• Korean Journal of Fisheries and Aquatic Sciences
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• v.46 no.6
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• pp.901-906
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• 2013

We examined the availability of the lacZ gene (${\beta}$-galactosidase gene) as a reporter of foreign gene transfer in the cysts of Artemia franciscana (A. franciscana) to conduct a risk assessment of living genetically modified organisms (LMOs) in the marine ecosystem. The LacZ gene was transferred to decapsulated cysts by particle bombardment, and its insertion and expression were assessed by means of polymerase chain reaction (PCR) and X-gal staining. X-gal staining indicated lacZ expression in all A. franciscana examined (including the control group), which exhibited not only negative but also positive PCR amplification. Endogenous ${\beta}$-galactosidase is highly active in the whole body of A. franciscana during all stages of the life cycle. Thus, the lacZ gene is unsuitable as a reporter for foreign gene transfer in A. franciscana cysts, because it is difficult to discriminate between exogenous and endogenous ${\beta}$-galactosidase activity.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2006.11a
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• pp.166-167
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• 2006

Dendrimers represent a new class of synthetic macromolecules characterized by a regularly branched treelike structure. Multiple branching yields a large number of chain ends that distinguish dendrimers from conventional star-like polymers and microgels. The azobenzene dendrimer is one of the dendrimeric macromolecules that include the azo-group exhibiting a photochromic character. Due to the presence of the charge transfer element of the azo-group and its rod-shaped structure, these compounds are expected to have potential interest in electronics and photoelectronics, especially in nonlinear optics. In the present paper, we give pressure stimulation to organic thin films and detect the induced displacement current. Functional photoisometrization organic molecular the photo-stimulus to organic monomolecular L-films and LB films of dendrimer and 8A5H were performed. The 8A5H organic monolayer in case of pressure stimulus occurred that positive course but in case of the photo-stimulus compared positive and negative. It is assumed that generation forms of displacement current were measured when photo-stimulus for Impression.

• International Journal of Quality Innovation
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• v.10 no.1
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• pp.115-126
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• 2009

Video transmission effectiveness in the Ad Hoc network is becoming important recently, if different routing protocols are applied. Some researchers conclude that the reactive protocols are better for file transfer protocol (FTP) and constant bit rate (CBR) or hypertext transfer protocol (HTTP) transmission in an Ad Hoc wireless network but the performance report of video transmission is not much. This study adopts Qualnet (Network Simulator) as a simulation tool for environmental designing and performance testing, and employs an experimental design with eight groups. Our experiment shows that: (1) The performance of AODV (reactive) protocol is better than DSDV, ZRP and DSR when the transmission load has only one video stream; (2) Proactive (DSDV) and Hybrid protocols (ZRP) are better for a smaller Ad Hoc network when it transmits a video stream with some applications (VoIP, FTP and CBR). We conclude that packet loss rate is sensitive to the quality of video transmission and it has negative relationship with Peak Signal-to-Noise Ratio (PSNR) value. In addition, our experiment also shows that PSNR is a simple Metric for the performance evaluation of video transmission.

• Journal of Embryo Transfer
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• v.7 no.1
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• pp.21-30
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• 1992

This study was aimed to develope the new method for bovine sperm capacitation through testing and combinating factors relating to sperm capacitation. For verifying the efficiency of this method on inducing capacitation, in vitro fertilization was carried out. The results obtained were as follows: When pHs of HBS /PR for sperm-washing were 5.99, 6.38, 6.78, 7.10, 7.40, 7.69, 8.15, 8.45 and 8.83, variances between light absorhance differences obtained from sperm pre-washing solution and post-washing solution(VADs) were 0.000, 0.001, -0.001, -0.005, -0.005, -0.021,-0.017,-0.016,and-0.036, respectively. There were significant decreases of VADs in pH 7.69 and pH 8.83. When sperm were firstly, secondly, and thirdly washed with HBS /PR, VADs were -0. 024, - 0.006, and - 0.004, respectively. There was significant decrease of VAD in 1st sperm-washing. When washed-sperm were secondly washed with HBS /PR supplemented with no (con-trol), heparin, CSA, PC12, and BSA, VADs were 0.009, -0.024, -0.008, -0.009, and 0.014, respectively. When the sperm were thirdly washed with HBS /PR with no(control), heparin, CSA, PC12 and BSA, VADs were 0.020, -0.007, 0.005, 0.006, and 0.019, respectively. Only heparin treatment showed the negative values of VADs in 2nd and 3rd sperm-washing. Of oocytes cultured with sperm which were repeatedly washed with heparin and high pH, 52% (57/110) were cleaved over 2 cell stage. However, percent of oocytes parthenogenetically divided was 5%(2 /42).

• Journal of the Korean Society of Visualization
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• v.8 no.1
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• pp.53-60
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• 2010

Experimental investigations were conducted to study the characteristics of turbulent swirling flow in an axisymmetric annuli. Swirl angle measurements were performed using a flow visualization technique using smoke and dye liquid for Re=60,00080,000. Using the two-dimensional particle image velocimetry method, we found the time-mean velocity distribution and turbulent intensities in water with swirl for Re=20,000, 30,000, and 40,000 along longitudinal sections. Neutral points occurred for equal axial velocity at y/(R-r)=0.70.75, and the highest axial velocity was recorded near y/(R-r)=0.9. Negative axial velocity was observed near the convex tube along X/(D-d)=3~23. Another experimental study was performed to investigate heat transfer characteristics of turbulent swirling flow in an axisymmetric annuli. Static pressure, and local flow temperature were measured using tangential inlet condition and the friction factors and Nusselt number were calculated for several Reynolds numbers.

