• 제목/요약/키워드: natural medium

검색결과 1,272건 처리시간 0.028초

Mucin modifies microbial composition and improves metabolic functional potential of a synthetic gut microbial ecosystem

  • Mabwi, Humphrey A.;Komba, Erick V.G.;Mwaikono, Kilaza Samson;Hitayezu, Emmanuel;Mauliasari, Intan Rizki;Jin, Jong Beom;Pan, Cheol-Ho;Cha, Kwang Hyun
    • Journal of Applied Biological Chemistry
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    • 제65권1호
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    • pp.63-74
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    • 2022
  • Microbial dysbiosis in the gut is associated with human diseases, and variations in mucus alter gut microbiota. Therefore, we explored the effects of mucin on the gut microbiota using a community of 19 synthetic gut microbial species. Cultivation of these species in modified Gifu anaerobic medium (GAM) supplemented with mucin before synthetic community assembly facilitated substantial growth of the Bacteroides, Akkermansia, and Clostridium genera. The results of 16S rRNA microbial relative abundance profiling revealed more of the beneficial microbes Collinsella, Bifidobacterium, Ruminococcus, and Lactobacillus. This increased acetate levels in the community cultivated with, rather than without (control), mucin. We identified differences in predicted cell function and metabolism between microbes cultivated in GAM with and without mucin. Mucin not only changed the composition of the gut microbial community, but also modulated metabolic functions, indicating that it could help to modulate microbial changes associated with human diseases.

Statistical Optimization of Medium Components for the Production of Prodigiosin by Hahella chejuensis KCTC 2396

  • Kim, Sung-Jin;Lee, Hong-Kum;Yim, Joung-Han
    • Journal of Microbiology and Biotechnology
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    • 제18권12호
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    • pp.1903-1907
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    • 2008
  • Prodigiosin is a natural red pigment with algicidal activity against Cochlodinium polykrikoides, a major harmful red-tide microalga. To increase the yield of prodigiosin production by Hahella chejuensis KCTC 2396, significant medium components were determined using a two-level Plackett-Burman statistical design technique. Among 12 components included in basal medium, $NaHCO_3$, ${Na}_{2}{SiO}_{3}$, ${NH_4}{NO_3}$, ${Na}_{2}{SO}_{4}$ and $CaCl_2$ were determined to be important for prodigiosin production. The medium formulation was finally optimized using a Box-Behnken design as follows: 1% sucrose; 0.4% peptone; 0.1 % yeast extract; and (g/l): NaCl, 20.0; ${Na}_{2}{SO}_{4}$, 9.0; $CaCl_2$, 1.71; KCl, 0.4; and (mg/l): ${H_3}{BO_3}$, 10.0; KBr, 50.0; NaF, 2.0; $NaHCO_3$, 45.0; ${Na}_{2}{SiO}_{3}$, 4.5; ${NH_4}{NO_3}$, 4.5. The predicted maximum yield of prodigiosin in the optimized medium was 1.198 g/l by the Box-Behnken design, whereas the practical production was 1.495 g/l, which was three times higher than the basal medium (0.492 g/l).

Free vibration analysis of trapezoidal Double Layered plates embedded with viscoelastic medium for general boundary conditions using differential quadrature method

  • S. Abdul Ameer;Abbas Hameed Abdul Hussein;Mohammed H. Mahdi;Fahmy Gad Elsaid;V. Tahouneh
    • Steel and Composite Structures
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    • 제50권4호
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    • pp.429-441
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    • 2024
  • This paper studies the free vibration behavior of trapezoidal shaped coupled double-layered graphene sheets (DLGS) system using first-order shear deformation theory (FSDT) and incorporating nonlocal elasticity theory. Two nanoplates are assumed to be bonded by an interlayer van der walls force and surrounded by an external kelvin-voight viscoelastic medium. The governing equations together with related boundary condition are discretized using a mapping-differential quadrature method (DQM) in the spatial domain. Then the natural frequency of the system is obtained by solving the eigen value matrix equation. The validity of the current study is evaluated by comparing its numerical results with those available in the literature and then a parametric study is thoroughly performed, concentrating on the series effects of angles and aspect ratio of GS, viscoelastic medium, and nonlocal parameter. The model is used to study the vibration of DLGS for two typical deformation modes, the in-phase and out-of-phase vibrations, which are investigated. Numerical results indicate that due to Increasing the damping parameter of the viscoelastic medium has reduced the frequency of both modes and this medium has been able to overdamped the oscillations and by increasing stiffness parameters both in-phase and out-of-phase vibration frequencies increased.

