• Proceedings of the Costume Culture Conference
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• 2006.09a
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• pp.63-65
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• 2006

• Proceedings of the Costume Culture Conference
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• 2001.10a
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• pp.91-91
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• 2001

• Proceedings of the Costume Culture Conference
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• 2001.10a
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• pp.104-104
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• 2001

• Proceedings of the Costume Culture Conference
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• 2006.09a
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• pp.56-59
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• 2006

• Journal of Embryo Transfer
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• v.28 no.3
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• pp.273-280
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• 2013

The aim of this study was to establish a three dimensional (3D) culture system of endometrial cells and to examine the plasminogen activators (PAs) activity in porcine uterine. The 3D culture system in porcine endometrial cells was composed to mixture 3D gel, stromal cells and epithelial cells. The 3D culture system was used to identify normal structure search as uterine tissue and PAs expression in this study. In results, porcine endometrium epithelial cells forming a top monolayer and endometrium stromal cells developed as fibroblast-like within 3D matrix scaffold. Expression of urokinase-type PA (uPA) and tissue-type PA (tPA) were observed during the 3D culture using immunofluorescence. PA activity in 3D-cultured endometrial cells was no significant difference between the tissue type, but 2D culture system were significantly lower than in 3D-cultured endometrial cells (P<0.05). Therefore, basic system and functional aspect of 3D culture could be established with similar system of endometrium tissue. We suggest that this study was assumed applicable as baseline data to investigate mechanism between porcine uterus cells in vitro.

• Journal of Sensor Science and Technology
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• v.30 no.2
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• pp.109-113
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• 2021

In this study, an automated culture media replacement system was developed to analyze changes in the contraction characteristics of cardiomyocytes according to the state of the culture media. For the long-term storage of culture media, a Peltier refrigerator with a temperature of 5 to 8℃ was provided and a pH of 7.4 was maintained. The cell culture media of the cardiomyocytes was continuously replaced using interlocking pumps at a flow rate of 0.83 μl/h. The cardiomyocytes in which the culture media was replaced automatically demonstrated lower heartbeats per minute compared to samples in which there was no replacement. However, these cardiomyocytes moved more uniformly and produced greater displacement in one heartbeat cycle. It was observed that the sarcomere length of the cardiomyocytes increased due to the automated culture media replacement system. These cardiomyocytes were found to demonstrate better maturation compared to the control group. The maturation of cardiomyocytes was verified through staining images. The proposed automated culture media replacement system generates a uniform heart rate and improvements in contraction force. Based on the study, patient-specific drug toxicity assessments can be conducted using differentiated cardiomyocytes in induced pluripotent stem cells.

• Reproductive and Developmental Biology
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• v.32 no.3
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• pp.167-173
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• 2008

Somatic cells such as oviduct epithelial cell, uterine epithelial cell, cumulus-granulosa cell and buffalo rat river cell has been used to establish an effective culture system for bovine embryos produced in in vitro. But nitric oxide (NO) metabolites secreted from somatic cells were largely arrested the development of bovine in vitro matured/ in vitro fertilized (IVM/IVF) embryos, suggesting that NO was induced the embryonic toxic substance into culture medium. The objective of this study was to investigate whether BOEC co-culture system can ameliorate the NO-mediated oxidative stress in the culture of bovine IVM/IVF embryos. Therefore, we evaluated the developmental rate of bovine IVM/IVF embryos under BOEC co-culture system in the presence or absence of sodium nitroprusside (SNP), as a NO donor, and also detected the expression of growth factor (TGF-$\beta$, EGF and IGFBP) and apoptosis (Caspase-3, Bax and Bcl-2) genes. The supplement of SNP over 5 uM was strongly inhibited blastocyst development of bovine IVM/IVF embryos than in control and 1 uM SNP group (Table 2). The developmental rates beyond morulae stages of bovine IVM/IVF embryos co-cultured with BOEC regardless of SNP supplement (40.4% in 5 uM SNP+ BOEC group and 65.1% in BOEC group) were significantly increased than those of control (35.0%) and SNP single treatment group (23.3%, p<0.05: Table 3). The transcripts of Bax and Caspase-3 genes were detected in all experiment groups (1:Isolated fresh cell (IFC), 2:Primary culture cell (PCC), 3:PCC after using the embryo culture, 4: PCC containing 5 uM SNP and 5: PCC containing 5 uM SNP after using the embryo culture), but Bcl-2 gene was not detected in IFC and PCC (Fig. 1). In the expression of growth factor genes, TGF-$\beta$ gene was found in all experimental groups, and EGF and IGFBP genes were not found in IFC and PCC (Fig. 2). These results indicate that BOEC co-culture system can increase the development beyond morula stages of bovine IVM/IVF embryos, possibly suggesting the alleviation of embryonic toxic substance like nitric oxide.

