• Journal of Wetlands Research
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• v.18 no.4
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• pp.456-464
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• 2016

Korea established an inventory of 1,916 sites of inland wetlands during a nationwide investigation from 2000 to 2010. If inland wetlands is included in or near various protected areas designated by the government, it can be selected as a wetland to be managed with priority. This study evaluated the aspect of management of inland wetlands by analyzing the correlation between locations of national protected areas and inland wetlands. As a result, it was shown that a considerable percentage of current inland wetland was located in areas that were designated and managed as protected areas by the government, as they had a high value of natural environment protection (527 sites, 31.61 %). When the range was widened to a radius of 1 km for protected areas, 959 sites were included and 57.53 % of sites were located in or nearby the protected areas. Among them, 46.79 % of sites were distributed up to or within a 1 km radius of waterside areas and rivers; it accounted for 81.33 % of wetlands located in protected areas. Therefore, it was found that locations of current inland wetlands were mostly in contact with rivers. The results of overlay analysis were classified into high, medium and low; the correlation of location with inland wetlands was analyzed through the analysis of separation distance of various protected areas. The number of wetlands located in areas of a 'high' value of protection was 998 (59.87 %); 289 sites (17.34 %) were distributed in areas of a 'low' value of protection. This implies that these wetlands are located in artificial areas and are more exposed to environmental pressures. Thus, these wetlands could be determined as inland wetlands, which we considered for the establishment of measures to prevent damage.

• Journal of Plant Biotechnology
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• v.44 no.2
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• pp.171-177
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• 2017

A callus-mediated regeneration protocol for sea-milkwort, an endangered coastal plant species in South Korea, is reported here. The explants of in vitro-plantlets generated from a node culture revealed distinguishable responses in callus induction depending on genotype, explant source, light condition, and 2,4-D concentration. Especially, continuous darkness exclusively facilitated callus induction from explants prior to other treatments. The calli initiated on the media with 2,4-D ranging from 0.1 mg/L to 3.0 mg/L in the dark vigorously proliferated when subcultured on the same media in continuous darkness. Given 1.0 mg/L zeatin in addition to darkness to the calli of the 'Pistachio' genotype, normal adventitious shoots were only regenerated from nodular structures that formed earlier from the calli at the frequency of 24.4 percent. Regenerated shoots easily grew into plantlets with roots and green color on a phytohormone-free MS medium under lighted condition, that were used for node culture as plant materials. Node culture effectively multiplied plantlets in accordance with protocol by Bae et al. (2016). Acclimatized plantlet clusters developed mature plant clusters under inland environment, followed by flowering the following April. Results were merged with node culture protocol suggested by Bae et al. (2016), which, as an in vitro propagation system for sea-milkwort, may contribute to natural habitat restoration.

• The Journal of the Petrological Society of Korea
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• v.27 no.3
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• pp.121-138
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• 2018

We have examined the petrotectonic setting and magmatic evolution from petrochemical characteristics of major and trace elements for the Cretaceous volcanic rocks in and around the Mireukdo Island. The volcanic rocks, can be devided into Jusasan, Unmunsa, Yokji and Saryang subgroups on the ascending order, are classified as basalt, basaltic andesite, andesite, dacite and rhyolite on TAS diagram. Petrochemical data show that the rocks are calc-alkaline series, and suggest that erupted earlier medium-K series and later high-K series. The volcanic rocks provide a case in which the calc-alkaline magma are formed, not only from separate protoliths, but following separate paths from source to surface. Earlier and later subgroups take different paths to the surface respectively, and are emplaced in the shallow crust as a series of discrete magma chambers through the volcanic processes. After emplacement, each chamber evolves indepently through fractional crystallization with a little assimilation of wall rock. The volcanic rocks have close petrotectonic affinities with orogenic suite and subduction-related volcanic arc. The rhyolitic magma can be derived from calc-alkaline andesitic magma by fractional crystallization with crustal assimilation, which may be derived from a partial melt of peridotite in the upper mantle.

