Browse > Article
http://dx.doi.org/10.5713/ajas.2011.10220

Oxidative Stress and Antioxidant Status during Transition Period in Dairy Cows  

Sharma, N. (Division of Veterinary Clinical Medicine & Jurisprudence, F.V.Sc, SKUAST-J)
Singh, N.K. (Division of Veterinary Clinical Medicine & Jurisprudence, F.V.Sc, SKUAST-J)
Singh, O.P. (Division of Veterinary Clinical Medicine & Jurisprudence, F.V.Sc, SKUAST-J)
Pandey, V. (Division of Veterinary Clinical Medicine & Jurisprudence, F.V.Sc, SKUAST-J)
Verma, P.K. (Division of Veterinary Clinical Medicine & Jurisprudence, F.V.Sc, SKUAST-J)
Publication Information
Asian-Australasian Journal of Animal Sciences / v.24, no.4, 2011 , pp. 479-484 More about this Journal
Abstract
The study was conducted on 20 Holstein X Sahiwal cross bred dairy cows, with an average milk production of $2,752{\pm}113.79$ liters in $284{\pm}5.75$ days during a single lactation, that were divided in to two groups of 10 animals. We investigated the oxidative stress and antioxidant status during the transition period in dairy cows. In this study, plasma level of MDA was considered as an indicator of lipid peroxidation and SOD, catalase, GSH and GSHPx as antioxidants. The lipid peroxidation was significantly (p<0.001) higher in cows during early lactation as compared to the cows in advanced pregnancy. A significant positive correlation (r = +0.831, p<0.01) was determined between MDA and catalase in early lactating cows. In early lactating cows, blood glutathione was significantly lower than in advanced pregnant cows. However, early lactating cows showed non-significant negative correlation for all antioxidant enzymes with lipid peroxidation. In conclusion, dairy cows seemed to have more oxidative stress and low antioxidant defense during early lactation or just after parturition than advanced pregnant cows, and this appears to be the reason for their increased susceptibility to production diseases (e.g. mastitis, metritis, retention of fetal membranes etc.) and other health problems.
Keywords
Antioxidative Enzymes; Oxidative Stress; Production Diseases; Transition Period; Dairy Cow;
Citations & Related Records

