• 제목/요약/키워드: negative indicator

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Identification and Partial Characterization of Cerein BS229, a Bacteriocin Produced by Bacillus cereus BS229

  • Paik, Hyun-Dong;Lee, Na-Kyoung;Lee, Kwang-Ho;Hwang, Yong-Il;Pan, Jae-Gu
    • Journal of Microbiology and Biotechnology
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    • v.10 no.2
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    • pp.195-200
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    • 2000
  • Bacillus cereus BS229 was identified as a bacteriocin producer with a bactericidal activity against Bacillus thuringiensis subsp. Thomsoni BR-40. Bacillus cereus BS229 and cerein BS229, named tentatively as the bacteriocin produced by Bacillus cereus BS229, showed a narrow spectrum of actibity against Gram-positive and Gram-negative bacteria, along with yeast and molds. Production of cerein BS229 in a 5-1 fermenter followed typical kinetics of primary metabolite synthesis. The antibacterial activity of cerein BS229 on sensitive indicator cells disappeared completely by ${\alpha}-chmotrypsin$ or proteinase K, which indicates its proteinaceous nature. Cerein BS229 seemed to be very stable throughout the pH range of 2.0 of 9.0 and it was relatively heat labile, despite the fact that bacteriocin activity was still detected after being boied for 30min. Cerein BS229 actibity has been changed with some of the organic solvents such as toluene, ethanol, and chloroform. Direct detection of cerein BS229 actibity on SDS-PAGE suggested that it had an apparent molecular mass of about 8.2 kDa.

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A Measure for Travel Time Reliability (통행시간 신뢰성 지표 개발 및 산정에 관한 연구)

  • Chang, Justin Su-Eun;Kang, Ji-Hye;Lee, Seung-Jun
    • Journal of Korean Society of Transportation
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    • v.26 no.5
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    • pp.217-226
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    • 2008
  • The term, travel-time reliability, refers to variations in journey time that travelers cannot predict. The purpose of this paper is to suggest a standard way to measure travel time reliability. A modified buffer time indicator is proposed. The index is represented by the difference between planned and actual travel times based on lognormal type travel time distribution. Using this framework, a constant function for railways and a negative parabola function for roads are discussed. The model developed is applied to the real data of Korean road and rail usages to empirically verify the methodology proposed. In this process, the unit value of travel time reliability for each group is estimated. The result of this research is expected to be helpful of conducting more cautious economic feasibility studies of transport.

Biological and Molecular Variability of Alfalfa mosaic virus Affecting Alfalfa Crop in Riyadh Region

  • AL-Saleh, Mohammed A.;Amer, Mahmoud A.
    • The Plant Pathology Journal
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    • v.29 no.4
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    • pp.410-417
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    • 2013
  • In 2011-2012, sixty nine samples were collected from alfalfa plants showing viral infection symptoms in Riyadh region. Mechanical inoculation with sap prepared from two collected samples out of twenty five possitive for Alfalfa mosaic virus (AMV) by ELISA were produced systemic mosaic on Vigna unguiculata and Nicotiana tabacum, local lesion on Chenopodium amaranticolor and C. quinoa. Vicia faba indicator plants that induce mosaic and mottle with AMV-Sagir isolate and no infection with AMV-Wadi aldawasser isolate. Approximately 700-bp was formed by RT-PCR using AMV coat protein specific primer. Samples from infected alfalfa gave positive results, while healthy plant gave negative result using dot blot hybridization assay. The nucleotide sequences of the Saudi isolates were compared with corresponding viral nucleotide sequences reported in GenBank. The obtained results showed that the AMV from Australia, Brazil, Puglia and China had the highest similarity with AMV-Sajer isolate. While, the AMV from Spain and New Zealaland had the lowest similarity with AMV-Sajer and Wadi aldawasser isolates. The data obtained in this study has been deposited in the GenBank under the accession numbers KC434083 and KC434084 for AMV-Sajer and AMV-Wadialdawasser respectively. This is the first report regarding the gnetic make up of AMV in Saudi Arabia.

