• Title/Summary/Keyword: native soybean

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Basic Studies on the Native Colored-Soybean Cultivars II. Classification of Collected Soybean Varieties by the Multivariate Analysis (유색 대두수집종의 특성 연구 제II보 밭밑콩 수집유색재래종의 다변량에 의한 품종분류)

  • 구자옥;이영만;신동영
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.28 no.3
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    • pp.340-344
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    • 1983
  • Taxonomic distances and Q correlations of all possible comparisons among thirty-two collected soybean varieties were calculated from the standardized mean of twenty-one characters. Ten varietal groups were classified by the single linkage clustering based on Q correlations. The means of Q correlations of intra-group were higher than those of inter-group. Each groups were characteristic in each mean of characters within varietal groups.

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Expression and purification of Soybean $\beta$-Conglycinin from ($\beta$-Conglycinin의 대장균 발현과 정제)

  • 노영희
    • The Korean Journal of Food And Nutrition
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    • v.12 no.2
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    • pp.184-190
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    • 1999
  • Soybean protein consists of two major components $\beta$-conglycinin and glycinin which together consti-tute 70% of the total seed storage protein at maturity. $\beta$-Conglycinin is trimeric glycoprotein and for-med by the assembly of various combinations of three subunits $\alpha$,$\alpha$' and $\beta$ which have molecular weig-hts of 69,000, 72,000 and 42,000, respectively. Recently $\beta$-conglycinin was identified as powerful LDL lip-oprotein receptor activation hypercholesterolemia and major allergenic proteins. To investigate these reasons we constructed an expression system of cDNA encoding $\alpha$-subunit of $\beta$-conglycinin in Escherichia coli and purified the expressed protein. The pro-$\beta$-conglycinin synthesized in Escherichia coli BL 21 (DE3)comprised approximately 15% of the total bacterial proteins and the expressed protein are formed sol-uble and trimer such as native protein in Escherichia coli cells. The highly expressed protein was purified to homogeneity by salt precipitation with 20~40 % ammonium sulfate ion-exchange chromatography with Q-sepharose and hydrophobic column chromatography with Butyltoyopearl.

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Role of Fermentation in Improving Nutritional Quality of Soybean Meal - A Review

  • Mukherjee, Runni;Chakraborty, Runu;Dutta, Abhishek
    • Asian-Australasian Journal of Animal Sciences
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    • v.29 no.11
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    • pp.1523-1529
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    • 2016
  • Soybean meal (SBM), a commonly used protein source for animal feed, contains anti-nutritional factors such as trypsin inhibitor, phytate, oligosaccharides among others, which limit its utilization. Microbial fermentation using bacteria or fungi has the capability to improve nutritional value of SBM by altering the native composition. Both submerged and solid state fermentation processes can be used for this purpose. Bacterial and fungal fermentations result in degradation of various anti-nutritional factors, an increase in amount of small-sized peptides and improved content of both essential and non-essential amino acids. However, the resulting fermented products vary in levels of nutritional components as the two species used for fermentation differ in their metabolic activities. Compared to SBM, feeding non-ruminants with fermented SBM has several beneficial effects including increased average daily gain, improved growth performance, better protein digestibility, decreased immunological reactivity and undesirable morphological changes like absence of granulated pinocytotic vacuoles.

Study on Native Local Foods in Andong Region (안동(安東)지역의 향토음식에 관한 고찰)

  • Yoon, Sook-Kyung
    • Journal of the Korean Society of Food Culture
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    • v.9 no.1
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    • pp.61-69
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    • 1994
  • Northern Kyungbuk around Andong is a mountainous and more or less dry region geomorphogically. Since this area located in the valley was less invaded by foreign countries, the folk traditions have been maintained for a long time. Also as the center of Confucian culture, this region respects ceremonies and so, the simple and noble ceremonial-foods such as Ddok, Hankwas, and Buchiki have been firmly preserved in many distinguished families. And besides Andong-Soju, many traditional liquors produced in different methods can be found in this region. As Gogi-Sikhae(a fermented beverage utilizing rice and fish) is still preserved in eastern coastal area, Sosikhae(Sikhae without fish) is maintained in this region and furthermore the unique Andong Sikhae developed by modifying Sosikhae is being consumed currently. Since it is difficult to get fish here geographically, the soybean foods were developed for protein intake, for example, various fermented soybean pastes, Andong-Songuksu(a hand-made noodle using raw soybean flour), and Siragiguk(a vegetable soup).

