• 생약학회지
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• 제22권4호
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• pp.246-248
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• 1991

The methanolic extracts of forty-two medicinal plants were screened for direct effects on the isolated ileum and uterus and for anticholinergic and oxytocic effects on the respective organ preparation.

• 대한약학회:학술대회논문집
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• 대한약학회 2003년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2-1
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• pp.88-88
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• 2003

The syntheses of different types of stilbenoid libraries have been studied recently. In these courses, the screening of the generated natural product-mimic focused libraries led to the identification of the novel lead compounds for human cytochrome P450 (CYP) lAs, melanin production, and sortase A. A library of trans-stilbene derivatives was prepared through a new efficient solution pahse synthetic pathway and their inhibitory activities were evaluated on human cytochrome P450s(CYP) 1A1, 1A2, and 1B1 to find a potent and selective CYP1 inhibitor. (omitted)

• Animal cells and systems
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• 제6권4호
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• pp.317-322
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• 2002

To improve conventional yeast one-hybrid screening, we have developed an efficient mammalian one-hybrid system that allows rapid isolation of com-plementary DNAs which are able to induce human p14$^{ARF}$. tumor suppressor gene. A 1.5 kb promoter region of p14$^{ARF}$ was fused to EGFP to generate ARF promoter-EGFP reporter vector. This reporter plasmid was stably trans-fected into NIH3T3 cells for generation of reporter cell line. When the reporter cell line was infected with E2F-1 together with excess amounts of empty vector, the cells that received the positive modulator were readily identifiable by green fluorescence using FACS. The GFP-positive cells were cloned directly from the cultured cells and expanded in bulk culture. The genomic DNAs from GFP-positive cells were prepared and the CDNA insert in integrated retroviral genome was recovered by PCR using primers annealing to the retroviral vector sequences flanking the insert-cloning site. This system should be useful for efficient screening of expression CDNA libraries in mammalian cells to identify novel upstream regulators for spe-cific genes by one-hybrid interaction.ion.

• 한국입자에어로졸학회지
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• 제10권1호
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• pp.19-25
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• 2014

In line with the expected price reduction of natural gas associated with the introduction of shale gas, it is expected that the optimal power mix for the electric power generation be changed. In this study, the reconfigured power mix is estimated with the varying natural gas price by using the Screening Curve Method (SCM). It is found that about 3% and 9% coal in the overall power mix is replaced with natural gas if the natural gas price falls 20% and 40% of the current price, respectively. It is also found that the reconfigured power mixes would provide the reduction of the emissions of air pollutants which are equivalent to 369 and 807 MUS$.

• 한국재난정보학회 논문집
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• 제18권2호
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• pp.252-260
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• 2022

연구목적: 기후변화 등으로 인해 자연재해의 빈도 및 강도가 증가하고 있어, 원자력발전소에 영향을 주는 외부사건이 발생할 수 있다. 원자력발전소는 자연재해, 인공재해 등 외부사건으로부터 안전해야 한다. 따라서 원자력발전소에 발생할 수 있는 외부사건을 식별하여야 하며, 원자력발전소에 큰 영향을 줄 수 있는 외부사건을 선별하여야 한다. 본 연구에서는 외부사건을 식별 및 선별하는 방법의 연구동향을 소개한다. 연구방법: 원자력발전소의 확률론적 안전성 평가를 위한 외부사건의 식별 및 선별에 관한 문헌조사를 실시하였다. 또한 국내외 외부사건의 식별 및 선별에 관한 규정을 조사하였다. 연구결과: 원자력발전소의 외부사건 영향분석의 비용을 최소화하고자 외부사건을 식별 및 선별하는 연구가 이루어지고 있다. 각 연구는 관점에 따라 차이를 보이지만 공통적으로 식별과정에서는 원자력발전소 부지에서 발생할 수 있는 모든 사건을 식별하고자 하며, 선별과정에서는 정성적 기준과 정량적 기준을 바탕으로 외부사건을 선별한다. 결론:기후변화 등으로 인하여 자연재해의 강도가 변화하고 있어, 원자력발전소에 영향을 주는 외부사건을 식별 및 선별하는 과정이 중요해지고 있다. 따라서 본 논문에서는 외부사건을 식별과 선별하는 방법에 대해 조사하여 정리하였다. 국내의 경우 원자력발전소의 확률론적 안전성 평가를 위한 외부사건의 식별과 선별에 관한 연구가 미미한 실정이다. 또한 외부사건 정량적 선별과정에서 수행하는 '경계분석'과 '명백히 보수적인 분석' 방법에 관한 연구가 필요할 것으로 보인다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권9호
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• pp.3817-3825
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• 2015

