• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.85-88
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• 2001

hGH 와 GST 절편으로 구성된 융합 단백질의 고정화 UK 칼럼에 의한 절단 반응 후 용출액의 pH를 3.5 로 낮춤으로써 이물질들을 침전시키고 이를 expanded bed chromatography 칼럼에 통과시킨 결과, 이물질들의 제거와 hGH 단량체의 흡착분리가 동시에 이루어겼다 . 흡착된 단량체는 NaCl 에 의해 용출되었으며 이 단계의 수율은 거의 100% 이었다 , 따라서 칼럼에 의한 절단 반응과 산 침전에 의한 이물질 침잔 반응 EBA 에 의한 이물질 제거 및 단량체 회수 반응을 연속적으로 진행할 수 있는 기초를 제시하였다 . 또한 고정화된 UK 는 guanidine HCl(6M)을 이용하여 unfolding 시키고 이를 세척하여 refolding 시킨 결과 20 회의 반복적인 처리 후에도 초기 활성의 약 80% 수준을 유지하였다. 이는 UK 가 공유결합된 상태에서 solid-phase refolding 이 가능하다는 증거이며, 고정화 효소 칼럼의 수영을 크게 향상시켜 경제성을 확보하는 방안으로 이용될 것으로 기대된다.

• 말소리와 음성과학
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• 제3권4호
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• pp.63-70
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• 2011

Recent studies on English $L^*$ (low pitch accent) have revealed the difference of changes in acoustic manifestation between utterances produced by Koreans and those produced by native speakers of English. However, not much effort has been made to compare $L^*$ focused constituents and non-focused constituents. At the same time, most previous works on focus realization are lacking in terms of normalization of acoustic measurement. Therefore, this research is dedicated to comparing the $L^*$ focused items and non-focused items realized by Koreans and Americans and to examining the realization of English $L^*$ produced by the two language groups with improved normalization of the acoustic features (F0, intensity and duration). Within-group analysis comparing focused words and non-focused words showed both Americans and Koreans prolonged the $L^*$ focused syllables but the effect size of syllable lengthening made by Koreans was far less than that made by Americans. Furthermore, significant F0 lowering was found in Americans but not in Koreans. However, the effect of intensity change caused by $L^*$ focus was not significant within each group. The effect of focused words was tested between the two groups revealing that Koreans implemented English $L^*$ focus with higher F0, lower intensity and shorter duration than Americans. In the instances in which a significant Group x Focus Location (initial, middle and final of a sentence) interaction was found, further analysis testing the effect of Group on each Focus Location was conducted. The testing showed that the Koreans produced shorter syllables at initial and middle of a sentence and higher F0 at initial of a sentence than Americans. Implications for the intonation training were also discussed.

• 한국환경복원기술학회지
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• 제7권3호
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• pp.35-47
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• 2004

In order to study changes in vegetation pursuant to rooftop revegetation plantation methods, plantation methods for rooftop revegetation were divided into two types through an analysis of recent trends. Then, Planted plants and invasive plants on sites where the planting methods were introduced were monitored. Planting methods were divided into mono-layer meadow cover type and multi-layer planting cover type. They showed some differences in terms of the availability of wetland, the structure of vegetation layers, the planted species, and the material of mulching. According to the results of monitoring the two sample sites for different plantation methods, the number of invasive plants was higher in multi-layer planting cover type and the ratio of naturalized plants was higher by 30% in average in mono-layer meadow cover type. The main reason for such a result is that the natural soil used in the multi-layer planting cover type likely contained some seeds. Moreover, it's harder for invasive plant seeds to germinate in volcanic rocks than in natural soil. Also, it is attributable to wetlands available in multi-layer planting cover type and diverse living environments created by multi-layer planting. The reason of the ratio of naturalized plants being higher by at least 10% in mono-layer meadow cover type is the character of naturalized plants being stronger in unfavorable conditions than nature plants are. Accordingly, the germination rate in the volcanic rock mulching has likely contributed in raising the introduction and germination of naturalized plants. The results showed that multi-layer planting cover type using wetland creation and nature soil can increase the number of invasive plants and lower the ratio of naturalized plants. However, since seeds contained in the natural soil can affect the growth of planted plants, this needs to be clarified, It was judged that mono-layer meadow cover type may affect more greatly on the germination and growth of invasive plants than on those of planted plants, Its potential adoption in highly urbanized areas was examined. By complementing with the mutual benefits of each plantation method, it appeared possible to shift to a rooftop revegetation system suitable to the site.

