• Korean Journal of Plant Resources
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• v.25 no.1
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• pp.7-13
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• 2012

This study was conducted to determine the effects of scarification temperature, and sulfuric acid treatments on seed germination of white lotus collected from the Muan districts, Jeonnam in Korea. Without scarification, white lotus seeds were not germinated at all at 15 days after seeding. However, seeds sacrificed at basal parts showed 100% germination rate at $25^{\circ}C$ 6 days after seeding. All the seeds floated (0.90 g) and soaked (1.18 g) in the water were completely germinated. Especially, the lotus seeds soaked in the water at $25^{\circ}C$ showed high germination rate. Seeds treated with 80% sulfuric acid for 40-160 hours were germinated completely within 6 days after seeding. No difference in seed germination rate of white lotus stored up to 0, 12 and 24 months after harvest was observed. Overall results would be useful means for propagation and production of white lotus.

• Weed & Turfgrass Science
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• v.5 no.4
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• pp.196-202
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• 2016

With increasing LM soybean import, the concern about unintentional gene flow from LM soybean to wild soybean and consequential weedy risk has been growing. Therefore, we conducted this study to characterize seed traits including germination of hybrids resulted from gene flow from LM soybean to wild soybean in comparison with their parents, LM soybean and wild soybean. Pollen-donor LM soybean seeds were much greater and heavier (about 15.0 g of 100 seed weight) than F2 hybrid (5.7 g), while pollen-recipient wild soybean and F1 hybrid seeds were smallest and lightest (about 2.5 g). F2 hybrid was brown, intermediate between yellow LM soybean seed and black wild soybean seed. These findings indicate that F1 hybrid seeds show similar characteristics with wild soybean, while F2 hybrid seeds show intermediate color and size between two parents. F2 hybrid seed showed intermediate traits between two parents in germination and dormancy rates, which were 35% and 65%, respectively. LM soybean showed no dormancy, while wild soybean showed greater than 90% dormancy. This finding indicates that F2 hybrid show intermediate characteristics in seed germination with high dormancy trait, suggesting a potential weediness of hybrids resulted from gene flow from LM soybean to wild soybean.

• Korean Journal of Pharmacognosy
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• v.12 no.1
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• pp.12-14
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• 1981

${\beta}-Sitosteryl-3-O-{\beta}-D-glucopyranoside$ was isolated from the seeds of Plantago asiatica (Plantaginaceae). The assignments of $^{13}C$ NMR spectra of ${\beta}-sitosterol$ and ${\beta}-sitosteryl-3-O-{\beta}-D-glucopyranoside$ were made by comparing with $^{13}C$ NMR spectra of cholesterol and $cholesteryl-3-O-{\beta}-D-glucopyranoside$. Our data indicate that the revision of previous $^{13}C$ NMR spectral assignment is needed.

• Korean Journal of Pharmacognosy
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• v.7 no.2
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• pp.95-97
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• 1976

Saponin was distributed in all tissues of Phytolacca americana and P. esculenta. Phytolaccoside E was a major saponin, but the callus tissues induced from stems of P. americana contained phytolaccoside B as a major component. Saponin contents in leaves and stems were almost similar to those in roots. Two unknown compounds, $C_{32}H_{50}O_{4}$ (triterpenoid) and $C_{18}H_{22}O_{5}{\cdot}H_2O$ (polyphenol) were isolated from seeds of both plant.

• Korean Journal of Pharmacognosy
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• v.9 no.3
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• pp.139-144
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• 1978

Total methanol extract of Plantago asiatica seeds showed significant hepatotonic activities against animal model of hepatitis. The methanol extract was further fractionated with petroleum ether, chloroform, n-butanol and water. Among these fractions, methanol extract and water and chloroform fractions exhibited hepatonic activities, whereas petroleum ether and n-butanol fractions appeared to give rise a rather toxicity. Measurements of serum GOT, GPT and duration of sleeping time induced by hexobarbital after $CCl_4$ intoxication indicated that water fraction showed most potent activities.

• Natural Product Sciences
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• v.27 no.2
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• pp.68-77
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• 2021

Silymarin is a standardized extract obtained from the seeds and fruits of Silybum marianum L., or commonly called milk thistle, a member of Carduus marianum family that contains mix of flavonolignans. Some epidemiological and preclinical studies revealed that S. marianum L. has been used for herbal remedies for centuries for its pharmacological activity. In this review, pharmacological studies in vitro and in vivo of silymarin are discussed thoroughly stressing on antioxidant, antimicrobial, antiviral, and anti-carcinogenic aspects of silymarin. In addition, the protective influences of silymarin on some organs such as heart, liver, bone, and neuron tissue are reviewed as well. This review would be useful for further study regarding the potential of natural plant, notably silymarin, and its therapeutic potential in the prevention and treatment of diseases.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.18 no.6
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• pp.201-214
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• 2015

