A metagenome is a unique resource to search for novel microbial enzymes from the unculturable microorganisms in soil. A forest soil metagenomic library using a fosmid and soil microbial DNA from Gwangneung forest, Korea, was constructed in Escherichia coli and screened to select lipolytic genes. A total of seven unique lipolytic clones were selected by screening of the 31,000-member forest soil metagenome library based on tributyrin hydrolysis. The ORFs for lipolytic activity were subcloned in a high copy number plasmid by screening the secondary shortgun libraries from the seven clones. Since the lipolytic enzymes were well secreted in E. coli into the culture broth, the lipolytic activity of the subclones was confirmed by the hydrolysis of p-nitrophenyl butyrate using culture supernatant. Deduced amino acid sequence analysis of the identified ORFs for lipolytic activity revealed that 4 genes encode hormone-sensitive lipase (HSL) in lipase family IV. Phylogenetic analysis indicated that 4 proteins were clustered with HSL in the database and other metagenomic HSLs. The other 2 genes and 1 gene encode non-heme peroxidase-like enzymes of lipase family V and a GDSL family esterase/lipase in family II, respectively. The gene for the GDSL enzyme is the first description of the enzyme from metagenomic screening.
This study attempt to evaluate the recreation space(two patterns ; one is open space ; forest, water-front space and free open space, the other recreational fercilities) located in the natural area based on clarifying the relationship between the physical conditions of these spaces and human response(users' cognitive evaluation and users' activity pattern). On this standpoint it was proceeded to analyses of the information which was collected by interviews to users who were in this open space at the natural park of Osaka Prefecture. Through this study, the results were summarized as follows ; 1) Forest and waterfront space are considered to be a basic factor of the composition in natural recreation areas. There was difference on the recreational value depending on condition of forest composition. The hardwood-forest apparently high in its efficiency. 2) Free open space is a definite recreational space surely wide in its scope of active of recreational use. The site should be setted up considering the plants conditions around and geographical features according to the recreational activities, and the ground cover should be well controlled. 3) The recreational facilities in natural settings such as the sightseeing tower, the insect display hall, and the camp site appeared to produce low value as a recreational space. It was desirable that recreational activities be allowed within the scope. Consequently, we should carefully consider environmental capacity and landscape to designing these spaces 4) Traditional history and cultural properties are recognized as part of recreational resource as and also as essence of the compositions. So continuos care and proportion of history and cultural properties should be guaranteed.
Journal of the Korean Institute of Landscape Architecture
/
v.35
no.6
/
pp.48-63
/
2008
This research is to apply suitable natural ecosystem evaluation criteria in order to develop the ecosystem conservation, restoration and ways to build substitute habitats as a compensation plan for damaged soundly natural ecosystems in small-scale projects such as resource recovery facility, filtration, etc. The environmental ecology evaluation i.e. generally based on their actual vegetation, community structure, wildlife, water system survey were measured the primary plans for reflecting unique natural environment level of site. As a result, it is necessary to conserve the land in fallow type of wetland, good conservative condition of deciduous forest, wetlanded watercourse for amphibia and reptiles crossing. However, the plan of filtration plant was destroyed wetland(sound ecosystem), natural forest, asian toad spawning area. According to the result of it schemed to build alternative wetland and spawning area, plan to healthy ecosystem and surface soil transplantation as compensation plan. The alternative wetland and spawning area are not only created a various water levels like depth of water is $0{\sim}30cm,\;30{\sim}60cm$, more than 1.5m but also it leads to asian toad spawning and wildlife inhabitant. Moreover, the ecosystem and surface soil transplantation be applied to use the Quercus acutissima forest resources(114 upper trees, 71 canopy trees, 401 shrubs) and surface soil$(5,072m^3)$ in ecology creation sets.
Journal of the Korean Institute of Landscape Architecture
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v.34
no.4
s.117
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pp.37-47
/
2006
The city of Siheung in the Kyunggi-do Province has various natural resources such as the ocean, mountains, wide farmland, various types of wetlands, streams and rivers. In addition, the city has a big greenbelt, which consists of two-thirds of the area, where development has been regulated. Since the city has a relatively well-preserved natural environment, it offers a great potential for regional development. The purpose of this study is to create an eco-tourism resource plan for the Mulwang reservoir, which offers many opportunities for ecotourism in the city of Siheung. This study includes a literature review for planning elements and suggests a comprehensive plan that includes conservation, eco-restoration, route program and practice program for ecotourism in and around the site. The plan also includes eco-farming, a visitor center, an ecovillage, the chance to experience livestock farming, opportunities to learn about and experience the forest, tracking, eco-learning, an environmental interpretation facility, fishing and aquatic-oriented leisure activities. This study's process and results show possibilities that can be applied to other areas where eco-tourism using natural resources is used for regional development.