Empirical Evidence on the Usefulness of Throughput Time (통과시간지표의 유용성에 관한 실증연구)

  • 육근효
    • Korean Management Science Review
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    • v.19 no.1
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    • pp.75-88
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    • 2002
  • In recent years, it Is necessary to develop an alternative measure, the time efficiency of management activities, as a more measurable and operational tool Instead of traditional accounting measures such as inventories turnover. Therefore the concept of throughput time has got much attention as useful tool for controlling time based management. The purpose of this paper is to investigate the usefulness and adaptability of throughput time. The sample consists of 212 non-banking firms listed on the Korean Stock Exchange. The test Period were 10 years(1989-1998). The regression analysis for this study was performed using the cross-sectional data for the sample, and it was also performed for each Industry. The results show that net Income to tonal assets and labor productivity (value added Per employee) variables in the model was signiflcantly associated with throughput time. On the other hand, the relationship between throughput time and logistics cost to sales do largely not have statistical significance. Especially, it is found that the relationship do not have significance or negative response in food & beverage industry and wholesale & retail industry. In summary, the results show that the measure of throughput time can be an effective managerial Indicator for time based competition and management.

Oxidative Stress and Antioxidant Status during Transition Period in Dairy Cows

  • Sharma, N.;Singh, N.K.;Singh, O.P.;Pandey, V.;Verma, P.K.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.4
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    • pp.479-484
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    • 2011
  • The study was conducted on 20 Holstein X Sahiwal cross bred dairy cows, with an average milk production of $2,752{\pm}113.79$ liters in $284{\pm}5.75$ days during a single lactation, that were divided in to two groups of 10 animals. We investigated the oxidative stress and antioxidant status during the transition period in dairy cows. In this study, plasma level of MDA was considered as an indicator of lipid peroxidation and SOD, catalase, GSH and GSHPx as antioxidants. The lipid peroxidation was significantly (p<0.001) higher in cows during early lactation as compared to the cows in advanced pregnancy. A significant positive correlation (r = +0.831, p<0.01) was determined between MDA and catalase in early lactating cows. In early lactating cows, blood glutathione was significantly lower than in advanced pregnant cows. However, early lactating cows showed non-significant negative correlation for all antioxidant enzymes with lipid peroxidation. In conclusion, dairy cows seemed to have more oxidative stress and low antioxidant defense during early lactation or just after parturition than advanced pregnant cows, and this appears to be the reason for their increased susceptibility to production diseases (e.g. mastitis, metritis, retention of fetal membranes etc.) and other health problems.

Radioisotopic and Radiological Evaluation in Patient with Stress Fracture (피로골절 환자에서 골주사 소견과 방사선 소견의 비교)

  • Ko, Kwang-Seop;Kim, Jai-Young;Kang, Sung-Koo;Kim, So-Yon;Lee, Gwon-Jun
    • The Korean Journal of Nuclear Medicine
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    • v.21 no.1
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    • pp.39-46
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    • 1987
  • The stress fracture is a disease caused by and abnormal stress to the normal bone with constant, repeated pull. Early detection of stress fracture plays an important role in treatment and prevention of its complication. Bone scintigraphy was performed to evaluate 18 patients with stress fracture of the lower extremities from May, 1985 to April, 1987, in the Department of Internal Medicine of National Police Hospital. The results were as follows: 1) Seventeen of the 18 cases showed positive bone scans at the initial study performed from 1 week to S months after the onset of symtom. 2) Ten of the 18 patients had findings of stress fracture at the initial X-ray film. Two out of 8 negative case revealed positive findings in the follow-up studies. 3) The bone scans in the 2 cases taken 5 months after the onset of symton; the one showed only slightly increased radiouptake, the other showed no abnormal findings. In conclusion, bone scanning is a more sensitive indicator of early stress fracture than radiologic study. The healing phase is characterized by a gradual decline in radioactivity at the fracture site in concordance with subsidence of symptom.

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Measurement of the Moderator Temperature Coefficient of Reactivity for Pressurized Water Reactors

  • Yu, Sung-Sik;Kim, Se-Chang;Na, Young-Whan;Kim, H. S.;J. Y. Doo;Kim, D. K.;S. W. Long
    • Nuclear Engineering and Technology
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    • v.29 no.6
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    • pp.488-499
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    • 1997
  • The measurements of the moderator temperature coefficient (MTC) are performed to demonstrate that the calculational model produces results that are consistent with the measurements. Since negative MTC is also a technical specification value that may limit the cycle length, it is important to measure it as accurately as possible. In this report, preferred choice of test method depending on the time in cycle, best power indication and temperature definition in MTC calculation were determined based on the MTC test results taken during initial startup testing and at 2/3 cycle burnup in the Yonggwang nuclear power plant. The results show that the ratio and rodded methods provided good agreement with the predictions during initial startup testing. However, near end-of-cycle the depletion method gives better results, and so is suggested to be used in the MTC measurements at 2/3 cycle burnup. The use of primary Delta T power as a power indicator in the MTC calculations is highly advisable since it responds with good consistent results very quickly to changes unlike secondary calorimetric power. For the appropriate temperature definitions used in the MTC calculations, it is considered that the arithmetic average temperature measured simply by inlet and outlet thermocouples is preferred. Although volumetric average temperature provides better results, the improvement is not sufficient to compensate for the simplicity of calculations by arithmetic average temperature.

