• 생약학회지
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• 제29권1호
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• pp.28-34
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• 1998

It has been known that diseases related with aging or cancer result, at least in part, from free radicals, and antioxidants may reduce or prevent the abnormalities associated with free radical formation via its scavenging action. Thus, this study was carried out to investigate the antioxidative and antimicrobial activities of nut species for the purpose of developing antioxidant from natural products. Antioxidant activities of four kinds of nuts such as chestnut, groundnut, walnut and acorn were examined by measuring the radical scavenging effect on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical. The extracts from chestnut hull and acorn barnyard millet showed strong antioxidative activities among 10 samples tested. Because of their highest antioxidative activity among 10 samples, radical scavenging effects of 4 different extract compartments (Hexane, EtOAc, BuOH and $H_2O$ extracts) from chestnut hull and acorn barnyard millet, were further examined by DPPH method. EtOAc and BuOH extracts exhibited antioxidative activities similar to those of natural, tocopherol or synthetic antioxidants, BHA. These findings demonstrates that major fraction of the antioxidative activity of chestnut hull or acorn barnyard millet was the EtOAc and BuOH extract compartments. However, antimicrobial activities against food-related bacteria and yeasts was relatively weak.

• 한국염색가공학회지
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• 제16권2호
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• pp.41-46
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• 2004

We have studied on the experimental variables such as optimum adsorption condition, color changes, light fastness, antimicrobial properties, and wash durability of the cotton fabrics treated with gallnut extracts. Treatment of gallnut extracts on the cotton fabrics was optimally achieved at 6$0^{\circ}C$ for 60min. Gallnut extracts did not cause unintended color change after treatment on the cotton fabrics dyed with reactive dyes. The cotton fabrics treated with gallnut extracts showed good light fastness, though we took into the account the extract is a natural material. The cotton fabrics treated with 10.0% o.w.f. of gallnut extracts had strong antimicrobial activity and good wash durability.

• 한국미생물·생명공학회지
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• 제32권4호
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• pp.352-355
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• 2004

The extracts from 70 different plant natural products were used to investigate the antimicrobial activities against Brachyspira hyodrsenteriae, the causative agent of swine dysentery. Among them, the extracts from Terminalia chehula exhibited the highest antimicrobial activities against B. hyodysenteriae. It showed the antimicrobial activity against B. hyodysensteriae mostly in parts of its shell. Minimum inhibitory concentration (MIC) of the water extracts of T chehula was 12.5 mg/ml. After organic solvent extractions, the ethylacetate extracts exhibited higher antimicrobial activities against B. hyodysenteriae, and their antimicroactivities were similar to that of the tannin. In conclusion, these findings suggest that the extracts from T chebula may be used as animal feed additives for their antimicrobial activities against B. hyodysenteriae.

• Advances in Traditional Medicine
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• 제8권4호
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• pp.400-407
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• 2008

The aim of the present study is to investigate the anti-inflammatory and antimicrobial activity of petroleum ether and ethanol extracts of Scutia myrtina (Family: Rhamnaceae). In anti-inflammatory activity carrageenan and histamine induced paw oedema and cotton pellet induced granuloma for acute and chronic inflammatory models were studied in Wister albino rats. Based on the results of the present study it can be concluded that petroleum ether and ethanol extract of Scutia myrtina at 400 mg/kg has potential anti-inflammatory effect and they act in a dose dependent manner. Both extracts of Scutia myrtina showed antimicrobial activity against all bacterial and fungal strains tested at the concentration of $100\;{\mu}g$/ml. From the result, it can be concluded that the Scutia myrtina contain antibacterial and antifungal principle. Further more, besides the confirmation of the popular use; the obtained results demonstrate this herbal drug to represent a new source of antimicrobial and anti-inflammatory agent.

• Current Research on Agriculture and Life Sciences
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• 제31권3호
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• pp.170-176
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• 2013

In this study, we selected some material to have potential bioactivity from natural plants, confirmed as basic data for industrializing and tried to develope the food materials using them. DPPH, ABTS, antioxidant protection factor, TBARs and antimicrobial activity of extracts from Rosa multiflora Thunberg fruits were determined. The total phenolics extracted from Rosa multiflora were 12.08, 11.82, 11.1 and 12.6 mg/g when using water, 70% ethanol, 70% methanol and 70% acetone as the solvent, respectively. The optimum conditions for extracting the phenolic compounds were 70% ethanol over for 12 hrs(11.82 mg/g). The electron donating ability and inhibition rate on ABTS of the 70% ethanol extracts were 97% and 92.2%, respectively while the antioxidant protection factor(PF) of the water extracts and 70% ethanol extracts were 1.79 and 1.34 PF, respectively. The TBAR (thiobarbituric acid reactive substance) value were $1.3{\mu}M$ for the control and $0.15{\mu}M$ for the 70% ethanol extracts. The inhibitory activity against ${\alpha}$-amylase was 26% for the 70% ethanol extracts. The 70% ethanol extracts from Rosa multiflora Thunberg fruits exhibited antimicrobial activity against H. pylori, S. epidermidis, S. aureusand and E. coli with clear zone diameters of 14, 25, 14 and 13 mm, respectively when using $200{\mu}g/mL$ of the phenolic compounds. An HPLC analysis identified 6 major phenolic metabolites in the Rosa multiflora Thunberg fruits extracts: rosmarinic acid, caffeic acid, chlorogenic acid, courmaric acid, protocatechuic acid and quercetin. In particular, the content of rosmarinic acid was the highest in the 70% ethanol extracts. Therefore these results indicate that 70% ethanol extracts from Rosa multiflora Thunberg fruits can be useful as a natural antioxidant and in functional foods.

