• 보존과학연구
• /
• s.30
• /
• pp.157-170
• /
• 2009

We have evaluated the antifungal activities of oak vinegar to develop a natural biocide for organic cultural heritage. Fungi used in this study were screened from the cultural heritages, Kyujanggak and JanggyeongPanjeon and tested on organic substrates-degrading ability. In the results, 7 species of fungi have produced the extracellular enzymes to degrade CMC, xylan, lignin. Thus, we have used these seven species fungi to investigate the antifungal activity of oak vinegar in this study. In the result, the antifungal activity of oak vinegar indicated positive potencial. Especially, methylene chloride and ethylacetate fractions of the oak vinegar had high activities at the concentration of 5.0mg/disc. In these fractions, many different kinds of compounds such as phenolic and furfural, etc. were analyzed by GC-MS. The experiments indicated that the development of a biocide using natural extracts can have a potential to conserve of organic cultural heritages.

• Bulletin of the Korean Chemical Society
• /
• v.27 no.12
• /
• pp.2023-2027
• /
• 2006

Dibenzyl sulfide was oxidized at the a-carbon to yield benzaldehyde in the presence of $Pd(NO_3)_2$. Oxygen itself could not oxidize the sulfide directly, instead the nitrato ligand of the palladium complex transferred oxygen to dibenzyl sulfide to form benzaldehyde. The X-ray crystal structure of the intermediate complex, cis-[$Pd(NO_3)_2${$S(CH_2C_6H_5)_2$}$_2$], revealed that the nitrato ligand was unidentate. Para-substituted dibenzyl sulfides I, $(YC_6H_4CH_2)_2S $wherein Y = $OCH_3$, $CH_3$, Cl, CN, or $NO_2$, were synthesized and reacted with palladium nitrate, and those with electron-donating substituents (Y = $OCH_3$ and $CH_3$) were good substrates for the oxidation reaction with palladium nitrate. Thus, the reaction mechanism of the oxygen transfer was proposed to include nucleophilic benzylic carbon.

• Bulletin of the Korean Chemical Society
• /
• v.2 no.3
• /
• pp.105-112
• /
• 1981

Various $3{\alpha}$, $5{\alpha}$-cyclocholestan-6${\beta}-yl$ ethers were synthesized from solvolysis reactions of cholesteryl tosylate. ${3\alpha}$, $5{\alpha}$-Cyclocholestan-6${\beta}-yl$ sulfides were the sole products when cholesteryl tosylate was solvolyzed in thiol solvents. Diol solvolysis products were derivatized to aromatic esters, and nuclear magnetic resonance spectroscopic method was used to show that aromatic rings can approach C-18 methyl group and the side chain.

• Research in Plant Disease
• /
• v.30 no.1
• /
• pp.66-77
• /
• 2024

Black wood ear mushroom (Auricularia auricula-judae) is one of the most economically important mushrooms in China, Japan, and Korea. The cultivation of wood ear mushrooms on artificial substrates is more efficient in terms of time and cost compared with their natural growth on trees. However, if the substrate cultivation is infected by fast-growing fungi, the relatively slow-growing ear mushroom will be outcompeted, leading to economic losses. In this study, we investigated the competitive fungal isolates from substrates infected with fast-growing fungi for the cultivation of ear mushrooms in Jangheung and Sunchon, Korea. We collected 54 isolates and identified them by sequencing their internal transcribed spacer region with morphological identification. Among the isolates, the dominant isolates were Trichoderma spp. (92.6%), Penicillium spp. (5.6%), and Talaromyces sp. (1.8%). To find an appropriate eco-friendly biocontrol agent, we used five Streptomyces spp. and Benomyl, as controls against Trichoderma spp. and Penicillium spp. Among the six Streptomyces spp., Streptomyces sp. JC203-3 effectively controlled the fungi Trichoderma spp. and Penicillium spp., which pose a significant problem for the substrates of black wood ear mushrooms. This result indicated that this Streptomyces sp. JC203-3 can be used as biocontrol agents to protect against Trichoderma and Penicillium spp.

• Korean Journal of Ecology and Environment
• /
• v.42 no.1
• /
• pp.124-133
• /
• 2009

Effects of substrates on the relative immigration activities (RIAs) of epilithic diatoms were examined in Sum River, a tributary of South Han River, Korea. Two hundreds of tile substrates coated with 5% agar were deposited at seven study stations (30 tiles per site) for one month from March 7 to April 7, 2008. Water sampling, physico-chemical factor measurement, and diatom analysis were performed in the field and laboratory. Over the study, major epilithic diatom species were Nitzschia amphibia, Navicula subminuscula, Cymbella minuta in both the natural and artificial substrates. Two dominant species, Nitzschia amphibia and Cymbella minuta often found at seven stations. Among all observed taxa, Nav. subminuscula and Caloneis silicula showed the highest RIA, with the value over 15 throughout the study. Regarding the algal morphology, the biraphe type species showed higher RIA than any other morph, while they comprised over 55% of total biomass, indicating a density effect. Additionally, a significant relationship between RIA and 20-day deposited substrate (p<0.05) showed in two diatom species, Nitzschia fonticola, Gomphonema quadripunctatum, which showed relatively high RIAs. These results indicate that the relative immigration activities of epilithic diatom species can be influenced by algal density in the water and substrate, nutrients, and deposited time at least 20 days.

