• 대한기계학회:학술대회논문집
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• 대한기계학회 2001년도 추계학술대회논문집B
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• pp.306-311
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• 2001

Effect of boundary layer thickness on the flow characteristics around a rectangular prism has been investigated by using a PIV(Particle Image Velocimetry) technique. Three different boundary layers(thick, medium and thin)were generated in the Atmospheric Boundary Layer Wind Tunnel at Pusan National University. The thick boundary layer having 670mm thickness was generated by using spires and roughness elements. The medium thickness of boundary layer$(\delta=270mm)$ was the natural turbulent boundary layer at the test section with fully long developing length(18m). The thin boundary layer with 36.5mm thickness was generated by on a smooth panel elevated 70cm from the wind tunnel floor. The Reynolds number based on the free stream velocity and the height of the model was $7.9{\times}10^3$. The mean velocity vector fields and turbulent kinetic energy distribution were measured and compared. The effect of boundary layer thickness is clearly observed not only in the length of separation bubble but also in the reattachment points. The thinner boundary layer thickness, the higher turbulent kinetic energy peak around the model roof. It is strongly recommended that the height ratio between model and approaching boundary layer thickness should be a major parameter.

• 수산해양교육연구
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• 제28권3호
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• pp.645-652
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• 2016

At the initial stage of propulsion shaft design, in line with shaft alignment, an intensified consideration of lateral vibration is needed to verify its operational safety. Recently the alignment problem affecting the lateral vibration has been becoming issues. However, the theoretical method of forced lateral vibration analysis is not cleary established so far and it is about to simply limited among the classification societies and international standards to avoid the blade natural frequency resonance cpm outside of ${\pm}20%$ of engine rpm at MCR. On the other hand, longer center distance between each support bearing shows an affirmative result normally in shaft alignment analysis whereas the blade order resonance speed may cause lowering near the limitation in the aspect of lateral vibration. Therefore, it is required careful attention to engineers as described above. As a method to solve the problem, it is mainly considered that remove forward stern tube bearing. In this paper, based on a medium size container ship case, theoretical study was carried out in the context of the forward stern tube bearing. The various effects and functions of forward stern tube bearing are reviewed and evaluated. Then an guidance note to lead the conclusion is proposed.

• Journal of Ginseng Research
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• 제16권1호
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• pp.24-30
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• 1992

Extracts of Panax ginseng root significantly induced tolerance of Fusarium oxysporum to heavy metal, mecury, as the fungal mycelial growth was less inhibited by mercury chloride on potato dextrose medium(PDA) amended with ginseng root than on the PDA with no ginseng amendment. The most favorable concentration of ginseng root powder in detoxification of mercury chloride was 1%. The induced tolerance of F. oxysporum to mercury chloride appeared to be rather due to absorption of ginseng components, and was not related to stimulation of mycelial growth of the fungus per so by ginseng treatment. Ginseng components responsible for inducing tolerance of the fungus to mercury were involved in the water fraction of the ginseng root extract, although the water fraction had no effect on enhancement of the mycelial growth on the medium without mercury chloride. The hexane fraction of ginseng root extract, by which the mycelial growth was stimulated, was not related to the inducement of the tolerance to mercury chloride. However, more tolerance to mercury chloride was noted in PDA with both the water and hexane fractions combined than with either of the two fractions. Six-year-old ginseng roots were more effective in detoxification of mercury chloride than 4-year-old ginsng roots, and American ginseng (P quinquifolium) had no or little effect on inducing tolerance of the fungus to mercury chloride. This method may be used to screen other natural materials for test in the detoxification of mercury chloride.

