• Korean Journal of Ecology and Environment
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• v.44 no.1
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• pp.42-57
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• 2011

We described the above-ground plant species composition and measured a range of soil physico-chemical properties and the composition and size of the soil seed bank in the remnant natural vegetations on the flood plains of the Han River within Seoul, South Korea. We used analysis of variance and multivariate analyses to analyse the data and S${\o}$rensen's similarity index to compare the composition of the vegetation and seed banks. The soils were circum-neutral and composed of mainly sand and silt fractions with a very limited clay component; a gradient based on sand/clay proportions was identified. The soil seed banks varied markedly between- and within-sites and had much greater species diversity than the above-ground vegetation. Two of the major dominants in the vegetation (Miscanthus saccariflorus and Phragmites australis) were found at very low densities in the seed bank. The site differences appeared to be correlated with the sand-clay gradient, suggesting that the soil properties differentially affected seed inputs into the soil, or that the processes than controlled sediment deposition during floods was also important in differentially affecting seed deposition. Lastly, there was relatively little similarity between the vegetation, dominated mainly by perennials, and the seed bank which contained a relatively large proportion of annuals and biennials. This result suggests that after disturbance caused by flooding there is the potential for many other species to colonize. This may impinge on the regeneration potential of the sites and cause concern for the future conservation of these important remnants of natural vegetation.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.35 no.6
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• pp.608-613
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• 2002

Experimental transplantation of Eklonia stoionifera to natural rock was carried out using adhesive Blue. A plant was attached to an architecture tile ($10\times$10 cm) with the glue, and then the tile was attached to natural rock with underwater glue. Within one month, the tile were fixed to natural rock with an attachment rate of $75\%$, and 5. stoionifera grew up with an insertion rate of $66.7\%$ on the tile. After two months, number of E. stolonifera on the tile decreased. However, the attached plants were regenerated by new stolons, maintaining good growing condition. After seven months,5. stoloaifera increased 6 to 71 individuals. These results indicate that regeneration of plants was mainly affected by the recruitment of stolen around the plant.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2001.11b
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• pp.5-10
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• 2001

It is necessary for governmental supporting polices to collect. management and maintain the genetic resource with respects of diversity of useful natural resource plant and domestic variety for plant industry. There are the main tasks of functional plant industry to develope the techniques of systemic management of genetic resources with sufficient life because of having the key of human's fate with life and death and being the valuable thing to be Investigation. collection and introduction of genetic resource with natural resource plants. Agriculture after WTO is putting an emphasis on the development of functional plants with highly competitiveness In world market under long term and organizing plants Genetic resource of plant in the official institution are maintaining and managing to be possible the regeneration and reproduction if necessary. and utilizing whoever need them.

• Korean Journal of Environmental Biology
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• v.25 no.2
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• pp.158-167
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• 2007

A study conducted in NaeSorak Mt. from 1996 to 2006 shows some interesting results indicating general characteristics of natural forests, regeneration and structural patterns on fir stands. Those fir stands could have been confused as climax forests, which is a short-sighted from human-centered view on those stands. A classic theory of succession, which has been frequently applied to analyse the changes in forest ecosystem changes, failed to provide further understanding of fir stands. To comprehend the complicated and diverse process, a concept of ecosystem circulation need be introduced to the theory. This is a main idea of 'patch dynamics' theory developed from 'mosaic cycle' theory.

• Journal of Forest and Environmental Science
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• v.17 no.1
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• pp.1-17
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• 2001

