Antimicrobial Activity of Extracts from Different Parts and Essential Oil from Pinus densiflora on Skin Pathogens

소나무 부위별 추출물 및 essential oil의 피부상재균에 대한 항균 활성

  • Park, Sun-Hee (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Kim, Koth-Bong-Woo-Ri (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Kim, Min-Ji (Department of Food Science & Technology/Institute of Food Science, Pukyong National University) ;
  • Choi, Jung-Su (Subdivision of Culinary Arts, Kyungnam College of Information and Technology) ;
  • Cho, Young-Je (School of Food Science of Biotechnology, Kyungpook National University) ;
  • Ahn, Dong-Hyun (Department of Food Science & Technology/Institute of Food Science, Pukyong National University)
  • 박선희 (부경대학교 식품공학과/식품연구소) ;
  • 김꽃봉우리 (부경대학교 식품공학과/식품연구소) ;
  • 김민지 (부경대학교 식품공학과/식품연구소) ;
  • 최정수 (경남정보대학교 호텔외식조리계열) ;
  • 조영제 (경북대학교 식품공학부) ;
  • 안동현 (부경대학교 식품공학과/식품연구소)
  • Received : 2016.12.30
  • Accepted : 2017.06.19
  • Published : 2017.06.30


This study was carried out to examine the antimicrobial activity of 70% ethanol and hot water extracts from different parts and essential oil in Pinus densiflora on skin pathogens such as Staphylococcus epiermidis, S. aureus, Propionibacterium acnes, Candida albicans, C. tropicalis, and Trichophyton rubrum. The antimicrobial activities of extracts and essential oil were tested by paper disc assay and minimum inhibitory concentration test. The ethanol extract of pine pollen showed antimicrobial activity against C. tropicalis and T. rubrum. Ethanol extract of pine needle and pine gnarl showed antimicrobial activity against S. aureus, C. tropicalis, and P. acnes. Essential oil from pine needle exhibited antimicrobial activity against C. albicans, C. tropicalis, and P. acnes. The minimum inhibitory concentration of ethanol extracts of pine needle and pine gnarl against P. acnes and C. tropicalis ranged from 0.002% to 0.0063%. The lowest minimum inhibitory concentration (0.025%) against C. albicans and C. tropicalis was obtained from essential oil. These results indicate that ethanol extracts of pine pollen, pine needle, pine gnarl, and essential oil of pine needle could be applicable to control the skin infection pathogens. Especially, ethanol extract of pine gnarl had a broad spectrum of antimicrobial activity and pine extracts and essential oil exhibited higher antimicrobial activity with Candida sp. and P acnes.

본 연구는 소나무 부위별 추출물을 피부염 유발 균주에 적용함으로써 항균 및 항진균 활성을 측정하고 그 결과를 보고하고자 하였다. 먼저 paper disc 법을 이용하여 항균 및 항진균 실험에서 송엽, 송절 및 송화 에탄올 추출물은 대부분의 피부염 균주에 대하여 항균활성을 나타내었으나, 물 추출물은 모든 피부염 균주에 대하여 항균활성을 나타내지 않았다. 송엽 및 송절 70% 에탄올 추출물이 T. rubrum을 제외한 모든 피부염 균주에 대하여 항균활성을 나타내었으며, 송화 70% 에탄올 추출물은 C. tropicalis와 P. acens에 대해서만 항균활성을 보였다. 소나무 essential oil의 항균 및 항진균 활성을 측정한 결과, C. albicans, C. tropicalis, T. rubrum에 대해 1.5~3 mm 정도의 생육저해환을 형성하여 높은 항진균 활성을 나타내었다. 이러한 결과를 바탕으로 소나무 부위별 70% 에탄올 추출물에 활성을 나타내었던 피부염 관련 균주 6종에 대한 최소생육 저해 농도를 측정한 결과, 송엽 70% 에탄올 추출물은 C. tropicalis 및 P. acnes에 대하여 0.002%와 0.0063%의 낮은 농도에서 항균효과가 나타났고 송절 70% 에탄올 추출물은 0.003% 농도에서 P. acnes의 생육을 억제하여 뛰어난 효과를 보였다. 소나무 essential oil의 최소생육 저해 농도를 측정한 결과, 0.025% 농도에서 C. albicans와 C. tropicalis의 생육을 효과적으로 억제하는 것으로 나타났다. 송엽 essential oil은 C. albicans, C. tropicalis, T. rubrum에 대해 항진균 효과가 있는 것으로 확인되었다. 이상의 결과를 통해 소나무 부위 중 송엽과 송절 70% 에탄올 추출물에서 항균 및 항진균 효과가 뛰어났으며, 특히, 송절 70% 에탄올 추출물이 S. aureus, S. epidermidis, P. acnes에 대해 더 높은 항균 효과를 나타내었다. 따라서, 소나무 송절과 송엽 에탄올 추출물과 essential oil은 Candida 속과 P. acnes에 대한 억제효과가 크게 나타나 이와 관련한 피부질환 개선에 효과가 클 것으로 사료된다.