This study was conducted to identify forest topsoil's usefulness from July 2013 to Oct 2014. Generally, there are abundant seeds in topsoils and it can be used at restoration works. Mt. GARIWANG is famous for biodiversity in Korea. This study was aimed to find out the suitable utilization methods of natural topsoils for restoration works at sky-slopes construction sites. Test beds was made to identify suitable collecting times and suitable topsoil thickness. The main results are summarized as follows. First, Mt. GARIWANG have diverse species and in topsoils we can find lots of burried seeds which can be used at restoration works. Second, according to indoor experiment, as the depth of topsoil used thicker, the number of plants and coverage rates were increased. Also, there were no distinct differences in coverage rates between 5cm and 8cm thick topsoil plots and it can be possible to use at least 3cm thick topsoils even thou lower coverage rates. Third, as the results of research about indoor and outdoor experimental plots, there were difference in plant emergency. If we use the stabilizer to protect soil erosion, then we can get more increased results at sloped outdoor experimental plots. Fourth, based on environmental impact assessment, there was 389 species in whole region of Mt. GARIWANG. Through this seed bank experiments, we can find 23 varieties, including 4 tree varieties and 19 herbaceous varieties. We can find lots of native herbaceous species from topsoils. Fifth, this research was done at the specific area of Mt. GARIWANG and used limited topsoils. But we monitoring more longer periods, then there will be more useful outcomes. Finally by researching topsoils of Mt. GARIWANG, we can find diverse native plants. Thus, we must reuse natural topsoils of Mt. Gariwang for restoration of original vegetation.

• BMB Reports
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• v.33 no.2
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• pp.112-119
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• 2000

Three kinds of serine protease inhibitors, members of the Bowman-Birk trypsin inhibitor, were purified from Dolichos lablab seeds and named Dolichos protease inhibitor 1, 2 and 3 (DI-1, DI-2 and DI-3), respectively. Each inhibitor showed a single band with gel mobility at around 15.9, 12.1 and 14.6 kDa on 20% SDS-PAGE under reducing conditions. To characterize inhibitory specificity, the inhibition constant (Ki) for these inhibitors was measured against several known serine proteases. All three Dolichos protease inhibitors (DI-1, DI-2 and DI-3) inhibited the activity of trypsin and plasmin, but had no effect on thrombin and kallikrein (either for human plasma kallikrein or for porcine pancreas kallikrein). DI-1 inhibited chymotrypsin most effectively (Ki = $3.6{\times}10^{-9}\;M$), while DI-2 displayed inhibitory activity for porcine pancreatic elastase (Ki = $6.2{\times}10^{-8}\;M$). Pre-treatment of the 33 mg/kg of DI-mixture (active fractions from $C_{18}$ open column chromatography that included DI-1, DI-2 and DI-3) inhibited the induction of pseudomonal elastase-induced septic hypotension and prevented an increase in bradykinin generation in pseudomonal elastase-treated guinea pig plasma. Also, the increase of kallikrein activity, by injection of pseudomonal elastase, was inhibited by the pretreatment of the DI-mixture in a guinea pig. Since the DI-mixture had no inhibitory effect on kallikrein activity when Z-Phe-Arg-MCA was used as a substrate in vitro, its inhibitory activity in the pseudomonal elastase-induced septic hypotension model might not be due to a direct inhibition of plasma kallikrein in the activation cascade of the Hageman factor and prekallikrein system. These results suggest that the Dolichos DI-mixture might be used as an inhibitor in pathogenic bacterial protease-induced septic shock.

• Korean Journal of Environmental Agriculture
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• v.34 no.4
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• pp.260-267
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• 2015

BACKGROUND: The current study was conducted to examine the species specific accumulation of Cd and Pb in 11 crop species (Soybean, Sesame, Corn, Polished rice, Carrot, Potato, Garlic, Spring onion, Chinese leek, Red pepper, Eggplant), through cultivating them under the same condition with metal contaminated soils.METHODS AND RESULTS: Eleven crop species were cultivated in three different soils contaminated with Cd and Pb and harvested. Edible parts of each crop was pretreated and analyzed to determine Cd and Pb concentrations, and subsequently bioconcentration factors (BCFs) were calculated. In general, the crops of which seeds are used as food showed high concentrations of both Cd and Pb. For instance, Cd concentrations in crops cultivated in Soil A was in the order of soybean (0.432 mg kg^-1) > sesame (0.385) > polished rice (0.176) > carrot (0.116) > corn (0.060) > red pepper > (0.047) > potato (0.044) > egg plant (0.025) > garlic (0.023) > spring onion (0.016) > Chinese leek (0.011). BCFs showed the same order.CONCLUSION: From this study, it can be conclude that seeds plants should not be cultivated in Cd and Pb contaminated soils to secure food safety from metal contaminated soils.

• The Journal of Natural Sciences
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• v.11 no.1
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• pp.99-103
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• 1999

This study was carried out to use as the basic data for breeding by evaluating parthenocarpy with 12 tomato lines and 17 allied species. Fruit set of open pollination plots was over 90% in 11 lines(CLN430-85-13-5 etc), 9.1-50% in 10 lines(LA1306 etc.), and the rest no fruit set. Fruit set after emasculation resulted highly 51.4% in CLN431-85-13-12, 53.9% in CLN425-130-8-1, 66.7% in CLN435-185-4-9, and 72.2% in Ventura, respectively. But fruit set in other tomato lines resulted under 50% and 17 allied species(LA1306 etc) resulted no effect of fruit set. Fruits other emasculation had no seeds and fruits after open pollination had 10-70 seeds per fruit. This result of this work showed that 3 lines, CLN435-185-4-9, CLN425-130-8-1, and CLN431-85-13-12, resulted in good effectiveness on the evaluation of parthenocarpy in tomatoes.