• Journal of Fisheries and Marine Sciences Education
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• v.26 no.5
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• pp.1165-1174
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• 2014

The research was conducted on 238 marine sports participants by clarifying the relationship among self-efficacy, sociality, and organization culture, to eliminate the organizational(home, school, club, workplace, etc.) culture maladjustment phenomenon which is caused by personal stress, the lack of physical activity, and the lack of sociality due to the rapid change of modern society and enhance sociality, adjustment to society and the lack of creativity due to the rigid hierarchy and contribute to organization culture through marine sports among sports which we enjoy with nature. Firstly, according to general self-efficacy, and social characteristics of the organizational culture and the gender differences in higher than women in all sub-variables of the sub-factors of self-efficacy and self-regulation, sociability, organizational culture, sub-culture and develop cultural factors agreed man showed that in the sub-factors of organization culture showed that the development of high culture and hierarchical culture in the age of 20s. Secondly, in terms of the effect of self-efficacy of marine sports participants on sociality, it is found that self-regulation and level of difficulty positively influence on the culture of agreement, the culture of development and the culture of hierarchy. Lastly, in terms of the effect of self-efficacy of marine sports participants on organizational culture, it is shown that self-regulation has positive influence on the culture of agreement, the culture of development and the culture of hierarchy.

• ALGAE
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• v.22 no.3
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• pp.229-234
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• 2007

This study was to determine the extent of bacteria contamination and resistance to various antibiotics used commonly in microalgal culture. Seven different dose levels of chloramphenicol, dihydrostreptomycin sulphate, neomycin, penicillin G, streptomycin sulphate, penicillin G + streptomycin sulphate, and penicillin G + streptomycin sulphate + chloramphenicol were added to each culture of microalgae. The lethal effects on microalgae and bacteria were the highest in chloramphenicol and the lowest in penicillin G. The axenic culture of bacillariophyceae and dinophyceae was more difficult than that of chlorophyceae and haptophyceae because of their complicate external morphology. The efficient antibiotics and their concentrations for axenic cultures varied with microalgal species. The optimum quantity for antibiotic treatments were 2,000 ppm of dihydrostreptomycin for Chlorella ellipsoidea, neomycin 500 ppm of Isochrysis galbana and Heterosigma ahashiwo, hloramphenicol 500 ppm of Cyclotella didymus, and dihydrostreptomycin sulphate and neomycin 6,000 ppm of Thalassiosira allenii.

• Journal of Technology Innovation
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• v.10 no.1
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• pp.38-64
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• 2002

This paper presents research linking national culture and innovation. The research identifies how key features of Korean culture, collectivism and hierarchical authoritarianism, affect technological innovation. This perspective casts new light on cultural research that, while generally confirming national culture-innovation ties at the organization level, has had less success accounting for culture barriers to innovation in collectivist nations, especially at the interorganizational level. The data for this study was obtained from interviews and written sources. The paper introduces the concept of the social shaping of technology, with which the influence of culture on technological innovation is analyzed, and highlights the development of EDI systems in the collectivist Korean society. It draws attention to the interorganizational basis of collectivism and authoritarianism in the technology innovation process. Finally, Korean innovation systems are questioned, and some negative effects of the collectivist Korean culture at the interorganizational level are discussed.