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http://dx.doi.org/10.5010/JPB.2007.34.1.019

Selection of High Anthocyanin-Producing Cells from Habituated Callus Derived from Purple Sweetpotato  

Park, Hye-Jeong (Department of Biology, College of Natural Science, Chosun University)
Kim, Yoon-Sil (Department of Biology, College of Natural Science, Chosun University)
Park, Hyeon-Yong (Department of Biology, College of Natural Science, Chosun University)
Publication Information
Journal of Plant Biotechnology / v.34, no.1, 2007 , pp. 19-24 More about this Journal
Abstract
Callus induction using leaf of purple sweetpotato (PSP) was decreased when subcultured. So we selected habituated callus in MS medium supplemented with $1{\mu}M\;2,4-D$ (2,4-dichlorophenoxyacetic acid) after 6 months of cultures (without subculture). It grew faster and easier than any other callus. It was able to proliferate in MS hormone free solid and liquid medium without any growth regulators and subculture limits. During subculture in liquid medium, a purple mottled spot formed in one of habituated cell aggregates without any treatment. This purple cell aggregates were carefully separated from habituated cell aggregates, and then subcultured by selecting purple cell aggregates for more than 2 years to be isolated. The color value of the pigment extracted of culture was 1.0 mg/mL, which was close to that of a pigment extracted from storage root, which was 1.5 mg/mL. This purple cell aggregates could therefore be used for the industrial mass production of anthocyanin.
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1 Zhong JJ, Yoshida M, Fujiyama K, Seki TTY (1993) Enhancement of anthocyanin production by Perilla frutescens cells in a stirred bioreactor with internal light irradiation. J Ferment Bioeng 75: 299-303   DOI   ScienceOn
2 Sakamoto K, Iida K, Sawamura K, Hajiro K, Asada Y, Yoshikawa, T FT (1994) Anthocyanin production in cultured cells of Aralia cordata Thumb. Plant Cell Tiss Org Cult 36: 21-26   DOI
3 Binns A, Meins F (1973) Habituation of Tobacco Pith Cells for Factors Promoting Cell Division Is Heritable and Potentially Reversible. Proc Natl Acad Sci USA 70: 2660-2662
4 Chen PN, Chu SC, Chiou HL, Chiang CL, Yang SF, Hsieh YS (2005) Cyanidin 3-glucoside and peonidin 3-glucoside inhibit tumor cell growth and induce apoptosis in vitro and suppress tumor growth in vivo. Nutr Cancer 53: 232-243   DOI   ScienceOn
5 Decendid A, Merillon J (1996) Condensed tannin and anthocyanin production in Vitis vinifera cell suspension culture. Plant Cell Rep 15: 762-765   DOI   ScienceOn
6 Edahiro J, Nakamura M, Seki M, Furusaki S (2005a) Enhanced accumulation of anthocyanin in cultured strawberry cells by repetitive feeding of L- phenylalanine into the medium. J Biosci Bioeng 99: 43-47   DOI   ScienceOn
7 Edahiro J, Nakamura M, Seki M, Furusaki S (2005b) Enhanced Accumulation of Anthocyanin in Cultured Strawberry Cells by Repetitive Feeding of L-Phenylalanine into the Medium. J BIOSCI BIOENG 99: 43-47   DOI   ScienceOn
8 Fox JE (1963) Growth factor requirements and chromosome number in tobacco tissue cultures. Physiol Plant 16: 793-803   DOI
9 Francis FJ (1989) Food colorants: anthocyanins. Crit Rev Food Sci Nutr 28: 273-314   DOI   ScienceOn
