• Journal of Microbiology and Biotechnology
• /
• 제1권4호
• /
• pp.288-292
• /
• 1991

The aim of the current study is to evaluate the effects of medium compositions on the production of protease inhibitor in Streptomyces sp. SMF13. The production of protease inhibitor was counter-currently linked to extra-cellular protease, which were regulated by the culture conditions. Nitrogen source was the most critical ingredient affecting the production of protease inhibitor and protease. Carbon source was an important factor to determine the culture pH which affected very clearly the formation of protease and protease inhibitor. Inorganic phosphate inhibited the protease inhibitor production which was linked to the cell growth rate, although the optimal conditions for the production of protease inhibitor were not favouring to the cell growth.

• Biotechnology and Bioprocess Engineering:BBE
• /
• 제10권3호
• /
• pp.270-274
• /
• 2005

A reactor-scale hydrogen (H2) production via the water-gas shift reaction of carbon monoxide (CO) and water was studied using the purple nonsulfur bacterium, Rhodopseudomonas palustris P4. The experiment was conducted in a two-step process: an aerobic/chemoheterotrophic cell growth step and a subsequent anaerobic $H_2$ production step. Important parameters investigated included the agitation speed. inlet CO concentration and gas retention time. P4 showed a stable $H_2$ production capability with a maximum activity of 41 mmol $H_2$ g $cell^{-1}h^{-1}$ during the continuous reactor operation of 400 h. The maximal volumetric H2 production rate was estimated to be 41 mmol $H_2 L^{-1}h^{-1}$, which was about nine-fold and fifteen-fold higher than the rates reported for the photosynthetic bacteria Rhodospirillum rubrum and Rubrivivax gelatinosus, respectively. This is mainly attributed to the ability of P4 to grow to a high cell density with a high specific $H_2$ production activity. This study indicates that P4 has an outstanding potential for a continuous H2 production via the water-gas shift reaction once a proper bioreactor system that provides a high rate of gas-liquid mass transfer is developed.

• Asian-Australasian Journal of Animal Sciences
• /
• 제20권7호
• /
• pp.1049-1056
• /
• 2007

This study was conducted to compare the methane ($CH_4$) production estimated by in vivo (sulfur hexafluoride tracer technique ($SF_6$)) with that of two in vitro rumen simulation (RUSITEC) and gas production (IVGPT)) techniques. Four adult dry Holstein cows, aged $7.4{\pm}3.0$ years and weighing $697{\pm}70$ kg, were used for measuring methane production from five diets by the $SF_6$ technique. The experimental diets were alfalfa hay ($D_1$), corn silage + soybean meal (SBM) (910: 90, $D_2$), Italian rye grass hay +SBM (920: 80, $D_3$), rice straw +SBM (910: 90, $D_4$) and Sudan grass hay +SBM (920: 80, $D_5$). Each diet was individually fed to all 4 cows and 5 feeding studies of 17 d each were conducted to measure the methane production. In the RUSITEC, methane production was measured from triplicate vessels for each diet .In vitro gas production was measured for each of the diets in triplicate syringes. The gas produced after 24 and 48 h was recorded and gas samples were collected in vacuum vials and the methane production was calculated after correction for standard temperature and pressure (STP). Compared to the $SF_6$ technique, estimates of methane production using the RUSITEC were lower for all diets. Methane production estimated from 24 h in vitro gas production was higher (p<0.001) on $D_1$ as compared to that measured by $SF_6$, whereas on $D_2$ to $D_5$ it was lower. Compared to $SF_6$, methane production estimated from 48 h in vitro gas production was higher on all diets. However, methane estimated from the mean of the two measurement intervals (24+48 h/2) in IVGPT was very close to that of $SF_6$ (correlation 0.98), except on $D_1$. The results of our study confirmed that IVGPT is reflective of in vivo conditions, so that it could be used to generate a database on methane production potential of various ruminant diets and to examine strategies to modify methane emissions by ruminants.

• Biomolecules & Therapeutics
• /
• 제18권4호
• /
• pp.396-401
• /
• 2010

We conducted this study to investigate whether baicalin, baicalein or schizandrin significantly affect MUC5AC mucin production induced by epidermal growth factor (EGF) or phorbol ester (PMA) in human airway epithelial cells. Confluent NCI-H292 cells were pretreated with varying concentrations of baicalin, baicalein or schizandrin for 30 min and then stimulated with EGF or PMA for 24 h, respectively. MUC5AC mucin protein production was measured by ELISA. The results were as follows: (1) Baicalin was found to inhibit the production of MUC5AC mucin protein induced by both EGF and PMA. (2) Baicalein, the aglycone of baicalin, also inhibited MUC5AC mucin production. (3) Schizandrin, derived from Schizandrae Fructus, inhibited MUC5AC mucin production by the same inducers. These results suggest that baicalin, baicalein and schizandrin can regulate the production of mucin protein by directly acting on human airway epithelial cells.