This study is focused to the national park of Korean typical Sea Hally$\check{o}$ Haesang, and its visual resources and practiced inspect course by the way of suppositions and tests, to show the visual resource management objectively, and that of qualitative basic data. Accordingly by measuring the physical amount spatial structure with the visual amount originated from the Mesh Analyzing Method and the Visual Preference from the Scenic Beauty Estimation(S.B.E.) method and analyzed the valuation of the visual resource by Iverson method. Spatial image structure measured by Semantic Differential(S.D.) Scale was shown through the factor analysis algorithm for the analyzing psychological amount and examined the flowing out of decisive factor and the objective importance related to the mutual factors by appling the measurement of the visual quality. As a national Park, the visual factors that have natural landscape harmonized with forest, sky, surface of the water, curious stones and rocks, and temples should be escalated their values affirmatively so as to be the scenery of pointed direction and enjoyable, and it is of more needed for visual resource and its' controlling technique to make artificial structures more intentional planning and systemical setting. When we are viewing the improvement for the national park along with the visual resource management, reasonable level of development is needed, because when men interference surpass plantations and leasts will be damaged and the quality of natural landscape can be lowered, so it is needed to set up a management end, tangibly or clearly; and it is permitted limit coming and going ablably by accounting the suitable number for availing. But the controling end should be set in every level, positive management, very actively within the permissive varcability. It is the main business for the national park to prevent the damage from human for their gay life or to prevent the damage of a land carpet, and to restorate for the visual resource management.
A new methodology to detect forest fire burnt scars at near real time using MODIS (Moderate-resolution Imaging Spectroradiometer) data is presented here with a goal of introducing a new and improved capability to detect forest fire burnt scars in Thailand. This new technology is expected to increase the efficiency and effectiveness of the forest fire tackling resources distribution and management of the country. Using MODIS data in burnt scars detection has two major advantages - high availability of data and high resolution per performance ratio. Results prove the near real time algorithm suitable and working well in order to monitor the forest fire dynamic movement. The algorithm is based on the threshold separated linear equation of burnt and un-burnt. A ground truth experiment confirms the burnt and un-burnt? areas characteristics (temperature and NDVI). A threshold line on a scatter plot of Band I and Band 2 is determined to separate the burnt from un-burnt pixels. The different threshold values of NDVI and temperature use to identify pixels' anomaly, abnormal low NDVI and high temperature. The overlay (superimpose) method is used to verify burnt pixels. Since forest fire is a dynamic phenomenon, MODIS burnt scars information is suiting well to fill in the missing temporal information of LANDSAT for the forest fire control managing strategy in Thailand. This study was conducted in the Huai-Kha-Kaeng (HKK) Wildlife Sanctuary, Thailand
Kim, Hyun-Ji;Jeong, Hye-Ran;Ku, Ja-Jung;Choi, Kyung;Park, Kwang-Woo;Cho, Do-Soon
Korean Journal of Environmental Biology
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v.30
no.2
/
pp.90-97
/
2012
Environmental characteristics and vegetation of the natural habitats of Eranthis byunsanensis B.Y. Sun were investigated in order to provide the basic data for conservation, restoration, and utilization of this Korean endemic plant. This study was conducted in Anyang, Byeonsan, Geoje, Gyeongju, Jeju, Ulsan and Yeosu. E. byunsanensis was distributed around the altitudes of 84~585 m with a slope degree of $10{\sim}20^{\circ}$, and mostly formed discontinuous populations in north-east part of valleys. Soil analysis showed the mean organic matter of 9.6% and a slightly acidic pH (mean pH of 4.9). The mean gravimetric water content was 16.5%. Correlation coefficients between environmental factors and community characteristics suggested that there was a positive correlation between slope degree and soil water content, between slope degree and soil pH, between soil organic matter and importance value, and between species richness or evenness and species diversity. The vascular plants from 59 quadrats of 7 habitats were identified into 144 taxa. A few species were dominants and similarly distributed in Byeonsan, Jeju, Ulsan and Yeosu. The highest species diversity was found in Geoje (1.43), while Anyang showed the lowest (0.87). Species evenness of Gyeongju and Jeju was bigger than 0.8, but that of Geoje was the lowest (0.59). Dominant species of woody plants in and around the 59 plots were represented by high frequency of Acer pictum subsp. mono, Carpinus cordata, Lindera obtusiloba, and Carpinus laxiflora. The results of this study can provide useful data for conservation and restoration of natural habitats of Korean endemic Eranthis byunsanensis and for the development and growth of this species for ornamental purposes.
