was rounded and three branches of trident were bluntly rounded at the posterior ends. Using 18S rRNA partial sequence arrangements, DNA analysis of nematode worms was also carried out, and they were identified to be close to the Serratospiculum tendo based on a phylogenic tree analysis. To our knowledge, this is the first report on the molecular characterization and SEM study of D. manipoli.

Study on Hot Water Immersion Treatment for Control of Meloidogyne spp. and Pratylenchus spp. in a Ginger, Zingiber officinale (생강에서 뿌리혹선충과 뿌리썩이선충의 사멸을 위한 온탕침지처리 연구)

  • Cho, Donghun;Park, Kyonam;Kim, Yangho;Koh, Kyung-bong;Park, Youngjin
    • Korean journal of applied entomology
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    • v.56 no.2
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    • pp.171-177
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    • 2017
  • Plant parasitic nematodes, Meloidogyne and Pratylenchus spp., are mostly detected in imported bulbs and tubers including a ginger, Zingiber officinale in Korea by quarantine inspection. However, there is little information on hot water immersion treatment (HWIT) for control of exotic nematodes, which induce economic loss by discard or send back to exporter, in imported gingers. In here, we determined that mortality of two plant parasitic nematodes and thermal stability of ginger. Meloidogyne and Pratylenchus spp. were completely killed at $48^{\circ}C$ and $49^{\circ}C$ for 30 sec by HWIT. Thermal conduction of Z. officinale to reach a target temperature as $50^{\circ}C$ take 10~32 min and 6~16 min for core and inner 5 mm region from surface, respectively. When ginger exposed at $51^{\circ}C$ for 30 min, growth of Z. officinale was not affected by heat treatment compared with control. Based on these results, HWIT at $51^{\circ}C$ for 30 min completely killed artificially infected juveniles of Meloidogyne spp. in Z. officinale. Therefore, this condition for HWIT will be used as fundamental information on phytosanitory to kill two plant parasitic nematodes without damage on ginger.

Characteristics of the Oxygen Uptake Rate of Entomopathogenic Nematodes Steinernema spp. (곤충병원성 선충 Steinernema spp.의 산소 요구도 특성)

  • 김도완;박선호
    • Korean journal of applied entomology
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    • v.38 no.2
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    • pp.123-128
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    • 1999
  • Recently, entomopathogenic nematodes have received a considerable attention as biologicalcontrol agents. For in vitro cultivation, storage and transportation of nematodes, oxygen supply isextremely impotant due to its limited solubility and mass transfer problem. The oxygen uptake rates(OURs) of four different Steinernema species were measured in a 5L bioreactor at varying temperatures.The OURs of the Steinernema spp. were below 0.5 x mmolO'||'&'||' . min in the range of 13-17$^{\circ}$C. TheOURs (mmo102/L - min) of S. glaseri Dongrae and S. carpocapsae Pocheon strains were 0.4 x lo-', 0.75x lo-\ulcorner at 21$^{\circ}$C, 1.5 x lo-\ulcorner, 3.2 x 10-2 at 25"C, and 2.8 x lo-', 5.8 x lo-\ulcorner at 29"C, respectively. However,the OURs were not significantly altered by the agitation speed of 50-150 rpm. The specific oxygenuptake rates (qol) of S. glaseri NC, S. glaseri Dongrae, S. glaseri Mungyeong and S. carpocapsaePocheon strains were 0.3 x 0.5 x 0.3 x and 0.2 x mmolO~/cell min at 25"C,respectively. As the nematode size and temperature were increased, the qo, was also increased.the qo, was also increased.

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Effect of Turfgrass Height and Aeration on Pathogenicity of Entomopathogenic Nematodes to White Grubs in Golf Courses (골프장에서 잔디의 예고와 통기작업이 곤충병원성선충의 굼벵이 방제효과에 미치는 영향)

  • Lee Dong-Woon;Choi Woo-Geun;Lee Sang-Myeong;Choo Ho-Yul;Kweon Tae-Woong
    • Korean journal of applied entomology
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    • v.45 no.1 s.142
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    • pp.67-74
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    • 2006
  • Korean entomopathogenic nematodes were introduced into cultural management of turfgrasses to enhance white grub control in golf courses for saving labour and expenses and contribution to giving safe playing and working places for golfers and superintendents by environmentally friendly control strategy. The turfgrass height influenced efficacy of entomopathogenic nematodes. Efficacy was higher at short turfgrass both in pot using Galleria mellonella larvae and in golf courses using 2nd instar of Exomala orientalis and Ectinohoplia rufipes as baits. Aeration increased the efficacy of entomopathogenic nematodes which were lower virulent. Pre-aeration was more effective than post-aeration. Exomala orientalis larval population was reduced 80.4 and 66.0% in the pre-aeration and post-aeration with entomopathogenic nematode, Steinernema carpocapsae Pocheon strain compared to 35.4% in the no aeration treatment.

Nematodes Associated with Forest with Forest Trees in Korea I. Studies on Nematodes Associated with Saplings (한국에 있어서 임목에 기생하는 선충조사 I. 묘목기생선충에 관한 연구)

  • 최영렬;조명래;문일성
    • Korean journal of applied entomology
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    • v.31 no.1
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    • pp.50-68
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    • 1992
  • The results of survey on the nematodes associated with sapling from 8 Forestry Experiment Stations(F.E.S.) in Korea are reported herein. A total of 35 species under 20 genera, 11 families, and 2 orders were identified from the soils around the roots of 119 tree species and amongst them, Xiphinema insigne is unrecorded species for Korea and Geocenamus sp. is a new species. Tylenchorhynchus claytoni, which showed the highest detection rate (D.R.) from all F.E.S., except Nambu F.E.S, was the dominant species among the nematodes associated with saplings. The D.R. of T. claytoni was the highest in Ch’ungnam F.E.S. by 100% from 15 saplings sampled, followed by 87.5% in Kangwon, 66.6% in Kyongbuk, 58.3% in Cheonbuk, 48.1% in Ch’ungbuk, 41.3% in Kyongki, and 40% in Cheonnam F.E.S. Other important nematode species which showed very high densities by stations are; Criconemella morgensis (D.R.: 37.5%), Helicotylenchus digonicus (D.R. : 35%), Pararotylenchus pini (D.R.: 37%), Tylenchorhynchus nudus (D.R.: 37.9%), Criconemella informis (D.R.: 27.5%), and Meloidogyne sp. (D.R.: 12.5), from Chunbuk, Chunnam, Ch’ungbuk, Kyongki, and Kangwon F.E.S., respectively.

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