• 치위생과학회지
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• 제4권1호
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• pp.45-48
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• 2004

In the current research for natural products with antimicrobial effects, various extracts of Puerariae thunbergiana and isoflavones against microorganisms were evaluated in terms of the minimum inhibitory concentrations (MIC). In general, Candida albicans was stronger antimicrobial activity than the other microorganisms such as Streptococcus mutans, Staphylococcus epidermidis, and Staphylococcus aureus. The maximum activity was exhibited by methanol extract of the leaves of Puerariae thunbergiana Beth. against Candida albicans(MIC, $400{\mu}g/mL$). These results suggest that methanol extract of Puerariae thunbergiana has a potential antimicrobial activity.

• 한국식품영양과학회지
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• 제28권1호
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• pp.100-106
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• 1999

Use of chemical preservative for controlling harmful microorganisms in food products has been debated due to public concerns about food quality because of perceived toxic and carcinogenic potential. Thus, use of non toxic natural antimicrobial agents has become essential. This study was investigated to determine the antimicrobial activity of water or ethanol extract of commercially available tea, and of solvent fractionated ethanol extracts obtained from steamed green tea. Both of water and ethanol extracts of green tea(steamed or roasted), oolong tea and black tea exhibited strong antimicrobial activity against gram positive and negative bacteria, but not effective against yeast and mold. Also, antimicrobial activity of ethanol extract of 4 different kinds of tea was stronger than that of water extract. Among 4 different tea, ethanol extract of steamed green tea was further fractionated. One thousand g/disk buthanol extract had the strongest antimicrobial activity against bacteria and mold. The concentration of the antimicrobial activity of buthanol extract in tested microorganisms ranged from 125~1000 g/disk except for Rhizopus javanicus. Antimicrobial activity of buthanol extract of steamed green tea was not destroyed by heating at 100oC for 60 min and at 121oC for 15 min, which is very stable over heat treatment. The inhibitory effect of the buthanol extract on the growth of Listeria monocytogenes and Staphylococcus aureus was investigated. Growth of both strains was started in the presence of 250 and 500 g/ml after 12 and 24 hour respectively, whereas complete inactivation of both strains was occurred in the presence of 1000 g/ml.

• Food Science and Biotechnology
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• 제15권4호
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• pp.582-588
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• 2006

The antioxidant and antimicrobial activity of the fermentation extract (PFE) and the 50 and 80% ethanol extracts (PE 50, PE 80) of Pinus densiflora pine needles were evaluated. Electron donating ability, superoxide dismutase (SOD) ability, and antimicrobial activity were observed in PFE; those abilities differed in PE 80 and PE 50, depending on the ethanol concentration used for the extraction. PFE had the highest electron donating ability with a value of 92.20%, while PE 80 and PE 50 had values of 74.66 and 53.47%, respectively. For SOD activity, PE 80 exhibited a slightly higher value of 31.11% compared to that of PFE and PE 50, which were 29.65 and 25.43%, respectively. PFE, PE 50, and PE 80 were all found to inhibit bacteria, and the effectiveness of this inhibition was strongly related to the type of extracts used. PFE showed good antimicrobial effects for all of the tested Gram-positive strains and for most of the tested Gram-negative strains. These results suggest that PFE has superior functionality compared to the ethanol extracts (PE 80, PE 50), in terms of antioxidant and antimicrobial activity. On the basis of these results, pine needle fermentation extracts can be used for industrial applications as a functional material.

• 한국응용과학기술학회지
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• 제37권2호
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• pp.279-286
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• 2020

치아우식증 치료물질을 개발하기 위하여 한국에서 전통적으로 널리 사용되어왔던 20종류의 약용식물로부터 추출물을 분리하였다. 이를 이용하여 치아우식증 원인균인 Streptococcus sobrinus KCOM 1157에 대한 항균 활성을 나타내는 약용식물들을 조사하였다. 감초, 고삼, 황련, 박하, 송절, 부평초 등의 추출물들이 S. sobrinus KCOM 1157에 대해서 항균 활성을 보여주었다. 이 중 송절, 고삼, 황련 등의 천연물들은 높은 항균 활성을 나타내었다. 이 결과들은 송절, 고삼, 황련의 천연물들이 S. sobrinus 를 포함한 구강질환 세균들에 대한 항우식증 치료제로서의 가능성이 있음을 의미한다.

• 대한수의학회지
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• 제54권3호
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• pp.159-164
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• 2014

Bacterial infection of the uterus in Hanwoo can kill embryos. Therefore, many antibiotics have been used to treat this infection in the uterus of repeat breeders. Incorrect use of antibiotics has led to resistance in bacteria. Natural compounds have used as substitutes for antibiotics because they are safe and have very mild side effects. This study was conducted to examine the antimicrobial effects of five extracts from medicinal plants including Humulus japonicas (Hj), Phelledendron amurense (Pa), Viola mandshurica (Vm), Carthamus tinctorius (Ct), and Chelidoni herba (Ch) on bacteria isolated from the uterus of Hanwoo using the paper disc diffusion method. Hj and Pa extracts had potent antimicrobial effects against Staphylococcus lentus, Streptococcus infantarius subsp. coli, and Bacillus pumilus. Pa had the greatest antimicrobial effect among the five medicinal plants and was effective against 19 types of bacteria from bovine uterus. Compared to Pa, Hj showed weaker antimicrobial effects on all the bacteria tested except Pseudomonas aeruginosa. Vm, Ct, and Ch also showed weak antimicrobial effects on the tested bacteria. The results obtained suggest that Hj and Pa are natural compounds suitable for treating bacterial infection in repeat breeders and improving conception rates of Hanwoo.