• Journal of Bio-Environment Control
• /
• v.5 no.2
• /
• pp.236-247
• /
• 1996

The main objective of this study was to evaluate the effectiveness of nutrient solutions, substrates, and nutrient solution concentrations in substrate culture of radish(Raphanus sativus L. var. sativus). Cooper's, Hoagland ＆ Arnon' 5, and Yamazaki's solution were used to determine the most suitable nutrient solution in deep flow culture(DFC). In result, Yamazaki's solution treatment showed better results than Hoagland's and Cooper's solution treatments in leaf length, leaf number, shoot and root fresh weights. Cooper's solution was much worse than others. Root shape index were low as 0.6 in all treatments. The selection of suitable was conducted among 14 kinds of substrates which were used commercially, such as sand, perlite and peatmoss, in substrates culture. Sand was the most proper in radish growth and shortened the growth periods. Sand also showed better results then others in leaf length, leaf number, shoot and root fresh weight. On the contrary, radish growth in peatmoss was the worst. Generally, root shape index was higher in substrate than in DFC. In order to investigate the suitable ionic strength in radish, Yamazaki's solution was treated with EC of 0.5, 1.0, 1.5, and 2.0 mS/cm. Generally radish growth above 1.0 mS/cm concentration was good, and the best result was shown in 1.5 mS/cm. Vitamin C contents were not significantly different in the roots of radish grown under 1.0 mS/cm or more. The highest vitamin C content was shown in 0.5 mS/cm, and so was thiocyanate content. Anthocyanin contents increased with the increase of the ionic strength in nutrient solution. Mineral nutrient contents had no significant statistical differences between the treatments, but potassium content was remarkably high in 1.5 mS/cm.

• Journal of Life Science
• /
• v.11 no.2
• /
• pp.92-93
• /
• 2001

Bacillus sp. KYJ 963, which was isolated from Korean salt-fermented anchovy (anchovy-jeot), produces an extracellular ${\beta}$-amylase. The analysis of the digestion products of substrates by thin layer chromatography from the purified protein revealed that the enzyme could not hydrolyze maltose or ${\alpha}$-cyclodextrin. In the amino acid composition analysis, the major characteristic of the ${\beta}$-amylase was the high proportion of amino acids that possess short side chain such as glycine and alanine.

• Advances in nano research
• /
• v.1 no.4
• /
• pp.219-228
• /
• 2013

We report on the preparation of iron pyrite ($FeS_2$) using pulsed electron ablation of two targets, namely, a mixture of sulfur and iron compound target, and a natural iron pyrite target. Thin films of around 50 nm in thickness have been deposited on glass substrates under Argon background gas at 3 mTorr, and at a substrate temperature of up to $450^{\circ}C$. The thin films have been analyzed chemically and examined structurally using x-ray diffraction (XRD), x-ray photoelectron spectroscopy (XPS), and visible Raman spectroscopy. The morphology and thickness of the films have been assessed using scanning electron microscopy (SEM) and visible spectroscopic reflectance. The preliminary findings, using a synthetic target, show the presence of iron pyrite with increasing proportion as substrate temperature is increased from $150^{\circ}C$ to $250^{\circ}C$. The data have not shown any evidence of pyrite in the deposited films from a natural target.

• ALGAE
• /
• v.34 no.4
• /
• pp.303-313
• /
• 2019

Floating seaweed rafts are a surface-pelagic habitat that serve as substrates for benthic flora and fauna. Since 2008, Sargassum horneri clumps have periodically invaded the Korea Strait. In this study, the polymerase chain reaction-free small-organelles enriched metagenomics method was adopted to identify the species of epibiotic eukaryotes present in floating S. horneri fronds. A total of 185 species were identified, of which about 63% were previously undetected or unreported in Korean waters. The rafts harbored a diverse assemblage of eukaryotic species, including 39 Alveolata, 4 Archaeplastida, 95 Opisthokonts, 4 Rhizaria, and 43 Stramenopiles. Of these 185 taxa, 48 species were found at both Sargassum rafts collection stations and included 24 Stramenopiles, 17 Alveolata, and 7 Opisthokonts. Among these, the highest proportion (50%) of species was photo-autotrophic in basic trophic modes, while the proportion of phagotrophic, osmo- or saprotrophic, and parasitic modes were 43.8%, 4.2%, and 2.1%, respectively. This study demonstrates the contribution of floating Sargassum rafts as dispersal vectors that facilitate the spread of alien species.

• KSBB Journal
• /
• v.27 no.6
• /
• pp.341-346
• /
• 2012

Indican (indoxyl-${\beta}$-D-glucoside) is a colorless natural compound and can be used as a precursor for the production of indigo. This production step only require an enzyme, ${\beta}$-glucosidase, that readily screened from microbial resource by using selective media supplemented with indican as a sole carbon source. Agrobacterium tumefaciens was well grown in this media and thus presumed to produce a related enzyme. The corresponding gene, encoding a protein with a calculated molecular mass of 51 kDa, was cloned and overexpressed as MBP fusion proteins. The purified enzyme was determined to be a dimer and showed the maximum activity for indican at pH 7.0 and $40^{\circ}C$. The kinetic parameters for indican, Km and Vmax, were determined to be 1.4 mM and 373.8 ${\mu}M/min/mg$, respectively. The conversion yield of indican into indigo using this enzyme was about 1.7-1.8 folds higher than that of previously isolated enzyme from Sinorhizobium meliloti. Additionally, this enzyme was able to hydrolyze various ${\beta}$-1,4 glycoside substrates.