• 한국의류산업학회지
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• 제21권1호
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• pp.112-119
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• 2019

This study had a purpose of further investigating and developing the usage of the eco-friendly functional dye that has physiological and pharmacological functions. This study was implemented complex dyeing method which used both natural dyes such as Gardenia jasminoides J.Ellis colorant, which are the yellow dyes that have high brightness and low chroma, and Xanthium strumarium L. extract. The silk fabrics were used to dye in various methods. They were also used to investigate functionalities dyed fabrics by various treating method, such as their color fastness, antibacterial activities and deodorization, the following were obtained. The fabrics that were dyed with herbs had decreasing brightness as the herbs extract concentrations were increased and had subtly changing chroma. But, because their K/S values were under 0.5, they were not properly dyed. Color fastness differed according to the natural dyes and herbs' complex dyeing order. The Color fastness was good when the fabrics were dyed with the colorant first, and then with the herb. When the fabrics were dyed with mixture dyeing solution, the natural dye and the herb extract, the herb didn't affect the dyeabilities of fabrics as much. When the fabrics were dyed with the mixture of the colorant and the herb extract, the dyeabilities didn't have much changes. However, their color fastnesses were improved a little bit due to the complex dyeing condition. When the fabrics were dyed with the mixture of the colorant and the herbs extract, their antibacterial activities and deodorization were increased.

• 한국식품저장유통학회지
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• 제18권6호
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• pp.916-922
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• 2011

본 연구는 다양한 생약재를 사용하여 생약재 복합물을 구성하고, 복합물의 이화학적 특성과 항산화 활성에 대하여 조사하였다. 생약재 복합물의 추출 수율은 물 추출물이 35.00%, 에탄올 추출물 25.33%이었으며, 분획물 중에서는 ethyl acetate층의 수율이 물 및 80% 에탄올 추출물에서 각각 0.64% 및 3.76%였다. 생약재 복합물의 물 및 80% 에탄올 추출물의 총플라보노이드 함량은 각각 2.34% 및 2.42%이었고 총페놀성 화합물 함량은 5.04% 및 4.56%이었으며, 용매별 분획물 중 ethyl acetate층의 총플라보노이드 함량이 가장 높았고 총페놀성 화합물 함량 또한 ethyl acetate층에서 가장 높았다. 추출물에 가장 많이 함유된 성분은 salicylic acid와 ${\rho}$-coumaric acid이었다. 물 추출물과 80% 에탄올 추출물의 전자공여능은 각각 43.32% 및 41.32%이었으며, 각각의 용매 분획물의 전자공여능은 총페놀성 화합물 함량의 패턴과 유사하였다. 아질산염 소거능은 물 및 80% 에탄올 추출물에서 9.68% 및 8.94%였으며, 용매별 분획물의 아질산염 소거능은 전자공여능과 유사하였다.

• Natural Product Sciences
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• 제21권2호
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• pp.98-103
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• 2015

Pueraiae Radix (PR), Pueratia Folium (PF) and Sorbus commixta (SC) mixture, namely GS-SP (PR (1)/PF (2)/SC (0.5): v/v/v) was developed as hangover-relieving elixir and its effects on alcoholic metabolism have been investigated. The enzymatic activity of alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) of GS-SP was shown higher than those of single treatment with PR, PL, SC, and the positive control group (YM-808). The survival rate of mouse liver cell line NCTC clone 1469 in the presence of acetaldehyde was 30.6, 22.2, and 8.7% at the GS-SP dosage level of 50, 100, and 200 μg/mL respectively. Different concentrations of 50, 100 and 200 mg/kg of GS-SP showed efficient activity for ADH and ALDH than YM-808 in rat fed with 25% ethanol. The levels of blood alcohol and acetaldehyde after oral administration of 200 mg/kg of GS-SP showed efficient activity of 11.7% and 37% than those of YM-808. These results have been supported to the potential for GS-SP to serve as an excellent potential in providing hangover relief and liver protection.