• KSBB Journal
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• 제11권1호
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• pp.1-7
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• 1996

은행 (Ginkgo biloba L.) 세포의 최적 배양 조건과 유 용 성분의 생산 가능성을 규명하기 위해 은행의 각 부위로부터 캘러스를 유도, 배양하였으며 유용 성분을 검출, 분석하였다. 기내에서 무균적으로 발아시킨 은행의 유식물체로부터 잎과 줄기, 그리고 은행 성숙 종자로부터 배 (embryo)를 적출하여 2mg/ P N NAA와 5mg/ P kinetin이 첨가된 WP 고형배지에 서 캘러스를 유도시켰다. 세포 기원 별로 선발된 3 종류의 캘러스를 각각 1mg/P NAA와 O.lmg/ P kinetin이 첨가된 MS 액체배지( 3 % sucrose, pH 5 5.8)에서 16시간 광주기로 계대배양하였을 때 엽록 소를 다량 함유한 green callus를 형성 하였고, 배 유래의 캘러스가 가장 빠른 성장을 나타냈다. TLC 및 EMS를 이용하여 flavonoid 및 그 전구물질 퉁 의 유용물질을 확인한 바, 앞에서 검출되지 않은 flavonoid의 전구물질 퉁이 캘러스에서 확인되었다.

• Journal of Microbiology
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• 제34권4호
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• pp.327-333
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• 1996

Yeast, like many other microbes, encounters large variations in ambient pH in their natural environments. Microorganisms capable of growing over a wide pH range require a versatile, efficient pH homeostatic mechanism protecting intracellular processes against extremes of pH. In several organisms, fusions to the bacterial lacZ gene have been extremely useful for the identification of genes expressed at different time during the life cycle or under different growth conditions. In this study, using the lacZ gene screening system, we surveyed a large number of yeast strains with lacZ insertion to identify genes regulated by pH. A yeast genomic library was constructed and inserted with lacZ by a shuttle mutagenesis procedure. The yeast transformants were individually picked up with a toothpick, replica-plated, and grown in alkaline pH medium. Among the 35,000 colonies screened, 10 candidate strains were identified initially by the $\beta$-gal assay. We finally confirmed two yeast strains carrying the genes whose expression are strictly dependent on pH of growth medium. One of the fusions showing a 10-fold induction in expression level in response to alkali pH was selected and further characterized. The pH-regulated gene was cloned by inverse PCR and a partial sequence of the gene was determined. Identification and characterization of the gene is currently under investigation.

• 대한미생물학회지
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• 제35권4호
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• pp.309-316
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• 2000

This study was focused on the isolation of pathogenic Vibrio species, V. vulnificus and V. parahaemolyticus from marine environment from May to July of 1999. Isolation sites were coast near by Pusan and Daechon. The results obtained were as follows: 1. Seventy strains of V. parahaemolyticus and 19 strains of V. vulnificus were isolated from a total of 120 specimens. 2. Nineteen strains of V. vulnificus did not fermented arabinose and salicin but fermented lactose and cellobiose. All of V. parahaemolyticus isolates did not fermented lactose and cellobiose. 47 strains of V. parahaemolyticus fermented arabinose but 53 strains did not fermented salicin. 3. V. vulnificus and V. parahaemolyticus isolates showed three different API index numbers with 5046105 and 4346107 dominant. 4. V. vulnificus did not grow on 0% and 8% NaCl containing medium. V. parahaemolyticus grew on 8% NaCl containing medium. 5. V. vulnificus isolates and V. parahaemolyticus revealed different outer membrane protein profiles on SDS-PAGE.

• Clinical and Experimental Reproductive Medicine
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• 제13권2호
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• pp.201-206
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• 1986

These experiments were conducted to know whether RNA syntheis is involved in the cumulus expansion and oocyte maturation of mouse cumulus-oocyte complexes in vitro. Mouse cumulus-oocyte complexes(COC's) were cultured in the presence of cordycepin, an inhibitior of RNA synthesis and its effect on the cumulus expansion and oocyte maturation were examined. The results were as follows. 1. Continuous presence of cordycepin in the medium(200${\mu}g/ml$) inhibited the HCG-induced cumulus cell expansion of mouse complexes. This inhibition was reversible. 2. When the COC'S were preincubated with different concentration of cordycepin plus HCG for 3 hours and then transferred to the plain medium, the HCG induced cumulus expansion was suppressed at $100{\mu}g/ml$ of cordycepin. 3. When the COC'S were cultured with cordycepin after HCG stimulation(3hrs), the cumulus expansion were not suppressed by cordycepin. 4. Oocyte meiotic maturation did not appear to be affected by cordycepin. The data presented here imply that RNA synthesis is involved in the cumulus expansion process and that mouse oocytes are more resistant to RNA synthesis inhibitor than cumulus cells.

