Browse > Article
http://dx.doi.org/10.4014/mbl.1603.03006

The Beneficial Effects of Extract of Pinus densiflora Needles on Skin Health  

Choi, Jieun (Department of Life Science & BK21-Plus Research Team for Bioactive Control Technology, Chosun University)
Kim, Woong (Department of Life Science & BK21-Plus Research Team for Bioactive Control Technology, Chosun University)
Park, Jaeyoung (Department of Life Science & BK21-Plus Research Team for Bioactive Control Technology, Chosun University)
Cheong, Hyeonsook (Department of Life Science & BK21-Plus Research Team for Bioactive Control Technology, Chosun University)
Publication Information
Microbiology and Biotechnology Letters / v.44, no.2, 2016 , pp. 208-217 More about this Journal
Abstract
Pinus densiflora Sieb. et Zucc. (P. densiflora) contains several phenolic compounds that exhibit biological activities, such as antimicrobial, antioxidant, and antihypertensive effects. However, the anti-inflammatory effect of P. densiflora on skin has rarely been reported. Malassezia furfur (M. furfur) is a commensal microbe that induces skin inflammation and is associated with several chronic disorders, such as dandruff, seborrheic dermatitis, papillomatosis, and sepsis. The aim of our study was to identify the anti-inflammatory effects of P. densiflora needle extracts on skin health subjected to M. furfur-induced inflammation. The methanolic extract of the pine needles was partitioned into n-hexane, EtOAc, n-BuOH, and water layers. We measured the anti-inflammatory effects (in macrophages) as well as the antioxidant, antifungal, and tyrosinase inhibitory activity of each of these layers. The antioxidant activity of the individual layers was in the order EtOAc layer > n-BuOH layer > water layer. Only the n-BuOH, EtOAc, and n-hexane layers showed antifungal activity. Additionally, all the layers possessed tyrosinase inhibition activity similar to that of ascorbic acid, which is used as a commercial control. The EtOAc layer was not cytotoxic toward the RAW 264.7 cell line. Interleukin 1 beta and tumor necrosis factor (TNF)-α expression levels in M. furfur-stimulated RAW 264.7 cells treated with the EtOAc layer were decreased markedly compared to those in cells treated with the other layers. Taken together, we believe that the needle extracts of P. densiflora have potential application as alternative anti-inflammatory agents or cosmetic material for skin health improvement.
Keywords
Pinus densiflora; Malassezia furfur; skin health; anti-inflammation;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Nenadis N, Tsimidou M. 2002. Observations on the estimation of scavenging activity of phenolic compounds using rapid 1,1-diphenyl-2-picrylhydrazyl (DPPH•) tests. J. Am. Oil Chem. Soc. 79: 1191−1195.   DOI
2 Park H-H, Lee S, Oh J-M, Lee M-S, Yoon K-H, Park BH, et al. 2007. Anti-inflammatory activity of fisetin in human mast cells (HMC-1). Pharmacol. Res. 55: 31−37.   DOI
3 Park J, Choi H, Kim W, Kim H, Cheong H. 2015. Antithrombosis activity of protocatechuic and shikimic acids from functional plant Pinus densiflora Sieb. et Zucc needles. J. Nat. Med. DOI:10.1007/s11418-015-0956-y
4 Park YS, Jeon MH, Hwang HJ, Park MR, Lee S-H, Kim SG, et al. 2011. Antioxidant activity and analysis of proanthocyanidins from pine (Pinus densiflora) needles. Nutr. Res. Prac. 5: 281−287.   DOI
5 Ryu S, Choi S-Y, Acharya S, Chun Y-J, Gurley C, Park Y, et al. 2011. Antimicrobial and anti-inflammatory effects of cecropin A (1-8)–Magainin2 (1-12) hybrid peptide analog P5 against Malassezia furfur infection in human keratinocytes. J. Invest. Dermatol. 131: 1677−1683.   DOI
6 Pennanen N, Lapinjoki S, Urtti A, Mönkkönen J. 1995. Effect of liposomal and free bisphosphonates on the IL-1β, IL-6 and TNFα secretion from RAW 264 cells in vitro. Pharm. Res. 12: 916−922.   DOI
7 Rosenberg EW, Noah PW. 1988. The Koebner phenomenon and the microbial basis of psoriasis. J. Am. Acad. Dermatol. 18: 151−158.   DOI
8 Krämer HJ, Podobinska M, Bartsch A, Battmann A, Thoma W, Bernd A, et al. 2005. Malassezin, a novel agonist of the aryl hydrocarbon receptor from the yeast Malassezia furfur, induces apoptosis in primary human melanocytes. Chembiochem. 6: 860−865.   DOI
9 Kim H, Han T-H, Lee S-G. 2009. Anti-inflammatory activity of a water extract of Acorus calamus L. leaves on keratinocyte HaCaT cells. J. Ethnopharmacol. 122: 149−156.   DOI
10 Kim K-Y, Chung H-J. 2000. Flavor compounds of pine sprout tea and pine needle tea. J. Agric. Food Chem. 48: 1269−1272.   DOI
11 Masaki H. 2010. Role of antioxidants in the skin: anti-aging effects. J . Dermatol. Sci. 58: 85−90.   DOI
12 LaDuca JR, Gaspari AA. 2001. Targeting tumour necrosis factor alpha: New drugs used to modulate inflammatory diseases. Dermatol. Clin. 19: 617−635.   DOI
13 Lee E. 2003. Effects of powdered pine needle (Pinus densiflora seib et Zucc.) on serum and liver lipid composition and antioxidative capacity in rats fed high oxidized fat. J. Korean Soc. Food Sci. Nutr. 32: 926−930.   DOI
14 Majdalawieh AF, Hmaidan R, Carr RI. 2010. Nigella sativa modulates splenocyte proliferation, Th1/Th2 cytokine profile, macrophage function and NK anti-tumor activity. J. Ethnopharmacol. 131: 268−275.   DOI
15 Mayser P, Preuss J. 2012. Pityriasis versicolor. Der Hautarzt. 63: 859−867.   DOI
16 Trent JT, Kerdel FA. 2005. Tumor necrosis factor alpha inhibitors for the treatment of dermatologic diseases. Dermatol. Nurs. 17: 97.