Forming a part of "Cooperative Practical Study for the Modernization of the Management of National Forest", this study was conducted to provide overall ecological information for the natural regeneration of major tree species on the basis of community structural attributes in the deciduous forest ecosystem. Followings are summarized characteristics of the natural regeneration for the selected tree species. Betula costata : Although large number of seeds are dispersed by wind, they require mineral soils to germinate. Thick litter layer could be an obstacle to germinate. After germination, the seedling requires large amount of light for successful establishment. Acer mono : Characterized by high shade tolerance and weak drought resistance, the seedling should be overcasted with more than 50% of canopy coverage. High stand density should be maintained to produce good quality of timber. The potential of coppice may be high. Ulmus laciniata : Since this species needs high rate of troll moisture and light, around 60% of canopy coverage should be maintained to retain moisture and incoming light. The competition with other vegetation should be removed for the favor of successful seedling establishment. Fraxinus mandshurica : This species requires moist mineral soils to germinate. After germination, the seedling needs large amount of light and moisture for successful establishment. Site preparation should be applied to reduce competition with weedy vegetation. Fraxinus rhynchophylla : Interval of large seed crops may be highly varied. Thick litter layer could be an obstacle to germinate. Site preparation should be applied to reduce competition with weedy vegetation so as to achieve successful seedling establishment. Quercus mongolica : Including the difficulty of seed supply by the consumption, thick litter layer and mountain bamboo cover could be the obstacle to germinate. More than 50% of relative light intensity is necessary to achieve successful seedling establishment. Kalopanax pictus : Thick litter layer could be an obstacle to germinate. The seedling needs large amount of light and moisture for successful establishment. Abies holophylla : In spite of high shade tolerance, the growth rate in sapling stage may be extremely slow. Cornus controversa : Seeds (drups) are consumed and dispersed by animals, tending to be not sufficient in seed supply. This species requires large amount of light for successful germination and seedling establishment. Tilia amurensis : The difficulty of seed supply might be expected with low seed purity and double dormancy. Since thick litter layer could be an obstacle to germinate, the species requires moist mineral soils for successful germination. The potential of coppice may be extremely high.

• Journal of Korean Society of Forest Science
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• v.112 no.3
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• pp.280-289
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• 2023

Diverse native tree species are distributed in Korean forests; however, information on their regeneration strategies is limited. This study analyzed seed rain from 2011 to 2013, seedling emergence, seedling survival, lifespan distribution, and the relationship between seedling mortality and herbaceous vegetation for 3 years, from 2012 to 2014, to understand the natural regeneration and early survival characteristics of Fraxinus rhynchophylla Hance in natural broad-leaved forests. Seed rain and emerged seedling density significantly differed among the years (p < .05). An increase in seed rain was followed by an increase in seedling emergence in the following year. Seed rain in 2013 and seedling emergence in 2014 showed a significant correlation (p < .05), confirming the trait of F. rhynchophylla seeds that germinate in the following year after seed production. Seedlings emerged in late spring and early summer. Of the seedlings, 78% emerged in the early summer of 2014. Most seedlings died immediately after emergence. Of the seedlings that emerged in August 2012, 56% died within 2 months, and 38% of those that emerged in July 2014 died within 1 month. Shade and competition against ground vegetation chiefly affected the seedling survival of F. rhynchophylla. Fraxinus rhynchophylla showed mast seeding, with a large number of seed production in a particular year. A surge of seedling emergence followed in the early summer of the next year. However, most seedlings died in the same year of emergence. Fraxinus rhynchophylla has a high seedling mortality, but mast seeding and the resulting high seedling emergence produce a few survived seedlings, enabling the continuation of the F. rhynchophylla population. Efforts and research on the natural regeneration of native tree species are required to effectively manage natural forests in Korea.

• Korean Journal of Environmental Biology
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• v.29 no.4
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• pp.345-352
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• 2011

We investigated 12 plots using Herles' diversity of structure feature calculation method, which is based on the Shannon-Weaver-Index for estimation of species diversity. This study sought to facilitate a more systematic understanding of the structure of the forest stands in the Korean fir natural forest in Naeseorak. Although the change in the forest structure is dependent on the change in phase, factors of the natural forest were confirmed by associating individual structure features. As shown in the results of diversity of structure features, the diversity of the structure of the fir tree natural forest in Naeseorak was relatively low. The association between species diversity and overall factors (diversity) related with the change in the structure was found to be weak. The association between the number of trees and the diversity of forest structure stands was moderate, showing that the higher the number of trees, the less diverse the forest structure is. In most of the investigated plots, stem volume and volume of dead tree were associated with the height of natural regeneration, but these were not associated with other factors. Height of natural regeneration was found to be correlated to stand density, crown area and crown class, whereas tree height and BHD did not have any association with other factors. Overall, the results of the investigation are helpful in understanding the change in the structure of Korean fir natural forest. Further investigation with more plots is required.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
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• v.32 no.4
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• pp.295-307
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• 2006