• 동의생리병리학회지
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• 제19권1호
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• pp.159-166
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• 2005

We observed the efficacy of natural herbs and mixture in treating atopic dermatitis using anti-human IgE treated Human HMC-I cell and NCNga mice model. First, we selected three herbs, Cynonchum witfordii, Diospyros kaki, Ilite which were used to treat skin disease in Traditional Korea Medicine. Using Human HMC-I cell treated with anti-human IgE, we investigate in vitro whether each herb effects on IL-4, IL-13, $TNF-{\alpha}$ expression and $TNF-{\alpha}$, Histamine secretion value. Finally, we conducted study whether the mixture of the selected herbs is more effective than each herb which consist the mixture and control group. The results show that the mixture is better in improving atopic dermatitis condition.

• 대한본초학회지
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• 제32권6호
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• pp.55-62
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• 2017

Objectives : This study aims to evaluate the effects of natural herb mixutre (NHM) on atopic dermatitis and skin regeneration using in vivo test. Methods : NHM was prepared with DW. 25% of NHM was applied to skin lesion, where atopic dermatitis was induced by DNCB in NC/Nga mice. The levels of cytokines (IL-4, IL-5, IL-6, IL-13, TNF-a, and $IFN-{\gamma}$), and IgE in serum were measured by Luminex. Immune cells (WBC, eosinophil, lymphocyte, and monocyte) in blood were counted by coulter counter. The gross investigation of atopic dermatitis index score test were performed during the NHM treatment period. Also, the histopathological change of dorsal skin was observed by H&E and M&T staining. Results : NHM showed the levels of IL-4, IL-5, IL-6, IL-13, IgE, WBC, eosinophil, lymphocyte, and monocyte in serum or blood were significantly decreased. On the contrary, the productions of FGF, and VEGF were increased in the serum. Also, atopic dermatitis index score in NHM-treated mice were observed in the similar levels to those of normal group. Histological examination demonstrated that NHM suppressed immune cell infiltration and thickening of epidermis, meanwhile the extraction induced collagen production in the dorsal skin. Conclusion : This study demonstrated that NHM is appeared to be effective on atopic dermatitis and skin regeneration efficacy based on the observations with hematologic, gross, and histologic examinations. Therefore, we suggest that NHM could be effectively used as an external therapeutics against atopic dermatitis and a consequence skin damage.

• Asian-Australasian Journal of Animal Sciences
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• 제26권8호
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• pp.1127-1136
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• 2013

Four rumen-fistulated crossbred beef cattle (Brahman native) were randomly assigned according to a $4{\times}4$ Latin square design experiment to be fed plant herb supplements in their concentrate mixture. The treatments were: without herb supplementation (Control), lemongrass meal supplementation at 100 g/d (L), lemongrass meal supplementation at 100 g/d plus peppermint powder at 10 g/d (LP), and lemongrass meal supplementation at 100 g/d plus peppermint powder at 10 g/d with garlic powder 40 g/d (LPG), respectively. Based on the present study, the DMI and apparent digestibility of DM, OM, aNDF and ADF were not affected by dietary herb supplementation while CP digestibility tended to be decreased by herb supplement. Moreover, $NH_3$-N and BUN were decreased in all herb supplemented treatments and there was a tendency to an increase in ruminal pH in all herb supplemented groups. While there was no change in TVFA and C4 among lemongrass treatments, C2 was decreased in all herb supplemented treatments while C3 was increased. Methane production by calculation was the lowest in the LP and LPG groups. Population sizes of bacteria and protozoa were decreased in all herb supplemented groups, but not fungal zoospores. In all supplemented groups, total viable and proteolytic bacteria were decreased, while amylolytic and cellulolytic bacteria were similar. More importantly, in all herb supplemented groups, there were higher N balances, while there was no difference among treatments on purine derivative (PD) excretion or microbial N. Based on the results above, it could be concluded that there was no negative effect on ruminal fermentation characteristics and nutrient utilization by plant herb supplement, but protozoal population and $CH_4$ production were reduced. Thus, lemongrass alone or in combination with peppermint and garlic powder could be used as feed additives to improve rumen fermentation efficiency.