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Effect of NaCl, Gum Arabic and Microbial Transglutaminase on the Gel and Emulsion Characteristics of Porcine Myofibrillar Proteins

  • Davaatseren, Munkhtugs;Hong, Geun-Pyo
    • Food Science of Animal Resources
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    • v.34 no.6
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    • pp.808-814
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    • 2014
  • This study investigated the effect of gum arabic (GA) combined with microbial transglutaminase (TG) on the functional properties of porcine myofibrillar protein (MP). As an indicator of functional property, heat-set gel and emulsion characteristics of MP treated with GA and/or TG were explored under varying NaCl concentrations (0.1-0.6 M). The GA improved thermal gelling ability of MP during thermal processing and after cooling, and concomitantly added TG assisted the formation of viscoelastic MP gel formation. Meanwhile, the addition of GA decreased cooking yield of MP gel at 0.6 M NaCl concentration, and the yield was further decreased by TG addition, mainly attributed by enhancement of protein-protein interactions. Emulsion characteristics indicated that GA had emulsifying ability and the addition of GA increased the emulsification activity index (EAI) of MP-stabilized emulsion. However, GA showed a negative effect on emulsion stability, particularly great drop in the emulsion stability index (ESI) was found in GA treatment at 0.6 M NaCl. Consequently, the results indicated that GA had a potential advantage to form a viscoelastic MP gel. For the practical aspect, the application of GA in meat processing had to be limited to the purposes of texture enhancer such as restructured products, but not low-salt products and emulsion-type meat products.

The Environmental Perceptions of Pre-service Secondary School Teachers about the Global Environment (지구 환경에 대한 예비 중등 교사의 환경 인식)

  • Cheong, Cheol
    • Journal of the Korean earth science society
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    • v.24 no.4
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    • pp.273-280
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    • 2003
  • The purpose of this study is to investigate the environmental perceptions of pre-service secondary school teachers about the global environment. The Subjects are 133 pre-service secondary school teachers. As an indicator of environmental perceptions, the modified New Environmental Paradigm (NEP) instrument was used. This modified NEP instrument consists of four dimensions: the negative results of growth and technology, relation of humankind and nature, quality of life, and limits of biosphere. The results of this study suggested that a majority of pre-service secondary teachers held ecological environmental perceptions. They also had a similar environmental perception to education major. However, there were significant differences in environmental perceptions and the variables of education experience on environment-related subjects. Based on the findings of this study, it is recommended that environment-related subjects. Based on the findings of this study, it is recommended that environmental education be a mandatory course in education programs.

A meshfree adaptive procedure for shells in the sheet metal forming applications

  • Guo, Yong;Wu, C.T.;Park, C.K.
    • Interaction and multiscale mechanics
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    • v.6 no.2
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    • pp.137-156
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    • 2013
  • In this paper, a meshfree shell adaptive procedure is developed for the applications in the sheet metal forming simulation. The meshfree shell formulation is based on the first-order shear deformable shell theory and utilizes the degenerated continuum and updated Lagrangian approach for the nonlinear analysis. For the sheet metal forming simulation, an h-type adaptivity based on the meshfree background cells is considered and a geometric error indicator is adopted. The enriched nodes in adaptivity are added to the centroids of the adaptive cells and their shape functions are computed using a first-order generalized meshfree (GMF) convex approximation. The GMF convex approximation provides a smooth and non-negative shape function that vanishes at the boundary, thus the enriched nodes have no influence outside the adapted cells and only the shape functions within the adaptive cells need to be re-computed. Based on this concept, a multi-level refinement procedure is developed which does not require the constraint equations to enforce the compatibility. With this approach the adaptive solution maintains the order of meshfree approximation with least computational cost. Two numerical examples are presented to demonstrate the performance of the proposed method in the adaptive shell analysis.