  1. Bensouilah, J. and Buck, P. 2006. Aromadermatology: Aromatherapy in the treatment and care of common skin conditions, pp. 1-8, ed., Radcliffe Publishing, Oxon, United Kingdom.
  2. Choi, S. W. and Ahn, S. K. 2016. A statistical study of dermatoses in kangwon province, 2010-2014. Kor. J. Dermatol. 54, 26-33.
  3. Han, J. S. and Yi, D. H. 2012. Effects of pine needles fermentationextracts on antioxidant activity and inhibition of melanin synthe-sis. Kor. J. Aesthet. Cosmetol. 10, 619-624.
  4. Hong, T. G. 2003. Studies on the physiological functionality and antimicrobial activity of Pine needle fermentation extract. Master Dissertation, Daegu University, Gyeongsan-si, Korea.
  5. Jang, S. J. and Ahn, K. J. 2004. Superficial dermatomycosis and the causative agents in Korea. Kor. J. Med. Mycol. 9, 91-99.
  6. Kazemi, M., Rostami, H. and Shafiei, S. 2012. Antibacterial and antifungal activity of some medicinal plants from Iran. J. Plant Sci. 7, 55-66.
  7. Kim, C. 2012. A comparison study of antibacterial effects of pine needle extracts on human skin pathogens. Kor. J. Aesthet. Cosmetol. 10, 25-30.
  8. Kim, M. R., Woo, S. E., Shin, S. O., Hong, S. M. and Yang, S. Y. 2006. A study on the distribution of staphylococcus aureus in atopic dermatitis. J. Soc. Cosmet. Scientists Korea 32, 93-97.
  9. Kim, Y. J., Cho, B. J., Ko, M. S., Jung, J. M., Kim, H. R., Song, H. S., Lee, J. Y., Sim, S. S. and Kim, C. J. 2010. Anti-oxidant and anti-aging activities of essential oils of Pinus densiflora needles and twigs. Yakhak Hoeji 54, 215-225.
  10. Koukos, P. K., Papadopoulou, K. I., Patiaka, D. T. and Papagiannopoulos, A. D. 2000. Chemical composition of essential oils from needles and twigs of balkan pine (Pinus peuce Grisebach) grown in Northern Greece. J. Agric. Food Chem. 48, 1266-1268.
  11. Lambert, R. J. W., Skandamis, P. N., Coote, P. J. and Nychas, G. H. E. 2011. A study of the minimum inhibitory concentration and mode of action of oregano essential oil, thymol and carvacrol. J. Appl. Microbiol. 91, 453-562.
  12. Lee, D. K., Moon, C. M. and Koh, J. K. 2006. Clinical and mycological studies on superficial fungal infection. Kor. J. Med. Mycol. 11, 54-63.
  13. Lee, S. Y., Kim, J. H., Kim, K. B. W. R., Song, E. J., Kim, A. R., Park, S. M., Han, C. S. and Ahn, D. H. 2007. Antimicrobial activities of medicinal herbs and seaweeds extracts against microorganisms isolated from the rice warehouses. J. Kor. Soc. Food Sci. Nutr. 36, 476-480.
  14. Lim, Y. S., Bae, M. J. and Lee, S. H. 2002. Antimicrobial effects of Pinus densiflora Sieb. et Zucc. ethanol extract on Listeria monocytogenes. J. Kor. Soc. Food Sci. Nutr. 31, 333-337.
  15. Park, C. S. 1998. Antibacterial activity of ethanol extract of pine needle against pathogenic bacteria. Kor. J. Postharvest Sci. Technol. 5, 380-385.
  16. Pfaller, M. A., Jones, R. N., Messer, S. A., Edmond, M. B. and Wenzel, R. P. 1998. National surveillance of nosocomial bloodstream infection due to Candida albicans : frequency of occurrence and antifungal susceptibility in the SCOPE program. Diagn. Microbiol. Infect. Dis. 31, 327-332.
  17. Ramier, S. S. 2000. Managing pediatric atopic dermatitis. Clin. Pediatr. 39, 1-14.
  18. Sung, H. C., Jung, H. D., Park, K. D., Lee, W. J., Lee, S. J. and Kim, D. W. 2007. A quantitative culture study of Staphylococcus aureus in adolescent and adult patients with atopic dermatitis using the contact-plate sampling technique. Kor. J. Dermatol. 45, 673-679.
  19. Winston, M. H. and Shalita, A. R. 1991. Acne vulgaris, pathogenesis and treatment. Pediatr. Clin. N. Am. 38, 889-903.
  20. Yoon, S. Y., Lee, S. Y., Kim, K. B. W. R., Song, E. J., Kim, S. J., Lee, S. J., Lee, C. J. and Ahn, D. H. 2009. Antimicrobial activity of the solvent extract from different parts of Orostachys japonicus. J. Kor. Soc. Food Sci. Nutr. 38, 14-18.
  21. Yousif, N. I. M. and Dabbagh, R. A. 2016. Isolation and identification of microorganisms in acne patients. Zanco J. Med. Sci. 20, 1330-1336.
  22. Zeng, W. C., Zhang, Z., Gao, H., Jia, L. R. and He, Q. 2012. Chemicalcomposition, antioxidant, and antimicrobial activities of essential oil from pine needle (Cedrus deodara). J. Food Sci. 77, C824-C829.