• Journal of Korean Society of Forest Science
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• v.82 no.4
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• pp.328-336
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• 1993

This study was conducted to investigate sensitivity of tree seedlings to $SO_2$. Visible injury symptoms and changes of ethylene production were investigated in tree seedlings with the fumigation of $SO_2$ in gas chamber 4 hours a day for six days. The symptoms of visible injury did not appear below 0.5ppm level of $SO_2$ exposure but a change of visible injury with the passage of time appeared at 1.5 and 2.5ppm in all seedlings. With the higher the concentration and/or the longer exposure of $SO_2$ the visible injury symptoms on leaves increased in all seedlings. The sensitivity of seedlings to $SO_2$ was the highest in Liriodendron tulipifera followed by Pinus strobus, Ginkgo biloba, Pinus densiflora and Pinus koraiensis. The amount of ethylene production was more at 1.5 and 2.5ppm of $SO_2$ exposure than at 0.5ppm and the peak time of it came faster at higher levels. The amount of ethylene production was significantly different among tree seedlings. It showed a higher at production of ethylene in Liriodendron tulipifera compared to Ginkgo biloba and the ethylene production of Pinus trees to $SO_2$ were the highest in Pinus strobus followed by Pinus densiflora and Pinus koraiensis. In needle of Pinus strobus the ethylene production increased with the increasing rate of visible injury until the injury rate of 40-50% and than decreased with the increasing rate of visible injury since the rate of 50%.

• Journal of Korea Foresty Energy
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• v.21 no.1
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• pp.7-15
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• 2002

The essential oil free needles, which were left after distillation of essential oil from various coniferous needles, were fermented with food waste organics in order to use as compost and roughage. Microorganisms for the fermentation were selected from domestic sources such as swine compost, bark compost, and kimchi, etc, and consisted of aerothermophilic bacteria and actinomycetes. The weight reduction ratio of food waste organics treated with the microorganisms was 90% after 30 days treatment, and the fermentation temperature was kept at approximately $45^{\circ}C$. The compost process was really slow due to chemical compounds derived from needles, and it finally took 60 days for complete compost. When 10% of needle compost was mixed with soil for radish growth, the growth indicators such as leaf length and root weight were increased compared with control, while root weight, root width, and root length were inhibited on the addition of 20% needle compost. The nutrient value and digestibility ratio of various essential oil free needles as roughage for ruminant animals were evaluated. The ratio of crude protein for essential oil free needles from Korean pine(Pinus koraiensis) was 10.02%, which was higher than those of rice straw(5.48%) and corn(9.00%). The digestibility ratios of essential oil free needles from Sawara cypress(Chamaecwaris pislfera), Korean pine(Pinus koraiensis), and Japanese Red Pine(Pinus densiflora) was 53%, 34%, 34%, respectively, indicating that those essential oil free needles were considered as excellent roughage.

• Korean journal of applied entomology
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• v.36 no.2
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• pp.150-155
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• 1997

Distribution of pine needle gall midge infestations was analyzed from data collected in young Japanese red pine stands during 1992, and 1995-1996 in Kangwon-do. No significant differences in percentages of infested needle pairs were found among trees and between terminal and lateral shoots within a tree. However, the mean percentages of infested needle pairs increased significantly from the lower crown to the upper. Percentages of infested needle pairs on sample units, consisting 1 terminal and 2 lateral shoots, in the midcrown were best predictors of whole-tree percentages than were other crown levels. Therefore, a sample unit consisting of 1 terminal shoot and 2 lateral shoots per branch were fixed from the midcrown level. Number of tree and sample unit combinations needed to estimate pine needle gall midge infestations with given two levels of precision were determined.

• Journal of Korea Foresty Energy
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• v.17 no.1
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• pp.8-22
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• 1998

The phytomass and productivity of the evergreen needle pines(Pinus densiflora and Pinus rigida) and of the deciduous broad- leaved oaks(Quercus acutissima, Quercus variabililis and Quercus mongolica) in the watershed forests of South Han River, central part of Korea was estimated by the allometry. The allometic relations between D$^2$H and dry weight of stem(Ws), branches(Wb) and leaves(Wl) of these forests were as follows: Pinus densiflora community : logWs=0.58482D$^2$H-0.42417 logWb=0.6234D$^2$H-1.2321 logWl=0.2247D$^2$H-0.09359 Pinus rigida community : logWs=0.9218D$^2$H-1.4692 logWb=1.0064D$^2$H-2.2278 logWl=0.6275D$^2$H-1.1715 Quercus acutissima community : logWs=0.96659D$^2$H-1.56975 logWb=0.86315D$^2$H-2.17944 logWl=0.79876D$^2$H-1.97137 Quercus variabilis community : logWs=0.4753D$^2$H＋0.20026 logWb=0.5725D$^2$H-0.92006 logW1=0.61649D$^2$H-1.4288 Quercus momgolica community : logWs=0.5526D$^2$H-0.1228 logWb=0.5188D$^2$H-0.9787 logWl=0.7754D$^2$H-2.6273 From the above formulae, the phytomass of Pinus densiflora was estimated to be much as 1,200,000 ton, Pinus rigida 610,000 ton, Quercus acutissima 289 ton, Quercus variabilis 2,800ton and Quercus mongolica 570,000 ton in the surveyed area, respectively. Accordingly, the phytomass of Pinus densiflora forest was occupied about 43.9 % in toto and was higher thanthose of other forests. The phytomass of watershed forests belonging to Degree 8 and Degree7 divided by degree of green naturality(DGN) was 74,000 ton and 2,000,000 ton, resfectively. Accordingly, the watershed forests in South Han River were mainly composed of Pinus densiflora and Quercus mongolica forests belonging to Degree 7.