• 한국임상보건과학회지
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• 제1권1호
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• pp.83-89
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• 2013

Purpose. Natural herb has been used for traditional medicine for thousands of years. For this reason, alternative medicine methods affect to domestic animal field. The main purpose of this study was to confirm the regulated proteins after feed additive treatment. Methods. We used total thirty male pigs were used for this experiment. E. coli and Salmonella typhimurium, were used for promote enteritis. Animals were divided into a negative control group, positive control group and test group to determine the effect of an additive mixture on the changes in protein expression in serum. The pigs were given a food supplemented with a natural herbal additive containing immunoglobulin yolksac (IgY) at concentrations 1%. On the 1st week and after eight weeks of feeding, the serums were collected from each group. The changes in the serum proteome as a response to the herbal additive were examined using two-dimensional polyacrylamide gel electrophoresis. Results. A total of 14 differentially expressed protein spots were identified by comparing the protein profiles of the control and additive treated porcine serums. Finally, 7 proteins were detected by MALDI-TOF/MS. Moreover, the proteins detected are involved in a cholesterol control factor, inflammation regulator, Conclusion. These results support of the hypothesis that a natural herbal additive containing IgY can affect the immune regulation system and reduce the pathological process by microbial infections.

• 대한임상검사과학회지
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• 제49권3호
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• pp.256-262
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• 2017

식물로부터 유래되는 휘발성 유기 화합물인 에센셜 오일은 인체에 약리효과를 나타낸다고 알려져 있다. 본 연구에서는 화농성 질환과 병독성 질환 비뇨기계통 감염에 나올 수 있는 병원성 세균 Staphylococcus aureus (그람양성알균), Escherichia coli (그람음성막대균), Pseudomonas aeruginosa (그람음성막대균, 극성단모균)을 대상으로 천연 허브 오일 중에 비교적 많이 알려져 있는 라벤더(lavender), 티트리(tea tree), 로즈마리(rosemary), 멜리사(melisa) 4종류의 단일 오일과 비교해, 티트리와 로즈마리, 멜리사를 블렌딩하였을 때 항균력이 상승되는 효과가 있는지 연구하였다. 그 결과 디스크 확산법에서 블렌딩 오일(티트리, 로즈마리, 멜리사)의 경우 단독의 허브오일보다 S. aureus에서18 mm, E. coli 에서 22 mm 와 P. aeruginosa는13 mm의 우수한 항균능력을 확인할 수 있었다. 뿐만 아니라 블렌딩 오일의 경우 최소억제농도(Minimum Inhibitory Concentrations, MIC) 분석에서 S. aureus, E. coli 에서는 0.3125%에서, P. aeruginosa는 1.25%에서 성장억제효과가 확인되었다. 따라서 본 실험에서는 블렌딩 오일이 Oxacillin과 로즈마리, 티트리보다 항균효과가 우수한 것으로 증명되어 단독의 허브오일보다 블렌딩 오일이 우수한 항균력을 나타나는 것을 확인할 수 있었다. 그러므로 허브의 블렌딩을 활용하면 다제 내성균에 대한 항균시너지효과를 발휘할 수 있음을 입증했을 뿐만 아니라 향후 천연보존제와 건강기능성 식품 및 화장품에 활용 가능성을 기대해본다.

• Archives of Pharmacal Research
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• 제21권6호
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• pp.734-737
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• 1998

The hexane fractions from methanolic extracts of Anetheum graveolens L. (Umbelliferae) and Acorus gramineus Soland. (Araceae) revealed potent inhibitory activities against the resistance of multi-drug resistant Staphylococcus aureus SA2 when combined with ampicillin (Am) or chloramphenicol (Cm). As active principles, carvone and the liquid mixture containing carvone from Anetheum graveolens L. and a liquid mixture mainly consisting of benzoic acid phenyl-methyl ester (benzyl benzoate) from Acorus gramineus Soland. were identified. They showed resistance inhibition at the level of 20-50${\mu}g$/ml when combined with 100 or ${\mu}g$/ml of Am or Cm, respectively.

• 혜화의학회지
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• 제13권2호
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• pp.123-129
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• 2004

We observed the efficacy of natural herbs and mixture in treating atopic dermatitis using anti-human IgE treated Human HMC-I cell model. We selected three herbs, Cynonchum witfordii, Diospyros kaki, Ilite which were used to treat skin disease in Traditional Korea Medicine. Using Human HMC-I cell treated with anti-human IgE, we investigate in vitro whether each herb effects on IL-4, IL-13, TNF-a expression and TNF-a, Histamine secretion value. The results show the possibility that the mixture of three herbs may be better in improving atopic dermatitis condition.