Styela clava, called non-native tunicate or sea squirt, is habitat which include bays and harbors in Korea and several sites in the sea faced world. We fabricate cellulose membrane nerve conduit (CMNC) from this native sea squirt skin, and evaluate the capacity of promoting peripheral nerve regeneration in the rat sciatic nerve defect model. After processing the pure cellulose membrane from the sea squirt skin as we already published before, CMNC was designed as a non-tubular sheet with 14 mm length and 4 mm width. Total eleven male Spraque-Dawley rats (12 weeks, weighing 250 to 300g) were divided into sham group (n=2), silicone tube grafted control group (n=3) and experimental group (n=6). Each CMNC grafted nerve was evaluated after 4, 8 and 12 weeks in the experimental group, and after 12 weeks, sciatic function was evaluated with sciatic function index (SFI) and gait analysis, and histomorphology of nerve conduit and the innervated tissues of sciatic nerve were all examined using image analyzer and electromicroscopic methods in the all groups. The regenerated axon and nerve sheath were found only in the inner surface of the CMNC after 4 weeks and became more thicker after 8 and 12 weeks. In the TEM study, CMNC grafted group showed more abundant organized myelinated nerve fibers with thickened extracellular matrix than silicone conduit grafted group after 12 weeks. The sciatic function index (SFI) and ankle stance angle (ASA) in the functional evaluation were $-47.2{\pm}3.9$, $35.5^{\circ}{\pm}4.9^{\circ}$ in CMNC grafted group (n=2) and $-80.4{\pm}7.4$, $29.2^{\circ}{\pm}5.3^{\circ}$ in silicone conduit grafted group (n=3), respectively. And the myelinated axon was 41.59% in CMNC group and 9.51% in silicone conduit group to the sham group. The development of a bioactive CMNC to replace autogenous nerve grafts offers a potential and available approach to improved peripheral nerve regeneration. As we already published before, small peptide fragment derived from the basement membrane matrix proteins of squirt skin, which is a kind of anchoring protein composed of glycocalyx, induced the effective axonal regeneration with rapid growth of Schwann cells beneath the inner surface of CMNC. So the possibilities of clinical application as a peripheral nerve regeneration will be able to be suggested.

• Proceedings of the Korean Society for Agricultural Machinery Conference
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• 2017.04a
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• pp.109-109
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• 2017

Cirsium setidens nakai belonging to cirsium has been reported to have various physiological activities including anticancer activity because it contains polyphenols, dietary fiber, minerals and vitamins. Despite these positive positive efficacies, however, no studies have studied cirsium setidens nakai products as biomaterials such as cellular metabolism and bone formation. Thus, the aim of this study was evaluate of osteogenesis differentiation a natural material extracted from cirsium setidens nakai. The natural materials in this studys in this studywere created by 40% ethanol extraction process and then dried. FabricatedFabricatedpowders were added to a medium at various concentrations (0.01, 0.05, 0.1, 0.2, and $0.25{\mu}g/mL$), and pure medium was used as a control. The natural material caused positive increases in cell metabolic activity and mineralized bone formation without cytotoxicity. In addition, we observed higher expression of genes such as ALP, BSP, Runx2 and COL1 in cirsium setidens nakai treatment cells. As a result, this study produced and investigated cirsium setidens nakai extracts and the natural materials showed potential biomaterials. In this research indicated that the cirsium setidens nakai extracts might have promising applications in areas of agricultural, biological and food engineering as a biomaterial.

• Macromolecular Research
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• v.17 no.9
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• pp.682-687
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• 2009

Nanofibrous membranes of poly(D,L-lactic acid)/chitin blend were prepared by electro spinning for a barrier of guided tissue regeneration. A miscible solution was obtained by the blending chitin-salt complex into 1-methyl-2-pyrrolidone solution of poly(D,L-lactic acid). The properties of the blend were examined for nanofibrous fabrication. The viscosity of the blend solution was increased significantly due to chain entanglement despite the low ratio of chitin to poly(D,L-lactic acid). An interaction between two polymeric compositions was confirmed by Fourier transform infrared spectroscopy. X-ray diffraction detected an appreciably ordered microstructure in the nanofiber of the blend. A membrane of thinner nanofibers was fabricated by electro spinning the chitin blend. The permeability of the membranes was examined using bioactive model compounds.