• 한국의류산업학회지
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• 제26권2호
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• pp.190-197
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• 2024

With increasing global interest in environmentally friendly materials and the consequent rise in demand, there is a growing need for alternatives to synthetic fibers, which can cause skin diseases and other side effects. The fashion industry is emphasizing material sustainability owing to concerns about increasing carbon emissions. Moreover, consumers express a strong desire for ecofriendly and sustainable materials. Therefore, clothing brand companies are developing eco-friendly products to enhance their corporate image. Hemp fibers are recognized for their functionality and are utilized as crucial materials in the development of eco-friendly products by global fashion companies. In this study, we produced socks that effectively improve foot health using hemp stem bark extract fibers and demonstrated the positive efficacy of natural fibers through functional and wearability evaluations. Hemp stem bark extract fibers showed 99.9% antimicrobial effectiveness against bacteria responsible for sweat-induced bacterial proliferation and odor, when blended with lyocell fibers and woven into fabric to manufacture socks. Wearability evaluations of these terry cloth socks confirmed a reduction in foot odor and fatigue among the participants with a consumer satisfaction of 4.63/5. These findings confirm the effectiveness and positive impact of the natural antimicrobial properties of hemp fibers and terry cloth structure in improving foot health.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.669-671
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• 2000

Antimicrobial cationic peptides have attracted increasing research and clinical interest as a natural antibiotics due to their broad spectrum of antimicrobial activites and the rapid development of multidrug-resistant pathogenic microorganisms. In this study, first, we synthesized artificial fusion partner and cationic peptide genes (lactoferricin, magainin, protegrin-1, and indolicidin). Second, we constructed recombinant expression vectors and then transformed Pichia pastoris. Finally, expressed cationic peptides were purified and tested for their antimicrobial activites. Antimicrobial activity has been tested upon the appearance of clearing zone on the plate with the lawn of gram negative E.coli XL- I blue and garm positive Staphylococcus aureus. Protegrin-1 and Indolicidin have apparant activity of cationic peotides. This fusion technique may lead to a general and suitable tool for production of pure antimicrobial cationic peptides in Pichia pastoris.

• 한국지역사회생활과학회지
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• 제26권4호
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• pp.697-705
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• 2015

This study investigates antimicrobial effects of food-borne pathogens and the antioxidant activity of Torreya nucifera extract. The growth of food-borne pathogens including Staphylococcus aureus, Pseudomonas aeruginosa, and Candida albicans was inhibited by the extract. The antimicrobial activity of the extract was highest for Staphylococcus aureus among seven gram-positive bacteria and for Pseudomonas aeruginosa among six gram-negative bacteria. The extract exhibited slightly lower DPPH radical-scavenging activity, but its ABTS radical-scavenging activity was higher than that of ${\alpha}$-tocopherol. The results demonstrate the extract's antimicrobial effects on food-borne pathogens as well as potent antioxidant capacity and suggest that Torreya nucifera may be used as a natural antibacterial agent and an effective antioxidant in food.

• 한국자원식물학회지
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• 제24권6호
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• pp.719-723
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• 2011

Antimicrobial activities of methanol, ethanol, water, and $CH_2Cl_2$ extracts from Fritillaria unibracteata Hsiao et K.C. Hsia and F. ussuriensis Maxim. were investigated by disk-agar diffusion method. The result showed comparatively strong antimicrobial activity against several microorganisms. The extracts from F. unibracteata and F. ussuriensis dosedependently increased the activity. However, water and $CH_2Cl_2$ extracts showed no antimicrobial activity against 7 microorganisms. Especially, against the most sensitive microorganism Staphylococcus epidermidis, methanol extracts at highest concentration of 20 mg/mL exhibited the largest clear zone on plate by 6-12 mm and ethanol extracts on plate by 6-10 mm.

• Journal of Oral Medicine and Pain
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• 제25권2호
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• pp.153-158
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• 2000

This experiment was performed to investigate effects of boiled-water extract of artemisia on the important oral microflora, Staphylococcus aureus, Streptococcus mutans, and Candida albicans, and to examine the difference of antimicrobial effects according to concentration of extract. The bacteria was cultured in broth media containing 0.1%, 0.5%, 1.0% of artemisia extract, and sterile distilled water respectively. After harvesting the culture, the genomic DNA of each aliquot was extracted and DNA concentration was relatively compared by means of agarose gel electrophoresis. As a result, we found out that the boiled-water extract of artemisia had significant antimicrobial effects on Staphylococcus aureus, Streptococcus mutans, and Candida albicans and its antimicrobial effects was increased in proportion to its concentration.

• 한국응용과학기술학회지
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• 제37권2호
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• pp.279-286
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• 2020

치아우식증 치료물질을 개발하기 위하여 한국에서 전통적으로 널리 사용되어왔던 20종류의 약용식물로부터 추출물을 분리하였다. 이를 이용하여 치아우식증 원인균인 Streptococcus sobrinus KCOM 1157에 대한 항균 활성을 나타내는 약용식물들을 조사하였다. 감초, 고삼, 황련, 박하, 송절, 부평초 등의 추출물들이 S. sobrinus KCOM 1157에 대해서 항균 활성을 보여주었다. 이 중 송절, 고삼, 황련 등의 천연물들은 높은 항균 활성을 나타내었다. 이 결과들은 송절, 고삼, 황련의 천연물들이 S. sobrinus 를 포함한 구강질환 세균들에 대한 항우식증 치료제로서의 가능성이 있음을 의미한다.