• 한국환경복원기술학회지
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• 제9권3호
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• pp.26-38
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• 2006

For both a healthy environment and sustainable development, we frame a habitat assessment method, concerning biodiversity and physical characteristics. With the method, we set up and extract conservation areas from Peace Valley Resort Development Areas which can function as habitats. Absolute and relative assessment items which are the size of area, habitation of species with conservation value, distribution of communities, functions of habitats, ratio of non-native plants, connectivity of habitats, vegetation layers of forests and age of forests, and water systems are considered on newly extracted conservation areas, and the habitat value assessment was calculated on the items in a 3-point scale. By comparing and examining 3 existing proposals for extracted conservation areas, we assess existing proposals, make an alternative proposal, and try to verify the possibility of applicability to extracted conservation areas. Proposal A and C must not be adopted since almost all conservation areas would be developed in the proposals. Proposal B, consisting of 53% development areas and 47% non-development areas, does not have enough development areas, so high density building arrangements should be encouraged in order to preserve conservation areas. Therefore, proposal B would be the best in ecological terms among the 3 proposals, if 3 conditions are considered : 1) new extracted conservation areas should be conserved; 2) 10~15m wide green-zones on both sides of the water system, which can play a role as ecological corridors, should be considered in development areas; 3) building arrangement should not interrupt the ecological corridors.

• Journal of Microbiology and Biotechnology
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• 제8권6호
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• pp.560-567
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• 1998

We have found that Thermoactinomyces vulgaris KFB-Cl00 produces a chitinase. The optimum temperature and pH of the enzyme activity were $55^{\circ}C$ and 6.5. The enzyme was stable after heat treatment at $80^{\circ}C$ for 30 min and stable in acidic and basic conditions (PH 6.0~11.0). The thermostable endo-chitinase from Thermoactinomyces vulgaris KFB-C100 was cloned into the plasmid pBR322 by using E. coli DH5$\alpha$ as a host strain. The positive clone carrying a recombinant plasmid (PKCHI23) with a 4.1-kb fragment containing the chitinase gene was found. The recombinant plasmid was analyzed to determine the essential region for chitinase activity and obtained a 2.3-kb fragment, which was sub cloned into pTrc99A using the PstI and SalI sites to construct pTrc99A/pKCHI23-3. The resulting plasmid exerted high chitinase activity upon transformation of E. coli XL1-Blue cells. Chitinase was overproduced 14 times more in the clone cells than in the wild-type cells and the enzyme was purified to homogeneity. The purified enzyme showed the similar properties as the native chitinase from T. vulgaris in terms of molecular weight and substrate specificity. The catalytic action of the cloned enzyme was an endo type, producing chitobiose as a major reaction product.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제39권
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• pp.13.1-13.8
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• 2017

Background: Oronasal/antral communication, loss of teeth and/or tooth-supporting bone, and facial contour deformity may occur as a consequence of maxillectomy for cancer. As a result, speaking, chewing, swallowing, and appearance are variably affected. The restoration is focused on rebuilding the oronasal wall, using either flaps (local or free) for primary closure, either prosthetic obturator. Postoperative radiotherapy surely postpones every dental procedure aimed to set fixed devices, often makes it difficult and risky, even unfeasible. Regular prosthesis, tooth-bearing obturator, and endosseous implants (in native and/or transplanted bone) are used in order to complete dental rehabilitation. Zygomatic implantology (ZI) is a valid, usually delayed, multi-staged procedure, either after having primarily closed the oronasal/antral communication or after left it untreated or amended with obturator. The present paper is an early report of a relatively new, one-stage approach for rehabilitation of patients after tumour resection, with palatal repair with loco-regional flaps and zygomatic implant insertion: supposed advantages are concentration of surgical procedures, reduced time of rehabilitation, and lowered patient discomfort. Cases presentation: We report three patients who underwent alveolo-maxillary resection for cancer and had the resulting oroantral communication directly closed with loco-regional flaps. Simultaneous zygomatic implant insertion was added, in view of granting the optimal dental rehabilitation. Conclusions: All surgical procedures were successful in terms of oroantral separation and implant survival. One patient had the fixed dental restoration just after 3 months, and the others had to receive postoperative radiotherapy; thus, rehabilitation timing was longer, as expected. We think this approach could improve the outcome in selected patients.

• 생체재료학회지
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• 제22권4호
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• pp.235-248
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• 2018

Background: Injectable hydrogels have been extensively researched for the use as scaffolds or as carriers of therapeutic agents such as drugs, cells, proteins, and bioactive molecules in the treatment of diseases and cancers and the repair and regeneration of tissues. It is because they have the injectability with minimal invasiveness and usability for irregularly shaped sites, in addition to typical advantages of conventional hydrogels such as biocompatibility, permeability to oxygen and nutrient, properties similar to the characteristics of the native extracellular matrix, and porous structure allowing therapeutic agents to be loaded. Main body: In this article, recent studies of injectable hydrogel systems applicable for therapeutic agent delivery, disease/cancer therapy, and tissue engineering have reviewed in terms of the various factors physically and chemically contributing to sol-gel transition via which gels have been formed. The various factors are as follows: several different non-covalent interactions resulting in physical crosslinking (the electrostatic interactions (e.g., the ionic and hydrogen bonds), hydrophobic interactions, ${\pi}$-interactions, and van der Waals forces), in-situ chemical reactions inducing chemical crosslinking (the Diels Alder click reactions, Michael reactions, Schiff base reactions, or enzyme-or photo-mediated reactions), and external stimuli (temperatures, pHs, lights, electric/magnetic fields, ultrasounds, or biomolecular species (e.g., enzyme)). Finally, their applications with accompanying therapeutic agents and notable properties used were reviewed as well. Conclusion: Injectable hydrogels, of which network morphology and properties could be tuned, have shown to control the load and release of therapeutic agents, consequently producing significant therapeutic efficacy. Accordingly, they are believed to be successful and promising biomaterials as scaffolds and carriers of therapeutic agents for disease and cancer therapy and tissue engineering.