10 Kim HJ, Tsoy I, Park JM, Chung JI, Shin SC, Chang KC (2006) Anthocyanins from soybean seed coat inhibit the expression of TNF-alpha-induced genes associated with ischemia/reperfusion in endothelial cell by NF-kappaB-dependent pathway and reduce rat myocardial damages incurred by ischemia and reperfusion in vivo. FEBS Lett 580: 1391-1397   DOI   ScienceOn
11 Konczak-Islam I, Yoshinaga M, Nakatani M, Yamakawa O, Terahara N (2000) Establishment and characteristics of an anthocyanin-producing cell line from sweetpotato storage root. Plant Cell Rep 19: 472-477   DOI
12 Park HJ, Ahn YS, Jeong BC, Park HY (2003) Plant regeneration derived from leaf disk cultures in purple sweetpotato. Kor J Plant Biotech 30: 245-249   DOI   ScienceOn
13 Meyer J, Pepin M, Smith M (2002) Anthocyanin production from Vaccinium pahalae: l imitations of the physical microenvironment. J Biotechnol 93: 45-57   DOI   ScienceOn
14 Montoro P, Tuberoso CI, Piacente S, Perrone A, De Feo V, Cabras P, Pizza C (2006) Stability and antioxidant activity of polyphenols in extracts of Myrtus communis L. berries used for the preparation of myrtle liqueur. J Pharm Biomed Anal 41: 1614-1619   DOI   ScienceOn
15 Mori T, Sakurai M, Shigeta J, Yoshida K, Konda T (1993) Formation of anthocyanins from cell cultured from different parts of strawberry plants. J Food Sci 58
16 Pischke MS, Huttlin EL, Hegeman AD, Sussman MR (2006) A transcriptome-based characterization of habituation in plant tissue culture. Plant Physiol 140: 1255-1278   DOI   ScienceOn
17 Sakamoto K, Iida K, Sawamura K, Hajiro K, Asada Y, Yoshikawa T, Furuya T (1993) Effects of nutrients on anthocyanin production in cultured cells of Aralia cordat. Phytochem 33: 357-360   DOI   ScienceOn
18 Tsuda T, Horio F, Kitoh J, Osawa T (1999) Protective effects of dietary cyanidin 3-O-beta-D-glucoside on liver ischemia-reperfusion injury in rats. Arch Biochem Biophys 368: 361-366   DOI   ScienceOn
19 Vitrac X, Larronde F, Krisa S, Decendit A, Deffieux G, Merillon JM (2000) Sugar sensing and Ca2+-calmodulin requirement in Vitis vinifera cells producing anthocyanins. Phytochemistry 53: 659-665   DOI   ScienceOn
20 Zhang W, Curtin C, Kikuchi M, Franco C (2002) Integration of jasmonic acid and light irradiation for enhancement of anthocyanin biosynthesis in Vitis vinifera suspension cultures. Plant Science 162: 459-468   DOI   ScienceOn
21 Zhong JJ, Yoshida M, Fujiyama K, Seki T, Yoshida T (1993) Enhancement of anthocyanin production by Perilla frutescens cells in a stirred bioreactor with internal light irradiation. J Ferment Bioeng. 75: 299-303   DOI   ScienceOn
22 Bridle P, Timberlake C (1997) Anthocyanins as natural food colours-selected aspects. Food Chem 58: 103-109   DOI   ScienceOn
23 Koukalova B, Fojtova M, Lim KY, Fulnecek J, Leitch AR, Kovarik A (2005) Dedifferentiation of tobacco cells is associated with ribosomal RNA gene hypomethylation, increased transcription, and chromatin alterations. Plant Physiol 139: 275-286   DOI   ScienceOn
24 Park KI, Choi JD, Hoshino A, Morita Y, Iida S (2004) An intra genic tandem duplication in a transcriptional regulatory gene for anthocyanin biosynthesis confers palecolored flowers and seeds with fine spots in Ipomoea tricolor. Plant J 38: 840-849   DOI   ScienceOn
25 Meins FJ, Foster R (1985) Reversible, cell-heritable changes during the development of tobacco pith tissues. Dev Biol 108: 1-5   DOI   ScienceOn