• Journal of Forest and Environmental Science
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• v.15 no.1
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• pp.107-116
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• 1999

For the study on the variation of Pinus densiflora S. et Z. natural populations. 13 stands were selected in Kangwondo region in 1994. They were located in Hyoja-Dong, Chunchon-Si [plot 1]; Sinnam-Myun, lnje-Gun [plot 2]; Buk-Myun, lnje-Gun [plot 3]; Seo-Myun, Yangyang-Gun [plot 4 and 5]; Sonyang-Myun, Yangyang-Gun [plot 6]; Toseong-Myun, Goseong-Gun [plot 7]; Synrim-Myun, Wonju-Si [plot 8]; Dunnae-Myun, Hoengsong-Gun [plot 9]; Jinbu-Myun, Pyungchang-Gun [plot 10]; Sogumgang, Kangneung-Si [plot 11]; Seo-Myun, Ulchin-Gun [plot 12]; and Mitan- Myun, Pyungchang-Gun [plot 13]. The morphological characteristics of needles and cones were investigated for twenty individual trees from each population. The results are summarized as follows: 1. Among needle characteristics, length, the number of serration. and the number of stoma row were significantly different among populations and between individuals within a population. They were 69.3~91.9mm, 25.1~28.7 per 0.5cm, and 4.1~6.2 rows for abaxial side and 2.9~4.6 rows for adaxial side, respectively. 2. Among cone characters surveyed. length and diameter were significantly different among populations and between individuals within a population. They were 31.1~43.7mm and 20.0~24.1mm, respectively. 3. A highly positive correlation was observed between the number of stoma row of abaxial side and that of adaxial side. and between length and diameter of cone. But the number of serration was negatively correlated with all traits. 4. The distance from seashore was positively correlated with needle length and the number of serration, but negatively correlated with the number of stoma row and cone length. However, the altitude was positively correlated with the number of serration, but negatively correlated with the number of stoma row and cone length. 5. Cluster analysis using average linkage method for needle and cone characteristics showed two groups to Euclidean distance 1.0. They were group I consisting of plots 1, 8, 12, and 13 and group II of plots 2, 3, 4, 5, 6, 7, 9, 10, and 11. However, group II was divided again to Euclidean distance 0.8, that is a group including plots 2, 3, 9, and 11 and the other group comprising plots 4, 5, 6, 7, and 10.

• Journal of Life Science
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• v.27 no.6
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• pp.646-651
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• 2017

This study was carried out to examine the antimicrobial activity of 70% ethanol and hot water extracts from different parts and essential oil in Pinus densiflora on skin pathogens such as Staphylococcus epiermidis, S. aureus, Propionibacterium acnes, Candida albicans, C. tropicalis, and Trichophyton rubrum. The antimicrobial activities of extracts and essential oil were tested by paper disc assay and minimum inhibitory concentration test. The ethanol extract of pine pollen showed antimicrobial activity against C. tropicalis and T. rubrum. Ethanol extract of pine needle and pine gnarl showed antimicrobial activity against S. aureus, C. tropicalis, and P. acnes. Essential oil from pine needle exhibited antimicrobial activity against C. albicans, C. tropicalis, and P. acnes. The minimum inhibitory concentration of ethanol extracts of pine needle and pine gnarl against P. acnes and C. tropicalis ranged from 0.002% to 0.0063%. The lowest minimum inhibitory concentration (0.025%) against C. albicans and C. tropicalis was obtained from essential oil. These results indicate that ethanol extracts of pine pollen, pine needle, pine gnarl, and essential oil of pine needle could be applicable to control the skin infection pathogens. Especially, ethanol extract of pine gnarl had a broad spectrum of antimicrobial activity and pine extracts and essential oil exhibited higher antimicrobial activity with Candida sp. and P acnes.