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Investigation of useful components in soybean seeds: Purification and characterization of soybean ferritin (콩 유용성분 탐색에 관한 연구: 콩 Ferritin의 정제 및 특성)

  • Seo, Kyung-Won;Oh, Suk-Heung
    • Applied Biological Chemistry
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    • v.41 no.7
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    • pp.522-526
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    • 1998
  • Ferritin from germinated soybean seeds was purified by ammonium sulfate precipitation (0.55 saturation), ion-exchange chromatography on DEAE-cellulose, gel filtration chromatography on Sephacryl S-300, and HPLC with Bio-Scale Q2 column. SDS-PAGE analysis showed that the purified ferritin is composed of subunit with an apparent M, 21,000. The molecular mass of the native soybean ferritin estimated by gel filtration on Sephacryl S-300 and non-denaturing polyacrylamide gel electrophoresis appeared to be $510{\sim}560\;kDa$. Soybean ferritin contained 833 mol Fe/mol protein, which is 31-fold more iron than pumpkin ferritin and stained positive for iron on non-denaturing gel. Soybean ferritin cross-reacted with anti-soybean rabbit ferritin antiserum.

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Strain Recognition and Classification of Korean Native Rhizobium japonicum by Seroimmunological Method (토착대두근류균(土着大豆根瘤菌)의 균주(菌株) 인식(認識)과 분류(分類)를 위한 혈청면역적(血淸免疫學的) 검정(檢定))

  • Lim, Sun-Uk;Kim, Min-Kyun
    • Korean Journal of Soil Science and Fertilizer
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    • v.21 no.2
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    • pp.141-148
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    • 1988
  • Though the cultivation history of soybean in Korea is relatively long, taxonomical study on symbiont nodule bacteria, Rhizobium japonicum is not carried out yet systematically. This work was for the taxonomical study on Korean native R. japonicum by recognizing isolates seroimmunologically as well as for the elucidation of its affinity with host soybean variety. Twenty seven isolates from 13 soybean cultivars cultivated at Seoul National University's experiment field and 6 strains of R. japonicum preserved in our laboratory have been tested. Tube agglutination test, agglutinin adsorption test, and gel immune diffusion test were used. The results obtained are as follows: 1. Twenty five isolates and strains of R. japonicum among 33 were classified into 4 serogroups and identified as indivisual serotype. 2. Two isolates isolated from Hill and Milyang cultivars, 2 isolates from Bangsa and Jangbaek, and 4 isolates from Paldal, Sae-al, and Jangbaek were identified as the same serotype respectively. 3. Seroimmunological tests may be adapted for the elucidation of the affinities between the strains and soybean cultivars as well as strain recognition and systematic classification of Korean native R. japonicum.

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Evaluation of Septoria Brown Spot Disease and the Disease Resistance in Soybean Cultivars (대두(大豆)갈색무늬병의 병진전(病進展)과 품종간저항성(品種間抵抗性) 검정(檢定))

  • Oh, Jeung-Haing
    • Korean journal of applied entomology
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    • v.24 no.2 s.63
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    • pp.103-106
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    • 1985
  • The present study was carried out to determine appropriate growth stage for evaluating resistance to septoria brown spot in field and to search resistance sources from soybean germ plasm. Disease severity expressed by log $\frac{x}{1-x}$ was different with soybean genotypes and vertical progress of the disease was related to the diseased leaf area. Correlation between diseased leaf area and the area under septoria brown spot disease progress curve (AUBC) was highest at full blooming stage, indicating a reasonable stage for measuring the disease severity to evaluate resistance in field. There was no lines highly resistant to the disease among 1,428 native soybean lines tested.

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