• 한국항행학회논문지
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• 제23권1호
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• pp.55-60
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• 2019

contents and big data웹은 사람들의 일상생활에 있어 가장 밀접한 기술 중 하나로 오늘날 대부분의 사람들은 웹을 통해 데이터를 공유하고 있다. 단순 메신저, 뉴스, 영상뿐만 아니라 다양한 데이터가 현재 웹을 통하여 전파되고 있는 셈이다. 또한 웹 어셈블리 기술이 등장하면서 기존 네이티브 환경에서 구동되던 프로그램들이 웹의 영역에 진입하기 시작하면서 웹이 공유하는 데이터는 이제 VR/AR 콘텐츠, 빅데이터 등 그 범주가 점차 넓어지고, 크기가 거대해지고 있다. 따라서 본 논문에서는 브라우저에 종속적이지 않고 독립적으로 동작이 가능한 서비스워커와 웹 브라우저 내에 데이터를 효과적으로 저장할 수 있는 캐시 API를 활용하여 웹 서비스를 사용하는 사용자들에게 웹 콘텐츠를 효과적으로 전달할 수 있는 방법을 제시하였다.

• 아시아여성연구
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• 제53권2호
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• pp.75-110
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• 2014

이 연구의 목적은 결혼이주여성들의 새로운 직업인 통번역사의 창출 배경과 노동 경험을 분석하여, 통번역사 노동이 가진 에스닉 노동의 성격을 밝히는 것이다. 연구의 주요 결과인 통번역사의 에스닉화된 노동의 성격은 다음과 같다. 첫째, 결혼이주여성 통번역사의 창출 배경은 차별배제적 성별화된 한국 이주정책의 특성에서 찾을 수 있다. 외국인 노동자들에게는 정주가 부인되고 취업이 제한되는 반면, 결혼이주여성은 한국 가족에의 편입을 전제로 한 포섭과 지원의 대상이기 때문에 통번역사로 고용될 수 있는 것이다. 둘째, 통번역사라는 직업은 이주노동시장에서 비교적 좋은 일자리로 인식되지만, 실제 노동조건은 한국 노동시장 내에서 저임금과 불안정 고용을 특징으로 하는 에스닉 층화구조의 하층에 위치하고 있다. 셋째, 결혼이주여성 통번역사들은 이주노동자와 결혼이민자들에게 자신의 이주 경험과 언어 자원을 서비스로 제공하는 중립적 통역자이기도 하지만, 실제 노동과정에서 선주민의 차별과 불신을 경험하면서 그들의 대변인으로서의 역할과 자신 또한 이주여성이라는 정체성 사이에서 갈등을 겪는다. 결론에서는 직업으로서의 결혼이주여성 통번역사의 지속 가능성을 결혼이주여성의 임파워먼트와 노동시장 전망에 대한 쟁점을 중심으로 논의한다.

• 한국축산식품학회지
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• 제44권3호
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• pp.662-683
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• 2024

Thai-Native×Anglo-Nubian goat meat cooked by grilling (GR), sous vide (SV), and microwave (MW), was compared to fresh meat (Raw) in terms of flavor development. Non-volatile [i.e., free amino acids, nucleotide-related compounds, taste active values (TAVs) and umami equivalency, sugars, lipid oxidation, Maillard reaction products] and volatile compounds, were investigated. Notably, inosine monophosphate and Glu/Gln were the major compounds contributing to umami taste, as indicated by the highest TAVs in all samples. Raw had higher TAVs than cooked ones, indicating that heat-cooking removes these desirable flavor and taste compounds. This could be proportionally associated with the increase in aldehyde, ketone, and nitrogen-containing volatiles in all cooked samples. GR showed the highest thiobarbituric acid reactive substances (1.46 mg malonaldehyde/kg sample) and browning intensity (0.73), indicating the greatest lipid oxidation and Maillard reaction due to the higher temperature among all cooked samples (p<0.05). In contrast, SV and Raw exhibited similar profiles, indicating that low cooking temperatures preserved natural goat meat flavor, particularly the goaty odor. The principal component analysis biplot linked volatiles and non-volatiles dominant for each cooked sample to their unique flavor and taste. Therefore, these findings shed light on cooking method selection based on desirable flavor and preferences.