• Microbiology and Biotechnology Letters
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• v.44 no.2
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• pp.208-217
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• 2016

Pinus densiflora Sieb. et Zucc. (P. densiflora) contains several phenolic compounds that exhibit biological activities, such as antimicrobial, antioxidant, and antihypertensive effects. However, the anti-inflammatory effect of P. densiflora on skin has rarely been reported. Malassezia furfur (M. furfur) is a commensal microbe that induces skin inflammation and is associated with several chronic disorders, such as dandruff, seborrheic dermatitis, papillomatosis, and sepsis. The aim of our study was to identify the anti-inflammatory effects of P. densiflora needle extracts on skin health subjected to M. furfur-induced inflammation. The methanolic extract of the pine needles was partitioned into n-hexane, EtOAc, n-BuOH, and water layers. We measured the anti-inflammatory effects (in macrophages) as well as the antioxidant, antifungal, and tyrosinase inhibitory activity of each of these layers. The antioxidant activity of the individual layers was in the order EtOAc layer > n-BuOH layer > water layer. Only the n-BuOH, EtOAc, and n-hexane layers showed antifungal activity. Additionally, all the layers possessed tyrosinase inhibition activity similar to that of ascorbic acid, which is used as a commercial control. The EtOAc layer was not cytotoxic toward the RAW 264.7 cell line. Interleukin 1 beta and tumor necrosis factor (TNF)-α expression levels in M. furfur-stimulated RAW 264.7 cells treated with the EtOAc layer were decreased markedly compared to those in cells treated with the other layers. Taken together, we believe that the needle extracts of P. densiflora have potential application as alternative anti-inflammatory agents or cosmetic material for skin health improvement.

• Nutrition Research and Practice
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• v.5 no.4
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• pp.281-287
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• 2011

In this study, we evaluated the antioxidant activity of pine needle extracts prepared with hot water, ethanol, hexane, hot water-hexane (HWH), and hot water-ethanol (HWE), using the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical method. The hot water extract possessed superior antioxidant activity than the other extracts. We also compared the antioxidant activity of pine needle extracts through ROS inhibition activity in a cellular system using MC3T3 E-1 cells. The hot water extract exhibited the lowest ROS production. The pattern of HPLC analysis of each extract indicated that the hot water extract contained the highest proanthocyanidin level. The pine needle hot-water extract was then isolated and fractionated with Sephadex LH-20 column chromatography to determine the major contributor to its antioxidant activity. The No.7 and 12 fractions had high antioxidant activities, that is, the highest contents of proanthocyanidins and catechins, respectively. These results indicate that the antioxidant activity of procyanidins from the hot water extract of pine needles is positively related to not only polymeric proanthocyanidins but also to monomeric catechins. Moreover, the antioxidant activity of the pine needle hot water extract was similar to well-known antioxidants, such as vitamin C. This suggests that pine needle proanthocyanidins and catechins might be of interest for use as alternative antioxidants.

• Journal of Korean Society of Forest Science
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• v.61 no.1
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• pp.20-26
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• 1983

Some aspects of the change in the rate of gall formation on the red pine, Pinus densiflora Siebold et Zuccarini, by the pine needle gall midge, Thecodiplosis japonensis Uchida et Inouye, were investigated at the coastal area in Chung Nam-Do from 1977 to 1982, and were analyzed with the nationwide survey data for 2 years from 1980 to 1981. The results obtained were as follows; the gall formation rate in several areas was compared between two consecutive generations. Four regression equations were established, with X as the gall formation rate in N year and Y as that in (N+1) year: 1) Y=11.2+1.15X (r=0.833) for the stands facing north on the phase of insect population increase, 2) Y=14.3+0.82X (r=0.739) for the stands facing south on the phase of population increase, 3) Y=7.3+0.46X (r=0.478) for stands facing north on the phase of population decrease and 4) Y=11.4+0.19X (r=0.086) for stands facing south on the phase of population decrease. After invasion of the pine needle gall midge into a red pine forest, gall formation rate increased for 6 consecutive generations and then decreased to low level within a period of another 6 years.

• Korean Journal of Food Science and Technology
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• v.30 no.2
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• pp.450-455
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• 1998

Pine has been known as a traditional medicinal plant and as showing a physically beneficial function to a human being. Therefore, this study was performed to investigate the physiological activities of main pine neddles. Ethanol extracts from pinus needles did net exhibit any mutagenicity. On the contrary, inhibitory effects of ethanol extract were observed on mutagenicity induced by N-methyl-N'-nitro-N-nitrosoguanidine(MNNG), 4-nitroquinoline-1-oxide (4NQO), 3-amino-1,4-dimethyl-5H-pyrido-(4,3-b)indol (Trp-P-1) and benzo(a)pyrene $(B({\alpha})P)$ using Salmonella typhimurium reversion assay. On direct-acting mutagen (MNNG, 4NQO) and indirect-acting mutagen (Trp-P-1, $(B({\alpha})P)$, we observed higher inhibitory effect. Stepwise fractionation of the ethanol extract was done by using ether, chloroform, ethyl acetate, butanol and water to obtain effective fraction. Among them, water fractions $(100\;{\mu}g/plate)$ of Pinus thunbergii, Pinus rigida, Pinus densiflora and Pinus koraiensis showed high inhibition of 91.65%, 94.7%, 84.22% and 79.02%, respectively, on the mutagenicity of MNNG in Salmonella typhimurium TA100.