• 한국약용작물학회지
• /
• 제12권5호
• /
• pp.378-383
• /
• 2004

Scopolamine and hyoscyamine are important anticholinergic compounds. To increase the productivity, we have selected various elicitors and developed culture system using a bubble column bioreactor (BCB). As the same manner of elicitation in flask cultures, the elicitors were introduced into BCB cultures and the productivity was investigated. Except the bacterial elicitor of Staphyllococcus aureus, the elicitors inhibited hyoscyamine production. In scopolamine production, the elicitors revealed different responses from the results obtained in flask cultures. The elicitors of KCl and Candida albicans less increased the production than flask cultures. However, methyl jasmonate and S. aureus showed stronger positive effects on tropane alkaloid production. In particular, S. aureus was the most effective elicitor on scopolamine production and the elicitor resulted in the highly increased production, approximately 10 times higher than the control culture.

• Tuberculosis and Respiratory Diseases
• /
• 제70권3호
• /
• pp.218-223
• /
• 2011

Background: We investigated whether curcumin and genistein affect the MUC5AC mucin production from human airway epithelial cells that is induced by phorbol 12-myristate 13-acetate (PMA) or tumor necrosis factor-${\alpha}$ (TNF-${\alpha}$). Methods: Confluent NCI-H292 cells were pretreated with each agent for 30 min and then stimulated with PMA or TNF-${\alpha}$ for 24 hours. MUC5AC mucin production was measured by an ELISA. Results: (1) Curcumin dose-dependently inhibited the production of MUC5AC mucin that was induced by PMA or TNF-${\alpha}$; (2) Genistein inhibited PMA-induced MUC5AC mucin production. However, it did not decrease TNF-${\alpha}$-induced MUC5AC mucin production. Conclusion: These results suggest that curcumin and genistein inhibit the production of airway mucin induced by PMA.

• 대한가정학회지
• /
• 제41권10호
• /
• pp.31-44
• /
• 2003

Household work is a productive activity, but it was not included in the System of National Accounts(SNA). Feminists and household economists complained this drawbacks of the SNA frequently. Finally, a Satellite Account for household production was allowed in the 1993 SNA. Korean government aimed to develop a Satellite Account for Korean household production. However, the development process u3s not efficiently peformed because of the lack of interests, relevant research, and appropriate dat3 at the national level in Korea. Therefore, this study aimed at examining to develop the Satellite Account for Korean household production in terms of its concept, method, data collection, and research topics. Several issues were discussed including methodology to value household production economically, appropriate ways for data collection, and future research directions in the topics of a Satellite Account.

• 산업공학
• /
• 제18권1호
• /
• pp.35-43
• /
• 2005

A production system in domestic shoes companies has difficulty in achieving automation and information because of insufficiency of flexibility and standardization. Particularly small and medium sized shoes companies producing by OEM have tendency to chase the given production schedule blindly without considering major factors that may affect the production. Therefore, the production schedules or the process conditions can not be optimally set and are extemporized by the experience in the past. These behaviors cause low productivity and financial loss. To maximize efficiency and productivity of the shoe-making process, we develop a simulation model based on a production system in small and medium sized shoes companies. The model has been developed using ARENA which has been demonstrated to be a powerful tool to simulate various manufacturing systems. Using the simulation model, we find out several problems for the production process, and then suggest several alternatives to improve the system.

• Journal of Biosystems Engineering
• /
• 제28권6호
• /
• pp.531-536
• /
• 2003

This study was carried out to develop a vacuum nozzle seeder for large seeds and performance was tested on seed feeding, arranging. and sowing peformance. The results of this study were as follows： The operation of feeding device of the seeder was programmed to operate a period of setting time after sowing 6 rows. The setting time was decided based on a discharged seed by the angular speed of feeding roller. The arranging accuracy of 'tuktozwa', 'hukjong' and 'chambak' was 96.4％, 95.2％ and 89.4％ respectively. The working performance was 75.6sheet/hr which was 3.8 times higher than that of manual work. An average seeding rate of 1 grain was 97.8％.

• 한국농림기상학회:학술대회논문집
• /
• 한국농림기상학회 2017년도 추계 학술발표초록집
• /
• pp.43-44
• /
• 2017