• Journal of Microbiology and Biotechnology
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• 제22권10호
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• pp.1367-1374
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• 2012

Pleurocidin, a 25-mer antimicrobial peptide, has been known to exhibit potent antibacterial activity. To investigate the functional roles in N- and C-terminal regions of pleurocidin on the antibacterial activity, we designed four truncated analogs. The antibacterial susceptibility testing showed that pleurocidin and its analogs exerted antibacterial effect against various bacterial strains and further possessed specific activity patterns corresponding with their hydrophobic scale [pleurocidin > Anal 3 (1-22) > Anal 1 (4-25) > Anal 4 (1-19) > Anal 2 (7-25)]. Fluorescence experiments using 1,6-diphenyl-1,3,5-hexatriene (DPH) and 3,3'-dipropylthiadicarbocyanine iodide [$diSC_3(5)$] indicated that the differences in antibacterial activity of the peptides were caused by its membrane-active mechanisms including membrane disruption and depolarization. Blue shift in tryptophan fluorescence demonstrated that the decrease in net hydrophobicity attenuates the binding affinity of pleurocidin to interact with plasma membrane. Therefore, the present study suggests that hydrophobicity in the N- and C-terminal regions of pleurocidin plays a key role in its antibacterial activity.

• 한국염색가공학회지
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• 제24권4호
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• pp.247-252
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• 2012

The objective of this study was to find an appropriate method and process for protecting the green color in bamboo leaves and subsequent extraction of the green colorants, chlorophyll. Various inorganic salts including cupric sulfate, ferric sulfate, and zinc chloride were employed as green color protectors. Accordingly, the effects of metal salts and treatment conditions on color protection were investigated to find appropriate protector and conditions. And also, the efficacy of bamboo colorants as a natural green dye was evaluated through dyeing and colorfastness tests. Antimicrobial activity of dyed fabrics was measured by shake flask method in terms of bacterial reduction rate using Staphylococcus aureus(ATCC 6538). On the basis of experimental results for stabilizing bamboo leaves colorants, it was confirmed that Cu was the most appropriate metal type considering dye uptake, photostability and light fastness, and its optimum concentration was 0.025%. After the stabilization, the colorants were extracted efficiently at NaOH aqueous solution of 1.00%. It was concluded that bamboo leave has a high potentiality as new resources to produce a natural green dye with antimicrobial functionality.

• 한국동물위생학회지
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• 제34권2호
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• pp.167-178
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• 2011

Garlic (Allium sativum L.) has long history of reputed value and actual use for its medicinal, antimicrobial and pesticidal properties. This study was conducted to find possible developments to natural food preservatives and natural antimicrobials from garlic extracts. The antimicrobial activities of raw garlic extract, heat, pH, temperature against pathogenic bacteria were investigated. E. coli, S. Enteritidis, L. monocytogenes, and S. aureus exhibited antimicrobial activities at 20% garlic extract, but no antimicrobial activity was seen in E. faecium. Raw garlic extract and garlic extract heated for 2 min at $95^{\circ}C$ showed strong antimicrobial activities, but the antimicrobial activity of garlic extract heated for 10 min at $95^{\circ}C$ was much less. The antimicrobial activities of 50% garlic extract adjusted pH 4.0~7.0 showed much the same, but the antimicrobial activities decreased at pH 8.0 or higher. The antimicrobial activities by storage $-18^{\circ}C$ of garlic extract showed much the same. When five strains were cultured for 72 hr at $35^{\circ}C$ in the TSB containing 1~10% garlic extract, viable cell number of five strains were decreased to $10^0{\sim}10^4$ CFU/ml even at 1% or 2.5% (E. faecium) after 24 hr, but later increased to $10^4{\sim}10^9$ CFU/ml after 72 hr. When five strains were cultured for 21 day at $4^{\circ}C$ in the TSB containing 1%, 2.5%, 5%, and 10% garlic extract, viable cell number of E. coli, S. Enteritidis, and S. aureus were decreased to $10^3$, $10^0{\sim}10^2$, $10^1{\sim}10^4$ CFU/ml after 21 day, respectively, but L. monocytogenes and E. faecium increased to $10^8$ and $10^6$ CFU/ml after 21 day.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2018년도 추계학술대회
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• pp.91-91
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• 2018

The Rosa multiflora, a well-known plant belonging to Rosacea, is widely used in orthodox medicine in worldwide. However, its biological activity as a functional ingredient for cosmetic products have not yet been studied. Accordingly, an investigation of the above mentioned atrributes was performed on a 50% ethanol extract of Rosa multiflora. The antioxidant activities were determined by DPPH. Additionally, the contents of total phenols and flavonoids were analyzed. Also, the phenolic compounds were detected using HPLC. The melanogenesis regulatory effect was evaluated using melanin content and cellular tyrosinase activity in B16F10 melanoma cells. The elastase inhibitory activity assay was performed for anti-wrinkle effect. The antimicrobial activity was assessed using the disc diffusion assay. The DPPH radical scavenging ability, denoted by the $SC_{50}$ value was found to be $123.1{\mu}g/ml$, whereas that of positive control (ascorbic acid) was $27.5{\mu}g/mL$. The content of total polyphenol and flavonoid content were 202 mg/g and 86.77 mg/g, respectively. In addition, astragalin and gallic acid were identified in the extract. Also, the ethanol extract significantly inhibited ${\alpha}$-MSH-induced melanogenesis in B16F10 cells. For anti-wrinkle effect, elastase inhibition activity of the ethanol extract was 53.2% at a concentration of $100{\mu}g/ml$. The antimicrobial activity of the extract against S. aureus and E. coli was observed to be 0.5 - 5%, and no significant activity was noted against C. albicans. Therefore, the ethanol extract of Rosa multiflora can be used effectively for development of functional cosmetic materials.