Journal of the Korean Institute of Landscape Architecture
/
v.17
no.1
/
pp.1-16
/
1989
^x As mentioned above, I consider the change of role that is related with Japanese thought of Natural Environmental Conservation, and I divide each part of periods into forest, farmland, open space, urban planning, environmental conservation etc. To summary the content of each period of Natural Environmental Conservation thought is as follows. 1) Previous period before formation(before 1919) In the previous period of Meiji, it could be said that the consciousness of the Natural Environmental Conservation was coming to existence. In this, so called, Natural Environmental Conservation, the thought of Forest Preservation was the main current and it was the thought of territorial integrity for the security of resource, the forestry conservancy and flood control in the forestry farmland. Since the age of Meiji, the theory of Natural Conservation appears from the theory of Japanese landscaping and systematic management about public parks was enforced, but the regular theory of Natural Environmental Conservation did not attain full growth. 2) The period of formation(1919~ 1954) In the period of formation, the base of Natural Environmental Conservation had been established. Parks and Open spaces were admitted as a public facilities in each city, and legislations which supported it had been continuously enacted and so on. In this period, the afforestation counter plan was emphasized on the side of territorial integrity. In the mountainous district. tree planting was emphasized to recover the forest which had been destroied by war. 3) The period of development(1955~ 1974) In the period of development, varied policies was institutionalized for the Natural Environmental Conservation. However, all sorts of development policy had performed simultaneously, thus development had complicated relation with preservation. But after 1970's the framework of system of Natural Environmental Conservation improvement was constituted to control the many kinds of development, and the spontaneous campaign of nature preservation by private lead was being taken root. This is the more progressive period. 4) The pried of root(after 1975) After the latter of 1970's, the role of Natural Environmental Conservation began to take root not in the direction of control but inducement, and, getting out of development - oriented policy, aimed at relation between human being and natural environment and the side of amenity of human environment. Besides, the current of Natural Environmental Conservation had been seperately progressed in the cities and forests. Since this period, it was the character that cities and forests began to be unified as 'amenity'space of man.
The purpose of this study was to identify the effects of expectancy-disconfirmation (ED) of attraction affecting visitor's satisfaction in natural recreation forest (NRF). After reviewing the literature about recreational resources (RRs), facilities, ED, and visitor's satisfaction in NRF, this study analogized 11 attractions of NRF and constructed the conceptual framework. This study had obtained data through a questionnaire, which surveyed 415 visitors at 3 NRFs in 204 summer season. This study found that 1) The expectancy-performance of visitor's interesting resources is the highest among RRs, 2) There was mean difference between expectancy and performance of attraction at one percent significant level, 3) Safety facility had the highest ED value and plant resources had the lowest ED value, 4) The higher ED value of attractions, the lower value of visitor's satisfaction, 5) in multivariate analysis, such variables as physical resource, social culture resource, lodging facility, foundation facility, safety facility, sanitary facility, and education facility have been turned out be statistically significant at one percent level, 6) The relative contribution of the ED of foundation facility, lodging facility, and education facility on visitor's satisfaction have ben determined to have respectively 2.50, 1.48, and 1.88 times more important than that of physical resources.
Byeon, Jun Gi;Shin, Jae Kwon;Jung, Su Young;Kim, Dong-Kap
Korean Journal of Plant Resources
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v.30
no.2
/
pp.219-239
/
2017
This study was carried out to investigate the vascular plants of the protected area for forest genetic resource conservation in the National Yonghyeon natural recreation forest. The vascular plants collected 7 times (from February to September 2014) were consist of total 460 taxa; 95 families, 280 genera, 398 species, 3 subspecies, 50 varieties and 9 forms respectively. The 6 taxa of Korean endemic plants and 8 taxa of Korean rare plants were investigated. The floristic regional indicator plants found in this area were 43 taxa comprising 7 taxa of grade IV, 7 taxa of grade III, 12 taxa of grade II, 17 taxa of grade I. The naturalized plants were 39 taxa, therefore naturalized ratio was 8.5%. 460 taxa listed consist of 189 taxa (41.1%) of edible plants, 155 taxa (33.7%)of medicinal plants, 177 taxa (38.5%) of pasture plants, 52 taxa (11.3%) of ornamental plants, 17 taxa (3.7%)of timber plants, 12 taxa (2.6%) of fiber plants and 3 taxa (0.7%) of industrial plants. The vegetation status of investigated area was comparatively well conserved, but human-induced damage is increasingly greater. Therefore, a long-term monitoring of vascular plants and vegetation movement must become accomplished.
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