• 한국주거학회:학술대회논문집
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• 한국주거학회 2005년도 추계학술대회 논문집
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• pp.193-200
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• 2005

Living room in modern housing accepts social behavior of modem family realized diversified life and the core of the housing space. So living room in the housing space is composed of many various components, handled very carefully in a color plan. This study is purposed on proposing basic data for color planing of the living room in an apartment, especially a sold housing accommodating the social change. As a result of understanding the color image of a apartment living room for the last 5 years, phenomenon of using natural color which makes people feel comfortable is common with durability. Also the image of space composition which usually called in modern, classic and dandy is appeared with various coloration easily in a medium-large or large living room, So these show the various demand of high-level or medium-level inhabitants. For an actual proof, basic data for research about the color image of a apartment living room in korea have to be accumulated constantly. After that the research which can take concrete shape and visualize the change of the color image of korean apartment living room will revitalize.

• 대한전기학회논문지:전기기기및에너지변환시스템부문B
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• 제52권9호
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• pp.434-441
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• 2003

This paper deals with the temperature characteristics according to the cooling medium and the duct size in model transformers. For the analysis and the temperature-rise tests, two 400kVA model transformers have been manufactured. One has been filled with the alpha oil as the cooling medium and constructed the duct sizes of $3\textrm{mm}$ and $5\textrm{mm}$ in the low-voltage and high-voltage windings respectively. The other has been filled the beta oil and the duct sizes were $4\textrm{mm}$ and $6\textrm{mm}$. The temperature-rise tests have been performed by the back-to-back method and the load factor has been controlled the range of 90%∼130%. The temperature values have been measured by the thermocouple and from the sixteen points in each transformer. A commercial CFD program "FLUENT" has been used for the analysis of temperature distribution. The geometry of transformer has been modeled to 3-dimensional by using the hybrid calculation mesh including the radiator. And also, the natural convection velocity has been measured at the oil top position, and compared with the calculated results.

• 한국미생물·생명공학회지
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• 제23권3호
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• pp.263-268
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• 1995

A screening was performed to isolate exoploysaccharide-producing microorganisms, which synthesized specific exopolysaccharide for the substitutive of commercial polysaccharides, from natural sources. Soil bacterium, one of 378 mucoid isolates, was finally selected as potential producer of polysaccharides which made the culture broth very viscous and thus examined in detail for optimal medium composition. Isolated strain was identified as Xanthomonas sp. EPS- 1 from the results of morphological and biochemical characteristics. The composition of optimal medium for exopolysaccharide production was as follows: 50 g sucrose, 1.5 g peptone, 2 g KH$_{2}$PO$_{4}$, 2 g MgSO$_{4}$, -7H$_{2}$O, 3 g NaCl, 0.05 g CaCO$_{3}$, 0.07 g FeSO$_{4}$-7H$_{2}$O and 0.05 g MnSO$_{4}$-7H$_{2}$O in 1 liter of distilled water. From the experiments of temperature and pH dependence, the optimal conditions for exopolysaccharide biosynthesis seemed to be 30$\circ$C and 8.0, respectively. About 14.9 gram of maximum exopolysaccharide per liter was obtained at the initial pH 8.0, 30$\circ$C and 250 rpm in a flask culture. The exopolysaccharide EPS-1 had such potential as an emulsifying agent and a gelling agent in comparision with commercial exopolysaccharide.