Soyasaponins from Soybean Flour Medium for the Liquid Culture of Ganoderma applanatum

  • Lee, So-Young;Kim, Ju-Sun;Shim, Sang-Hee;Kang, Sam-Sik
    • Bulletin of the Korean Chemical Society
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    • 제32권10호
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    • pp.3650-3654
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    • 2011
  • Two new unusual soyasaponins named 6"-O-methyldehydrosoyasaponin I(7) and desglucosylsoyasaponin $A_1$ (10) along with eight known saponins, dehydrosoyasaponin IV (1), dehydrosoyasaponin III (= impatienoside A) (2), soyasaponin III (3), dehydrosoyasaponin II (= soyasaponin Bg) (4), soyasaponin II (5), dehydrosoyasaponin I (= soyasaponin Be) (6), soyasaponin I (8), and kudzusaponin $SA_3$ (9), were isolated as their methyl esters and identified from the liquid culture of G. applanatum. Their structures were determined by chemical and spectroscopic analyses including 1D- and 2D-NMR as well as by comparison of their spectroscopic data with those of the reported in literatures. Although dehydrosoyasaponin IV was identified by LC-MS/MS method from soy protein isolate, this is the first report of the isolation of this compound. Dehydrosoyasaponin III (2) and kudzusaponin $SA_3$ (9) were also isolated for the first time from soybean. The presence of soyasaponins in Ganoderma species seems to be unusual feature. Thus, we presumed that compounds 1-10 might all be derived from the defatted soybean flour which was added to the culture medium as a nitrogen source.

Electron Firehose Instabilities in High-β Intracluster Medium

  • Kim, Sunjung;Ha, Ji-Hoon;Ryu, Dongsu;Kang, Hyesung
    • 천문학회보
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    • 제44권2호
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    • pp.55.2-55.2
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    • 2019
  • The firehose instability is driven by a pressure anisotropy in a magnetized plasma when the temperature along the magnetic field is higher than the perpendicular temperature. Such condition occurs commonly in astrophysical and space environments, for instance, when there are beams aligned with the background magnetic field. Recently, it was argued that, in weak quasi-perpendicular shocks in the high-β intracluster medium (ICM), shock-reflected electrons propagating upstream cause the temperature anisotropy. This electron temperature anisotropy can trigger the electron firehose instability (EFI), which excites oblique waves in the shock foot. Scattering of electrons by these waves enables multiple cycles of shock drift acceleration (SDA) in the preshock region, leading to the electron injection to diffusive shock acceleration (DSA). In the study, the kinetic properties of the EFI are examined by the linear stability analysis based on the kinetic Vlasov-Maxwell theory and then further investigated by 2D Particle-in-Cell (PIC) simulations, especially focusing on those in high-β (β~100) plasmas. We then discuss the basic properties of the firehose instability, and the implication of our work on electron acceleration in ICM shock.

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자색고구마 습관성캘러스로부터 안토시아닌 고생합성 세포주 선발 (Selection of High Anthocyanin-Producing Cells from Habituated Callus Derived from Purple Sweetpotato)

  • 박혜정;김윤실;박현용
    • Journal of Plant Biotechnology
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    • 제34권1호
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    • pp.19-24
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    • 2007
  • 자색고구마 (Ipomoea batatas cv. Jami) 잎 조직배양으로 유도된 캘러스는 계대배양시 생장량이 감소하였다. 빠르고 간편하게 생장하는 습관성캘러스를 $1{\mu}M\;2,4-D$ 첨가된 MSBM에서 계대배양 없이 6개월간 지속배양하여 선발하였다. 습관성캘러스는 생장조절제가 첨가되지 않는 MSBM의 고체, 액체 배지에서 계대배양 횟수의 제한 없이 빠르게 지속생장이 가능하였다. 암조건의 액체배양 6 개월 후에는 자색생합성 부위가 생겼으며, 이를 2년 여간 분리하여 자색습관성세포괴를 선발하였다. 안토시아닌 함량은 자미의 괴근에서 추출되는 1.5 mg/ml에 근접한 1.0 mg/ml으로 나타났다. 자색습관성 세포괴는 안토시아닌 기내대량에 효과적 세포주로 사용 될 수 있을 것으로 기대한다.