• Journal of Life Science
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• v.28 no.8
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• pp.955-961
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• 2018

Although much research has focused on various bioactive substances in starfish, research on microorganisms isolated from starfish is lacking as compared with other natural products. In this study, we investigated bacterial communities in the gut of red starfish (Certonardoa semiregularis) in Jeju Island. In total, 103 bacterial strains were isolated using marine agar and R2A medium. The isolated strains were analyzed and identified using the 16S rRNA gene sequence. Based on an analysis of this gene sequence, the 103 isolated bacteria were classified into four major groups: Proteobacteria (93%: Alpha-proteobacteria, 24.8%; Beta-proteobacteria, 4%; Gammaproteobacteria, 65%) Bacteroidetes (4%), Actinobacteria (2%), and Firmicutes (1%). In addition, the isolates were divided into seven classes (Actinobacteria, Flavobacteria, Bacilli, Sphingobacteria, Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria), 15 orders, 19 families, and 24 genera. A phylogenetic analysis revealed two strains, Lysobacter sp. and Pedobacter sp., with similarity of 97.55% and 97.58%, respectively. As the similarity in the 16S rRNA gene sequence was 98% or less compared to previously identified bacteria, the two strains may possibly be classified as a new genus or species. We suggest that additional studies, including biochemical and morphological tests, should be performed to identify the new candidate strains.

• Journal of Wetlands Research
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• v.22 no.1
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• pp.31-38
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• 2020

Weirs are to secure amount water of streams and drop structures are to enhance stabilization of stream bed and bridges are to connect isolated region, which are called stream crossing structures. In the stream design criteria, directions for minimum size of structures are suggested to secure stability of stream crossing structures. However the sizes of almost all existing weirs and drop structures are not satisfied with the stream design criteria and only 22 percent of the peirs of bridges are satisfied. To enhance hydraulic stability of existing weirs and drop structures, it is required that the ratio of bed protection to apron should be above 3.3. According to factual survey of structures in the sample streams, it has been shown that the longitudinal slope of rapid works with 1:20 is the most reasonable to design velocity if existing weirs and drop structures are rehabilitated into rapid works. It has been known that violating freeboard and span length of piers should make existing bridges reconstructed or removed. However, comprehensive review including deterioration level of bridges, special regulation for span length, etc. should be considered to determine rehabilitation plan of bridges. In this study, a procedure has been suggested to improve hydraulic stability of weirs, drop structures and piers of bridges. Sound environment of stream and reduction of natural disaster could be achieved by improving stability of cross structures, which could be obtained by governmental budget and active stream management including observance of design criteria.

• Development and Reproduction
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• v.12 no.2
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• pp.125-132
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• 2008

Melatonin (N-acetyl-5-methoxytryptamine), a major hormone of pineal gland in vertebrates, is known to be associated with regulation of the dynamic physiological functions in general and has some functions on reproduction in the ovarian follicles in particular. And its antioxidant properties as a scavenger are also reported. The aim of this study was to investigate the effect of melatonin on the in vitro maturation of mouse germinal vesicle (GV)-stage oocytes. Oocyte maturation, apoptosis, and mRNA expression of melatonin receptor were analyzed in the cumulus cell-enclosed oocytes (CEOs) cultured with melatonin for 18 h. The CEOs were obtained from 3 wk-old ICR female mice cultured in media with 0, 0.1 nM, 10 nM, or 1,000 nM melatonin for 18 h. And then the extrusion of the first polar body was assessed to evaluate the maturation rate. The apoptosis and mRNA expression of melatonin receptor (Mtnr1-a and Mtnr1-b) in cumulus cells of each group were measured by TUNEL assay, ELISA, and real time RT-PCR after in vitro maturation(IVM). The addition of melatonin in the IVM medium significantly improved nuclear maturation of the mouse GV oocytes and the highest maturation rate were obtained from the group treated with 1,000 nM melatonin. Apoptosis was not detected in IVM oocytes, but detected in cumulus cells. And cumulus cells treated with 1,000 nM melatonin exhibited significantly lower apoptosis. In the group treated with 1,000 nM melatonin, the expression of melatonin receptor mRNA was decreased in CEOs. In conclusion, melatonin has a potentially important role for regulating oocyte maturation and reduces the apoptosis of cumulus cells in vitro.

