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http://dx.doi.org/10.3839/jabc.2022.009

Mucin modifies microbial composition and improves metabolic functional potential of a synthetic gut microbial ecosystem  

Mabwi, Humphrey A. (Natural Product Informatics Research Center, KIST Gangneung Institute of Natural Products)
Komba, Erick V.G. (Department of Microbiology, Parasitology, and Biotechnology, College of Veterinary Medicine and Biomedical Sciences, Sokoine University of Agriculture)
Mwaikono, Kilaza Samson (Department of Science and Laboratory Technology, Dar es Salaam Institute of Technology)
Hitayezu, Emmanuel (Natural Product Informatics Research Center, KIST Gangneung Institute of Natural Products)
Mauliasari, Intan Rizki (Natural Product Informatics Research Center, KIST Gangneung Institute of Natural Products)
Jin, Jong Beom (Natural Product Informatics Research Center, KIST Gangneung Institute of Natural Products)
Pan, Cheol-Ho (Natural Product Informatics Research Center, KIST Gangneung Institute of Natural Products)
Cha, Kwang Hyun (Natural Product Informatics Research Center, KIST Gangneung Institute of Natural Products)
Publication Information
Journal of Applied Biological Chemistry / v.65, no.1, 2022 , pp. 63-74 More about this Journal
Abstract
Microbial dysbiosis in the gut is associated with human diseases, and variations in mucus alter gut microbiota. Therefore, we explored the effects of mucin on the gut microbiota using a community of 19 synthetic gut microbial species. Cultivation of these species in modified Gifu anaerobic medium (GAM) supplemented with mucin before synthetic community assembly facilitated substantial growth of the Bacteroides, Akkermansia, and Clostridium genera. The results of 16S rRNA microbial relative abundance profiling revealed more of the beneficial microbes Collinsella, Bifidobacterium, Ruminococcus, and Lactobacillus. This increased acetate levels in the community cultivated with, rather than without (control), mucin. We identified differences in predicted cell function and metabolism between microbes cultivated in GAM with and without mucin. Mucin not only changed the composition of the gut microbial community, but also modulated metabolic functions, indicating that it could help to modulate microbial changes associated with human diseases.
Keywords
16S rRNA gene sequencing; Mucin; Short chain fatty acids; Synthetic gut microbiome;
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