Journal of Sensor Science and Technology (센서학회지)

The Korean Sensors Society (한국센서학회)
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A study on the maturation of cardiomyocytes by continuous supply of culture media

세포 배양액의 연속 공급기 제작을 통한 심근세포의 성숙개선에 관한 연구

  • Kwon, WooJin (School of Mechanical Engineering, Chonnam National Unversity) ;
  • Kim, Geun Woo (School of Mechanical Engineering, Chonnam National Unversity) ;
  • Jeong, Unseon (School of Mechanical Engineering, Chonnam National Unversity) ;
  • Kim, Jongyun (Graduate School of Mechanical Engineering, Seoul National Unversity) ;
  • Lee, Dong-Weon (School of Mechanical Engineering, Chonnam National Unversity)
  • 권우진 (전남대학교 기계공학부) ;
  • 김근우 (전남대학교 기계공학부) ;
  • 정운선 (전남대학교 기계공학부) ;
  • 김종윤 (전남대학교 대학원 기계공학과) ;
  • 이동원 (전남대학교 기계공학부)
  • Received : 2021.03.19
  • Accepted : 2021.03.27
  • Published : 2021.03.31
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Abstract

In this study, an automated culture media replacement system was developed to analyze changes in the contraction characteristics of cardiomyocytes according to the state of the culture media. For the long-term storage of culture media, a Peltier refrigerator with a temperature of 5 to 8℃ was provided and a pH of 7.4 was maintained. The cell culture media of the cardiomyocytes was continuously replaced using interlocking pumps at a flow rate of 0.83 μl/h. The cardiomyocytes in which the culture media was replaced automatically demonstrated lower heartbeats per minute compared to samples in which there was no replacement. However, these cardiomyocytes moved more uniformly and produced greater displacement in one heartbeat cycle. It was observed that the sarcomere length of the cardiomyocytes increased due to the automated culture media replacement system. These cardiomyocytes were found to demonstrate better maturation compared to the control group. The maturation of cardiomyocytes was verified through staining images. The proposed automated culture media replacement system generates a uniform heart rate and improvements in contraction force. Based on the study, patient-specific drug toxicity assessments can be conducted using differentiated cardiomyocytes in induced pluripotent stem cells.

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References

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