17 Round JL, O'Connell RM, Mazmanian SK. 2010. Coordination of tolerogenic immune responses by the commensal microbiota. J. Autoimmun. 34: J220−J225.   DOI
18 Schottelius AJ, Moldawer LL, Dinarello CA, Asadullah K, Sterry W, Edwards CK. 2004. Biology of tumor necrosis factor‐α–implications for psoriasis. Exp. Dermatol. 13: 193−222.   DOI
19 Tengamnuay P, Pengrungruangwong K, Pheansri I, Likhitwitayawuid K. 2006. Artocarpus lakoocha heartwood extract as a novel cosmetic ingredient: evaluation of the in vitro anti‐tyrosinase and in vivo skin whitening activities. Int. J. Cosmet. Sci. 28: 269−276.   DOI
20 Villa FA, Gerwick L. 2010. Marine natural product drug discovery: Leads for treatment of inflammation, cancer, infections, and neurological disorders. Immunopharmacol. Immunotoxicol. 32: 228−237.   DOI
21 Bingham CO. 2002. The pathogenesis of rheumatoid arthritis: pivotal cytokines involved in bone degradation and inflammation. J. Rheumatol. 65: 3−9.
22 Waersted A, Hjorth N. 1984. Pityrosporum orbiculare--a pathogenic factor in atopic dermatitis of the face, scalp and neck? Acta dermato-venereologica. Supplementum. 114: 146−148.
23 Wang H, Gao XD, Zhou GC, Cai L, Yao WB. 2008. In vitro and in vivo antioxidant activity of aqueous extract from Choerospondias axillaris fruit. Food Chem. 106: 888−895.   DOI
24 Yuan G, Wahlqvist ML, He G, Yang M, Li D. 2006. Natural products and anti-inflammatory activity. Asia Pac. J. Clin. Nutr. 15: 143.
25 Bäck O, Faergemann J, Hörnqvist R. 1985. Pityrosporum folliculitis: a common disease of the young and middle-aged. J. Am. Acad. Dermatol. 12: 56−61.   DOI
26 Bergbrant I, Faergemann J. 1990. Presented at the Seminars in dermatology.
27 Gupta AK, Batra R, Bluhm R, Boekhout T, Dawson TL. 2004. Skin diseases associated with Malassezia species. J. Am. Aca. Dermatol. 51: 785−798.   DOI
28 Boncler M, Różalski M, Krajewska U, Podsędek A, Watala C. 2014. Comparison of PrestoBlue and MTT assays of cellular viability in the assessment of anti-proliferative effects of plant extracts on human endothelial cells. J. Pharmacol. Toxicol. Methods. 69: 9−16.   DOI
29 García‐Borrón JC, Solano F. 2002. Molecular anatomy of tyrosinase and its related proteins: beyond the histidine‐bound metal catalytic center. Pigment Cell Res. 15: 162−173.   DOI
30 Jeon MJ, Kim M, Jang H-J, Lee S-W, Kim J-H, Kim H-S, et al. 2012. Whitening effect of Hizikia fusiformis ethanol extract and its fractions. J. Life Sci. 22: 889−896.   DOI
31 Hort W, Mayser P. 2011. Malassezia virulence determinants. Curr. Opin. Infect. Dis. 24: 100−105.   DOI
32 Jeon H-J, Lee K-S, Ahn Y-J. 2001. Growth-inhibiting effects of constituents of Pinus densiflora leaves on human intestinal bacteria. Food Sci. Biotechnol. 10: 403−407.
33 Keddie FM. 1967. Electron microscopy of Malassezia furfur in tinea versicolor. Med. Mycol. 5: 134−137.   DOI
34 Kesavan S, Holland K, Ingham E. 2000. The effects of lipid extraction on the immunomodulatory activity of Malassezia species in vitro. Med. Mycol. 38: 239−247.   DOI
35 Kesavan S, Walters C, Holland K, Ingham E. 1998. The effects of Malassezia on pro-inflammatory cytokine production by human peripheral blood mononuclear cells in vitro. Med. Mycol. 36: 97−106.   DOI
36 Finkel T, Holbrook NJ. 2000. Oxidants, oxidative stress and the biology of ageing. Nature 408: 239−247.   DOI
37 Brunke S, Hube B. 2006. MfLIP1, a gene encoding an extracellular lipase of the lipid-dependent fungus Malassezia furfur. Microbiology 152: 547−554.   DOI
38 Chiba K, Kawakami K, Sone T, Onoue M. 2003. Characteristics of skin wrinkling and dermal changes induced by repeated application of squalene monohydroperoxide to hairless mouse skin. Skin Pharmacol. Physiol. 16: 242−251.   DOI
39 Chua KB, Chua I-L, Chua I, Chong K, Chua KH. 2005. A modified mycological medium for isolation and culture of Malassezia furfur. Malays. J. Pathol. 27: 99−105.
40 Gaitanis G, Bassukas ID, Velegraki A. 2009. The range of molecular methods for typing Malassezia. Curr. Opin. Infect. Dis. 22: 119−125.   DOI