Background: The human protein methyl-transferase DOT1L catalyzes the methylation of histone H3 on lysine 79 (H3K79) at homeobox genes and is also involved in a number of significant processes ranging from gene expression to DNA-damage response and cell cycle progression. Inhibition of DOT1L activity by shRNA or small-molecule inhibitors has been established to prevent proliferation of various MLL-rearranged leukemia cells in vitro, establishing DOT1L an attractive therapeutic target for mixed lineage leukemia (MLL). Most of the drugs currently in use for the MLL treatment are reported to have low efficacy, hence this study focused on various natural compounds which exhibit minimal toxic effects and high efficacy for the target receptor. Materials and Methods: Structures of human protein methyl-transferase DOT1L and natural compound databases were downloaded from various sources. Virtual screening, molecular docking, dynamics simulation and drug likeness studies were performed for those natural compounds to evaluate and analyze their anti-cancer activity. Results: The top five screened compounds possessing good binding affinity were identified as potential high affinity inhibitors against DOT1L's active site. The top ranking molecule amongst the screened ligands had a Glide g-score of -10.940 kcal/mol and Glide e-model score of -86.011 with 5 hydrogen bonds and 12 hydrophobic contacts. This ligand's behaviour also showed consistency during the simulation of protein-ligand complex for 20000 ps, which is indicative of its stability in the receptor pocket. Conclusions: The ligand obtained out of this screening study can be considered as a potential inhibitor for DOT1L and further can be treated as a lead for the drug designing pipeline.

• 한국작물학회지
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• 제49권spc1호
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• pp.217-223
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• 2004

• 한국동물학회:학술대회논문집
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• 한국동물학회 1999년도 공동 춘계학술대회
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• pp.80.2-80
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• 1999

No Abstract, See Full Text

• 대한약학회:학술대회논문집
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• 대한약학회 2005년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.208-209
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• 2005

• 약학회지
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• 제43권3호
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• pp.342-350
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• 1999

The antibacterial activity against Propionibacterium acnes (P. acnes), $5{\alpha}-reductase$ inhibition and comedolytic effects are the important pharmacological target sites of antiacne drughs. We previously reported on the antibacterial activities against P. acnes by natural products. In the present study the screening of $5{\alpha}-reductase$ inhibition and comedolytic effects from natural products were performed. Seven natural products such as Angelica koreana, Sophora flavescens, Prunus persica, Bombyx mori, Areca catechu, Galla rhois and Gleditschia koraiensis perfectly inhibited the activity of $5{\alpha}-reductase$ at the concentration of 0.01% (w/v). Sixteen natural products which were shown to have the potent antibacterial activities against P.acnes or $5{\alpha}-reductase$ inhibition activities were assayed for the comedolytic test. In the results of comedolytic effects on experimentally-induced comedones (EIC), Sophora flavescens showed the strongest comedolytic effect on EIC, and Polygonum cuspidatum and Angelica koreana showed stronger comedolytic effects on EIC than azelaic acid used for a positive control at the concentration of 3% (w/v). These results suggest that several natural products including Sophora flavescens can be developed as noble antiacne agents.