Times Cited By Web Of Science : 5  (Related Records In Web of Science)
Times Cited By SCOPUS : 0
연도 인용수 순위
  • Reference
1 Valko, M., D. Leibfritz, J. Moncol, M. T. Cronin, M. Mazur and J. Telser. 2007. Free radicals and antioxidants in normal physiological functions and human disease. Int. J. Biochem. Cell Biol. 39:44-84.   DOI   ScienceOn
2 Vannucchi, H., A. A. Jordoa-Junior, A. C. Iglessias, M. V. Morandi and P. G. Chiarello. 1997. Effects of different diatery concentrations of vitamin E on lipid peroxidation in rats. Arch. Latinoam. Nutr. 47:34-37.
3 Waller, K. P. 2000. Mammary gland immunology around parturition. Influence of stress, nutrition and genetics. Adv. Exp. Med. Biol. 480: 231-245.
4 Weiss, W. P., J. S. Hogan, D. A. Todhunter and K. L. Smith. 1997. Effect of vitamin E supplementation in diets with a low concentration of selenium on mammary gland health of dairy cows. J. Dairy Sci. 80:1728-1737.   DOI   ScienceOn
5 Ohkawa, H., N. Ohishi and K. Yagi. 1979. Assay for lipid peroxides in animal tissues by Thiobarbituric acid reaction. Anal. Chem. 95:351-358.
6 Ronchi, B., U. Bernabucci, N. Lacetera and A. Nardone. 2000. Oxidative and metabolic status of high yielding dairy cows in different nutritional conditions during the transition period. In: Proceedings of 51st Annual Mtg. E.A.A.P., Vienna. p. 125.
7 Saleh, M., A. Salam and I. M. H. MEL Mileegy. 2007. Oxidative antioxidant status during transition from late pregnancy to early lactation in native and cross bred cows in the Egyptian oasis. Assiut. Vet. Med. J. 53:113.
8 Shanks, R. D., A. E. Freeman and F. N. Dickinson. 1981. Postpartum distribution of costs and disorders of health. J. Dairy Sci. 64:683.   DOI
9 Snedecor, G. W. and W. G. Cochran. 1994. Statistical methods. 8th Ed. Iowa State University Press, Ames, Iowa, USA.
10 Sordillo, L. M. and S. L. Aitken. 2009. Impact of oxidative stress on the health and immune function of dairy cattle. Vet. Immunol. Immunopathol. 128:104-109.   DOI   ScienceOn
11 Sordillo, L. M., G. A. Contreras and S. L. Aitken. 2009. Metabolic factors affecting the inflammatory response of periparturient dairy cows. Anim. Health Res. Rev. 10:53-63.   DOI   ScienceOn
12 Sordillo, L. M., N. O'Boyle, J. C. Gandy, C. M. Corl and E. Hamilton. 2007. Shifts in thioredoxin reductase activity and oxidant status in mononuclear cells obtained from transition dairy cattle. J. Dairy Sci. 90:1186-1192.   DOI   ScienceOn
13 Sordillo, L. M. 2005. Factors affecting mammary gland immunity and mastitis susceptibility. Livest. Prod. Sci. 98:89-99.   DOI   ScienceOn
14 Halliwell, B. and M. Whiteman. 2004. Measuring reactive species and oxidative damage in vivo and in cell culture: how should you do it and what do the results mean?. Br. J. Pharmacol. 142:231-255.   DOI   ScienceOn
15 Trevisan, M., R. Browne, M. Ram, P. Muti, J. Freudenheim, A. N. Carosella and D. Armstrong. 2001. Correlates of markers of oxidative status in the general population. Am. J. Epidemiol. 154:348-356.   DOI   ScienceOn
16 Hafeman, D. G., R. A. Sunde and W. G. Hoekstra. 1974. Effect of dietary selenium on erythrocyte and liver glutathione peroxidase in the rats. J. Nutr. 104:580-587.
17 Halliwell, B. and J. M. C. Gutteridege. 2007. Free radicals in biology and medicine, 4th Ed. Oxford University Press. Grune Strottan, New York.
18 Holbrook, J. and C. L. Hicks. 1978. Variation of superoxide dismutase in bovine milk. J. Dairy Sci. 61:1072.   DOI
19 Kale, M., N. Rathore, S. John and D. Bhatnagar. 1999. Lipid peroxidative damage on pyrethroid exposure and alterations in antioxidant status in rat erythrocytes: a possible involvement of reactive oxygen species. Toxicol. Lett. 105:197-205.   DOI   ScienceOn
20 Kumaraguruparan, R., R. Subapriya, J. Kabalimoorthy and S. Nagini. 2002. Antioxidant profile in the circulation of patients with fibroadenoma and adenocarcinoma of the breast. Clin. Biochem. 35:275-279.   DOI   ScienceOn
21 Machlin, L. J. and A. Bendich. 1987. Free radical tissue damage: protective role of antioxidant nutrients. Fed. Am. Soc. Exp. Biol. 1:441.
22 Madsen, F. C., R. E. Rompala and J. K. Miller. 1990. Effect of disease on the metabolism of essential trace elements: a role for dietary coordination complexes. Feed Manage. 41:20.
23 Aitken, S. L., E. L. Karcher, P. Rezamand, J. C. Gandy, M. J. Vandehaar, A. V. Capuco and L.M. Sordillo. 2009. Evaluation of antioxidant and proinflammatory gene expression in bovine mammary tissue during the transition period. J. Dairy Sci. 92:589-598.   DOI   ScienceOn
24 Madsen, F. C. 1991. Disease and stress: a reason to consider the use of organically complexed trace elements. In: Biotechnology in the Feed Industry. Proceedig of the Alltech 7th Annual Symposium. Alltech Tech. Publ., Nicholasville, KY. p. 147.
25 Marklund, S. and G. Marklund. 1974. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur. J. Biochem. 47:469-476.   DOI   ScienceOn
26 Aebi, H. 1983. Catalase. In Methods Enzymology (Ed. H. U. Berg Meyer). pp. 276-286. Academic Press, New York.
27 Allison, R. D. and R. A. Laven. 2000. Effect of vitamin E supplementation on the health and fertility of dairy cows: a review. Vet. Rec. 147:703-708.
28 Balasinska, B. 2004. Evaluation of antioxidant status in living organisms. Med. Weter. 60:579-583.
29 Bernabucci, U., B. Ronchi, N. Lacetera and A. Nardone. 2005. Influence of body condition score on relationships between metabolic status and oxidative stress in periparturient dairy cows. J. Dairy Sci. 88:2017-2026.   DOI   ScienceOn
30 Beutler, E. 1963. Red cell metabolism. In a manual of biochemical methods, pp. 67-69.
31 Goff, J. P. and R. L. Horst. 1997. Physiological changes at parturition and their relationship to metabolic disorders. J. Dairy Sci. 80:1260-1268.   DOI   ScienceOn
32 Formigoni, A., D. Calderone, P. Pezzi and A. Panciroli. 1997. Evolution of oxidative status in dairy cows: preliminary observations. In: Proceedings of the 12th Associazione Scientifica Produzioni Animali Congress, Pisa. pp. 203-204.
33 Gitto, E., R. J. Reiter, M. Karbownik, D. X. Tan, P. Gitto, S. Barberi and I. Barberi. 2002. Causes of oxidative stress in the pre- and perinatal period. Biol. Neonate 81:146-157.   DOI   ScienceOn