• Transactions of the Korean Society of Automotive Engineers
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• v.19 no.5
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• pp.113-117
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• 2011

The frame for automotive assembly can be deformed and remained on the residual stress due to high temperature thermal attacks when in welding. The frame deformation can be made to problems when in assembly with body and the residual stress can affect the negative effect on durability performance of the automobile. In order to analyze the frame deformation, the simplified test frame which had the similar shape (form) of the real automotive frame was fabricated. The contactless optical 3D scanner was used for the shape difference measurement of the frame between before and after the welding. The FE-model of the test frame was composed and the heat transfer and thermal stress simulation were performed. The simulated results were compared with the measured results for the reference of the frame design. The deformation shape of the frame by simulation was in good agreement with that by the experimental measurement.

• Journal of Embryo Transfer
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• v.11 no.1
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• pp.71-80
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• 1996

The envelope of the rnannnalian oocyte plays crucial roles in sperm-oocyte interactions by providing sperm receptors, inducing acrosome reaction and preventing polyspermy. Understanding of properties of the zona pellucida (ZP) is essential for the artificial control of fertility in mammals. This study was carried out to produce and characterize monoclonal antibodies(MAbs) to porcine ZP proteins. Approximately 8,000 ZPs were obtained from follicular oocytes and dissolved in 40$\mu$l of double distilled water. Following immunization through foot-pad injections of Balb /c mice with a ZP solution, the popliteal lymph nodes were recovered at 2 weeks after the last injection. Hybridoma cell lines were established by fusing lymph node cells with P3X63 myeloma cells through selection using HAT medium and screening by immunofluorescence(IF) microscopy on the isolated ZP. Secreted MAbs were found to consist k chains and different heavy chains as evidenced by isotyping. Some of the MAbs demonstrated high specificity to the ZP in IF. The Mabs also showed positive cross reactivity with hamster and mouse eggs, while negative with bovine eggs. The results implicate that the MAbs can be used not only for identification of functional regions of the ZP, but also for elucidation of mechanisms involved in fertilization of mammals. The MAbs will provide basic information on biochemical anatomy of the ZP as well as can be candidates for the future contraceptive vaccines.

• Journal of the Korean Applied Science and Technology
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• v.20 no.2
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• pp.148-153
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• 2003

Electron transfer through an Langmuir-Blodgett(LB) monolayer film sandwiched between metal electrodes. We used an eicosanoic acid material and the material was very famous as a thin film insulating material. Eicosanoic acid monolayer was deposited by Langmuir-Blodgett(LB) technique and a subphase was a $CdCl_2$ solution as a 2${\times}10^{-4}$ mol/L. Also we used a bottom electrode as an Al/$Al_2O_3$ and a top electrode as a Al and Ti/Al. Here, the $Al_2O_3$ on the bottom electrode was deposited by thermal evaporation method. The $Al_2O_3$ layer was acted on a tunneling barrier and insulating layer in tunnel diode. It was found that the proper transfer surface pressure for film deposition was 25 mN/m and the limiting area per molecule was about 24 ${\AA}^2$/molecule. When the positive and negative bias applied to the molecular device, the behavior shows that a tunnel switching characteristics. This result were analyzed regarding various mechanisms.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.1 no.3
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• pp.244-251
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• 1989

A numerical study has been performed on the natural convection heat transfer from a conducting tube with two axial fins to a surrounding cylinder. As increasing dimensionless fin length ($L_F$), the center of flow moves to the bottom of annulus and the recirculating flow rate is decreased. The maximum local Nusselt number of conducting tube appears at ${\theta}=180^{\circ}$ for $L_F=0.0$, but at ${\theta}=130^{\circ}$ for $L_F{\geq}0.3$ and that of outer cylinder appears at ${\theta}=13^{\circ}$ for $L_F{\leq}0.6$ but at ${\theta}=33^{\circ}$ for $L_F=1.0$. The fin temperature is decreased by increasing radial distance and the temperature distribution of the downward fin is generally less than that of the upward fin. By increasing fin length, the local Nusselt number of the upward fin appears negative values for $L_F=1.0$, but appears positive values for $L_F<0.8$, and that of the downward fin appears positive values.

• The Transactions of the Korean Institute of Electrical Engineers C
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• v.55 no.12
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• pp.586-590
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• 2006

Dendrimers represent a new class of synthetic macromoleculcs characterized by a regularly branched treelike structure. Multiple branching yields a large number of chain ends that distinguish dendrimers from conventional star-like polymers and microgels. The azobenzene dendrimer is one of the dendrimeric macromolecules that include the azo-group exhibiting a photochromic character. Due to the presence of the charge transfer element of the azo-group and its rod-shaped structure, these compounds are expected to have potential interest in electronics and photoelectronics, especially in nonlinear optics. In the present paper, we give pressure stimulation to organic thin films and detect the induced displacement current. Functional photoisometrization organic molecular the photo-stimulus to organic monomolecular L films and LB films of dendrimer and 8A5H were performed. The 8A5H organic monolayer in case of pressure stimulus occurred that positive course but in case of the photo-stimulus compared positive and negative. It is assumed that generation forms of displacement current were measured when photo-stimulus for impression.