• Korean Journal of Medicinal Crop Science
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• v.14 no.3
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• pp.153-157
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• 2006

Floret flowering of Orostachys japonicus, a monocarpic and short day plant, should be controlled to continue cultivating. The study was done to examine the effect of transplanted seedling size (large, 18 leaves; medium, 13 leaves; small, 8 leaves) on growth, morphological characters, and flowering. Night-break treatment of 2 hours at midnight were done on August 25 and afterward samples were taken every 2 weeks. Growth, morphological and flowering related characters were measured from each sample. With larger seedling transplanted, plant height, inflorescence length, number of leaves including bracts and stem diameter were increased. Leaves and bracts, stem, root, shoot and total dry weights were increased as transplanted seedlings became larger. Although formed florets were inclined with larger transplanted seedling, they were not flowered because of the night-break treatment, meaning that the larger transplanted seedlings, the more economic yield.

• Korean Journal of Medicinal Crop Science
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• v.15 no.4
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• pp.246-250
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• 2007

Rhodiola sachalinensis A. Bor is a Chinese herb containing the natural compound salidroside, which has been known to possess medical properties such as enhancing bodies' ability to survive in adverse environments and extending human life. To improve a metabolite production from Rhodiola sachalinensis callus cultures, the concentrations of macro nutrients were investigated. To investigate the salidroside production in Rhodiola sachalinensis callus cultures, we analyzed salidroside content in each callus which was cultured in 30 $m{\ell}$ of $2\;{\times}\;B_5$ liquid medium for 4 weeks. The optimal concentrations of macro nutrients for salidroside production ($67.96\;{\pm}\;3.41\;mg/{\ell}$) were found to be 99 mM $KNO_3$, 1 mM $(NH_4)_2SO_4$, 2 mM $NaH_2PO_4{\cdot}2H_2O$, 2 mM $CaCl_2{\cdot}2H_2O$ and 1 mM $MgSO_4{\cdot}7H_2O$. From these results, we determined the more upgraded culture condition for industrial production of salidroside.

• Journal of Hydrogen and New Energy
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• v.25 no.6
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• pp.648-655
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• 2014

This paper describes the continuing effort to develope a single acting free-piston Stirling engine/alternator combination for use of the household cogeneration. Free piston Stirling engines(FPSE) use variations of working gas pressure to drive mechanically unconstrained reciprocating elements. Stirling cycle free-piston engines are driven by the Stirling thermodynamic cycle which is characterized by an externally heated device containing working gas that is continuously re-used in a regenerative, reversible cycle. The ideal cycle is described by two isothermal process connected by two constant volume processes. Heat removed during the constant volume cooling process is internally transferred to the constant volume heating process by mutual use of a thermal storage medium called the regenerator. Since the ideal cycle is reversible, the ideal efficiency is that of Carnot. Free-piston Stirling engine is have no crank and rotating parts to generate lateral forces and require lubrication. The FPSE is typically comprised of two oscillating pistons contained in a common cylinder. The temperature difference across the displacer maintains the oscillations, and the FPSE operate at natural frequency of the mass-spring system. The power is generated from a linear alternator. The purpose of this paper is to describe the design process of the single acting free-piston Stirling engine/alternator. Electrical output of the single acting free-piston Stirling engine/alternator is about 0.95 kW.

• Journal of the Korean Society of Food Science and Nutrition
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• v.38 no.10
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• pp.1466-1470
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• 2009

This study was focused on the development of anticariogenic edible films using pullulan containing grapefruit seed extract (GFSE), polylysine or propolis. According to the result of antimicrobial activity (disc diffusion method) of GFSE, polylysine and propolis against Streptococcus mutans, antimicrobial pullulan film was produced by adding grapefruit seed extract. The optimum combination of pullulan and sorbitol (plasticizer) was 10$\sim$15% (w/v) and 40$\sim$50% of pullulan (w/w), respectively. Minimum concentration of grapefruit seed extract for growth inhibition of Str. mutans was 50 ppm in medium. Formulation of antimicrobial pullulan films containing grape seed extract was established and these results evidently showed potential for commercial application.